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  1. Article ; Online: The comparison of self-gripping mesh and conventional mesh in laparoscopic inguinal hernia repair: the results of meta-analysis.

    Wang, Dianchen / Jiang, Jianwu / Fu, Yang / Qu, Pan

    Updates in surgery

    2022  Volume 74, Issue 3, Page(s) 857–863

    Abstract: Self-gripping mesh is widely used in laparoscopic inguinal hernia repair and some researches report its advantages compared with conventional mesh. The aim of this study was to assess outcomes of self-gripping mesh and conventional mesh in laparoscopic ... ...

    Abstract Self-gripping mesh is widely used in laparoscopic inguinal hernia repair and some researches report its advantages compared with conventional mesh. The aim of this study was to assess outcomes of self-gripping mesh and conventional mesh in laparoscopic inguinal hernia repair. A systematic literature review was undertaken to identify studies comparing the results of self-gripping mesh and conventional mesh in laparoscopic inguinal hernia repair. Outcomes, including recurrence, chronic pain, operation time, hematoma, seroma and infection, were measured. Four randomized controlled trials and 1 prospective comparative study were analyzed. The incidence of chronic pain in self-gripping group was significantly lower than that in conventional group (OR 0.43, 95% CI 0.20, 0.93, P = 0.03), and there was no significant difference in hernia recurrence (OR 0.31, 95% CI 0.03, 3.06, P = 0.32), operation time (MD 0.06, 95%CI - 2.32, 2.44, P = 0.96), hematoma (OR 1.01, 95% CI 0.33, 3.07, P = 0.99) and seroma (OR 0.90, 95% CI 0.49, 1.66, P = 0.73). Laparoscopic inguinal hernia repair using self-gripping mesh is associated with a decreased incidence of chronic pain compared with conventional mesh, without increased postoperative complications.
    MeSH term(s) Chronic Pain/complications ; Chronic Pain/surgery ; Hematoma/epidemiology ; Hematoma/etiology ; Hernia, Inguinal/surgery ; Herniorrhaphy/methods ; Humans ; Laparoscopy/methods ; Pain, Postoperative/etiology ; Prospective Studies ; Recurrence ; Seroma/complications ; Seroma/etiology ; Surgical Mesh
    Language English
    Publishing date 2022-01-16
    Publishing country Italy
    Document type Journal Article ; Meta-Analysis ; Review ; Systematic Review
    ZDB-ID 2572692-4
    ISSN 2038-3312 ; 2038-131X
    ISSN (online) 2038-3312
    ISSN 2038-131X
    DOI 10.1007/s13304-021-01218-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Provenances of the Oldest Xiashu loess and Xuancheng loess in southern China: Implications for dust transport and dust proxies of the East Asian winter monsoon

    Wu, Chao / Zheng, Xiangmin / Qian, Peng / Qu, Pan / He, Miaohong / Wei, Gangjian

    Palaeogeography, Palaeoclimatology, Palaeoecology. 2023 Mar., v. 614 p.111440-

    2023  

    Abstract: The loess-paleosol sequences of southern China are valuable paleoclimatic archives since the Middle Pleistocene, on the regional and global scales. Knowledge of loess provenance can shed light on dust transport and the development of paleoclimatic ... ...

    Abstract The loess-paleosol sequences of southern China are valuable paleoclimatic archives since the Middle Pleistocene, on the regional and global scales. Knowledge of loess provenance can shed light on dust transport and the development of paleoclimatic proxies for loess deposits. However, there is a research gap regarding the provenance of the Oldest (∼0.9 Ma) Xiashu loess near the Yangtze River valley. The precise source area of the Xuancheng loess in southern China remains to be unidentified. In this study, we used elemental geochemistry and detrital zircon UPb ages to identify the sources of the Oldest Xiashu loess and Xuancheng loess in southern China. The Oldest Xiashu loess is dominantly derived from Yangtze River sediments, and part of its fine-grained dust component originates from the arid regions of Central Asia or northern China. The elemental compositions of the size-fractionated silicates and detrital zircon UPb ages of the Xuancheng loess are like those of the Shuiyang River sediments in the Xuancheng region. These results demonstrate that the Xuancheng loess is mainly the product of local dust activity in the Xuancheng regions, and thus that the Xiashu and Xuancheng loess are the products of independent dust transport processes. This spatial differentiation of loess provenance may be a widespread phenomenon in southern China, and consequently, the grain size compositions of the various loess sections in southern China cannot be used to recover paleo-wind directions on a regional scale. Overall, our study shows that when the grain size compositions from the southern China loess are used to reconstruct East Asian winter monsoon intensity, the signals from local wind fields should first be removed.
    Keywords Pleistocene epoch ; dust ; geochemistry ; loess ; monsoon season ; palaeogeography ; paleoclimatology ; paleoecology ; provenance ; river valleys ; rivers ; spatial variation ; wind ; zircon ; Central Asia ; China ; Yangtze River ; Xiashu loess ; Xuancheng loess ; Zircon UPb age ; Elemental geochemistry
    Language English
    Dates of publication 2023-03
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 417718-6
    ISSN 0031-0182
    ISSN 0031-0182
    DOI 10.1016/j.palaeo.2023.111440
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Lnc-NEAT1 induces cell apoptosis and inflammation but inhibits proliferation in a cellular model of hepatic ischemia/reperfusion injury.

    Wang, Liu / Qu, Pan / Yin, Wanling / Sun, Jiao

    The Journal of international medical research

    2021  Volume 49, Issue 3, Page(s) 300060519887251

    Abstract: Objective: We aimed to investigate the effect of long non-coding RNA nuclear-enriched abundant transcript 1 (lnc-NEAT1) on regulating hepatocyte proliferation, apoptosis, and inflammation during hepatic ischemia/reperfusion (I/R) injury.: Methods: ... ...

    Abstract Objective: We aimed to investigate the effect of long non-coding RNA nuclear-enriched abundant transcript 1 (lnc-NEAT1) on regulating hepatocyte proliferation, apoptosis, and inflammation during hepatic ischemia/reperfusion (I/R) injury.
    Methods: Human liver cells (HL-7702) were cultured under glucose-free and oxygen-free conditions to construct the I/R injury model. Expression of lnc-NEAT1 was detected in this model and in normal cells. Plasmids of control overexpression [NC(+)], lnc-NEAT1 overexpression [NEAT1(+)], control short hairpin (sh)RNA [NC(-)], and lnc-NEAT1 shRNA [NEAT1(-)] were transfected into HL-7702 cells and subsequently subjected to I/R treatment. Cell proliferation, apoptosis, apoptosis-related proteins, and inflammatory cytokines were assessed.
    Results: Lnc-NEAT1 expression was elevated in the I/R group compared with the normal group. Cell proliferation was decreased in the NEAT1(+) group compared with the NC(+) group but increased in NEAT1(-) compared with NC(-). The apoptosis rate increased in the NEAT1(+) group compared with the NC(+) group but decreased in NEAT1(-) compared with NC(-). Western blot assay (detection of apoptosis-related proteins) showed similar results. Expression of interleukin-1β, interleukin-6, and tumor necrosis factor-α increased in the NEAT1(+) group compared with NC(+) but decreased in NEAT1(-) compared with NC(-).
    Conclusion: Lnc-NEAT1 is overexpressed, induces cell apoptosis and inflammation, and inhibits proliferation during hepatic I/R injury.
    MeSH term(s) Apoptosis ; Cell Line ; Cell Proliferation ; Humans ; Inflammation/genetics ; Ischemia ; Liver ; RNA, Long Noncoding/genetics ; Reperfusion Injury/genetics
    Chemical Substances NEAT1 long non-coding RNA, human ; RNA, Long Noncoding
    Language English
    Publishing date 2021-03-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 184023-x
    ISSN 1473-2300 ; 0300-0605 ; 0142-2596
    ISSN (online) 1473-2300
    ISSN 0300-0605 ; 0142-2596
    DOI 10.1177/0300060519887251
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Lactobacillus futsaii subsp. chongqingii subsp. nov., Isolated from a Traditional Chinese Pickle

    Du, Xin / Kang Cao / Mingzheng Tan / Qu Pan

    Current microbiology. 2019 Feb., v. 76, no. 2

    2019  

    Abstract: Strain CQ16Z1ᵀ was isolated from jamiecosley, a traditional Chinese pickle. The isolate was Gram-positive, non-motile, non-spore-forming, facultatively anaerobic, catalase-negative, and long rod-shaped. The optimal temperature for growth was 37 °C and ... ...

    Abstract Strain CQ16Z1ᵀ was isolated from jamiecosley, a traditional Chinese pickle. The isolate was Gram-positive, non-motile, non-spore-forming, facultatively anaerobic, catalase-negative, and long rod-shaped. The optimal temperature for growth was 37 °C and the DNA G + C content was 39.1 mol%. The results of 16S rRNA and rpoA gene sequencing, DNA–DNA hybridization, and peptidoglycan type analyses indicated that strain CQ16Z1ᵀ belongs to the recognized species Lactobacillus futsaii. However, the analysis results of pheS gene sequencing, amplified fragment length polymorphism, phenotypic profiles, cellular fatty acid, cell-wall monosaccharide determination, and cell morphology revealed that the novel strain was obviously different from the type strain L. futsaii JCM17355ᵀ, and had genetic relationship with Weissella cibaria to a certain degree, suggesting that the novel strain represents a novel subspecies of L. futsaii, for which the following names are proposed: L. futsaii subsp. futsaii subsp. nov. (type strain YM0097ᵀ = JCM 17355ᵀ = BCRC 80278ᵀ) and L. futsaii subsp. chongqingii subsp.nov., with the type strain CQ16Z1ᵀ (= CCTCC AB2017187ᵀ = KCTC 21089ᵀ).
    Keywords DNA ; Lactobacillus ; Weissella cibaria ; cell biology ; fatty acids ; genetic relationships ; new subspecies ; nucleic acid hybridization ; peptidoglycans ; phenotype ; pickles ; ribosomal RNA ; sequence analysis ; temperature
    Language English
    Dates of publication 2019-02
    Size p. 153-158.
    Publishing place Springer US
    Document type Article
    ZDB-ID 134238-1
    ISSN 1432-0991 ; 0343-8651
    ISSN (online) 1432-0991
    ISSN 0343-8651
    DOI 10.1007/s00284-018-1601-2
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Randomized Trial Comparing Self-Gripping Mesh with Polypropylene Mesh in Female Lichtenstein Hernioplasty.

    Wang, Dianchen / Zhang, Hui / Lei, Ting / Chen, Jianmin / Chen, Yake / Zhang, Yi / Qu, Pan

    The American surgeon

    2020  Volume 86, Issue 2, Page(s) 110–115

    Abstract: Several randomized trials comparing self-gripping mesh with polypropylene (PL) mesh in Lichtenstein hernioplasty revealed that the self-gripping mesh significantly reduced the operation time. In these studies, some enrolled only male patients, and in ... ...

    Abstract Several randomized trials comparing self-gripping mesh with polypropylene (PL) mesh in Lichtenstein hernioplasty revealed that the self-gripping mesh significantly reduced the operation time. In these studies, some enrolled only male patients, and in others, the proportion of women was extremely low. The aim of this research was to compare outcomes after self-gripping mesh repair with PL mesh secured with sutures in female Lichtenstein hernioplasty. Female patients with primary unilateral inguinal hernia were assigned randomly to undergo Lichtenstein hernioplasty with a self-gripping ProGrip (PG) mesh or a sutured PL mesh, followed-up at one week, one month, three months, one year, and two years. Demographics, hernia characteristics, and operative outcomes data were analyzed. Pain was assessed with a visual analog scale (0-10), and quality of life (QOL) was estimated by a 36-item short-form general survey (0-26). Forty eight patients in the PG group and 51 participants in the PL group completed the follow-up. The operation time of the PG (54.1 ± 12 minutes) group was significantly shorter than that of the PL (60.9 ± 11.3 minutes) group (
    MeSH term(s) Adult ; Double-Blind Method ; Female ; Follow-Up Studies ; Foreign Bodies/diagnosis ; Hernia, Inguinal/surgery ; Herniorrhaphy/instrumentation ; Herniorrhaphy/methods ; Humans ; Operative Time ; Pain Measurement/methods ; Pain, Postoperative/diagnosis ; Polypropylenes ; Prospective Studies ; Quality of Life ; Recurrence ; Surgical Mesh/adverse effects ; Sutures ; Time Factors ; Treatment Outcome
    Chemical Substances Polypropylenes
    Language English
    Publishing date 2020-03-12
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Randomized Controlled Trial
    ZDB-ID 202465-2
    ISSN 1555-9823 ; 0003-1348
    ISSN (online) 1555-9823
    ISSN 0003-1348
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Detection and characterization of a theta-replicating plasmid pLP60 from Lactobacillus plantarum PC518 by inverse PCR

    Fang Yao / XiaoYu Xu / Xin Du / Kang Cao / Qu Pan

    Heliyon, Vol 5, Iss 8, Pp e02164- (2019)

    2019  

    Abstract: Plasmid DNA of Lactobacillus plantarum PC518 was isolated by an improved method which contained a washing step for removing lysozyme. Three plasmid DNA libraries were constructed. A pair of outward primers was designed at both ends of the novel plasmid ... ...

    Abstract Plasmid DNA of Lactobacillus plantarum PC518 was isolated by an improved method which contained a washing step for removing lysozyme. Three plasmid DNA libraries were constructed. A pair of outward primers was designed at both ends of the novel plasmid fragment obtained from plasmid DNA libraries, and the remainder of the circle plasmid was amplified by inverse PCR (iPCR). The whole sequence of plasmid was analyzed by the basic local alignment search tool, Tandem Repeats Finder, DNAMAN V6.0, DNASTAR and MEGA X software. The copy number was measured using quantitative real-time PCR. Plasmid extract showed 7 bands on agarose gel, indicating that L. plantarum PC518 contains multiple plasmids. The complete sequence of plasmid pLP60 was obtained by plasmid DNA libraries and iPCR. pLP60 is 6006 bp in length with a G + C content of 41.19 %, which encodes 8 open reading frames (ORFs). The ori site like theta-type could be located upstream of repB, which contains a short tandem repeats (sTR) and a long tandem repeats (lTR). RepB of pLP60 only had low similarity with Rep protein of known theta-type plasmids, but phylogenetic tree analysis showed that plasmids whose Rep proteins are similar to pLP60 have lTR at ori, and the conservativeness of lTR is consistent with similarity of Rep proteins, suggesting that RepB of pLP60 is a theta-replicating protein. So pLP60 was classified as class A of theta replication. The copy number of pLP60 was measured as 5 copies per cell by qPCR.
    Keywords Microbiology ; Genetics ; Molecular biology ; Bacterial genetics ; Microbial genomics ; Microbial biotechnology ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Subject code 572
    Language English
    Publishing date 2019-08-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Epidemiological Features of 105 Patients Infected with the COVID-19

    Shi, Ji-Chan Yu Zhi-Jie He Gui-Qing Chen Wei Ye Xin-Chun Wu Zheng-Xing Zhu Xiao-Qu Pan Jiao-Zhi Jiang Xian-Gao

    Journal of the National Medical Association

    Abstract: Objective To investigate and evaluate the clinical features of patients infected with the 2019 novel coronavirus (COVID-19) outside of Wuhan Methods 105 patients admitted to our hospital with clinical- and laboratory-confirmed COVID-19 infection were ... ...

    Abstract Objective To investigate and evaluate the clinical features of patients infected with the 2019 novel coronavirus (COVID-19) outside of Wuhan Methods 105 patients admitted to our hospital with clinical- and laboratory-confirmed COVID-19 infection were studied Data were collected from January 17, 2020 to March 5, 2020 Results 105 patients (57 male and 48 female) were confirmed to have COVID-19 infection Among the 105 patients, 55 (52%) had made short trips to Wuhan during the two weeks before the onset of illness, and these were the first-generation confirmed cases An exact date of close contact with someone in Wenzhou with confirmed or suspected COVID-19 infection from Wuhan (the second-generation confirmed cases) could be provided by 38 (36%) patients Of the remaining patients, six (6%;the third-generation confirmed cases) were familial clusters of the second-generation confirmed cases, three (3%) had no definite epidemiological features, and 16 (15%) were from the same location as for the case report Conclusion Due to the infectiousness of COVID-19, patients with infections should be diagnosed and treated as early as possible after developing fever symptoms or showing other clinical characteristics or imaging features With respect to high-risk cases, we must focus on any complications that arise and take effective measures to treat them immediately This will significantly improve the prognosis of patients with severe infections
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #894055
    Database COVID19

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