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  1. Article: Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation.

    Qu, Yongcun / Zhang, Jia / Guo, Shanshan / Zhang, Liwen / Qian, Jingjing / Zhu, Xili / Duan, Enkui / Zhang, Ying

    Frontiers in cell and developmental biology

    2022  Volume 10, Page(s) 933852

    Abstract: Uterine status determines pregnancy success. Although it is well known that superovulation operations can disrupt uterine function, our understanding of the morphological changes in the uterine endometrium at the three-dimensional (3D) level is limited. ... ...

    Abstract Uterine status determines pregnancy success. Although it is well known that superovulation operations can disrupt uterine function, our understanding of the morphological changes in the uterine endometrium at the three-dimensional (3D) level is limited. Here, combining the tissue clearing with 3D deep imaging, we reveal an increase in epithelial density and angiogenesis after ovarian stimulation, which is accompanied by a circulating surge in P4 levels. Using an ovariectomized mouse model, we further detected the separate regulatory effects of P4 and E2 on the uterine endometrium, with P4 promoting endothelial cell growth and E2 inducing epithelial proliferation. Additionally, we observed that the effects of E2 can be partially neutralized by P4, and vice versa. By analyzing the 3D uterine imaging, we discovered an interesting phenomenon in which the growing blood vessels closely surround the remodeling uterine epithelium, indicating a close relationship between angiogenesis and epithelial growth. These findings provide new insight into the uterine epithelial changes and angiogenesis at the 3D level, and explain a potential reason for endometrial changes due to the low implantation rate in patients undergoing clinic super-ovulation.
    Language English
    Publishing date 2022-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2022.933852
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: RNA Modification Signature of Peripheral Blood as a Potential Diagnostic Marker for Pulmonary Hypertension.

    Zhang, Liwen / Li, Yuzhu / Wang, Jian / Hong, Cheng / Lu, Wenju / Qu, Yongcun / Black, Stephen M / Chen, Qi / Zhou, Tong / Jin, Wanzhu / Duan, Enkui / Tang, Haiyang / Zhang, Ying

    Hypertension (Dallas, Tex. : 1979)

    2022  Volume 79, Issue 3, Page(s) e67–e69

    MeSH term(s) Biomarkers/blood ; Humans ; Hypertension, Pulmonary/blood ; Hypertension, Pulmonary/diagnosis ; RNA Processing, Post-Transcriptional
    Chemical Substances Biomarkers
    Language English
    Publishing date 2022-01-11
    Publishing country United States
    Document type Letter ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 423736-5
    ISSN 1524-4563 ; 0194-911X ; 0362-4323
    ISSN (online) 1524-4563
    ISSN 0194-911X ; 0362-4323
    DOI 10.1161/HYPERTENSIONAHA.121.18724
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Development of mouse preimplantation embryos in space.

    Lei, Xiaohua / Cao, Yujing / Ma, Baohua / Zhang, Yunfang / Ning, Lina / Qian, Jingjing / Zhang, Liwen / Qu, Yongcun / Zhang, Tao / Li, Dehong / Chen, Qi / Shi, Junchao / Zhang, Xudong / Ma, Chiyuan / Zhang, Ying / Duan, Enkui

    National science review

    2020  Volume 7, Issue 9, Page(s) 1437–1446

    Abstract: The development of life beyond planet Earth is a long-standing quest of the human race, but whether normal mammalian embryonic development can occur in space is still unclear. Here, we show unequivocally that preimplantation mouse embryos can develop in ... ...

    Abstract The development of life beyond planet Earth is a long-standing quest of the human race, but whether normal mammalian embryonic development can occur in space is still unclear. Here, we show unequivocally that preimplantation mouse embryos can develop in space, but the rate of blastocyst formation and blastocyst quality are compromised. Additionally, the cells in the embryo contain severe DNA damage, while the genome of the blastocysts developed in space is globally hypomethylated with a unique set of differentially methylated regions. The developmental defects, DNA damage and epigenetic abnormalities can be largely mimicked by the treatment with ground-based low-dose radiation. However, the exposure to simulated microgravity alone does not cause major disruptions of embryonic development, indicating that radiation is the main cause for the developmental defects. This work advances the understanding of embryonic development in space and reveals long-term extreme low-dose radiation as a hazardous factor for mammalian reproduction.
    Language English
    Publishing date 2020-04-11
    Publishing country China
    Document type Journal Article
    ZDB-ID 2745465-4
    ISSN 2053-714X ; 2053-714X
    ISSN (online) 2053-714X
    ISSN 2053-714X
    DOI 10.1093/nsr/nwaa062
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  4. Article ; Online: Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization.

    Qu, Yongcun / Chen, Qi / Guo, Shanshan / Ma, Chiyuan / Lu, Yonggang / Shi, Junchao / Liu, Shichao / Zhou, Tong / Noda, Taichi / Qian, Jingjing / Zhang, Liwen / Zhu, Xili / Lei, Xiaohua / Cao, Yujing / Li, Wei / Plachta, Nicolas / Matzuk, Martin M / Ikawa, Masahito / Duan, Enkui /
    Zhang, Ying / Wang, Hongmei

    Protein & cell

    2021  Volume 12, Issue 10, Page(s) 810–817

    MeSH term(s) Animals ; Antigens, Surface/genetics ; Cell Communication/genetics ; Copulation/physiology ; Fallopian Tubes/anatomy & histology ; Fallopian Tubes/metabolism ; Female ; Fertilization/genetics ; GPI-Linked Proteins/deficiency ; GPI-Linked Proteins/genetics ; Gene Expression Regulation ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Litter Size ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mitochondria/metabolism ; Reproduction/genetics ; Signal Transduction ; Sperm Count ; Sperm Motility/genetics ; Spermatozoa/metabolism ; Uterus/anatomy & histology ; Uterus/metabolism
    Chemical Substances Antigens, Surface ; GPI-Linked Proteins ; Luminescent Proteins ; TEX101 protein, mouse ; enhanced green fluorescent protein ; fluorescent protein 583 ; Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2021-03-01
    Publishing country Germany
    Document type Letter ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2543451-2
    ISSN 1674-8018 ; 1674-800X
    ISSN (online) 1674-8018
    ISSN 1674-800X
    DOI 10.1007/s13238-021-00825-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Caffeine consumption during early pregnancy impairs oviductal embryo transport, embryonic development and uterine receptivity in mice.

    Qian, Jingjing / Zhang, Yunfang / Qu, Yongcun / Zhang, Liwen / Shi, Junchao / Zhang, Xudong / Liu, Shichao / Kim, Bo Hyun / Hwang, Sung Jin / Zhou, Tong / Chen, Qi / Ward, Sean M / Duan, Enkui / Zhang, Ying

    Biology of reproduction

    2018  Volume 99, Issue 6, Page(s) 1266–1275

    Abstract: Caffeine consumption has been widely used as a central nervous system stimulant. Epidemiological studies, however, have suggested that maternal caffeine exposure during pregnancy is associated with increased abnormalities, including decreased fertility, ... ...

    Abstract Caffeine consumption has been widely used as a central nervous system stimulant. Epidemiological studies, however, have suggested that maternal caffeine exposure during pregnancy is associated with increased abnormalities, including decreased fertility, delayed conception, early spontaneous abortions, and low birth weight. The mechanisms underlying the negative outcomes of caffeine consumption, particularly during early pregnancy, remain unclear. In present study, we found that pregnant mice treated with moderate (5 mg/kg) or high (30 mg/kg) dosage of caffeine (intraperitoneally or orally) during preimplantation resulted in retention of early embryos in the oviduct, defective embryonic development, and impaired embryo implantation. Transferring normal blastocysts into the uteri of caffeine-treated pseudopregnant females also showed abnormal embryo implantation, thus indicating impaired uterine receptivity by caffeine administration. The remaining embryos that managed to implant after caffeine treatment also showed increased embryo resorption rate and abnormal development at mid-term stage, and decreased weight at birth. In addition to a dose-dependent effect, significant variations between individual mice under the same caffeine dosage were also observed, suggesting different sensitivities to caffeine, similar to that observed in human populations. Collectively, our data revealed that caffeine exposure during early pregnancy impaired oviductal embryo transport, embryonic development, and uterine receptivity, which are responsible for abnormal implantation and pregnancy loss. The study raises the concern of caffeine consumption during early stages of pregnancy.
    MeSH term(s) Animals ; Caffeine/administration & dosage ; Caffeine/pharmacokinetics ; Embryo Implantation/drug effects ; Embryo, Mammalian/drug effects ; Fallopian Tubes/drug effects ; Fallopian Tubes/physiology ; Female ; Mice ; Pregnancy ; Pregnancy, Animal/drug effects ; Uterus/drug effects ; Uterus/physiology
    Chemical Substances Caffeine (3G6A5W338E)
    Language English
    Publishing date 2018-07-07
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1118-6
    ISSN 1529-7268 ; 0006-3363
    ISSN (online) 1529-7268
    ISSN 0006-3363
    DOI 10.1093/biolre/ioy155
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    Zs.A 551: Show issues Location:
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  6. Article ; Online: PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications.

    Shi, Junchao / Zhang, Yunfang / Tan, Dongmei / Zhang, Xudong / Yan, Menghong / Zhang, Ying / Franklin, Reuben / Shahbazi, Marta / Mackinlay, Kirsty / Liu, Shichao / Kuhle, Bernhard / James, Emma R / Zhang, Liwen / Qu, Yongcun / Zhai, Qiwei / Zhao, Wenxin / Zhao, Linlin / Zhou, Changcheng / Gu, Weifeng /
    Murn, Jernej / Guo, Jingtao / Carrell, Douglas T / Wang, Yinsheng / Chen, Xuemei / Cairns, Bradley R / Yang, Xiang-Lei / Schimmel, Paul / Zernicka-Goetz, Magdalena / Cheloufi, Sihem / Zhou, Tong / Chen, Qi

    Nature cell biology

    2021  Volume 23, Issue 4, Page(s) 424–436

    Abstract: Although high-throughput RNA sequencing (RNA-seq) has greatly advanced small non-coding RNA (sncRNA) discovery, the currently widely used complementary DNA library construction protocol generates biased sequencing results. This is partially due to RNA ... ...

    Abstract Although high-throughput RNA sequencing (RNA-seq) has greatly advanced small non-coding RNA (sncRNA) discovery, the currently widely used complementary DNA library construction protocol generates biased sequencing results. This is partially due to RNA modifications that interfere with adapter ligation and reverse transcription processes, which prevent the detection of sncRNAs bearing these modifications. Here, we present PANDORA-seq (panoramic RNA display by overcoming RNA modification aborted sequencing), employing a combinatorial enzymatic treatment to remove key RNA modifications that block adapter ligation and reverse transcription. PANDORA-seq identified abundant modified sncRNAs-mostly transfer RNA-derived small RNAs (tsRNAs) and ribosomal RNA-derived small RNAs (rsRNAs)-that were previously undetected, exhibiting tissue-specific expression across mouse brain, liver, spleen and sperm, as well as cell-specific expression across embryonic stem cells (ESCs) and HeLa cells. Using PANDORA-seq, we revealed unprecedented landscapes of microRNA, tsRNA and rsRNA dynamics during the generation of induced pluripotent stem cells. Importantly, tsRNAs and rsRNAs that are downregulated during somatic cell reprogramming impact cellular translation in ESCs, suggesting a role in lineage differentiation.
    MeSH term(s) DNA, Complementary/genetics ; HeLa Cells ; Humans ; MicroRNAs/genetics ; RNA Processing, Post-Transcriptional/genetics ; RNA, Ribosomal/genetics ; RNA, Small Untranslated/genetics ; RNA-Seq ; Transcriptome/genetics
    Chemical Substances DNA, Complementary ; MicroRNAs ; RNA, Ribosomal ; RNA, Small Untranslated
    Language English
    Publishing date 2021-04-05
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1474722-4
    ISSN 1476-4679 ; 1465-7392
    ISSN (online) 1476-4679
    ISSN 1465-7392
    DOI 10.1038/s41556-021-00652-7
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  7. Article ; Online: Author Correction: PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications.

    Shi, Junchao / Zhang, Yunfang / Tan, Dongmei / Zhang, Xudong / Yan, Menghong / Zhang, Ying / Franklin, Reuben / Shahbazi, Marta / Mackinlay, Kirsty / Liu, Shichao / Kuhle, Bernhard / James, Emma R / Zhang, Liwen / Qu, Yongcun / Zhai, Qiwei / Zhao, Wenxin / Zhao, Linlin / Zhou, Changcheng / Gu, Weifeng /
    Murn, Jernej / Guo, Jingtao / Carrell, Douglas T / Wang, Yinsheng / Chen, Xuemei / Cairns, Bradley R / Yang, Xiang-Lei / Schimmel, Paul / Zernicka-Goetz, Magdalena / Cheloufi, Sihem / Zhou, Tong / Chen, Qi

    Nature cell biology

    2021  Volume 23, Issue 6, Page(s) 676

    Abstract: A Correction to this paper has been published: https://doi.org/10.1038/s41556-021-00687-w. ...

    Abstract A Correction to this paper has been published: https://doi.org/10.1038/s41556-021-00687-w.
    Language English
    Publishing date 2021-05-03
    Publishing country England
    Document type Published Erratum
    ZDB-ID 1474722-4
    ISSN 1476-4679 ; 1465-7392
    ISSN (online) 1476-4679
    ISSN 1465-7392
    DOI 10.1038/s41556-021-00687-w
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  8. Article ; Online: Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs.

    Zhang, Yunfang / Zhang, Xudong / Shi, Junchao / Tuorto, Francesca / Li, Xin / Liu, Yusheng / Liebers, Reinhard / Zhang, Liwen / Qu, Yongcun / Qian, Jingjing / Pahima, Maya / Liu, Ying / Yan, Menghong / Cao, Zhonghong / Lei, Xiaohua / Cao, Yujing / Peng, Hongying / Liu, Shichao / Wang, Yue /
    Zheng, Huili / Woolsey, Rebekah / Quilici, David / Zhai, Qiwei / Li, Lei / Zhou, Tong / Yan, Wei / Lyko, Frank / Zhang, Ying / Zhou, Qi / Duan, Enkui / Chen, Qi

    Nature cell biology

    2018  Volume 20, Issue 5, Page(s) 535–540

    Abstract: The discovery of RNAs (for example, messenger RNAs, non-coding RNAs) in sperm has opened the possibility that sperm may function by delivering additional paternal information aside from solely providing the ... ...

    Abstract The discovery of RNAs (for example, messenger RNAs, non-coding RNAs) in sperm has opened the possibility that sperm may function by delivering additional paternal information aside from solely providing the DNA
    MeSH term(s) Animals ; Biomarkers/blood ; Blood Glucose/metabolism ; DNA (Cytosine-5-)-Methyltransferases/deficiency ; DNA (Cytosine-5-)-Methyltransferases/genetics ; DNA (Cytosine-5-)-Methyltransferases/metabolism ; Diet, High-Fat ; Epigenesis, Genetic ; Gene Expression Regulation, Developmental ; Gene-Environment Interaction ; Genetic Predisposition to Disease ; Glucose Metabolism Disorders/blood ; Glucose Metabolism Disorders/diagnosis ; Glucose Metabolism Disorders/enzymology ; Glucose Metabolism Disorders/genetics ; Heredity ; Insulin/blood ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; NIH 3T3 Cells ; Nucleic Acid Conformation ; Paternal Inheritance ; Phenotype ; RNA, Small Untranslated/chemistry ; RNA, Small Untranslated/genetics ; RNA, Small Untranslated/metabolism ; Spermatozoa/enzymology ; Structure-Activity Relationship ; Transcriptome
    Chemical Substances Biomarkers ; Blood Glucose ; Insulin ; RNA, Small Untranslated ; Dnmt2 protein, mouse (EC 2.1.1.-) ; DNA (Cytosine-5-)-Methyltransferases (EC 2.1.1.37)
    Language English
    Publishing date 2018-04-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1474722-4
    ISSN 1476-4679 ; 1465-7392
    ISSN (online) 1476-4679
    ISSN 1465-7392
    DOI 10.1038/s41556-018-0087-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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