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Article ; Online: Candida albicans Double Mutants Lacking both

Park, Yang-Nim / Pujol, Claude / Wessels, Deborah J / Soll, David R

mSphere

2020 Volume 5, Issue 5

Abstract: ... Candida ... ...

Abstract	Candida albicans
MeSH term(s)	Animals ; Candida albicans/genetics ; DNA-Binding Proteins/genetics ; Female ; Fungal Proteins/genetics ; Gene Deletion ; Gene Expression Regulation, Fungal ; Genes, Mating Type, Fungal ; Mice, Inbred C57BL ; Mutation ; Phenotype ; Transcription Factors/genetics
Chemical Substances	DNA-Binding Proteins ; EFG1 protein, Candida albicans ; Fungal Proteins ; Transcription Factors
Language	English
Publishing date	2020-09-23
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00918-20
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Article ; Online: Role of the

Conway, Thomas P / Conway, Kayla / Boksa, Frank A / Pujol, Claude / Wessels, Deborah / Soll, David R

mBio

2021 Volume 12, Issue 5, Page(s) e0232021

Abstract: During induced differentiation, the process often involves a commitment event, after which induced cells, when returned to noninducing conditions, continue to differentiate. The commitment event is rarely identified. Candida albicans differentiates from ... ...

Abstract	During induced differentiation, the process often involves a commitment event, after which induced cells, when returned to noninducing conditions, continue to differentiate. The commitment event is rarely identified. Candida albicans differentiates from the white to opaque phenotype, a prerequisite for mating and a process accompanying colonization of the lower gastrointestinal tract and skin. In analyses of white cell populations induced to synchronously differentiate from the white to opaque phenotype, opaque commitment occurs at approximately the same time as evagination and chitin ring formation in the process of daughter cell formation, several hours after the master switch gene
MeSH term(s)	Candida albicans/genetics ; Candida albicans/growth & development ; Candida albicans/metabolism ; Color ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Fungal ; Phenotype
Chemical Substances	Fungal Proteins
Language	English
Publishing date	2021-09-07
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.02320-21
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Article ; Online: EFG1

Park, Yang-Nim / Conway, Kayla / Pujol, Claude / Daniels, Karla J / Soll, David R

mSphere

2020 Volume 5, Issue 1

Abstract: The transcription ... ...

Abstract	The transcription factor
MeSH term(s)	Animals ; Candida albicans/genetics ; Candida albicans/physiology ; DNA-Binding Proteins/genetics ; Female ; Fungal Proteins/genetics ; Gene Deletion ; Gene Expression Regulation, Fungal ; Glucose ; Mice ; Mice, Inbred C57BL ; Mutation ; Phenotype ; Transcription Factors/genetics
Chemical Substances	DNA-Binding Proteins ; EFG1 protein, Candida albicans ; Fungal Proteins ; Transcription Factors ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2020-02-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00795-19
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Article ; Online: Comparison of Switching and Biofilm Formation between MTL-Homozygous Strains of Candida albicans and Candida dubliniensis.

Pujol, Claude / Daniels, Karla J / Soll, David R

Eukaryotic cell

2015 Volume 14, Issue 12, Page(s) 1186–1202

Abstract: Candida albicans and Candida dubliniensis are highly related species that share the same main developmental programs. In C. albicans, it has been demonstrated that the biofilms formed by strains heterozygous and homozygous at the mating type locus (MTL) ... ...

Abstract	Candida albicans and Candida dubliniensis are highly related species that share the same main developmental programs. In C. albicans, it has been demonstrated that the biofilms formed by strains heterozygous and homozygous at the mating type locus (MTL) differ functionally, but studies rarely identify the MTL configuration. This becomes a particular problem in studies of C. dubliniensis, given that one-third of natural strains are MTL homozygous. For that reason, we have analyzed MTL-homozygous strains of C. dubliniensis for their capacity to switch from white to opaque, the stability of the opaque phenotype, CO2 induction of switching, pheromone induction of adhesion, the effects of minority opaque cells on biofilm thickness and dry weight, and biofilm architecture in comparison with C. albicans. Our results reveal that C. dubliniensis strains switch to opaque at lower average frequencies, exhibit a far lower level of opaque phase stability, are not stimulated to switch by high CO2, exhibit more variability in biofilm architecture, and most notably, form mature biofilms composed predominately of pseudohyphae rather than true hyphae. Therefore, while several traits of MTL-homozygous strains of C. dubliniensis appear to be degenerating or have been lost, others, most notably several related to biofilm formation, have been conserved. Within this context, the possibility is considered that C. dubliniensis is transitioning from a hypha-dominated to a pseudohypha-dominated biofilm and that aspects of C. dubliniensis colonization may provide insights into the selective pressures that are involved.
MeSH term(s)	Adhesiveness/drug effects ; Biofilms/drug effects ; Candida/cytology ; Candida/drug effects ; Candida/genetics ; Candida/physiology ; Candida albicans/cytology ; Candida albicans/drug effects ; Candida albicans/genetics ; Candida albicans/physiology ; Carbon Dioxide/pharmacology ; Genes, Mating Type, Fungal ; Genes, Switch ; Heterozygote ; Homozygote ; Hyphae/cytology ; Hyphae/drug effects ; Pheromones/pharmacology ; Vacuoles/metabolism
Chemical Substances	Pheromones ; Carbon Dioxide (142M471B3J)
Language	English
Publishing date	2015-12
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2077635-4
ISSN	1535-9786 ; 1535-9778
ISSN (online)	1535-9786
ISSN	1535-9778
DOI	10.1128/EC.00146-15
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Article: DNA fingerprinting Candida species.

Pujol, Claude / Soll, David R

Methods in molecular biology (Clifton, N.J.)

2009 Volume 499, Page(s) 117–129

Abstract: It is sometimes necessary to assess the genetic relatedness of isolates to identify the origin of an infection. In addition, evidence is accumulating that drug resistance can be associated with strains from a particular clade and that strains can exhibit ...

Abstract	It is sometimes necessary to assess the genetic relatedness of isolates to identify the origin of an infection. In addition, evidence is accumulating that drug resistance can be associated with strains from a particular clade and that strains can exhibit anatomical specificity. It may, therefore, be valuable in the near future to screen for strains with a propensity for drug resistance. While a number of methods exist for genetically fingerprinting the infectious fungi, only a few provide the necessary resolution not only for distinguishing whether strains are highly related or unrelated, but also for grouping a strain in a particular clade. Here, we provide the procedures for performing the two methods that have proven most effective in the past 5 years: Southern blot hybridization of restriction fragments with complex probes and multilocus sequence typing (MLST).
MeSH term(s)	Candida/genetics ; Candida/isolation & purification ; Candidiasis/microbiology ; DNA Fingerprinting/methods ; Humans ; Mycological Typing Techniques/methods ; Polymorphism, Restriction Fragment Length
Language	English
Publishing date	2009
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	1064-3745
ISSN	1064-3745
DOI	10.1007/978-1-60327-151-6_12
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Article ; Online: Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D.

Wessels, Deborah J / Pujol, Claude / Pradhan, Nikash / Lusche, Daniel F / Gonzalez, Luis / Kelly, Sydney E / Martin, Elizabeth M / Voss, Edward R / Park, Yang-Nim / Dailey, Michael / Sugg, Sonia L / Phadke, Sneha / Bashir, Amani / Soll, David R

Cell adhesion & migration

2021 Volume 15, Issue 1, Page(s) 224–248

Abstract: We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer ... ...

Abstract	We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer cells migrate toward HUVEC networks, facilitated by filopodia, migrate along the network surfaces, penetrate into and migrate within the HUVEC networks, exit and continue migrating along network surfaces. The system is highly amenable to 3D reconstruction and computational analyses, and assessments of the effects of potential anti-metastasis monoclonal antibodies and other drugs. We demonstrate that an anti-RHAMM antibody blocks filopodium formation and all of the behaviors that we found take place between MB-231 cells and HUVEC networks.
MeSH term(s)	Breast Neoplasms ; Cell Movement ; Female ; Human Umbilical Vein Endothelial Cells ; Humans ; Pharmaceutical Preparations ; Pseudopodia
Chemical Substances	Pharmaceutical Preparations
Language	English
Publishing date	2021-08-01
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2268518-2
ISSN	1933-6926 ; 1933-6918
ISSN (online)	1933-6926
ISSN	1933-6918
DOI	10.1080/19336918.2021.1957527
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Article ; Online: Binding Sites in the EFG1 Promoter for Transcription Factors in a Proposed Regulatory Network: A Functional Analysis in the White and Opaque Phases of Candida albicans.

Pujol, Claude / Srikantha, Thyagarajan / Park, Yang-Nim / Daniels, Karla J / Soll, David R

G3 (Bethesda, Md.)

2016 Volume 6, Issue 6, Page(s) 1725–1737

Abstract: In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed ... ...

Abstract	In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1, we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor effects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.
Language	English
Publishing date	2016-06-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2629978-1
ISSN	2160-1836 ; 2160-1836
ISSN (online)	2160-1836
ISSN	2160-1836
DOI	10.1534/g3.116.029785
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Article ; Online: Role of Tec1 in the development, architecture, and integrity of sexual biofilms of Candida albicans.

Daniels, Karla J / Srikantha, Thyagarajan / Pujol, Claude / Park, Yang-Nim / Soll, David R

Eukaryotic cell

2015 Volume 14, Issue 3, Page(s) 228–240

Abstract: MTL-homozygous ( A: / A: or α/α) white cells form a complex sexual biofilm that exhibits the same architecture as that of MTL-heterozygous ( A: /α) pathogenic biofilms. However, the former is regulated by the mitogen-activated protein (MAP) kinase ... ...

Abstract	MTL-homozygous ( A: / A: or α/α) white cells form a complex sexual biofilm that exhibits the same architecture as that of MTL-heterozygous ( A: /α) pathogenic biofilms. However, the former is regulated by the mitogen-activated protein (MAP) kinase pathway, while the latter is regulated by the Ras1/cyclic AMP (cAMP) pathway. We previously demonstrated that in the formation of an MTL-homozygous, mature (48 h) sexual biofilm in RPMI 1640 medium, the MAP kinase pathway targets Tec1 rather than Cph1, the latter of which is the target of the same pathway, but for the opaque cell mating response. Here we continued our analysis of the role of Tec1 by comparing the effects of deleting TEC1 on initial adhesion to silicone elastomer, high-resolution confocal microscopy assessments of the stages and cellular phenotypes during the 48 h of biofilm development, human white cell penetration, and biofilm fragility. We show that although Tec1 plays only a minor role in initial adhesion to the silicone elastomer, it does play a major role in the growth of the basal yeast cell polylayer, vertical extension of hyphae and matrix deposition in the upper portion of the biofilm, final biofilm thickness, penetrability of human white blood cells, and final biofilm integrity (i.e., resistance to fluid flow). These results provide a more detailed description of normal biofilm development and architecture and confirm the central role played by the transcription factor Tec1 in the biofilm model employed here.
MeSH term(s)	Amino Acid Sequence ; Biofilms ; Candida albicans/drug effects ; Candida albicans/genetics ; Candida albicans/physiology ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Genes, Mating Type, Fungal ; Molecular Sequence Data ; Silicone Elastomers/pharmacology ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	DNA-Binding Proteins ; Fungal Proteins ; Silicone Elastomers ; TEC1 protein, Candida albicans ; Transcription Factors
Language	English
Publishing date	2015-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	2077635-4
ISSN	1535-9786 ; 1535-9778
ISSN (online)	1535-9786
ISSN	1535-9778
DOI	10.1128/EC.00224-14
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Article ; Online: The "finger," a unique multicellular morphology of Candida albicans induced by CO2 and dependent upon the Ras1-cyclic AMP pathway.

Daniels, Karla J / Pujol, Claude / Srikantha, Thyagarajan / Soll, David R

Eukaryotic cell

2012 Volume 11, Issue 10, Page(s) 1257–1267

Abstract: Most experiments exploring the basic biology of pathogenic microbes are performed in vitro under conditions that do not usually mimic those of their host niche. Hence, developmental programs initiated by specific host cues may be missed in vitro. We have ...

Abstract	Most experiments exploring the basic biology of pathogenic microbes are performed in vitro under conditions that do not usually mimic those of their host niche. Hence, developmental programs initiated by specific host cues may be missed in vitro. We have tested the effects of growing low-density agar cultures of the yeast pathogen Candida albicans in concentrations of CO(2) found in the gastrointestinal tract. It is demonstrated that in physiological concentrations of CO(2) at 37°C, yeast cells form a heretofore undescribed multicellular "finger" morphology distinct from a previously described stalk-like structure induced by high doses of UV irradiation that kills more than 99.99% of cells. The finger extends aerially, is uniform in diameter, and is visible to the naked eye, attaining lengths of 3 mm. It is composed of a basal yeast cell monolayer adhering to a semispherical crater formed in the agar and connected to a basal bulb of yeast cells at a fragile interface. The bulb extends into the long shaft. We propose that a single, centrally located hypha extending the length of the shaft forms buds at compartment junctions that serve as the source of the yeast cells in the shaft. A mutational analysis reveals finger formation is dependent upon the pathway Ras1→Cdc35→cyclic AMP (cAMP) (PDE2-\|)→Tpk2→Tec1. Because of the mechanically fragile interface and the compactness of bulb and shaft, we suggest that the finger may function as a multicellular dispersal mechanism produced in host niches containing high levels of CO(2).
MeSH term(s)	Candida albicans/cytology ; Candida albicans/genetics ; Candida albicans/growth & development ; Carbon Dioxide/pharmacology ; Cyclic AMP/metabolism ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Hyphae/cytology ; Hyphae/growth & development ; Hyphae/ultrastructure ; Signal Transduction ; ras Proteins/genetics ; ras Proteins/metabolism
Chemical Substances	Fungal Proteins ; Carbon Dioxide (142M471B3J) ; Cyclic AMP (E0399OZS9N) ; ras Proteins (EC 3.6.5.2)
Language	English
Publishing date	2012-08-24
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2077635-4
ISSN	1535-9786 ; 1535-9778
ISSN (online)	1535-9786
ISSN	1535-9778
DOI	10.1128/EC.00217-12
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Article ; Online: Sex: deviant mating in yeast.

Soll, David R / Pujol, Claude / Srikantha, Thyagarajan

Current biology : CB

2009 Volume 19, Issue 13, Page(s) R509–11

Abstract: Recent comparative genomics and mutational studies of the genes regulating mating and meiosis in fungi provide new insights into not only the variability of the key genes, but also the plasticity of the regulatory circuitry in the evolution of mating ... ...

Abstract	Recent comparative genomics and mutational studies of the genes regulating mating and meiosis in fungi provide new insights into not only the variability of the key genes, but also the plasticity of the regulatory circuitry in the evolution of mating systems.
MeSH term(s)	Biological Evolution ; Candida/genetics ; Candida/physiology ; Genes, Mating Type, Fungal ; Meiosis/genetics ; Phylogeny ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/physiology
Language	English
Publishing date	2009-07-14
Publishing country	England
Document type	Comment ; Journal Article
ZDB-ID	1071731-6
ISSN	1879-0445 ; 0960-9822
ISSN (online)	1879-0445
ISSN	0960-9822
DOI	10.1016/j.cub.2009.05.026
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