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  1. Article ; Online: Research progress on the effects of gut microbiome on lung damage induced by particulate matter exposure.

    Qin, Jiali / Wang, Junling

    Environmental research

    2023  Volume 233, Page(s) 116162

    Abstract: Air pollution is one of the top five causes of death in the world and has become a research hotspot. In the past, the health effects of particulate matter (PM), the main component of air pollutants, were mainly focused on the respiratory and ... ...

    Abstract Air pollution is one of the top five causes of death in the world and has become a research hotspot. In the past, the health effects of particulate matter (PM), the main component of air pollutants, were mainly focused on the respiratory and cardiovascular systems. However, in recent years, the intestinal damage caused by PM and its relationship with gut microbiome (GM) homeostasis, thereby affecting the composition and function of GM and bringing disease burden to the host lung through different mechanisms, have attracted more and more attention. Therefore, this paper reviews the latest research progress in the effect of PM on GM-induced lung damage and its possible interaction pathways and explores the potential immune inflammatory mechanism with the gut-lung axis as the hub in order to understand the current research situation and existing problems, and to provide new ideas for further research on the relationship between PM pollution, GM, and lung damage.
    MeSH term(s) Particulate Matter/toxicity ; Gastrointestinal Microbiome ; Lung ; Air Pollutants/analysis ; Air Pollution/adverse effects ; Air Pollution/analysis
    Chemical Substances Particulate Matter ; Air Pollutants
    Language English
    Publishing date 2023-06-20
    Publishing country Netherlands
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 205699-9
    ISSN 1096-0953 ; 0013-9351
    ISSN (online) 1096-0953
    ISSN 0013-9351
    DOI 10.1016/j.envres.2023.116162
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Inhibition of canine parvovirus 2 (CPV-2) replication by TAT-scFv through targeting of the viral structural protein VP2 of CPV-2.

    Liu, Kun / Xu, Peng / Li, Yuchen / Qin, Jiali / Zhu, Jiping / Li, Yi

    The new microbiologica

    2023  Volume 46, Issue 4, Page(s) 381–389

    Abstract: Canine parvovirus (CPV) causes severe infectious disease with a high mortality rate in dogs. CPV is still a major health issue of dogs in the clinic. Therefore, there is an urgent need to develop effective drugs to treat the disease. In this study, we ... ...

    Abstract Canine parvovirus (CPV) causes severe infectious disease with a high mortality rate in dogs. CPV is still a major health issue of dogs in the clinic. Therefore, there is an urgent need to develop effective drugs to treat the disease. In this study, we fused the transactivating transcriptional activator peptide (TAT) with scFv. TAT-scFv was identified by Western blot. CCK8 kit was used to detect the toxicity of TAT-scFv to cells. The binding activity of TAT-scFv to CPV-2-VP2 was detected by DAS ELISA. The cell uptake rate of TAT-scFv was assessed by IFA. After infection with CPV-2, F81 cells were incubated by TAT-scFv. The replication of virus was measured to determine the neutralization effect of TAT-scFv on intracellular and extracellular viruses. Protein docking was used to predict the amino acid (AA) sites of VP2 binding to TAT-scFv. TAT-scFv was expressed in Escherichia coli and purified. The DAS ELISA showed that TAT-scFv could bind with CPV-2-VP2. We demonstrated that TAT-scFv entered cells in a dose-dependent and time-dependent manner and effectively inhibited the replication of CPV-2. Using protein docking, we determined the interaction pattern and found that the N-terminal region (AA 41-49) and the C-terminal region (AA 558) of VP2 interacted with the TAT-scFv. Taken together, these results suggest that, TAT-scFv may be a potential antiviral drug for inhibiting CPV-2 replication and controlling disease caused by CPV-2.
    MeSH term(s) Animals ; Dogs ; Parvovirus, Canine ; Peptides ; Antiviral Agents/pharmacology ; Escherichia coli/genetics
    Chemical Substances Peptides ; Antiviral Agents
    Language English
    Publishing date 2023-03-08
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 756168-4
    ISSN 1121-7138 ; 0391-5352
    ISSN 1121-7138 ; 0391-5352
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Motility-related microRNAs identified in pig seminal plasma exosomes by high-throughput small RNA sequencing.

    Zhao, Yunxiang / Qin, Jiali / Sun, Jingshuai / He, Jian / Sun, Yanmei / Yuan, Renqiang / Li, Zhili

    Theriogenology

    2023  Volume 215, Page(s) 351–360

    Abstract: Boar fertility is a key determinant of the production efficiency of the whole pig breeding industry and boar sperm motility is the seminal parameter with the greatest impact on the fecundity of a sow. Exosomes are small, extracellular vesicles found in ... ...

    Abstract Boar fertility is a key determinant of the production efficiency of the whole pig breeding industry and boar sperm motility is the seminal parameter with the greatest impact on the fecundity of a sow. Exosomes are small, extracellular vesicles found in many body fluids. Seminal plasma exosomes, which are secreted by the epididymis, prostate, seminal vesicles, and testes, contain a large number of miRNAs, the types and levels of which can reflect the physiological state of source cells. It has been shown that the expression profile of seminal plasma exosomal miRNA differs between low-motility semen and normal semen. The aim of this study was to investigate the relationship between semen motility and exosomal miRNA profiles to obtain information that would allow to predict boar fertility, as well as contribute to the understanding of the mechanisms by which exosomal miRNAs regulate semen motility. Three high-motility (semen motility >90 %) and three low-motility (semen motility <80 %) semen samples were collected from Landrace and Yorkshire boars, respectively, and seminal plasma exosomes were extracted by ultracentrifugation. Exosome characterization was performed using transmission electron microscopy, NTA, and Western blot. The expression profiles of exosomal miRNAs associated with semen motility in the two boar breeds were subsequently determined by small RNA sequencing. The results showed that 297 known miRNAs and 295 novel RNAs were co-expressed in the four groups. Notably, six miRNAs (ssc-miR-122-5p, ssc-miR-486, ssc-miR-451, ssc-miR-345-3p, ssc-miR-362, and ssc-miR-500-5p) were found to be differentially expressed in both boar breeds. Enrichment analysis of the target genes of the differentially expressed miRNAs showed that they were mainly involved in biological processes such as regulation of transcription from RNA polymerase II promoter, regulation of gene expression, and intracellular signal transduction and signaling pathways such as the PI3K-Akt, MAPK, and Ras signaling pathways. The six differentially expressed miRNAs identified in this study have significant potential as noninvasive markers of boar semen motility. Meanwhile, the results of the enrichment analysis provide novel insights into the mechanisms underlying the regulation of semen motility.
    MeSH term(s) Swine ; Male ; Animals ; Female ; Semen/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Exosomes/genetics ; Exosomes/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Sperm Motility ; Sequence Analysis, RNA/veterinary
    Chemical Substances MicroRNAs ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-)
    Language English
    Publishing date 2023-12-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 189232-0
    ISSN 1879-3231 ; 0093-691X
    ISSN (online) 1879-3231
    ISSN 0093-691X
    DOI 10.1016/j.theriogenology.2023.11.028
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Effects of intravenous nicardipine followed by oral labetalol in combination with nifedipine controlled-release tablet on severe peripartum hypertension.

    Lan, Lan / Yan, Yunsheng / Qi, Haifeng / Qin, Jiali / Li, Lan / Gan, Shengwen / Zhang, Ruoxuan / Zhang, Yaozong

    Ginekologia polska

    2024  

    Abstract: Objectives: To investigate the effects of intravenous nicardipine as initial therapy and oral labetalol combined with nifedipine controlled-release tablet as subsequent treatment of severe peripartum hypertension.: Material and methods: Intravenous ... ...

    Abstract Objectives: To investigate the effects of intravenous nicardipine as initial therapy and oral labetalol combined with nifedipine controlled-release tablet as subsequent treatment of severe peripartum hypertension.
    Material and methods: Intravenous nicardipine was delivered as the initial treatment, after the target blood pressure (BP) had been achieved, oral labetalol was used to maintain the target BP. If oral labetalol failed to maintain the target BP, oral labetalol combined with nifedipine controlled-release tablet was used.
    Results: A total number of 131 patients were enrolled. The target BP (BP < 140/90 mmHg) was achieved in all patients within 60 minutes by intravenous nicardipine. After receiving labetalol orally, the target BP was maintained in nine patients. However, in 104 patients, we had to combine oral labetalol and nifedipine controlled-release tablet due to re-elevation of their systolic BP to 140-159 mmHg. In 18 patients, we restarted intravenous nicardipine because their systolic BP re-elevated above 160 mm Hg. Among the 104 patients who received oral labetalol and nifedipine controlled-release tablet, the target BP was achieved and maintained in 96 patients, and eight patients had to restart nicardipine. Of the total number of 26 patients in whom intravenous nicardipine was resumed, the target BP was successfully maintained in 22 patients with oral labetalol combined with nifedipine controlled-release tablet.
    Conclusions: Intravenous nicardipine rapidly and safely lowered severe peripartum hypertension. As subsequent therapy, oral labetalol combined with nifedipine controlled-release tablet protocol may be applied to effectively maintain a target BP.
    Language English
    Publishing date 2024-02-09
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 130894-4
    ISSN 2543-6767 ; 0017-0011
    ISSN (online) 2543-6767
    ISSN 0017-0011
    DOI 10.5603/gpl.97752
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Metabolomic analysis reveals spermatozoa and seminal plasma differences between Duroc and Liang guang Small-spotted pig.

    Li, Zhili / Sun, Jingshuai / Li, Kebiao / Qin, Jiali / Sun, Yanmei / Zeng, Jianhua / El-Ashram, Saeed / Zhao, Yunxiang

    Frontiers in veterinary science

    2023  Volume 9, Page(s) 1078928

    Abstract: The Liang guang Small-spotted pig is a well-known Chinese indigenous pig that is valued for its exceptional meat quality. However, the Liang guang Small-spotted pig has a lower semen storage capacity, shorter storage time and worse semen quality compared ...

    Abstract The Liang guang Small-spotted pig is a well-known Chinese indigenous pig that is valued for its exceptional meat quality. However, the Liang guang Small-spotted pig has a lower semen storage capacity, shorter storage time and worse semen quality compared to Duroc. Pig sperm used for artificial insemination (AI) loses part of vitality and quality when being stored in commercial solutions. Serious vitality losses and short shelf life of the semen are particularly prominent in Liang guang Small-spotted pig. In this study, the metabolites in seminal plasma and spermatozoa of Duroc and Liang guang Small-spotted pigs were identified using UHPLC-Q-TOF/MS technology. The findings indicated forty distinct metabolites concentrating on energy metabolic substrates and antioxidant capacity in Liang guang Small-spotted pig and Duroc seminal plasma, including D-Fructose, succinate, 2-dehydro-3-deoxy-d-gluconate, alanine betaine, citrate, carnitine, acetylcarnitine and so on. Seventeen different metabolites were explored, with a focus on glycerophospholipid metabolism in Liang guang Small-spotted pig and Duroc spermatozoa, primarily including glycerol 3-phosphate, acetylcarnitine, phosphatidylcholine (PC) 16:0/16:0, palmitoyl sphingomyelin, acetylcholine, choline, glycerophosphocholine, betaine, L-carnitine, creatinine and others. This study reveals the metabolite profile of spermatozoa and seminal plasma among different pig breeds and might be valuable for understanding the mechanisms that lead to sperm storage capacity. Metabolites involved in energy metabolism, antioxidant capacity and glycerophospholipid metabolism might be key to the poor sperm storage capacity in Liang guang Small-spotted pig.
    Language English
    Publishing date 2023-01-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2022.1078928
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Nine‐year organic fertilization enhances poorly crystalline iron hydroxide formation and phosphorus availability in a temperate rice–wheat cropping system

    Qin, Jiali / Han, Xinci / Jia, Zhixin / Li, Yunyan / Zhang, Dandan / Li, Lina / Xie, Junyu / Li, Li / Li, Tingliang / Huang, Xiaolei / Ling, Ning / Yu, Guanghui

    Soil Science Society of America Journal. 2023 , v. 87, no. 4 p.808-820

    2023  

    Abstract: Phosphorus (P) is typically associated with iron (Fe) hydroxides in paddy soils. Our study investigated the impact of different fertilization treatments on the availability of P regulated by Fe redox transformation in a rice (Oryza sativa L.)–wheat ( ... ...

    Abstract Phosphorus (P) is typically associated with iron (Fe) hydroxides in paddy soils. Our study investigated the impact of different fertilization treatments on the availability of P regulated by Fe redox transformation in a rice (Oryza sativa L.)–wheat (Triticum aestivum L.) cropping system in the mid‐low reaches of the Yangtze River. Five fertilization treatments were examined: control (CK), chemical fertilizer (CF), 35% CF (P) plus pig manure compost (CFM), 100% CF (P) plus straw (CFS), and 35% CF (P) plus pig manure compost and straw (CFMS). Nine‐year rice–wheat rotations increased the oxalate‐extractable poorly crystalline Fe hydroxides (i.e., Feo) by 33%–87% compared with the initial soil, while fertilization further accelerated this process. Compost application increased the proportion of labile P by 75%–108% and decreased the proportion of non‐labile P by 14%–22% compared with the single chemical fertilization treatment. Furthermore, organic fertilization increased the mass proportions of macroaggregates and macroaggregate‐associated labile P. The Feo was positively correlated with the content of labile P but negatively correlated with the proportion of non‐labile P. Moreover, the reductive dissolution of Fe hydroxides was accompanied by the transformation of P from NaOH‐extractable to NaHCO₃‐ and H₂O‐extractable phases. These results indicate that seasonal alternation of drying and wetting can progressively drive the redox transformation of Fe hydroxides and promote the formation of Feo, thereby affecting the availability of P. Therefore, we suggested that P fertilizer should be reduced in the rice season due to the reduction of Fe hydroxides, particularly in the compost‐amended soils in the temperate rice–wheat cropping system.
    Keywords Oryza sativa ; Triticum aestivum ; composted manure ; iron ; paddies ; phosphorus ; phosphorus fertilizers ; pig manure ; redox reactions ; rice ; soil ; straw ; Yangtze River
    Language English
    Dates of publication 2023-07
    Size p. 808-820.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 196788-5
    ISSN 0361-5995
    ISSN 0361-5995
    DOI 10.1002/saj2.20548
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: [Comparison of the diagnostic value of high frequency ultrasound and ultrasound-guided fine needle aspiration biopsy in papillary thyroid microcarcinoma].

    Qin, Jiali / Zhang, Shasha / Li, Gang / Liu, Shanting / Shi, Xiangyu / Wu, Junfu

    Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery

    2021  Volume 35, Issue 8, Page(s) 718–723

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Biopsy, Fine-Needle ; Carcinoma, Papillary ; Humans ; Retrospective Studies ; Sensitivity and Specificity ; Thyroid Neoplasms/diagnostic imaging ; Thyroid Nodule ; Ultrasonography, Interventional
    Language Chinese
    Publishing date 2021-07-26
    Publishing country China
    Document type Journal Article
    ISSN 2096-7993
    ISSN 2096-7993
    DOI 10.13201/j.issn.2096-7993.2021.08.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: DNA double-strand break repair gene mutation and the risk of papillary thyroid microcarcinoma: a case-control study.

    Qin, Jiali / Fan, Jie / Li, Gang / Liu, Shanting / Liu, Zhensheng / Wu, Yao

    Cancer cell international

    2021  Volume 21, Issue 1, Page(s) 334

    Abstract: Objective: To study the relationship between DNA double-strand break (DSB) repair gene mutations and the risk of papillary thyroid microcarcinoma (PTMC).: Methods: One hundred patients with PTMC or benign thyroid nodules (BTNs) at Henan Cancer ... ...

    Abstract Objective: To study the relationship between DNA double-strand break (DSB) repair gene mutations and the risk of papillary thyroid microcarcinoma (PTMC).
    Methods: One hundred patients with PTMC or benign thyroid nodules (BTNs) at Henan Cancer Hospital were retrospectively analyzed. The DSB repair capacity of peripheral blood T lymphocytes in the two groups was assessed by flow cytometry. Data were compared using Student's t-test to evaluate the relationship between DSB repair capacity and the risk of PTMC. Factors influencing DSB repair capacity were analyzed by multivariate logistic regression analysis. The relationship between PTMC and DSB repair capacity was analyzed by univariate analysis. Targeted next-generation DNA sequencing was applied to screen and analyze DSB repair genes related to PTMC.
    Results: The DSB repair capacity was 31.30% in the PTMC group and 44.40% in the BTN group, with that of the former being significantly lower (P < 0.05). Multivariate logistic regression analysis of age, sex, obesity status, radiation and other factors showed that radiation exposure was positively correlated with reduced DSB repair capacity(OR = 3.642; 95% CI 1.484-8.935, P = 0.020). Moreover, univariate analysis showed that a reduction in DSB repair capacity was a risk factor for PTMC(OR = 2.333; 95% CI 1.027-5.300, P = 0.043).Targeted next-generation DNA sequencing was performed on the DSB repair genes discovered, and those that were mutated in association with PTMC were Rad50 and FANCA; Rad51 mutations were related to BTN.
    Conclusion: Radiation exposure is positively associated with induced DSB repair gene mutations, which may cause a reduced capacity for DSB repair and eventually lead to PTMC.
    Language English
    Publishing date 2021-07-02
    Publishing country England
    Document type Journal Article
    ISSN 1475-2867
    ISSN 1475-2867
    DOI 10.1186/s12935-021-02032-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: A risk score for early predicting bloodstream infections in febrile obstetric patients: a pilot study.

    Zhang, Yaozong / Li, Lan / Yan, Yunsheng / Qi, Haifeng / Qin, Jiali / Ren, Li / Zhang, Ruoxuan

    Archives of gynecology and obstetrics

    2021  Volume 306, Issue 1, Page(s) 85–92

    Abstract: Purpose: Early prediction of bloodstream infections (BSI) among obstetric patients remains to be a challenge for clinicians. The objective of this study was to develop a risk score and assess its discriminative ability in febrile obstetric patients in a ...

    Abstract Purpose: Early prediction of bloodstream infections (BSI) among obstetric patients remains to be a challenge for clinicians. The objective of this study was to develop a risk score and assess its discriminative ability in febrile obstetric patients in a maternal intensive care unit (ICU).
    Methods: Between May 2015 and August 2020, a total of 497 febrile obstetric patients were categorized into BSI group (n = 276) and Non-BSI group (n = 221) based on the result of blood cultures. White blood cell count, C-reactive protein (CRP), procalcitonin (PCT), time of interval from amniorrhea to fever (IFAF) and maximum body temperature (T
    Results: The levels of neutrophil, CRP, PCT, IFAF and T
    Conclusion: The risk score has a feasible discriminatory ability in early prediction of BSI in febrile obstetric patients.
    MeSH term(s) C-Reactive Protein/analysis ; Female ; Fever/diagnosis ; Fever/etiology ; Humans ; Pilot Projects ; Pregnancy ; Procalcitonin ; ROC Curve ; Retrospective Studies ; Risk Factors ; Sepsis/diagnosis
    Chemical Substances Procalcitonin ; C-Reactive Protein (9007-41-4)
    Language English
    Publishing date 2021-10-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 896455-5
    ISSN 1432-0711 ; 0932-0067
    ISSN (online) 1432-0711
    ISSN 0932-0067
    DOI 10.1007/s00404-021-06269-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Li2ZnTi3O8/C anode with high initial Coulombic efficiency, long cyclic life and outstanding rate properties enabled by fulvic acid

    Qin, Jia-Li / Zhu, Hui-Ling / Lun, Ning / Qi, Yong-Xin / Bai, Yu-Jun

    Carbon. 2020 Aug. 15, v. 163

    2020  

    Abstract: Industrialized anode materials for Li-ion batteries (LIBs) require high initial Coulombic efficiency (ICE), long cyclic life and outstanding rate properties. Herein, fulvic acid (FA) with the elements of C, H, O, N and S was adopted as the carbon ... ...

    Abstract Industrialized anode materials for Li-ion batteries (LIBs) require high initial Coulombic efficiency (ICE), long cyclic life and outstanding rate properties. Herein, fulvic acid (FA) with the elements of C, H, O, N and S was adopted as the carbon precursor to mix with Li2ZnTi3O8 (LZTO) followed by carbonizing to optimize the electrochemical performance of the LZTO anode. At a weight ratio of 0.28 for FA/LZTO, the product carbonized at 800 °C reveals high ICE of 88.0%, remarkable rate capabilities (lithiation/delithiation capacities of 195.4/191 and 143.5/143.5 mAh g−1 at 0.1 and 1.6 A g−1, respectively). After undergoing 1000 cycles at 0.5 A g−1, the retained reversible capacity is 215.3 mAh g−1. The excellent performance suitable for industrial power LIBs associates with the N and S co-doped discontinuous carbon coating layers, small carbon bumps and membranes on the LZTO particle surface. The carbon material facilitates electron transfer and the exposed LZTO surface maintains the good Li-ion migration of LZTO. Particularly, the NO3− radicals yielded favor to boost the Li-ion diffusion by transforming into the solid state electrolytes of Li3N and LiNxOy in the lithiation process.
    Keywords anodes ; carbon ; coatings ; electrochemistry ; electrolytes ; electron transfer ; free radicals ; fulvic acids ; industrialization ; lithium ; lithium batteries ; nitrates
    Language English
    Dates of publication 2020-0815
    Size p. 297-307.
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 0008-6223
    DOI 10.1016/j.carbon.2020.03.029
    Database NAL-Catalogue (AGRICOLA)

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