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  1. Article: Isolation of 5' regulatory region of

    Mohanan, Manoj Vadakkenchery / Pushpanathan, Anunanthini / Jayanarayanan, Ashwin Narayan / Selvarajan, Dharshini / Ramalingam, Sathishkumar / Govind, Hemaprabha / Chinnaswamy, Appunu

    3 Biotech

    2023  Volume 13, Issue 7, Page(s) 228

    Abstract: Chilling Tolerant Divergence 1 (: Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03650-8. ...

    Abstract Chilling Tolerant Divergence 1 (
    Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03650-8.
    Language English
    Publishing date 2023-06-09
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-023-03650-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Overexpression of Glyoxalase III gene in transgenic sugarcane confers enhanced performance under salinity stress.

    Mohanan, Manoj Vadakkenchery / Pushpanathan, Anunanthini / Padmanabhan, Sarath / Sasikumar, Thelakat / Jayanarayanan, Ashwin Narayan / Selvarajan, Dharshini / Ramalingam, Sathishkumar / Ram, Bakshi / Chinnaswamy, Appunu

    Journal of plant research

    2021  Volume 134, Issue 5, Page(s) 1083–1094

    Abstract: The glyoxalase pathway is a check point to monitor the elevation of methylglyoxal (MG) level in plants and is mediated by glyoxalase I (Gly I) and glyoxalase II (Gly II) enzymes in the presence of glutathione. Recent studies established the presence of ... ...

    Abstract The glyoxalase pathway is a check point to monitor the elevation of methylglyoxal (MG) level in plants and is mediated by glyoxalase I (Gly I) and glyoxalase II (Gly II) enzymes in the presence of glutathione. Recent studies established the presence of unique DJ-1/PfpI domain containing protein named glyoxalase III (Gly III) in prokaryotes, involved in the detoxification of MG into D-lactic acid through a single step process. In the present study, eleven transgenic sugarcane events overexpressing EaGly III were assessed for salinity stress (100 mM and 200 mM NaCl) tolerance. Lipid peroxidation as well as cell membrane injury remained very minimal in all the transgenic events indicating reduced oxidative damage. Transgenic events exhibited significantly higher plant water status, gas exchange parameters, chlorophyll, carotenoid, and proline content, total soluble sugars, SOD and POD activity compared to wild type (WT) under salinity stress. Histological studies by taking the cross section showed a highly stable root system in transgenic events upon exposure to salinity stress. Results of the present study indicate that transgenic sugarcane events overexpressing EaGly III performed well and exhibited improved salinity stress tolerance.
    MeSH term(s) Aldehyde Oxidoreductases/metabolism ; Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plants, Genetically Modified/metabolism ; Saccharum/genetics ; Saccharum/metabolism ; Salinity ; Salt Stress ; Stress, Physiological
    Chemical Substances Plant Proteins ; Aldehyde Oxidoreductases (EC 1.2.-) ; glyoxalase III (EC 1.2.-)
    Language English
    Publishing date 2021-04-22
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2077362-6
    ISSN 1618-0860 ; 0918-9440
    ISSN (online) 1618-0860
    ISSN 0918-9440
    DOI 10.1007/s10265-021-01300-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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  3. Article: Overexpression of Glyoxalase III gene in transgenic sugarcane confers enhanced performance under salinity stress

    Mohanan, Manoj Vadakkenchery / Pushpanathan, Anunanthini / Padmanabhan, Sarath / Sasikumar, Thelakat / Jayanarayanan, Ashwin Narayan / Selvarajan, Dharshini / Ramalingam, Sathishkumar / Ram, Bakshi / Chinnaswamy, Appunu

    Journal of plant research. 2021 Sept., v. 134, no. 5

    2021  

    Abstract: The glyoxalase pathway is a check point to monitor the elevation of methylglyoxal (MG) level in plants and is mediated by glyoxalase I (Gly I) and glyoxalase II (Gly II) enzymes in the presence of glutathione. Recent studies established the presence of ... ...

    Abstract The glyoxalase pathway is a check point to monitor the elevation of methylglyoxal (MG) level in plants and is mediated by glyoxalase I (Gly I) and glyoxalase II (Gly II) enzymes in the presence of glutathione. Recent studies established the presence of unique DJ-1/PfpI domain containing protein named glyoxalase III (Gly III) in prokaryotes, involved in the detoxification of MG into D-lactic acid through a single step process. In the present study, eleven transgenic sugarcane events overexpressing EaGly III were assessed for salinity stress (100 mM and 200 mM NaCl) tolerance. Lipid peroxidation as well as cell membrane injury remained very minimal in all the transgenic events indicating reduced oxidative damage. Transgenic events exhibited significantly higher plant water status, gas exchange parameters, chlorophyll, carotenoid, and proline content, total soluble sugars, SOD and POD activity compared to wild type (WT) under salinity stress. Histological studies by taking the cross section showed a highly stable root system in transgenic events upon exposure to salinity stress. Results of the present study indicate that transgenic sugarcane events overexpressing EaGly III performed well and exhibited improved salinity stress tolerance.
    Keywords carotenoids ; cell membranes ; chlorophyll ; gas exchange ; genes ; genetically modified organisms ; glutathione ; histology ; lactic acid ; lipid peroxidation ; prokaryotic cells ; proline ; research ; root systems ; salt stress ; stress tolerance ; sugarcane
    Language English
    Dates of publication 2021-09
    Size p. 1083-1094.
    Publishing place Springer Singapore
    Document type Article
    ZDB-ID 2077362-6
    ISSN 1618-0860 ; 0918-9440
    ISSN (online) 1618-0860
    ISSN 0918-9440
    DOI 10.1007/s10265-021-01300-9
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Ectopic expression of DJ-1/PfpI domain containing Erianthus arundinaceus Glyoxalase III (EaGly III) enhances drought tolerance in sugarcane.

    Mohanan, Manoj Vadakkenchery / Pushpanathan, Anunanthini / Sasikumar, Sarath Padmanabhan Thelakat / Selvarajan, Dharshini / Jayanarayanan, Ashwin Narayan / R, Arun Kumar / Ramalingam, Sathishkumar / Karuppasamy, Sathyamoorthy Nagaranai / Subbiah, Ramanathan / Ram, Bakshi / Chinnaswamy, Appunu

    Plant cell reports

    2020  Volume 39, Issue 11, Page(s) 1581–1594

    Abstract: Key message: Sugarcane transgenic overexpressing EaGly III from Erianthus arundinaceus showed enhanced water deficit stress tolerance. Methylglyoxal (MG), an α-ketoaldehyde formed from either glycolysis or TCA cycle, is capable of causing total cellular ...

    Abstract Key message: Sugarcane transgenic overexpressing EaGly III from Erianthus arundinaceus showed enhanced water deficit stress tolerance. Methylglyoxal (MG), an α-ketoaldehyde formed from either glycolysis or TCA cycle, is capable of causing total cellular damage via the generation of reactive oxygen species (ROS), advanced glycation end products (AGEs) and nucleic acid degradation. Glyoxalase pathway is a ubiquitous pathway known for detoxification of MG, involving key enzymes glyoxalase I (Gly I) and glyoxalase II (Gly II). Recently, a novel and an additional enzyme in glyoxalase pathway, viz., glyoxalase III (Gly III), has been discovered which possesses DJ-1/PfpI domain recognized for detoxifying MG in a single step process without requirement of any coenzyme. In the present study, a Gly III gene isolated from Erianthus arundinaceus, a wild relative of sugarcane, overexpressed in commercially cultivated sugarcane hybrid Co 86032 was assessed for drought tolerance. Morphometric observations revealed that transgenic sugarcane overexpressing EaGly III acquired drought tolerance trait. Oxidative damage caused by triggering generation of ROS has been determined to be low in transgenic plants as compared to wild type (WT). Transgenics resulted in higher relative water content, chlorophyll content, gas exchange parameters, photosynthetic efficiency, proline content and soluble sugars upon water deficit stress. In addition, higher and stable level of superoxide dismutase and peroxidase activities were observed along with minimal lipid peroxidation during drought stress signifying the tolerance mechanism exhibited by transgenic events. There was no significant structural change observed in the root anatomy of transgenic plants. Altogether, EaGly III gene could be considered as a potential candidate for conferring water deficit stress tolerance for sugarcane and other agricultural crops.
    MeSH term(s) Aldehyde Oxidoreductases/genetics ; Aldehyde Oxidoreductases/metabolism ; Carotenoids/metabolism ; Cell Membrane/genetics ; Chlorophyll/genetics ; Chlorophyll/metabolism ; Dehydration ; Droughts ; Ectopic Gene Expression ; Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Roots/anatomy & histology ; Plant Roots/genetics ; Plants, Genetically Modified ; Proline/metabolism ; Saccharum/genetics ; Saccharum/physiology ; Sugars/metabolism
    Chemical Substances Plant Proteins ; Sugars ; Chlorophyll (1406-65-1) ; Carotenoids (36-88-4) ; Proline (9DLQ4CIU6V) ; Aldehyde Oxidoreductases (EC 1.2.-) ; glyoxalase III (EC 1.2.-)
    Language English
    Publishing date 2020-09-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 8397-5
    ISSN 1432-203X ; 0721-085X ; 0721-7714
    ISSN (online) 1432-203X
    ISSN 0721-085X ; 0721-7714
    DOI 10.1007/s00299-020-02585-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Bonn / Germany

    Z 85/705: Show issues

    Order via subito

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    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Ectopic expression of DJ-1/PfpI domain containing Erianthus arundinaceus Glyoxalase III (EaGly III) enhances drought tolerance in sugarcane

    Mohanan, Manoj Vadakkenchery / Pushpanathan, Anunanthini / Sasikumar, Sarath Padmanabhan Thelakat / Selvarajan, Dharshini / Jayanarayanan, Ashwin Narayan / R., Arun Kumar / Ramalingam, Sathishkumar / Karuppasamy, Sathyamoorthy Nagaranai / Subbiah, Ramanathan / Ram, Bakshi / Chinnaswamy, Appunu

    Plant cell reports. 2020 Nov., v. 39, no. 11

    2020  

    Abstract: KEY MESSAGE: Sugarcane transgenic overexpressing EaGly III from Erianthus arundinaceus showed enhanced water deficit stress tolerance. Methylglyoxal (MG), an α-ketoaldehyde formed from either glycolysis or TCA cycle, is capable of causing total cellular ... ...

    Abstract KEY MESSAGE: Sugarcane transgenic overexpressing EaGly III from Erianthus arundinaceus showed enhanced water deficit stress tolerance. Methylglyoxal (MG), an α-ketoaldehyde formed from either glycolysis or TCA cycle, is capable of causing total cellular damage via the generation of reactive oxygen species (ROS), advanced glycation end products (AGEs) and nucleic acid degradation. Glyoxalase pathway is a ubiquitous pathway known for detoxification of MG, involving key enzymes glyoxalase I (Gly I) and glyoxalase II (Gly II). Recently, a novel and an additional enzyme in glyoxalase pathway, viz., glyoxalase III (Gly III), has been discovered which possesses DJ-1/PfpI domain recognized for detoxifying MG in a single step process without requirement of any coenzyme. In the present study, a Gly III gene isolated from Erianthus arundinaceus, a wild relative of sugarcane, overexpressed in commercially cultivated sugarcane hybrid Co 86032 was assessed for drought tolerance. Morphometric observations revealed that transgenic sugarcane overexpressing EaGly III acquired drought tolerance trait. Oxidative damage caused by triggering generation of ROS has been determined to be low in transgenic plants as compared to wild type (WT). Transgenics resulted in higher relative water content, chlorophyll content, gas exchange parameters, photosynthetic efficiency, proline content and soluble sugars upon water deficit stress. In addition, higher and stable level of superoxide dismutase and peroxidase activities were observed along with minimal lipid peroxidation during drought stress signifying the tolerance mechanism exhibited by transgenic events. There was no significant structural change observed in the root anatomy of transgenic plants. Altogether, EaGly III gene could be considered as a potential candidate for conferring water deficit stress tolerance for sugarcane and other agricultural crops.
    Keywords Saccharum arundinaceum ; chlorophyll ; drought tolerance ; gas exchange ; genes ; glycation ; glycolysis ; hybrids ; lipid peroxidation ; morphometry ; nucleic acids ; peroxidase ; photosynthesis ; proline ; reactive oxygen species ; root anatomy ; stress tolerance ; sugarcane ; superoxide dismutase ; tricarboxylic acid cycle ; water content ; water stress ; wild relatives
    Language English
    Dates of publication 2020-11
    Size p. 1581-1594.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 8397-5
    ISSN 1432-203X ; 0721-085X ; 0721-7714
    ISSN (online) 1432-203X
    ISSN 0721-085X ; 0721-7714
    DOI 10.1007/s00299-020-02585-1
    Database NAL-Catalogue (AGRICOLA)

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    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 85/705: Show issues

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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