LIVIVO - Search results -

Search results

Result 1 - 10 of total 22

Search options

Article ; Online: Effects of blood sample storage time, temperature, anti-coagulants and blood stabiliser on lymphocyte phenotyping.

Lao, Yunyu / Quach, Alex / Perveen, Khalida / Hii, Charles / Ferrante, Antonio

2024 Volume 56, Issue 4, Page(s) 571–576

Abstract: Medical diagnostic laboratories have come under further scrutiny to ensure quality standards of their service and external quality assurance (EQA) programs involving multiple laboratories have been used to gauge this quality based on a consensus. However, ...

Abstract	Medical diagnostic laboratories have come under further scrutiny to ensure quality standards of their service and external quality assurance (EQA) programs involving multiple laboratories have been used to gauge this quality based on a consensus. However, because of the geographical distances within a country or internationally, cell surface marker expressions may change due to time delays and transport temperatures. Attention was given to this issue some decades ago and hence requires a re-evaluation in consideration of updated methods, reagents and instruments for flow cytometry and phenotyping. We have undertaken an extensive study to examine the effects of various conditions on blood storage akin to that experienced by patient samples as well as EQA programs, examining expression of lymphocyte surface markers, CD3, CD4, CD8, CD2, CD19, CD20, CD16/56 and HLA-DR. Assessment of lithium-heparin anticoagulated whole blood showed an increase in percentage of CD3
MeSH term(s)	Humans ; Temperature ; Immunophenotyping ; Flow Cytometry ; Anticoagulants/pharmacology ; Anticoagulants/therapeutic use ; Time Factors ; Lymphocytes ; Blood Preservation ; Blood Specimen Collection/methods ; Phenotype
Chemical Substances	Anticoagulants
Language	English
Publishing date	2024-02-07
Publishing country	England
Document type	Journal Article
ZDB-ID	7085-3
ISSN	1465-3931 ; 0031-3025
ISSN (online)	1465-3931
ISSN	0031-3025
DOI	10.1016/j.pathol.2023.11.011
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 723: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: The Application of Dextran Sedimentation as an Initial Step in Neutrophil Purification Promotes Their Stimulation, due to the Presence of Monocytes.

Quach, Alex / Ferrante, Antonio

Journal of immunology research

2017 Volume 2017, Page(s) 1254792

Abstract: The purification of human neutrophils ... ...

Abstract	The purification of human neutrophils for
MeSH term(s)	Blood Sedimentation ; CD11b Antigen/metabolism ; Cell Count ; Cell Separation/methods ; Cells, Cultured ; Centrifugation, Density Gradient ; Dextrans ; Diatrizoate ; Ficoll ; Fractionation, Field Flow/methods ; Humans ; L-Selectin/metabolism ; Neutrophil Activation ; Neutrophils/pathology
Chemical Substances	CD11b Antigen ; Dextrans ; Diatrizoate (117-96-4) ; L-Selectin (126880-86-2) ; Ficoll (25702-74-3)
Language	English
Publishing date	2017
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2817541-4
ISSN	2314-7156 ; 2314-8861
ISSN (online)	2314-7156
ISSN	2314-8861
DOI	10.1155/2017/1254792
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: PKCζ activation promotes maturation of cord blood T cells towards a Th1 IFN-γ propensity.

Perveen, Khalida / Quach, Alex / Stark, Michael J / Prescott, Susan / Barry, Simon C / Hii, Charles S / Ferrante, Antonio

Immunology

2023 Volume 170, Issue 3, Page(s) 359–373

Abstract: A significant number of babies present transiently with low protein kinase C zeta (PKCζ) levels in cord blood T cells (CBTC), associated with reduced ability to transition from a neonatal Th2 to a mature Th1 cytokine bias, leading to a higher risk of ... ...

Abstract	A significant number of babies present transiently with low protein kinase C zeta (PKCζ) levels in cord blood T cells (CBTC), associated with reduced ability to transition from a neonatal Th2 to a mature Th1 cytokine bias, leading to a higher risk of developing allergic sensitisation, compared to neonates whose T cells have 'normal' PKCζ levels. However, the importance of PKCζ signalling in regulating their differentiation from a Th2 to a Th1 cytokine phenotype propensity remains undefined. To define the role of PKCζ signalling in the regulation of CBTC differentiation from a Th2 to a Th1cytokine phenotype we have developed a neonatal T cell maturation model which enables the cells to develop to CD45RA
MeSH term(s)	Infant, Newborn ; Humans ; Interferon-gamma/metabolism ; Th1 Cells/metabolism ; Fetal Blood ; Cytokines/metabolism ; Cell Differentiation ; Leukocyte Common Antigens ; Th2 Cells/metabolism
Chemical Substances	Interferon-gamma (82115-62-6) ; Cytokines ; Leukocyte Common Antigens (EC 3.1.3.48)
Language	English
Publishing date	2023-06-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80124-0
ISSN	1365-2567 ; 0019-2805 ; 0953-4954
ISSN (online)	1365-2567
ISSN	0019-2805 ; 0953-4954
DOI	10.1111/imm.13674
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Ud II Zs.181: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Delayed Blood Processing Leads to Rapid Deterioration in the Measurement of the Neutrophil Respiratory Burst by the Dihydrorhodamine-123 Reduction Assay.

Quach, Alex / Glowik, Shannon / Putty, Trishni / Ferrante, Antonio

Cytometry. Part B, Clinical cytometry

2019 Volume 96, Issue 5, Page(s) 389–396

Abstract: Background: Neutrophils ex vivo in whole blood specimens are widely understood to decay rapidly when compared to other leukocytes, requiring assessment of neutrophil activity to be performed shortly after blood collection. There is a disparity in ... ...

Abstract	Background: Neutrophils ex vivo in whole blood specimens are widely understood to decay rapidly when compared to other leukocytes, requiring assessment of neutrophil activity to be performed shortly after blood collection. There is a disparity in evidence for decay rates in measurements and recommended time-frames for assaying neutrophil parameters in particular assays following blood collection. We, therefore, evaluated the decline in the neutrophil respiratory burst, typically screened for assessing congenital NADPH oxidase defects, over a shorter time-course than previously published experiments. Methods: The neutrophil respiratory burst was assessed by flow cytometric detection of DHR-123 oxidation to rhodamine-123 (Rho123), following stimulation of neutrophils by phorbol myristate acetate (PMA), in heparinized healthy donor blood specimens immediately following venipuncture, and then at 3 and 5 h later with ambient temperature or refrigerated specimen storage. Results: A consistent time-dependent decline in the Rho123 fluorescence of PMA-stimulated neutrophils was detected in the healthy donor specimens, indicating a decay in respiratory burst activity. Neutrophil oxidative indexes calculated for half of the specimens at 3 and 5 h of age, fell below our normal laboratory lower limit. We also found that Rho123 histograms of PMA-stimulated neutrophils from stored healthy donor specimens have a risk of misinterpretation due to mimicking the appearance of histograms from carriers of CGD and other NADPH oxidase defects. Refrigeration of specimens did not significantly minimize decay. Conclusions: DHR assay of the neutrophil respiratory burst from blood specimens at 3 h post-venipuncture and beyond can generate unreliable clinical measurements due to decay. © 2019 International Clinical Cytometry Society.
MeSH term(s)	Blood Chemical Analysis ; Blood Specimen Collection ; Flow Cytometry ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/metabolism ; Humans ; Neutrophils/chemistry ; Neutrophils/cytology ; Neutrophils/metabolism ; Oxidation-Reduction ; Respiratory Burst ; Rhodamine 123/chemistry ; Rhodamine 123/metabolism ; Rhodamines/chemistry ; Rhodamines/metabolism ; Temperature
Chemical Substances	Fluorescent Dyes ; Rhodamines ; dihydrorhodamine 123 (109244-58-8) ; Rhodamine 123 (1N3CZ14C5O)
Language	English
Publishing date	2019-02-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2099657-3
ISSN	1552-4957 ; 1552-4949 ; 0196-4763
ISSN (online)	1552-4957
ISSN	1552-4949 ; 0196-4763
DOI	10.1002/cyto.b.21767
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1688: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Neutrophils Require Activation to Express Functional Cell-Surface Complement Receptor Immunoglobulin.

Small, Annabelle G / Perveen, Khalida / Putty, Trishni / Patel, Nikita / Quinn, Patrick / Wechalekar, Mihir D / Hii, Charles S / Quach, Alex / Ferrante, Antonio

Frontiers in immunology

2022 Volume 13, Page(s) 840510

Abstract: The phagocytosis-promoting complement receptor, Complement Receptor Immunoglobulin (CRIg), is exclusively expressed on macrophages. It has been demonstrated that expression in macrophages could be modulated by inflammatory mediators, including cytokines. ...

Abstract	The phagocytosis-promoting complement receptor, Complement Receptor Immunoglobulin (CRIg), is exclusively expressed on macrophages. It has been demonstrated that expression in macrophages could be modulated by inflammatory mediators, including cytokines. This raised the possibility that a major phagocyte, the neutrophil, may also express CRIg following activation with inflammatory mediators. Here we show that resting peripheral blood neutrophil lysates subjected to protein analysis by Western blot revealed a 35 kDa CRIg isoform, consistent with the expression of CRIg mRNA by RT-PCR. By flow cytometry, CRIg was detected intracellularly and in very minor amounts on the cell surface. Interestingly, expression on the cell surface was significantly increased to functional levels after activation with inflammatory mediators/neutrophil activators; N-Formylmethionine-leucyl-phenylalanine, tumor necrosis factor (TNF), Granulocyte-Macrophage Colony stimulating Factor (GM-CSF), bacterial lipopolysaccharide, leukotriene B4 and phorbol myristate acetate. The increase in expression required p38 MAP kinase and protein kinase C activation, as well as intracellular calcium. Neutrophils which were defective in actin microfilament reorganization due to a mutation in ARPC1B or inhibition of its upstream regulator, Rac2 lose their ability to upregulate CRIg expression. Inhibition of another small GTPase, Rab27a, with pharmacological inhibitors prevented the increase in CRIg expression, suggesting a requirement for the actin cytoskeleton and exocytosis. Engagement of CRIg on TNF-primed neutrophils with an anti-CRIg monoclonal antibody increased the release of superoxide and promoted the activation of p38 but not ERK1/ERK2 or JNK MAP kinases. The TNF-induced increase in killing of
MeSH term(s)	Cytokines/metabolism ; Granulocyte-Macrophage Colony-Stimulating Factor/metabolism ; Immunoglobulins/metabolism ; Inflammation Mediators/metabolism ; Neutrophils/metabolism ; Receptors, Complement/genetics ; Receptors, Complement/metabolism ; Tumor Necrosis Factor-alpha/metabolism
Chemical Substances	Cytokines ; Immunoglobulins ; Inflammation Mediators ; Receptors, Complement ; Tumor Necrosis Factor-alpha ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1)
Language	English
Publishing date	2022-03-04
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.840510
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Book ; Online: Out of Distribution Generalization via Interventional Style Transfer in Single-Cell Microscopy

Pernice, Wolfgang M. / Doron, Michael / Quach, Alex / Pratapa, Aditya / Kenjeyev, Sultan / De Veaux, Nicholas / Hirano, Michio / Caicedo, Juan C.

2023

Abstract: Real-world deployment of computer vision systems, including in the discovery processes of biomedical research, requires causal representations that are invariant to contextual nuisances and generalize to new data. Leveraging the internal replicate ... ...

Abstract	Real-world deployment of computer vision systems, including in the discovery processes of biomedical research, requires causal representations that are invariant to contextual nuisances and generalize to new data. Leveraging the internal replicate structure of two novel single-cell fluorescent microscopy datasets, we propose generally applicable tests to assess the extent to which models learn causal representations across increasingly challenging levels of OOD-generalization. We show that despite seemingly strong performance, as assessed by other established metrics, both naive and contemporary baselines designed to ward against confounding, collapse on these tests. We introduce a new method, Interventional Style Transfer (IST), that substantially improves OOD generalization by generating interventional training distributions in which spurious correlations between biological causes and nuisances are mitigated. We publish our code and datasets. Comment: Accepted at CVPR 2023 CVMI
Keywords	Computer Science - Computer Vision and Pattern Recognition ; Computer Science - Artificial Intelligence ; Computer Science - Machine Learning
Subject code	006
Publishing date	2023-06-15
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Characterization of the Transient Deficiency of PKC Isozyme Levels in Immature Cord Blood T Cells and Its Connection to Anti-Allergic Cytokine Profiles of the Matured Cells.

Perveen, Khalida / Quach, Alex / Stark, Michael J / Prescott, Susan L / Barry, Simon C / Hii, Charles S / Ferrante, Antonio

International journal of molecular sciences

2021 Volume 22, Issue 23

Abstract: Cord blood T cells (CBTC) from a proportion of newborns express low/deficient levels of some protein kinase C (PKC) isozymes, with low levels of PKCζ correlating with increased risk of developing allergy and associated decrease in interferon-gamma (IFN-γ) ...

Abstract	Cord blood T cells (CBTC) from a proportion of newborns express low/deficient levels of some protein kinase C (PKC) isozymes, with low levels of PKCζ correlating with increased risk of developing allergy and associated decrease in interferon-gamma (IFN-γ) producing T cells. Interestingly, these lower levels of PKCζ were increased/normalized by supplementing women during pregnancy with n-3 polyunsaturated fatty acids. However, at present, we have little understanding of the transient nature of the deficiency in the neonate and how PKCζ relates to other PKC isozymes and whether their levels influence maturation into IFN-γ producing T cells. There is also no information on PKCζ isozyme levels in the T cell subpopulations, CD4
MeSH term(s)	CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Female ; Fetal Blood/cytology ; Fetal Blood/immunology ; Gestational Age ; Humans ; Infant, Newborn ; Interferon-gamma/metabolism ; Interleukin-10/metabolism ; Interleukin-2/metabolism ; Interleukin-9/metabolism ; Male ; Phytohemagglutinins/pharmacology ; Pregnancy ; Protein Kinase C/genetics ; Tumor Necrosis Factor-alpha/metabolism
Chemical Substances	IFNG protein, human ; IL10 protein, human ; IL2 protein, human ; IL9 protein, human ; Interleukin-2 ; Interleukin-9 ; Phytohemagglutinins ; TNF protein, human ; Tumor Necrosis Factor-alpha ; Interleukin-10 (130068-27-8) ; Interferon-gamma (82115-62-6) ; protein kinase C zeta (EC 2.7.11.1) ; Protein Kinase C (EC 2.7.11.13)
Language	English
Publishing date	2021-11-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms222312650
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively.

Perveen, Khalida / Quach, Alex / McPhee, Andrew / Prescott, Susan L / Barry, Simon C / Hii, Charles S / Ferrante, Antonio

International journal of molecular sciences

2021 Volume 22, Issue 9

Abstract: Low Protein Kinase C zeta (PKCζ) levels in cord blood T cells (CBTC) have been shown to correlate with the development of allergic sensitization in childhood. However, little is known about the mechanisms responsible. We have examined the relationship ... ...

Abstract	Low Protein Kinase C zeta (PKCζ) levels in cord blood T cells (CBTC) have been shown to correlate with the development of allergic sensitization in childhood. However, little is known about the mechanisms responsible. We have examined the relationship between the expression of different levels of PKCζ in CBTC and their development into mature T cell cytokine producers that relate to allergy or anti-allergy promoting cells. Maturation of naïve CBTC was initiated with anti-CD3/-CD28 antibodies and recombinant human interleukin-2 (rhIL-2). To stimulate lymphocyte proliferation and cytokine production the cells were treated with Phytohaemagglutinin (PHA) and Phorbol myristate acetate (PMA). Irrespective of the PKCζ levels expressed, immature CBTC showed no difference in lymphocyte proliferation and the production of T helper 2 (Th2) cytokine interleukin-4 (IL-4) and Th1 cytokine, interferon-gamma (IFN-γ), and influenced neither their maturation from CD45RA
MeSH term(s)	Apoptosis ; Cell Differentiation ; Cell Proliferation ; Cell Survival ; Cytokines ; Fetal Blood/cytology ; Humans ; Protein Kinase C/metabolism ; T-Lymphocytes/enzymology ; Th1 Cells/cytology ; Th1 Cells/metabolism ; Th2 Cells/cytology ; Th2 Cells/metabolism
Chemical Substances	Cytokines ; protein kinase C zeta (EC 2.7.11.1) ; Protein Kinase C (EC 2.7.11.13)
Language	English
Publishing date	2021-05-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22094907
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Measuring the Asian seabass (Lates calcarifer) neutrophil respiratory burst activity by the dihydrorhodamine-123 reduction flow cytometry assay in whole blood.

Hastuti, Sri D / Quach, Alex / Costabile, Maurizio / Barton, Mary D / Pyecroft, Stephen B / Ferrante, Antonio

Fish & shellfish immunology

2019 Volume 92, Page(s) 871–880

Abstract: The neutrophil oxidative respiratory burst response is a key component of the innate immune system responsible for killing microbial pathogens. Since fish rely on the innate immune system for health, monitoring the respiratory burst activity may be an ... ...

Abstract	The neutrophil oxidative respiratory burst response is a key component of the innate immune system responsible for killing microbial pathogens. Since fish rely on the innate immune system for health, monitoring the respiratory burst activity may be an effective means of gauging fish health status. Here we report that the respiratory burst of Asian seabass neutrophils can be measured in whole blood by the dihydrorhodamine (DHR)-123 reduction assay and flow cytometry. Neutrophils responded to phorbol myristate acetate (PMA) in a concentration dependent manner with significant respiratory burst activity at 100-1000 nM. Other known neutrophil agonists, such as bacterial lipopolysaccharide, tumor necrosis factor, the tripeptide f-met-leu-phe and zymosan, did not induce a significant DHR reduction. Thus, the findings enable us to propose that the DHR-123 flow cytometry whole blood assay, incorporating PMA as a stimulator, would not only facilitate future studies into fish blood neutrophil research but provides a simple, rapid and reliable assay for gauging fish natural immunity status and health.
MeSH term(s)	Animals ; Bass/physiology ; Flow Cytometry/methods ; Flow Cytometry/veterinary ; Immunity, Innate ; Neutrophils/physiology ; Oxidation-Reduction ; Respiratory Burst/physiology ; Rhodamines/chemistry
Chemical Substances	Rhodamines ; dihydrorhodamine 123 (109244-58-8)
Language	English
Publishing date	2019-07-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2019.07.018
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 5917: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Measuring the Asian seabass (Lates calcarifer) neutrophil respiratory burst activity by the dihydrorhodamine-123 reduction flow cytometry assay in whole blood

Hastuti, Sri D / Barton, Mary D / Costabile, Maurizio / Ferrante, Antonio / Pyecroft, Stephen B / Quach, Alex

Fish & shellfish immunology. 2019 Sept., v. 92

2019

Abstract	The neutrophil oxidative respiratory burst response is a key component of the innate immune system responsible for killing microbial pathogens. Since fish rely on the innate immune system for health, monitoring the respiratory burst activity may be an effective means of gauging fish health status. Here we report that the respiratory burst of Asian seabass neutrophils can be measured in whole blood by the dihydrorhodamine (DHR)-123 reduction assay and flow cytometry. Neutrophils responded to phorbol myristate acetate (PMA) in a concentration dependent manner with significant respiratory burst activity at 100–1000 nM. Other known neutrophil agonists, such as bacterial lipopolysaccharide, tumor necrosis factor, the tripeptide f-met-leu-phe and zymosan, did not induce a significant DHR reduction. Thus, the findings enable us to propose that the DHR-123 flow cytometry whole blood assay, incorporating PMA as a stimulator, would not only facilitate future studies into fish blood neutrophil research but provides a simple, rapid and reliable assay for gauging fish natural immunity status and health.
Keywords	acetates ; agonists ; fish ; fish health ; flow cytometry ; health status ; innate immunity ; Lates calcarifer ; lipopolysaccharides ; monitoring ; neutrophils ; pathogens ; respiratory burst ; tripeptides ; tumor necrosis factors ; zymosan
Language	English
Dates of publication	2019-09
Size	p. 871-880.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2019.07.018
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 5917: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED