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  1. Article ; Online: Development of Improved DNA Collection and Extraction Methods for Handled Documents.

    Morgan, Ashley G / Prinz, Mechthild

    Genes

    2023  Volume 14, Issue 3

    Abstract: Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were ...

    Abstract Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were sampled with flocked swabs, cutting, and dry vacuuming. In addition, two extraction methods were compared for the sample type. DNA yields were low across all methods; however, this work confirms the ability to recover DNA from paper and the usefulness of the vacuum sampling method combined with the Chelex-Tween method. Stability of touch DNA deposits were compared over an 11-month period to better understand degradation that may occur over time. No significant difference in DNA recovery was observed, suggesting DNA deposits on paper are stable over an 11-month span.
    MeSH term(s) DNA Fingerprinting ; Crime ; Touch ; DNA/genetics ; Specimen Handling/methods
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2023-03-21
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14030761
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Development of Improved DNA Collection and Extraction Methods for Handled Documents

    Morgan, Ashley G. / Prinz, Mechthild

    Genes (Basel). 2023 Mar. 21, v. 14, no. 3

    2023  

    Abstract: Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were ...

    Abstract Handwritten documents may contain probative DNA, but most crime laboratories do not process this evidence. DNA recovery should not impair other evidence processing such as latent prints or indented writing. In this study, single fingermarks on paper were sampled with flocked swabs, cutting, and dry vacuuming. In addition, two extraction methods were compared for the sample type. DNA yields were low across all methods; however, this work confirms the ability to recover DNA from paper and the usefulness of the vacuum sampling method combined with the Chelex-Tween method. Stability of touch DNA deposits were compared over an 11-month period to better understand degradation that may occur over time. No significant difference in DNA recovery was observed, suggesting DNA deposits on paper are stable over an 11-month span.
    Keywords DNA ; crime ; paper
    Language English
    Dates of publication 2023-0321
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes14030761
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Global survey on evaluative reporting on DNA evidence with regard to activity-level propositions.

    Prinz, Mechthild / Pirtle, Devyn / Oldoni, Fabio

    Journal of forensic sciences

    2024  Volume 69, Issue 3, Page(s) 798–813

    Abstract: For many criminal cases, the source of who deposited the DNA is not what the prosecutor and the defense are trying to dispute. In court, the question may be how the DNA was deposited at the crime scene rather than who the DNA came from. Although ... ...

    Abstract For many criminal cases, the source of who deposited the DNA is not what the prosecutor and the defense are trying to dispute. In court, the question may be how the DNA was deposited at the crime scene rather than who the DNA came from. Although laboratories in many countries have begun to evaluate DNA evidence given formal activity-level propositions (ALPs), it is unknown how much other forensic practitioners know and what they think about activity-level evaluative reporting (ALR). To collect this information, a survey with 21 questions was submitted to international forensic science organizations across Europe, Australia, South America, Canada, Asia, and Africa. The survey combined open-ended and multiple-choice questions and received 162 responses. Responses revealed a wide range of knowledge on the topic. Overall, most respondents were somewhat knowledgeable about ALR, ALP, and current practices in court and expressed their support of the concept. A majority of participants identified gaps and obstacles regarding ALR they would like to see addressed. Examples include (1) need for more education/training at all stakeholder levels, (2) need for more DNA evidence-related data under realistic case scenarios, (3) need to internally implement and validate a formalized and objective approach for reporting, and (4) in some countries the need to achieve court admissibility. This global survey gathered the current concerns of forensic DNA practitioners and outlined several operational concerns. The information can be used to advance the implementation of ALR in laboratories and court testimony worldwide.
    MeSH term(s) Humans ; Surveys and Questionnaires ; DNA Fingerprinting ; DNA
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2024-02-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 219216-0
    ISSN 1556-4029 ; 0022-1198
    ISSN (online) 1556-4029
    ISSN 0022-1198
    DOI 10.1111/1556-4029.15488
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  4. Book ; Thesis: Experimentelle Untersuchungen zu Einflußfaktoren auf die Extrahierbarkeit von Desoxyribonukleinsäure (DNA) aus Blutseren

    Prinz, Mechthild

    1991  

    Author's details vorgelegt von Mechthild Prinz
    Size 132 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Ulm, Univ., Diss., 1991
    HBZ-ID HT004160283
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    92 D 2126 order for loan Magazin

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  5. Article ; Online: American forensic DNA practitioners' opinion on activity level evaluative reporting.

    Yang, Yoon Jung / Prinz, Mechthild / McKiernan, Heather / Oldoni, Fabio

    Journal of forensic sciences

    2022  Volume 67, Issue 4, Page(s) 1357–1369

    Abstract: The technical advancements made in DNA profiling now allow for very low DNA amounts to be analyzed. Accordingly, the argument often made in criminal courts is not who the DNA belongs to but rather how it was deposited. Despite the complexity of the ... ...

    Abstract The technical advancements made in DNA profiling now allow for very low DNA amounts to be analyzed. Accordingly, the argument often made in criminal courts is not who the DNA belongs to but rather how it was deposited. Despite the complexity of the relevant DNA transfer, persistence, prevalence, and recovery issues, forensic laboratories in some European countries have used evaluative reports with activity level propositions, while this is not current practice in the United States. The purpose of this study was to gain an overview of the opinions about activity level reporting (ALR) held by forensic biologists in the United States. A seventeen-question survey was distributed to members of the American Society of Crime Laboratory Directors and U.S. members of the International Society for Forensic Genetics. The survey included multiple-choice and open-response questions and received 54 responses. The majority of responses expressed moderate support of ALR. Participants mentioned six major concerns to be addressed prior to implementing ALR in the United States: (1) effect of number of variables involved; (2) need of education for practitioners/legal system; (3) inadequate number of activity studies with realistic scenarios; (4) difficulty of achieving admissibility in court; (5) need for standardized approaches/guidelines; and (6) requisite shift in perspective as to the validity of ALR. Overall, this small segment of U.S. forensic DNA practitioners appear to be willing to implement ALR once these concerns are fully addressed and resolved. As a follow-up, it would be worthwhile exploring these and other questions with a larger group and also other disciplines.
    MeSH term(s) Crime ; Criminals ; DNA/genetics ; DNA Fingerprinting ; Forensic Genetics ; Humans ; United States
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2022-05-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 219216-0
    ISSN 1556-4029 ; 0022-1198
    ISSN (online) 1556-4029
    ISSN 0022-1198
    DOI 10.1111/1556-4029.15063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Non-destructive DNA recovery from handwritten documents using a dry vacuum technique.

    McLaughlin, Patrick / Hopkins, Christian / Springer, Eliot / Prinz, Mechthild

    Journal of forensic sciences

    2021  Volume 66, Issue 4, Page(s) 1443–1451

    Abstract: Investigations of many crimes such as robberies, kidnappings, and terrorism are often associated with the recovery of a paper document which has been written by the perpetrator. Paper can provide a variety of forensic evidence such as DNA, latent ... ...

    Abstract Investigations of many crimes such as robberies, kidnappings, and terrorism are often associated with the recovery of a paper document which has been written by the perpetrator. Paper can provide a variety of forensic evidence such as DNA, latent fingermarks, and indented writing. The focus of this study was DNA recovery from handwritten notes through a vacuum suction device while preserving the other evidence types and the integrity of the document. Copy paper was used to create handwritten documents and sheets with deliberate fingerprints, and indentations. The homemade vacuum device consists of a glass pipette blocked with a moistened swab and attached to a vacuum source. The method collected sufficient DNA amounts for DNA typing analysis with 80% of the 11 copy paper samples tested giving probative DNA profiles with five being eligible for DNA database entry. DNA recovery was also tested on other commonly encountered paper types. DNA quantities would have been sufficient for STR typing for approximately 50% of manila envelopes and notebook paper samples, but not for magazine pages and bank deposit slips. Deliberate sebaceous and eccrine latent fingermarks placed onto copy paper and developed with magnetic fingerprint developer or 1,2 indanedione were not affected by the vacuum swabbing technique. Simulated robbery notes with indented writing and processed using an Electrostatic Detection Apparatus (ESDA) demonstrated no interference through the DNA collection. This vacuum-based collection method enables laboratories to reverse the current questioned document workflow and start with DNA collection.
    Language English
    Publishing date 2021-03-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 219216-0
    ISSN 1556-4029 ; 0022-1198
    ISSN (online) 1556-4029
    ISSN 0022-1198
    DOI 10.1111/1556-4029.14696
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  7. Article ; Online: Latent, genetic, and molecular genetic structure of the Wisconsin Card Sorting Test.

    Nikolašević, Željka / Bugarski Ignjatović, Vojislava / Kodžopeljić, Jasmina / Sadiković, Selka / Milovanović, Ilija / Vučinić, Nataša / Prinz, Mechthild / Budimlija, Zoran / Smederevac, Snežana

    Neuropsychology

    2022  Volume 36, Issue 4, Page(s) 314–329

    Abstract: Objective: The main goal of this study was to explore the latent structure and genetic basis of cognitive processes involved in the Wisconsin Card Sorting Task (WCST) within phenotypic, behavioral genetic, and molecular genetic research paradigms.: ... ...

    Abstract Objective: The main goal of this study was to explore the latent structure and genetic basis of cognitive processes involved in the Wisconsin Card Sorting Task (WCST) within phenotypic, behavioral genetic, and molecular genetic research paradigms.
    Method: The sample used in phenotypic and behavioral genetic analyses comprised 468 twins (154 monozygotic and 80 dizygotic twin pairs), while molecular genetic analyses were performed on 404 twins from the same sample. The zygosity of most twin pairs (96.8%) was determined via deoxyribonucleic acid (DNA) analysis of buccal swabs. Trained researchers administered the Wisconsin Card Sorting Test (WCST; Heaton et al., 1993) to the entire sample.
    Results: A phenotypic factor analysis of WCST variables suggested a single-factor solution. Overall heritability ranged from 0.19 to 0.23 across different measures of the WCST. The presence of a single general genetic factor, which could be identified from different measures of the WCST, indicated the unity of various WCST indicators and the existence of a common basic ability. Performance on the WCST did not reveal significant differences between the three genotypes on catechol-
    Conclusions: This study highlighted similarities in the phenotypic and genetic structures of the WCST, suggesting one general factor underlying different cognitive functions. The
    MeSH term(s) Brain-Derived Neurotrophic Factor/genetics ; Catechol O-Methyltransferase/genetics ; Genetic Structures ; Humans ; Neuropsychological Tests ; Wisconsin Card Sorting Test
    Chemical Substances Brain-Derived Neurotrophic Factor ; Catechol O-Methyltransferase (EC 2.1.1.6)
    Language English
    Publishing date 2022-03-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1042412-x
    ISSN 1931-1559 ; 0894-4105
    ISSN (online) 1931-1559
    ISSN 0894-4105
    DOI 10.1037/neu0000800
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: High-throughput seminal fluid identification by automated immunoaffinity mass spectrometry.

    Davidovics, Rachel / Saw, Yih Ling / Brown, Catherine O / Prinz, Mechthild / McKiernan, Heather E / Danielson, Phillip B / Legg, Kevin M

    Journal of forensic sciences

    2022  Volume 67, Issue 3, Page(s) 1184–1190

    Abstract: The identification of semen during a criminal investigation may be a critical component in the prosecution of a sexual assault. Commonly employed enzymatic and affinity-based methods for detection lack specificity, are time-consuming, and only provide a ... ...

    Abstract The identification of semen during a criminal investigation may be a critical component in the prosecution of a sexual assault. Commonly employed enzymatic and affinity-based methods for detection lack specificity, are time-consuming, and only provide a presumptive indication that semen is present where microscopic visualization is unable to meet the throughput demands. Contrary to traditional approaches, protein mass spectrometry provides true confirmatory results, but multiday sample preparation and nanoflow sample separation requirements have limited the practical applicability of these approaches. Aiming at streamlining sexual assault screening by mass spectrometry, the work here coupled a 60-minute rapid tryptic digestion, semenogelin-II peptide affinity purification on an Agilent AssayMap Bravo automation platform, and a 3-minute targeted LC-MS/MS method on an Agilent 6495 triple quadrupole mass spectrometer operating in multiple reaction monitoring mode for detecting semenogelin-II peptides in sexual assault samples. The developed assay was assessed using casework-type samples and was successful in detecting trace levels (0.0001 μl) of semen recovered from both cotton and vaginal swabs, as well as semen recovered from vaginal swabs during menses or adulterated with personal lubricants. This work represents a promising technique for high-throughput seminal fluid identification in sexual assault-type samples by mass spectrometry.
    MeSH term(s) Body Fluids ; Chromatography, Liquid ; Female ; Humans ; Peptides ; Proteins ; Tandem Mass Spectrometry
    Chemical Substances Peptides ; Proteins
    Language English
    Publishing date 2022-01-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 219216-0
    ISSN 1556-4029 ; 0022-1198
    ISSN (online) 1556-4029
    ISSN 0022-1198
    DOI 10.1111/1556-4029.14975
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  9. Article: Quantitative behavioral genetic and molecular genetic foundations of the approach and avoidance strategies.

    Smederevac, Snežana / Sadiković, Selka / Čolović, Petar / Vučinić, Nataša / Milutinović, Aleksandra / Riemann, Rainer / Corr, Philip J / Prinz, Mechthild / Budimlija, Zoran

    Current psychology (New Brunswick, N.J.)

    2022  , Page(s) 1–15

    Abstract: Two studies examined genetic and environmental influences on traits proposed by the revised Reinforcement Sensitivity Theory (rRST) of personality. Both quantitative and molecular behavioral genetic methods were applied considering the effects ... ...

    Abstract Two studies examined genetic and environmental influences on traits proposed by the revised Reinforcement Sensitivity Theory (rRST) of personality. Both quantitative and molecular behavioral genetic methods were applied considering the effects of
    Language English
    Publishing date 2022-01-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2021598-8
    ISSN 1936-4733 ; 1046-1310
    ISSN (online) 1936-4733
    ISSN 1046-1310
    DOI 10.1007/s12144-022-02724-9
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  10. Article ; Online ; Conference proceedings: Highlights of the 25th Congress of the International Society for Forensic Genetics (ISFG).

    Prinz, Mechthild / Linacre, Adrian

    Forensic science international. Genetics

    2014  Volume 8, Issue 1, Page(s) 244–245

    MeSH term(s) Forensic Genetics ; Internationality ; Societies, Medical
    Language English
    Publishing date 2014-01
    Publishing country Netherlands
    Document type Congresses ; Editorial
    ZDB-ID 2493339-9
    ISSN 1878-0326 ; 1872-4973
    ISSN (online) 1878-0326
    ISSN 1872-4973
    DOI 10.1016/j.fsigen.2013.10.013
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