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  1. Article ; Online: Portable Detection of Lysine Acetyltransferase Activity in Lung Cancer Cells Based on a Miniature Electrochemical Sensor.

    Liu, Jiayue / Lu, Yanmei / Hu, Yufang / Zhang, Qingqing / Wang, Sui / Guo, Zhiyong / Qing, Zhihe

    Analytical chemistry

    2024  Volume 96, Issue 14, Page(s) 5546–5553

    Abstract: The detection of lysine acetyltransferases is crucial for diagnosing and treating lung cancer, highlighting the necessity for highly efficient detection methods. We developed a portable, highly accurate, and sensitive technique using fast-scan cyclic ... ...

    Abstract The detection of lysine acetyltransferases is crucial for diagnosing and treating lung cancer, highlighting the necessity for highly efficient detection methods. We developed a portable, highly accurate, and sensitive technique using fast-scan cyclic voltammetry (FSCV) to determine the activity of the lysine acetyltransferase TIP60, employing a novel miniature electrochemical sensor. This approach involves a compact electrochemical cell, merely 3 mm in diameter, that holds solutions up to 50 μL, equipped with a conductive indium tin oxide working electrode. Uniquely, this system operates on a two-electrode model compatible with the FSCV, obviating the traditional requirement for a reference electrode. The system detects TIP60 activity through the continuous generation of CoA molecules that engage in reactions with Cu(II), thereby significantly improving the accuracy of the acetylation analysis. Remarkably, the detection limit achieved for TIP60 is notably low at 3.3 pg/mL (
    MeSH term(s) Humans ; Lung Neoplasms/diagnosis ; Lysine Acetyltransferases ; Electrochemical Techniques
    Chemical Substances Lysine Acetyltransferases (EC 2.3.1.32)
    Language English
    Publishing date 2024-03-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.3c05908
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
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  2. Article ; Online: Pt-Decorated Gold Nanoflares for High-Fidelity Phototheranostics: Reducing Side-Effects and Enhancing Cytotoxicity toward Target Cells.

    Quan, Ke / Li, Xiaoyuan / Deng, Jiaqi / Chen, Weiju / Zou, Zhen / Chen, Kun / Wu, Linlin / Liu, Juewen / Qing, Zhihe

    Angewandte Chemie (International ed. in English)

    2024  , Page(s) e202402881

    Abstract: Functionalized with the Au-S bond, gold nanoflares have emerged as promising candidates for theranostics. However, the presence of intracellular abundantly biothiols compromises the conventional Au-S bond, leading to the unintended release of cargoes and ...

    Abstract Functionalized with the Au-S bond, gold nanoflares have emerged as promising candidates for theranostics. However, the presence of intracellular abundantly biothiols compromises the conventional Au-S bond, leading to the unintended release of cargoes and associated side-effects on non-target cells. Additionally, the hypoxic microenvironment in diseased regions limits treatment efficacy, especially in photodynamic therapy. To address these challenges, high-fidelity photodynamic nanoflares constructed on Pt-coated gold nanoparticles (Au@Pt PDNF) were communicated to avoid false-positive therapeutic signals and side-effects caused by biothiol perturbation. Compared with conventional photodynamic gold nanoflares (AuNP PDNF), the Au@Pt PDNF were selectively activated by cancer biomarkers and exhibited high-fidelity phototheranostics while reducing side-effects. Furthermore, the ultrathin Pt-shell catalysis was confirmed to generate oxygen which alleviated hypoxia-related photodynamic resistance and enhanced the antitumor effect. This design might open a new venue to advance theranostics performance and is adaptable to other theranostic nanomaterials by simply adding a Pt shell.
    Language English
    Publishing date 2024-03-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202402881
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  3. Article ; Online: Amplified Biosensors Powered by Endogenous Molecules for Intracellular Fluorescence Imaging.

    Zhou, Yibo / Zhang, Jun / Sun, Shuanghong / Chen, Weiju / Wang, Yuping / Shi, Huiqiu / Yang, Ronghua / Qing, Zhihe

    Analytical chemistry

    2024  

    Language English
    Publishing date 2024-04-15
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.4c00329
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  4. Article ; Online: Lowering Entropic Barriers in Triplex DNA Switches Facilitating Biomedical Applications at Physiological pH.

    Lei, Yanli / Li, Chuangchuang / Ji, Xinyue / Sun, Haiyan / Liu, Xiaowen / Mao, Zenghui / Chen, Weiju / Qing, Zhihe / Liu, Juewen

    Angewandte Chemie (International ed. in English)

    2024  Volume 63, Issue 19, Page(s) e202402123

    Abstract: Triplex DNA switches are attractive allosteric tools for engineering smart nanodevices, but their poor triplex-forming capacity at physiological conditions limited the practical applications. To address this challenge, we proposed a low-entropy barrier ... ...

    Abstract Triplex DNA switches are attractive allosteric tools for engineering smart nanodevices, but their poor triplex-forming capacity at physiological conditions limited the practical applications. To address this challenge, we proposed a low-entropy barrier design to facilitate triplex formation by introducing a hairpin duplex linker into the triplex motif, and the resulting triplex switch was termed as CTNS
    MeSH term(s) Hydrogen-Ion Concentration ; DNA/chemistry ; Humans ; Entropy ; Nucleic Acid Conformation ; Biosensing Techniques
    Chemical Substances DNA (9007-49-2) ; triplex DNA
    Language English
    Publishing date 2024-04-03
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202402123
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  5. Article ; Online: One-step, reagent-free construction of nano-enzyme as visual and reusable biosensor for oxidase substrates.

    Quan, Ke / Zeng, Yuqing / Zhang, Wenke / Li, Fengfeng / Li, Mengjiao / Qing, Zhihe / Wu, Linlin

    Analytica chimica acta

    2023  Volume 1285, Page(s) 342008

    Abstract: The substrates of oxidase are biologically essential substances that are closely associated with human physiological health. However, current biosensing methods suffer from tough recyclability and undesired denaturation of enzyme due to impurity ... ...

    Abstract The substrates of oxidase are biologically essential substances that are closely associated with human physiological health. However, current biosensing methods suffer from tough recyclability and undesired denaturation of enzyme due to impurity interference. Herein, we have developed a visual and reusable biosensor for detecting substrate using glucose oxidase (GOx) as a model oxidase. GOx was immobilized onto gold nanoparticles (AuNPs) at -20 °C in one step without additional reagents. The resulting nano-enzyme generated coloimetric signals by coupling with horseradish peroxidase (HRP) using TMB as the substrate. Our results demonstrated that the immobilized GOx exhibited satisfactory sensitivity (0.68 μM) for glucose detection and higher inherent stability than free GOx under harsh conditions, enabling reliable detection of glucose in complex fluids (colored beverages and saliva). Furthermore, the nano-enzyme retained 80 % activity even after four cycles of catalytic oxidation. This strategy constructs a universal biosensor for substrates with nano-enzyme which rely only on intrinsic cysteine within the oxidase while avoiding functional handle modification.
    MeSH term(s) Humans ; Oxidoreductases ; Enzymes, Immobilized/chemistry ; Gold ; Indicators and Reagents ; Metal Nanoparticles ; Glucose ; Glucose Oxidase/chemistry ; Biosensing Techniques/methods
    Chemical Substances Oxidoreductases (EC 1.-) ; Enzymes, Immobilized ; Gold (7440-57-5) ; Indicators and Reagents ; Glucose (IY9XDZ35W2) ; Glucose Oxidase (EC 1.1.3.4)
    Language English
    Publishing date 2023-11-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2023.342008
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  6. Article ; Online: A Reversible Fluorescent Probe for In Situ Monitoring Redox Imbalance during Mitophagy

    Li, Junbin / Song, Lifei / Hu, Weiguo / Zuo, Qin / Li, Roumei / Dai, Min / Zhou, Yibo / Qing, Zhihe

    Analytical Chemistry. 2023 Aug. 29, v. 95, no. 36 p.13668-13673

    2023  

    Abstract: Mitophagy is the lysosome-dependent degradation of damaged and dysfunctional mitochondria, which is closely associated with H₂O₂-related redox imbalance and pathological processes. However, development of fast-responding and highly sensitive reversible ... ...

    Abstract Mitophagy is the lysosome-dependent degradation of damaged and dysfunctional mitochondria, which is closely associated with H₂O₂-related redox imbalance and pathological processes. However, development of fast-responding and highly sensitive reversible fluorescent probes for monitoring of mitochondrial H₂O₂ dynamics is still lacking. Herein, we report a reversible fluorescent probe (M-HP) that enables real-time imaging of H₂O₂-related redox imbalance. In vitro studies demonstrated that M-HP had a rapid response and high sensitivity to the H₂O₂/GSH redox cycle, with a detection limit of 17 nM for H₂O₂. M-HP was applied to imaging of H₂O₂ fluctuation in living cells with excellent reversibility and mitochondrial targeting. M-HP reveals an increase in mitochondrial H₂O₂ under lipopolysaccharide stimulation and a decrease in H₂O₂ following the combined treatment with rapamycin. This suggests that the level of oxidative stress is significantly suppressed after the enhancement of mitophagy. The rationally designed M-HP offers a powerful tool for understanding redox imbalance during mitophagy.
    Keywords analytical chemistry ; detection limit ; fluorescence ; fluorescent dyes ; lipopolysaccharides ; mitochondria ; mitophagy ; oxidative stress ; rapamycin
    Language English
    Dates of publication 2023-0829
    Size p. 13668-13673.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.3c02717
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  7. Article ; Online: A Reaction-Based Ratiometric Bioluminescent Platform for Point-of-Care and Quantitative Detection Using a Smartphone

    Li, Junbin / Wang, Na / Xiong, Mengyi / Dai, Min / Xie, Cheng / Wang, Qianqian / Quan, Ke / Zhou, Yibo / Qing, Zhihe

    Analytical Chemistry. 2023 Apr. 25, v. 95, no. 18 p.7142-7149

    2023  

    Abstract: Fluorescent probes have emerged as powerful tools for the detection of different analytes by virtue of structural tenability. However, the requirement of an excitation source largely hinders their applicability in point-of-care detection, as well as ... ...

    Abstract Fluorescent probes have emerged as powerful tools for the detection of different analytes by virtue of structural tenability. However, the requirement of an excitation source largely hinders their applicability in point-of-care detection, as well as causing autofluorescence interference in complex samples. Herein, based on bioluminescence resonance energy transfer (BRET), we developed a reaction-based ratiometric bioluminescent platform, which allows the excitation-free detection of analytes. The platform has a modular design consisting of a NanoLuc-HaloTag fusion as an energy donor, to which a synthetic fluorescent probe is bioorthogonally labeled as recognition moiety and energy acceptor. Once activated by the target, the fluorescent probe can be excited by NanoLuc to generate a remarkable BRET signal, resulting in obvious color changes of luminescence, which can be easily recorded and quantitatively analyzed by a smartphone. As a proof of concept, a fluorescent probe for HOCl was synthesized to construct the bioluminescent system. Results demonstrated the system showed a constant blue/red emission ratio which is independent to the signal intensity, allowing the quantification of HOCl concentration with high sensitivity (limit of detection (LOD) = 13 nM) and accuracy. Given the universality, this reaction-based bioluminescent platform holds great potential for point-of-care and quantitative detection of reactive species.
    Keywords analytical chemistry ; bioluminescence ; chemical species ; color ; detection limit ; energy transfer ; fluorescence ; fluorescent dyes ; mobile telephones ; moieties ; point-of-care systems
    Language English
    Dates of publication 2023-0425
    Size p. 7142-7149.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05422
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  8. Article: Editorial: Advances in Nucleic Acid-Based Biosensors and Imaging.

    Choi, Jane Ru / Somasundrum, Mithran / Shiddiky, Muhammad J A / Surareungchai, Werasak / Hu, Yufang / Qing, Zhihe

    Frontiers in chemistry

    2022  Volume 10, Page(s) 925082

    Language English
    Publishing date 2022-05-24
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2711776-5
    ISSN 2296-2646
    ISSN 2296-2646
    DOI 10.3389/fchem.2022.925082
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  9. Article ; Online: A persistent luminescent nanobeacon for practical detection of lead ions via avoiding background interference.

    Luo, Qi / Qin, Lingfeng / Zhang, Peng / Feng, Bo / Ye, Xiaosheng / Qing, Taiping / Qing, Zhihe

    Analytica chimica acta

    2022  Volume 1198, Page(s) 339555

    Abstract: Heavy metal ions are considered to be the most serious sources for water pollution. Accurate detection of metal ions is important for pollution control and ecological protection. Background interference is an inevitable obstacle in the fluorescent ... ...

    Abstract Heavy metal ions are considered to be the most serious sources for water pollution. Accurate detection of metal ions is important for pollution control and ecological protection. Background interference is an inevitable obstacle in the fluorescent analysis of complex samples. Herein, a persistent luminescent nanobeacon is communicated to detect metal ions in real samples via avoiding background interference. The nanobeacon is constituted by persistent luminescence nanomaterials and metal-specific DNAzymes. As a proof of concept, Zn
    MeSH term(s) Biosensing Techniques/methods ; DNA, Catalytic ; Ions ; Lead ; Luminescence
    Chemical Substances DNA, Catalytic ; Ions ; Lead (2P299V784P)
    Language English
    Publishing date 2022-01-26
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2022.339555
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: A Reaction-Based Ratiometric Bioluminescent Platform for Point-of-Care and Quantitative Detection Using a Smartphone.

    Li, Junbin / Wang, Na / Xiong, Mengyi / Dai, Min / Xie, Cheng / Wang, Qianqian / Quan, Ke / Zhou, Yibo / Qing, Zhihe

    Analytical chemistry

    2023  Volume 95, Issue 18, Page(s) 7142–7149

    Abstract: Fluorescent probes have emerged as powerful tools for the detection of different analytes by virtue of structural tenability. However, the requirement of an excitation source largely hinders their applicability in point-of-care detection, as well as ... ...

    Abstract Fluorescent probes have emerged as powerful tools for the detection of different analytes by virtue of structural tenability. However, the requirement of an excitation source largely hinders their applicability in point-of-care detection, as well as causing autofluorescence interference in complex samples. Herein, based on bioluminescence resonance energy transfer (BRET), we developed a reaction-based ratiometric bioluminescent platform, which allows the excitation-free detection of analytes. The platform has a modular design consisting of a NanoLuc-HaloTag fusion as an energy donor, to which a synthetic fluorescent probe is bioorthogonally labeled as recognition moiety and energy acceptor. Once activated by the target, the fluorescent probe can be excited by NanoLuc to generate a remarkable BRET signal, resulting in obvious color changes of luminescence, which can be easily recorded and quantitatively analyzed by a smartphone. As a proof of concept, a fluorescent probe for HOCl was synthesized to construct the bioluminescent system. Results demonstrated the system showed a constant blue/red emission ratio which is independent to the signal intensity, allowing the quantification of HOCl concentration with high sensitivity (limit of detection (LOD) = 13 nM) and accuracy. Given the universality, this reaction-based bioluminescent platform holds great potential for point-of-care and quantitative detection of reactive species.
    MeSH term(s) Fluorescent Dyes/chemistry ; Smartphone ; Point-of-Care Systems ; Energy Transfer ; Immunologic Tests
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2023-04-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05422
    Database MEDical Literature Analysis and Retrieval System OnLINE

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