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  1. Article ; Online: Diabetes as a potential compounding factor in COVID-19-mediated male subfertility

    Qingkui Jiang / Thomas Linn / Karl Drlica / Lanbo Shi

    Cell & Bioscience, Vol 12, Iss 1, Pp 1-

    2022  Volume 10

    Abstract: Abstract Recent work indicates that male fertility is compromised by SARS-CoV-2 infection. Direct effects derive from the presence of viral entry receptors (ACE2 and/or CD147) on the surface of testicular cells, such as spermatocytes, Sertoli cells, and ... ...

    Abstract Abstract Recent work indicates that male fertility is compromised by SARS-CoV-2 infection. Direct effects derive from the presence of viral entry receptors (ACE2 and/or CD147) on the surface of testicular cells, such as spermatocytes, Sertoli cells, and Leydig cells. Indirect effects on testis and concentrations of male reproductive hormones derive from (1) virus-stimulated inflammation; (2) viral-induced diabetes, and (3) an interaction between diabetes and inflammation that exacerbates the deleterious effect of each perturbation. Reproductive hormones affected include testosterone, luteinizing hormone, and follicle-stimulating hormone. Reduction of male fertility is also observed with other viral infections, but the global pandemic of COVID-19 makes demographic and public health implications of reduced male fertility of major concern, especially if it occurs in the absence of serious symptoms that would otherwise encourage vaccination. Clinical documentation of COVID-19-associated male subfertility is now warranted to obtain quantitative relationships between infection severity and subfertility; mechanistic studies using animal models may reveal ways to mitigate the problem. In the meantime, the possibility of subfertility due to COVID-19 should enter considerations of vaccine hesitancy by reproductive-age males.
    Keywords SARS-CoV-2 ; Inflammation ; Diabetes ; Spermatogenesis ; Male reproductive hormones ; Male subfertility ; Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Subject code 590
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Application of formulated diets and their effects on nutrient digestibility and reproductive performance of female mink (Neovison vison) during gestation

    Qingkui Jiang / Guangyu Li / Tietao Zhang / Haihua Zhang / Xiuhua Gao / Xiumei Xing / Fuhe Yang

    Journal of Applied Animal Research, Vol 46, Iss 1, Pp 125-

    2018  Volume 129

    Abstract: This study was designed to investigate the possibility of total replacement of conventional diet by formulated experimental feed in farmed mink during gestation. Ninety female mink (Neovison vison) were randomly assigned to three groups: control group ... ...

    Abstract This study was designed to investigate the possibility of total replacement of conventional diet by formulated experimental feed in farmed mink during gestation. Ninety female mink (Neovison vison) were randomly assigned to three groups: control group was fed conventional diet with normal (36%, C) protein level composed of fresh or frozen animal by-products and extruded corn. Formulated experimental diets contained animal meals, vegetable ingredients and fat, with normal (36%, E1) or high (44%, E2) protein level, and were mixed with water prior to administration. Nutrient digestibility, nitrogen (N)-balance and reproductive performance were determined to compare the effect of diets fed to female mink during gestation. Mink fed both experimental diets had lower nutrient digestibility than the control. Higher barren females, decreased birth survival rate and birth weight were noted for the two experimental groups, especially diet E1 with normal dietary protein, indicating impaired reproduction performance, but signs of improvement were seen in the experimental group with high protein level (44%, E2).
    Keywords Mink ; protein level ; formulated diet ; gestation ; reproductive performance ; Veterinary medicine ; SF600-1100
    Subject code 590
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Development of a novel human CD147 knock-in NSG mouse model to test SARS-CoV-2 viral infection

    Saiaditya Badeti / Qingkui Jiang / Alireza Naghizadeh / Hsiang-chi Tseng / Yuri Bushkin / Salvatore A. E. Marras / Annuurun Nisa / Sanjay Tyagi / Fei Chen / Peter Romanienko / Ghassan Yehia / Deborah Evans / Moises Lopez-Gonzalez / David Alland / Riccardo Russo / William Gause / Lanbo Shi / Dongfang Liu

    Cell & Bioscience, Vol 12, Iss 1, Pp 1-

    2022  Volume 19

    Abstract: Abstract Background An animal model that can mimic the SARS-CoV-2 infection in humans is critical to understanding the rapidly evolving SARS-CoV-2 virus and for development of prophylactic and therapeutic strategies to combat emerging mutants. Studies ... ...

    Abstract Abstract Background An animal model that can mimic the SARS-CoV-2 infection in humans is critical to understanding the rapidly evolving SARS-CoV-2 virus and for development of prophylactic and therapeutic strategies to combat emerging mutants. Studies show that the spike proteins of SARS-CoV and SARS-CoV-2 bind to human angiotensin-converting enzyme 2 (hACE2, a well-recognized, functional receptor for SARS-CoV and SARS-CoV-2) to mediate viral entry. Several hACE2 transgenic (hACE2Tg) mouse models are being widely used, which are clearly invaluable. However, the hACE2Tg mouse model cannot fully explain: (1) low expression of ACE2 observed in human lung and heart, but lung or heart failure occurs frequently in severe COVID-19 patients; (2) low expression of ACE2 on immune cells, but lymphocytopenia occurs frequently in COVID-19 patients; and (3) hACE2Tg mice do not mimic the natural course of SARS-CoV-2 infection in humans. Moreover, one of most outstanding features of coronavirus infection is the diversity of receptor usage, which includes the newly proposed human CD147 (hCD147) as a possible co-receptor for SARS-CoV-2 entry. It is still debatable whether CD147 can serve as a functional receptor for SARS-CoV-2 infection or entry. Results Here we successfully generated a hCD147 knock-in mouse model (hCD147KI) in the NOD-scid IL2Rgammanull (NSG) background. In this hCD147KI-NSG mouse model, the hCD147 genetic sequence was placed downstream of the endogenous mouse promoter for mouse CD147 (mCD147), which creates an in vivo model that may better recapitulate physiological expression of hCD147 proteins at the molecular level compared to the existing and well-studied K18-hACE2-B6 (JAX) model. In addition, the hCD147KI-NSG mouse model allows further study of SARS-CoV-2 in the immunodeficiency condition which may assist our understanding of this virus in the context of high-risk populations in immunosuppressed states. Our data show (1) the human CD147 protein is expressed in various organs (including bronchiolar ...
    Keywords CD147 ; Basigin ; BSG ; hCD147KI ; NSG ; SARS-CoV-2 ; Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Subject code 616
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Elevated CCL2 causes Leydig cell malfunction in metabolic syndrome

    Qingkui Jiang / Constanze C. Maresch / Sebastian Friedrich Petry / Agnieszka Paradowska-Dogan / Sudhanshu Bhushan / Yongsheng Chang / Christine Wrenzycki / Hans-Christian Schuppe / Petr Houska / Michaela F. Hartmann / Stefan A. Wudy / Lanbo Shi / Thomas Linn

    JCI Insight, Vol 5, Iss

    2020  Volume 21

    Abstract: Metabolic syndrome (MetS), which is associated with chronic inflammation, predisposes males to hypogonadism and subfertility. The underlying mechanism of these pathologies remains poorly understood. Homozygous leptin-resistant obese db/db mice are ... ...

    Abstract Metabolic syndrome (MetS), which is associated with chronic inflammation, predisposes males to hypogonadism and subfertility. The underlying mechanism of these pathologies remains poorly understood. Homozygous leptin-resistant obese db/db mice are characterized by small testes, low testicular testosterone, and a reduced number of Leydig cells. Here we report that IL-1β, CCL2 (also known as MCP-1), and corticosterone concentrations were increased in the testes of db/db mice relative to those in WT controls. Cultured murine and human Leydig cells responded to cytokine stress with increased CCL2 release and apoptotic signals. Chemical inhibition of CCL2 rescued Leydig cell function in vitro and in db/db mice. Consistently, we found that Ccl2-deficient mice fed with a high-energy diet were protected from testicular dysfunction compared with similarly fed WT mice. Finally, a cohort of infertile men with a history of MetS showed that reduction of CCL2 plasma levels could be achieved by weight loss and was clearly associated with recovery from hypogonadism. Taken together, we conclude that CCL2-mediated chronic inflammation is, to a large extent, responsible for the subfertility in MetS by causing damage to Leydig cells.
    Keywords Endocrinology ; Reproductive biology ; Medicine ; R
    Language English
    Publishing date 2020-11-01T00:00:00Z
    Publisher American Society for Clinical investigation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Low testosterone in ApoE/LDL receptor double-knockout mice is associated with rarefied testicular capillaries together with fewer and smaller Leydig cells

    Kai Steinfeld / Daniela Beyer / Christian Mühlfeld / Andrea Mietens / Gerrit Eichner / Bora Altinkilic / Marian Kampschulte / Qingkui Jiang / Gabriele A. Krombach / Thomas Linn / Wolfgang Weidner / Ralf Middendorff

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 13

    Abstract: Abstract The testis as a site for atherosclerotic changes has so far attracted little attention. We used the apolipoprotein E (ApoE)/low density lipoprotein (LDL) receptor deficient mouse model (KO) for atherosclerosis (20, 40, 60 and 87-week-old) to ... ...

    Abstract Abstract The testis as a site for atherosclerotic changes has so far attracted little attention. We used the apolipoprotein E (ApoE)/low density lipoprotein (LDL) receptor deficient mouse model (KO) for atherosclerosis (20, 40, 60 and 87-week-old) to investigate whether Leydig cells or the capillary network are responsible for reduced serum testosterone levels previously observed in extreme ages of this model. In KO mice, overall testosterone levels were reduced whereas the adrenal gland-specific corticosterone was increased excluding a general defect of steroid hormone production. In addition to micro-CT investigations for bigger vessels, stereology revealed a reduction of capillary length, volume and surface area suggesting capillary rarefaction as a factor for diminished testosterone. Stereological analyses of interstitial cells demonstrated significantly reduced Leydig cell numbers and size. These structural changes in the testis occurred on an inflammatory background revealed by qPCR. Reduced litter size of the KO mice suggests hypo- or infertility as a consequence of the testicular defects. Our data suggest reduced testosterone levels in this atherosclerosis model might be explained by both, rarefication of the capillary network and reduced Leydig cell number and size. Thus, this study calls for specific treatment of male infertility induced by microvascular damage through hypercholesterolemia and atherosclerosis.
    Keywords Medicine ; R ; Science ; Q
    Subject code 616
    Language English
    Publishing date 2018-04-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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