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  1. Article ; Online: Validating Tools to Detect and Inactivate Monkeypox Virus in Human Milk.

    Clark, Alex E / Furst, Annalee / Sejane, Kristija / Stellwagen, Lisa / Proost, Marissa / Pride, David / Smith, Davey M / Carlin, Aaron F / Bode, Lars

    Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine

    2023  Volume 18, Issue 10, Page(s) 785–789

    Abstract: Objectives: ...

    Abstract Objectives:
    MeSH term(s) Female ; Humans ; Milk, Human ; Monkeypox virus ; Breast Feeding ; Pasteurization ; Hot Temperature
    Language English
    Publishing date 2023-09-21
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2234680-6
    ISSN 1556-8342 ; 1556-8253
    ISSN (online) 1556-8342
    ISSN 1556-8253
    DOI 10.1089/bfm.2023.0175
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A simple solid media assay for detection of synergy between bacteriophages and antibiotics.

    Khong, Ethan / Oh, Joseph J / Jimenez, Julian M / Liu, Roland / Dunham, Sage / Monsibais, Alisha / Rhoads, Alison / Ghatbale, Pooja / Garcia, Andrew / Cobián Güemes, Ana Georgina / Blanc, Alisha N / Chiu, Megan / Kuo, Peiting / Proost, Marissa / Kline, Ahnika / Aslam, Saima / Schooley, Robert T / Whiteson, Katrine / Fraley, Stephanie I /
    Pride, David T

    Microbiology spectrum

    2024  Volume 12, Issue 5, Page(s) e0322123

    Abstract: The emergence of antibiotic-resistant bacteria (ARB) has necessitated the development of alternative therapies to deal with this global threat. Bacteriophages (viruses that target bacteria) that kill ARB are one such alternative. Although phages have ... ...

    Abstract The emergence of antibiotic-resistant bacteria (ARB) has necessitated the development of alternative therapies to deal with this global threat. Bacteriophages (viruses that target bacteria) that kill ARB are one such alternative. Although phages have been used clinically for decades with inconsistent results, a number of recent advances in phage selection, propagation, and purification have enabled a reevaluation of their utility in contemporary clinical medicine. In most phage therapy cases, phages are administered in combination with antibiotics to ensure that patients receive the standard-of-care treatment. Some phages may work cooperatively with antibiotics to eradicate ARB, as often determined using non-standardized broth assays. We sought to develop a solid media-based assay to assess cooperativity between antibiotics and phages to offer a standardized platform for such testing. We modeled the interactions that occur between antibiotics and phages on solid medium to measure additive, antagonistic, and synergistic interactions. We then tested the method using different bacterial isolates and identified a number of isolates where synergistic interactions were identified. These interactions were not dependent on the specific organism, phage family, or antibiotic used.
    MeSH term(s) Bacteriophages/physiology ; Bacteriophages/isolation & purification ; Anti-Bacterial Agents/pharmacology ; Phage Therapy/methods ; Humans ; Culture Media/chemistry ; Microbial Sensitivity Tests/methods ; Bacteria/virology ; Bacteria/drug effects ; Drug Resistance, Bacterial
    Chemical Substances Anti-Bacterial Agents ; Culture Media
    Language English
    Publishing date 2024-03-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.03221-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Jumbo phages are active against extensively drug-resistant eyedrop-associated

    Cobián Güemes, Ana Georgina / Ghatbale, Pooja / Blanc, Alisha N / Morgan, Chase J / Garcia, Andrew / Leonard, Jesse / Huang, Lina / Kovalick, Grace / Proost, Marissa / Chiu, Megan / Kuo, Peiting / Oh, Joseph / Karthikeyan, Smruthi / Knight, Rob / Pogliano, Joe / Schooley, Robert T / Pride, David T

    Antimicrobial agents and chemotherapy

    2023  Volume 67, Issue 12, Page(s) e0065423

    Abstract: Antibiotic-resistant bacteria present an emerging challenge to human health. Their prevalence has been increasing across the globe due in part to the liberal use of antibiotics that has pressured them to develop resistance. Those bacteria that acquire ... ...

    Abstract Antibiotic-resistant bacteria present an emerging challenge to human health. Their prevalence has been increasing across the globe due in part to the liberal use of antibiotics that has pressured them to develop resistance. Those bacteria that acquire mobile genetic elements are especially concerning because those plasmids may be shared readily with other microbes that can then also become antibiotic resistant. Serious infections have recently been related to the contamination of preservative-free eyedrops with extensively drug-resistant (XDR) isolates of
    MeSH term(s) Humans ; Bacteriophages/genetics ; Pseudomonas Infections/microbiology ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Plasmids ; Pseudomonas aeruginosa ; Pseudomonas Phages/genetics
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2023-11-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 217602-6
    ISSN 1098-6596 ; 0066-4804
    ISSN (online) 1098-6596
    ISSN 0066-4804
    DOI 10.1128/aac.00654-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article: A simple solid media assay for detection of synergy between bacteriophages and antibiotics.

    Khong, Ethan / Oh, Joseph / Jimenez, Julian M / Liu, Roland / Dunham, Sage / Monsibais, Alisha / Rhoads, Alison / Ghatbale, Pooja / Garcia, Andrew / Cobián Güemes, Ana Georgina / Blanc, Alisha N / Chiu, Megan / Kuo, Peiting / Proost, Marissa / Kline, Ahnika / Aslam, Saima / Schooley, Robert T / Whiteson, Katrine / Fraley, Stephanie I /
    Pride, David T

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The emergence of antibiotic resistant bacteria (ARB) has necessitated the development of alternative therapies to deal with this global threat. Bacteriophages (viruses that target bacteria) that kill ARB are one such alternative. While phages have been ... ...

    Abstract The emergence of antibiotic resistant bacteria (ARB) has necessitated the development of alternative therapies to deal with this global threat. Bacteriophages (viruses that target bacteria) that kill ARB are one such alternative. While phages have been used clinically for decades with inconsistent results, a number of recent advances in phage selection, propagation and purification have enabled a reevaluation of their utility in contemporary clinical medicine. In most phage therapy cases, phages are administered in combination with antibiotics to ensure that patients receive the standard-of-care treatment. Some phages may work cooperatively with antibiotics to eradicate ARB, as often determined using non-standardized broth assays. We sought to develop a solid media-based assay to assess cooperativity between antibiotics and phages to offer a standardized platform for such testing. We modeled the interactions that occur between antibiotics and phages on solid medium to measure additive, antagonistic, and synergistic interactions. We then tested the method using different bacterial isolates, and identified a number of isolates where synergistic interactions were identified. These interactions were not dependent on the specific organism, phage family, or antibiotic used.
    Language English
    Publishing date 2023-08-24
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.08.23.554535
    Database MEDical Literature Analysis and Retrieval System OnLINE

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