LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: Analysis of single-cell sequencing results of an elderly patient with myeloid leukemia reveals high expression of multiple oncogenes in monocytes and hematopoietic stem cells.

    Xu, Xiaoli / Xiong, Minjian / Ye, Haiyan / Qi, Yonglei / Zhao, Ying

    Hematology (Amsterdam, Netherlands)

    2023  Volume 28, Issue 1, Page(s) 2240129

    Abstract: Objective: Older patients with acute myeloid leukemia (AML) face a higher risk of death. This study analyzed the gene sequencing results of an elderly AML patient to provide new ideas for treatment.: Methods: This study performed single-cell RNA ... ...

    Abstract Objective: Older patients with acute myeloid leukemia (AML) face a higher risk of death. This study analyzed the gene sequencing results of an elderly AML patient to provide new ideas for treatment.
    Methods: This study performed single-cell RNA sequencing (scRNA-seq) bioinformatics analysis of blood cells in the peripheral blood and bone marrow of a 64-year-old AML-M5 patient before chemotherapy. The differentially expressed genes (DEGs) were identified, and functional enrichment analyses were performed.
    Results: A total of 7990 and 123 DEGs were identified in monocytes and hematopoietic stem cells (HSCs), respectively. Among the top 40 DEGs analyzed,
    Conclusions: In conclusion, when treating elderly AML patients, it is important to consider their individual characteristics in order to optimize treatment strategies.
    MeSH term(s) Humans ; Aged ; Middle Aged ; Monocytes ; Hematopoietic Stem Cells/metabolism ; Bone Marrow/metabolism ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/metabolism ; Oncogenes
    Language English
    Publishing date 2023-07-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 1341428-8
    ISSN 1607-8454 ; 1024-5332 ; 1024-5340
    ISSN (online) 1607-8454
    ISSN 1024-5332 ; 1024-5340
    DOI 10.1080/16078454.2023.2240129
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4538: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Analysis of Data on Fludarabine, Cyclophosphamide, and Rituximab Chemoimmunotherapy for Chronic Lymphocytic Leukemia Shows High Patient Heterogeneity and the Need for More Consideration of Individualized Treatment.

    Xu, Xiaoli / Zhao, Ying / Ye, Haiyan / Qi, Yonglei / Xu, Wenning / Ling, Yiwen / Yang, Shaojiang

    publication RETRACTED

    Computational and mathematical methods in medicine

    2022  Volume 2022, Page(s) 7451395

    Abstract: Methods: Data from single-cell RNA sequencing (RNA-seq) of CLL patients were obtained from the Gene Expression Omnibus database. The R package was utilized to analyze the data, and the relation of results was predicted via the GeneMANIA website. The ... ...

    Abstract Methods: Data from single-cell RNA sequencing (RNA-seq) of CLL patients were obtained from the Gene Expression Omnibus database. The R package was utilized to analyze the data, and the relation of results was predicted via the GeneMANIA website. The information of 7 samples covered three stages: observation stage, pretreatment by CIT with rituximab, fludarabine, and cyclophosphamide (pre-CIT), and post-CIT. The differentially expressed genes (DEGs) were identified, and functional enrichment analyses were performed. B cell subpopulations and pseudotime trajectories analysis was conducted.
    Results: A total of 70,659 DEGs were identified. Each patient's DEGs presented their own characteristics, with low similarity. Therefore, it is difficult to identify potential hub genes. Similarly, pathway enrichment analysis showed significant tumor heterogeneity among CLL patients. Analysis of relapsed post-CIT compared to the observation stage suggested that the
    Conclusions: Tumor heterogeneity may be a more common manifestation of CLL. Individualized treatment should be considered for CLL.
    MeSH term(s) Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Cyclophosphamide/therapeutic use ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/drug therapy ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics ; Rituximab/therapeutic use ; Vidarabine/analogs & derivatives
    Chemical Substances Rituximab (4F4X42SYQ6) ; Cyclophosphamide (8N3DW7272P) ; Vidarabine (FA2DM6879K) ; fludarabine (P2K93U8740)
    Language English
    Publishing date 2022-10-03
    Publishing country United States
    Document type Journal Article ; Retracted Publication
    ZDB-ID 2252430-7
    ISSN 1748-6718 ; 1748-670X ; 1027-3662
    ISSN (online) 1748-6718
    ISSN 1748-670X ; 1027-3662
    DOI 10.1155/2022/7451395
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6373: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: circZNF91 Promotes the Malignant Phenotype of Chronic Lymphocytic Leukemia Cells by Targeting the miR-1283/WEE1 Axis.

    Li, Shaoting / Chen, Jing / Fan, Ying / Xu, Xiaoli / Xiong, Minjian / Qi, Yonglei / Wu, Wenlin / Zhao, Ying

    BioMed research international

    2022  Volume 2022, Page(s) 2855394

    Abstract: Background: Circular RNAs (circRNAs) are frequently dysregulated in cancers and are implicated in tumorigenesis and tumor progression. In this study, we investigated the role of circZNF91 in regulating the malignant phenotype of chronic lymphocytic ... ...

    Abstract Background: Circular RNAs (circRNAs) are frequently dysregulated in cancers and are implicated in tumorigenesis and tumor progression. In this study, we investigated the role of circZNF91 in regulating the malignant phenotype of chronic lymphocytic leukemia (CLL) cells and the underlying molecular mechanism.
    Material: /.
    Methods: The expression of circZNF91 was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The binding sequences between circZNF91/miR-1283 and miR-1283/WEE1 were predicted by the bioinformatic database. The functional interactions were confirmed by the dual-luciferase reporter, RT-qPCR, and Western blot assays. The functional roles of the circZNF91/miR-1283/WEE1 axis in CLL progression were examined by cell proliferation, apoptosis, and EdU incorporation assays.
    Results: circZNF91 was upregulated in CLL samples. Silencing circZNF91 attenuated CLL cell proliferation and induced apoptosis and cell cycle arrest. circZNF91 could sponge miR-1283 to suppress its activity, which in turn upregulated WEE1 expression. Silencing circ-TTBK2 reduced WEE1 expression, while the inhibitor of miR-1283 enhanced WEE1 expression. The miR-1283/WEE1 axis mediated the effects of circZNF91 on cell proliferation and apoptosis, as well as induced cell cycle regulation.
    Conclusions: The circZNF91/miR-1283/WEE1 axis is engaged in the pathological phenotypes of CLL cells, which could serve as potential targets for future therapy development.
    MeSH term(s) Apoptosis/genetics ; Cell Cycle Proteins/genetics ; Cell Line, Tumor ; Cell Proliferation/genetics ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Phenotype ; Protein-Tyrosine Kinases/metabolism ; RNA, Circular/genetics
    Chemical Substances Cell Cycle Proteins ; MIRN1283 microRNA, human ; MicroRNAs ; RNA, Circular ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; WEE1 protein, human (EC 2.7.10.2)
    Language English
    Publishing date 2022-05-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2022/2855394
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: [Efficacy of Oral Cyclophosphamide for Treatment of Refractory T-cell Large Granular Lymphocytic Leukemia Accompanied with Pure Erythrocyte Aplastic Anemia].

    Zhao, Ying / Chen, Zhuo-Wen / Ye, Hai-Yan / Gu, Jian / Xu, Xiao-Li / Qi, Yong-Lei / Ling, Yi-Wen

    Zhongguo shi yan xue ye xue za zhi

    2020  Volume 28, Issue 2, Page(s) 418–423

    Abstract: Objective: To explore the clinical effects of oral small dose of cyclophosphamide (CTX) in the treatment of T-cell large granular lymphocytic leukemia (T-LGLL) accompanied with pure red cell aplasia (PRCA).: Methods: The clinical features, ... ...

    Abstract Objective: To explore the clinical effects of oral small dose of cyclophosphamide (CTX) in the treatment of T-cell large granular lymphocytic leukemia (T-LGLL) accompanied with pure red cell aplasia (PRCA).
    Methods: The clinical features, characteristics of laboratory examinations and the process of oral small dose of CTX treatment after the ineffective treatment of cyclosporine A combining with prednisone in 1 case of T-LGLL with PRCA were reported and discussed with related references.
    Results: The elderly female patient had indolent process, mainly presenting with anemia and significant low hyperplasia of bone marrow erythrocyte cells. Peripheral blood smear showed mainly with characteristic large granular lymphocytic morphology. The results of immunophenotypic analyses and genetic reassortment were compatible with T-LGLL. No effects were shown after 5 months of cyclosporine A combining with prednisone treatment and the patient still needed recurrent blood transfusion. CTX was prescribed as a second-line medication and the dose was 100 mg/d. Hemoglobin could returned to normal level and the efficacy remained for 1 year even after the medication was stopped.
    Conclusion: T-LGLL accompanied with PRCA is a rare disease and oral small dose CTX can be an effective therapeutic regimen.
    MeSH term(s) Aged ; Anemia, Aplastic/complications ; Cyclophosphamide ; Erythrocytes ; Female ; Humans ; Leukemia, Large Granular Lymphocytic/complications
    Chemical Substances Cyclophosphamide (8N3DW7272P)
    Language Chinese
    Publishing date 2020-04-21
    Publishing country China
    Document type Journal Article
    ZDB-ID 2404306-0
    ISSN 1009-2137
    ISSN 1009-2137
    DOI 10.19746/j.cnki.issn.1009-2137.2020.02.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6603: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top