Article ; Online: Keratinocytes activated by IL-4/IL-13 express IL-2Rγ with consequences on epidermal barrier function.
2023 Volume 32, Issue 5, Page(s) 660–670
Abstract: Atopic dermatitis (AD) is a Th2-type inflammatory disease characterized by an alteration of epidermal barrier following the release of IL-4 and IL-13. These cytokines activate type II IL-4Rα/IL-13Rα1 receptors in the keratinocyte. Whilst IL-2Rγ, that ... ...
| Abstract | Atopic dermatitis (AD) is a Th2-type inflammatory disease characterized by an alteration of epidermal barrier following the release of IL-4 and IL-13. These cytokines activate type II IL-4Rα/IL-13Rα1 receptors in the keratinocyte. Whilst IL-2Rγ, that forms type I receptor for IL-4, is only expressed in haematopoietic cells, recent studies suggest its induction in keratinocytes, which questions about its role. We studied expression of IL-2Rγ in keratinocytes and its role in alteration of keratinocyte function and epidermal barrier. IL-2Rγ expression in keratinocytes was studied using both reconstructed human epidermis (RHE) exposed to IL-4/IL-13 and AD skin. IL-2Rγ induction by type II receptor has been analyzed using JAK inhibitors and RHE knockout (KO) for IL13RA1. IL-2Rγ function was investigated in RHE KO for IL2RG. In RHE, IL-4/IL-13 induce expression of IL-2Rγ at the mRNA and protein levels. Its mRNA expression is also visualized in keratinocytes of lesional AD skin. IL-2Rγ expression is low in RHE treated with JAK inhibitors and absent in RHE KO for IL13RA1. Exposure to IL-4/IL-13 alters epidermal barrier, but this alteration is absent in RHE KO for IL2RG. A more important induction of IL-13Rα2 is reported in RHE KO for IL2RG than in not edited RHE. These results demonstrate IL-2Rγ induction in keratinocytes through activation of type II receptor. IL-2Rγ is involved in the alteration of the epidermal barrier and in the regulation of IL-13Rα2 expression. Observation of IL-2Rγ expression by keratinocytes inside AD lesional skin suggests a role for this receptor subunit in the disease. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Humans ; Cells, Cultured ; Dermatitis, Atopic/metabolism ; Epidermis/metabolism ; Interleukin-13/metabolism ; Interleukin-4/metabolism ; Janus Kinase Inhibitors ; Keratinocytes/metabolism ; RNA, Messenger/metabolism ; Interleukin Receptor Common gamma Subunit/metabolism | |||||
| Chemical Substances | Interleukin-13 ; Interleukin-4 (207137-56-2) ; Janus Kinase Inhibitors ; RNA, Messenger ; IL2RG protein, human ; Interleukin Receptor Common gamma Subunit | |||||
| Language | English | |||||
| Publishing date | 2023-01-26 | |||||
| Publishing country | Denmark | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 1130936-2 | |||||
| ISSN | 1600-0625 ; 0906-6705 | |||||
| ISSN (online) | 1600-0625 | |||||
| ISSN | 0906-6705 | |||||
| DOI | 10.1111/exd.14749 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 3508: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.