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  1. Article ; Online: Cell cycle dependence of cell survival following exposure to X-rays in synchronous HeLa cells expressing fluorescent ubiquitination-based cell cycle indicators.

    Seino, Ryosuke / Uno, Hiroto / Prise, Kevin M / Fukunaga, Hisanori

    Biomedical research (Tokyo, Japan)

    2024  Volume 45, Issue 1, Page(s) 25–31

    Abstract: The cell cycle dependence of radiosensitivity has yet to be fully determined, as it is technically difficult to achieve a high degree of cell cycle synchronization in cultured cell systems and accurately detect the cell cycle phase of individual cells ... ...

    Abstract The cell cycle dependence of radiosensitivity has yet to be fully determined, as it is technically difficult to achieve a high degree of cell cycle synchronization in cultured cell systems and accurately detect the cell cycle phase of individual cells simultaneously. We used human cervical carcinoma HeLa cells expressing fluorescent ubiquitination-based cell cycle indicators (FUCCI), and employed the mitotic harvesting method that is one of the cell cycle synchronization methods. The imaging analysis confirmed that the cell cycle is highly synchronized after mitotic cell harvesting until 18-20 h of the doubling time has elapsed. Also, flow cytometry analysis revealed that the S and G2 phases peak at approximately 12 and 14-16 h, respectively, after mitotic harvesting. In addition, the clonogenic assay showed the changes in surviving fractions following exposure to X-rays according to the progress through the cell cycle. These results indicate that HeLa-FUCCI cells become radioresistant in the G1 phase, become radiosensitive in the early S phase, rapidly become radioresistant in the late S phase, and become radiosensitive again in the G2 phase. Our findings may contribute to the further development of combinations of radiation and cell cycle-specific anticancer agents.
    MeSH term(s) Humans ; HeLa Cells ; X-Rays ; Cell Survival ; Microscopy, Fluorescence ; Cell Cycle ; Ubiquitination
    Language English
    Publishing date 2024-02-07
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 604561-3
    ISSN 1880-313X ; 0388-6107
    ISSN (online) 1880-313X
    ISSN 0388-6107
    DOI 10.2220/biomedres.45.25
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: 10 years of open access publishing at the BIR.

    Jackson, Simon A / Prise, Kevin M

    The British journal of radiology

    2021  Volume 95, Issue 1129, Page(s) 20229001

    Language English
    Publishing date 2021-12-01
    Publishing country England
    Document type Editorial
    ZDB-ID 2982-8
    ISSN 1748-880X ; 0007-1285
    ISSN (online) 1748-880X
    ISSN 0007-1285
    DOI 10.1259/bjr.20229001
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  3. Article ; Online: Investigating the potential contribution of inter-track interactions within ultra-high dose-rate proton therapy.

    Thompson, Shannon J / Prise, Kevin M / McMahon, Stephen J

    Physics in medicine and biology

    2023  Volume 68, Issue 5

    Abstract: ... ...

    Abstract Objective
    MeSH term(s) Proton Therapy/methods ; Protons ; Monte Carlo Method
    Chemical Substances Protons
    Language English
    Publishing date 2023-02-20
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208857-5
    ISSN 1361-6560 ; 0031-9155
    ISSN (online) 1361-6560
    ISSN 0031-9155
    DOI 10.1088/1361-6560/acb88a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 199: Show issues Location:
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  4. Article: A Brief Overview of Radiation-Induced Effects on Spermatogenesis and Oncofertility.

    Fukunaga, Hisanori / Yokoya, Akinari / Prise, Kevin M

    Cancers

    2022  Volume 14, Issue 3

    Abstract: The genotoxicity of radiation on germ cells may be passed on to the next generation, thus its elucidation is not only a scientific issue but also an ethical, legal, and social issue in modern society. In this article, we briefly overview the effects of ... ...

    Abstract The genotoxicity of radiation on germ cells may be passed on to the next generation, thus its elucidation is not only a scientific issue but also an ethical, legal, and social issue in modern society. In this article, we briefly overview the effects of radiation on spermatogenesis and its associated genotoxicity, including the latest findings in the field of radiobiology. The potential role of transgenerational effects is still poorly understood, and further research in this area is desirable. Furthermore, from the perspective of oncofertility, we discuss the historical background and clinical importance of preserving male fertility during radiation treatment and the potential of microbeam radiotherapy. We hope that this review will contribute to stimulating further discussions and investigations for therapies for pediatric and adolescent/young adult patients.
    Language English
    Publishing date 2022-02-04
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers14030805
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  5. Article ; Online: 125 years of

    Jackson, Simon A / Prise, Kevin M

    The British journal of radiology

    2020  Volume 94, Issue 1117, Page(s) bjr20219001

    Language English
    Publishing date 2020-12-11
    Publishing country England
    Document type Editorial
    ZDB-ID 2982-8
    ISSN 1748-880X ; 0007-1285
    ISSN (online) 1748-880X
    ISSN 0007-1285
    DOI 10.1259/bjr.20219001
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  6. Article ; Online: High-LET radiation induces large amounts of rapidly-repaired sublethal damage.

    Guerra Liberal, Francisco D C / Thompson, Shannon J / Prise, Kevin M / McMahon, Stephen J

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 11198

    Abstract: There is agreement that high-LET radiation has a high Relative Biological Effectiveness (RBE) when delivered as a single treatment, but how it interacts with radiations of different qualities, such as X-rays, is less clear. We sought to clarify these ... ...

    Abstract There is agreement that high-LET radiation has a high Relative Biological Effectiveness (RBE) when delivered as a single treatment, but how it interacts with radiations of different qualities, such as X-rays, is less clear. We sought to clarify these effects by quantifying and modelling responses to X-ray and alpha particle combinations. Cells were exposed to X-rays, alpha particles, or combinations, with different doses and temporal separations. DNA damage was assessed by 53BP1 immunofluorescence, and radiosensitivity assessed using the clonogenic assay. Mechanistic models were then applied to understand trends in repair and survival. 53BP1 foci yields were significantly reduced in alpha particle exposures compared to X-rays, but these foci were slow to repair. Although alpha particles alone showed no inter-track interactions, substantial interactions were seen between X-rays and alpha particles. Mechanistic modelling suggested that sublethal damage (SLD) repair was independent of radiation quality, but that alpha particles generated substantially more sublethal damage than a similar dose of X-rays, [Formula: see text]. This high RBE may lead to unexpected synergies for combinations of different radiation qualities which must be taken into account in treatment design, and the rapid repair of this damage may impact on mechanistic modelling of radiation responses to high LETs.
    MeSH term(s) Radiation, Ionizing ; Alpha Particles ; Biological Assay ; DNA Damage ; Radiation Tolerance
    Language English
    Publishing date 2023-07-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-38295-3
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  7. Article ; Online: 125 years of

    Jackson, Simon A / Prise, Kevin M

    The British journal of radiology

    2019  Volume 93, Issue 1105, Page(s) 20209001

    Language English
    Publishing date 2019-12-31
    Publishing country England
    Document type Editorial
    ZDB-ID 2982-8
    ISSN 1748-880X ; 0007-1285
    ISSN (online) 1748-880X
    ISSN 0007-1285
    DOI 10.1259/bjr.20209001
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    In stock of ZB MED Cologne/Königswinter

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  8. Article: Synergistic Activity of DNA Damage Response Inhibitors in Combination with Radium-223 in Prostate Cancer.

    Dunne, Victoria L / Wright, Timothy C / Liberal, Francisco D C Guerra / O'Sullivan, Joe M / Prise, Kevin M

    Cancers

    2024  Volume 16, Issue 8

    Abstract: Radium-223 ( ...

    Abstract Radium-223 (
    Language English
    Publishing date 2024-04-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers16081510
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  9. Article: A Mechanistic DNA Repair and Survival Model (Medras): Applications to Intrinsic Radiosensitivity, Relative Biological Effectiveness and Dose-Rate.

    McMahon, Stephen Joseph / Prise, Kevin M

    Frontiers in oncology

    2021  Volume 11, Page(s) 689112

    Abstract: Variations in the intrinsic radiosensitivity of different cells to ionizing radiation is now widely believed to be a significant driver in differences in response to radiotherapy. While the mechanisms of radiosensitivity have been extensively studied in ... ...

    Abstract Variations in the intrinsic radiosensitivity of different cells to ionizing radiation is now widely believed to be a significant driver in differences in response to radiotherapy. While the mechanisms of radiosensitivity have been extensively studied in the laboratory, there are a lack of models which integrate this knowledge into a predictive framework. This paper presents an overview of the Medras model, which has been developed to provide a mechanistic framework in which different radiation responses can be modelled and individual responses predicted. This model simulates the repair of radiation-induced DNA damage, incorporating the overall kinetics of repair and its fidelity, to predict a range of biological endpoints including residual DNA damage, mutation, chromosome aberration, and cell death. Validation of this model against a range of exposure types is presented, including considerations of varying radiation qualities and dose-rates. This approach has the potential to inform new tools to deliver mechanistic predictions of radiation sensitivity, and support future developments in treatment personalization.
    Language English
    Publishing date 2021-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2649216-7
    ISSN 2234-943X
    ISSN 2234-943X
    DOI 10.3389/fonc.2021.689112
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  10. Article ; Online: Non-uniform radiation-induced biological responses at the tissue level involved in the health risk of environmental radiation: a radiobiological hypothesis.

    Fukunaga, Hisanori / Prise, Kevin M

    Environmental health : a global access science source

    2018  Volume 17, Issue 1, Page(s) 93

    Abstract: Background: The conventional concept of radiation protection is based on epidemiological studies of radiation that support a positive correlation between dose and response. However, there is a remarkable difference in biological responses at the tissue ... ...

    Abstract Background: The conventional concept of radiation protection is based on epidemiological studies of radiation that support a positive correlation between dose and response. However, there is a remarkable difference in biological responses at the tissue level, depending on whether radiation is delivered as a uniform or non-uniform spatiotemporal distribution due to tissue sparing effects (TSE). From the point of view of radiation micro-dosimetry, environmental radiation is delivered as a non-uniform distribution, and radiation-induced biological responses at the tissue level, such as TSE, would be implicated in individual risk following exposure to environmental radiation.
    Hypothesis: We hypothesize that the health risks of non-uniform radiation exposure are lower than the same dose at a uniform exposure, due to TSE following irradiation. Testing the hypothesis requires both radiobiological studies using high-precision microbeams and the epidemiological data of environmental radiation-induced effects. The implications of the hypothesis will lead to more personalized approaches in the field of environmental radiation protection.
    Conclusion: The detection of spatiotemporal dose distribution could be of scientific importance for more accurate individual risk assessment of exposure to environmental radiation. Further radiobiological studies on non-uniform radiation-induced biological responses at the tissue level are expected.
    MeSH term(s) Dose-Response Relationship, Radiation ; Humans ; Radiation Exposure/adverse effects ; Radiation Injuries ; Risk Assessment
    Language English
    Publishing date 2018-12-29
    Publishing country England
    Document type Journal Article
    ISSN 1476-069X
    ISSN (online) 1476-069X
    DOI 10.1186/s12940-018-0444-4
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