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Article ; Online: Weathering-independent differentiation of microplastic polymers by reflectance IR spectrometry and pattern recognition.

Ferreiro, Borja / Andrade, Jose M / Paz-Quintáns, Carlota / Fernández-González, Verónica / López-Mahía, Purificación / Muniategui, Soledad

Marine pollution bulletin

2022 Volume 181, Page(s) 113897

Abstract: The presence and effects of microplastics in the environment is being continuously studied, so the need for a reliable approach to ascertain the polymer/s constituting them has increased. To characterize them, infrared (IR) spectrometry is commonly ... ...

Abstract	The presence and effects of microplastics in the environment is being continuously studied, so the need for a reliable approach to ascertain the polymer/s constituting them has increased. To characterize them, infrared (IR) spectrometry is commonly applied, either reflectance or attenuated total reflectance (ATR). A common problem when considering field samples is their weathering and biofouling, which modify their spectra. Hence, relying on spectral matching between the unknown spectrum and spectral databases is largely defective. In this paper, the use of IR spectra combined with pattern recognition techniques (principal components analysis, classification and regression trees and support vector classification) is explored first time to identify a collection of typical polymers regardless of their ageing. Results show that it is possible to identify them using a reduced suite of spectral wavenumbers with coherent chemical meaning. The models were validated using two datasets containing artificially weathered polymers and field samples.
MeSH term(s)	Microplastics ; Plastics/analysis ; Polymers ; Spectrophotometry, Infrared/methods ; Weather
Chemical Substances	Microplastics ; Plastics ; Polymers
Language	English
Publishing date	2022-07-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2001296-2
ISSN	1879-3363 ; 0025-326X
ISSN (online)	1879-3363
ISSN	0025-326X
DOI	10.1016/j.marpolbul.2022.113897
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Article: Clinical practice of embryo transfer.

Pasqualini, R S / Quintans, C J

Reproductive biomedicine online

2002 Volume 4, Issue 1, Page(s) 83–92

Abstract: In this review, several embryo transfer methods are considered, together with factors involved in achieving an effective transfer. The approach most used is transcervical intrauterine transfer. This is described in detail, together with the many ... ...

Abstract	In this review, several embryo transfer methods are considered, together with factors involved in achieving an effective transfer. The approach most used is transcervical intrauterine transfer. This is described in detail, together with the many variables influencing success, e.g. technical ability and training of personnel, catheter choice, value of a previous 'dummy transfer' and the need to minimize trauma during transfer and so prevent damage to the uterine lining, bleeding and uterine contractions. These factors can each negatively impact on pregnancy rates. Emphasis is put on quality, developmental stage and number of embryos to be transferred to limit multiple pregnancies and their unwanted side-effects. Culture to blastocyst stages and single embryo transfer when optimal quality embryos are available are discussed as means of avoiding multiple pregnancies. Reference is made to embryo cryopreservation and fertility following frozen embryo transfer. Other techniques, such as ultrasound-controlled transcervical intrauterine transfer, and ultrasound-controlled transmyometrial transfer, are reviewed. More invasive procedures, generically grouped as surgical embryo transfer, including gamete intra-Fallopian transfer (GIFT), zygote intra-Fallopian transfer (ZIFT), pronuclear stage transfer and embryo intra-Fallopian transfer (EIFT), are also described. These techniques had a place in IVF when the need to apply assisted reproductive techniques exceeded the capacity of most laboratories, but not today thanks to refined laboratory technology and improved understanding of implantation. Alternative assisted reproductive technologies, such as direct intra-follicular insemination (DIFI), Fallopian spermatic perfusion (FSP), peritoneal oocyte stage and sperm transfer and intra-vaginal culture (IVC), are mentioned briefly.
MeSH term(s)	Cryopreservation ; Embryo Transfer ; Female ; Fertility ; Fertilization in Vitro ; Humans ; Pregnancy ; Pregnancy, Multiple ; Reproductive Techniques, Assisted ; Ultrasonics
Language	English
Publishing date	2002-11-25
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	2113823-0
ISSN	1472-6491 ; 1472-6483
ISSN (online)	1472-6491
ISSN	1472-6483
DOI	10.1016/s1472-6483(10)61920-7
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Article: Birth resulting from transfer of blastocysts cryopreserved with propanediol after spontaneous hatching.

Quintans, C J / Donaldson, M J / Bertolino, M V / Pasqualini, R S

Reproductive biomedicine online

2003 Volume 6, Issue 1, Page(s) 66–68

Abstract: This paper reports the birth of a healthy baby resulting from transfer of blastocysts that were cryopreserved using propanediol after spontaneous hatching. A young infertile couple underwent IVF treatment in the clinic. After several IVF attempts, two ... ...

Abstract	This paper reports the birth of a healthy baby resulting from transfer of blastocysts that were cryopreserved using propanediol after spontaneous hatching. A young infertile couple underwent IVF treatment in the clinic. After several IVF attempts, two births resulted; the first one with fresh embryos in 1996 after three IVF cycles, and the second one in 1999 (after a new IVF cycle in 1998) with frozen blastocysts that had remained cryopreserved in 1.5 mol/l propanediol and 0.1 mol/l sucrose after spontaneous hatching. This report of a healthy baby following transfer of hatched blastocysts frozen in propanediol supports further exploration of this approach.
MeSH term(s)	Adult ; Blastocyst/metabolism ; Cryopreservation ; Embryo Transfer ; Female ; Humans ; Pregnancy ; Propylene Glycols/metabolism
Chemical Substances	Propylene Glycols
Language	English
Publishing date	2003-01-28
Publishing country	Netherlands
Document type	Case Reports ; Journal Article
ZDB-ID	2113823-0
ISSN	1472-6491 ; 1472-6483
ISSN (online)	1472-6491
ISSN	1472-6483
DOI	10.1016/s1472-6483(10)62057-3
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Article: Tratamiento con enoxaparina adaptado a los programas de fertilidad en mujeres con aborto recurrente y trombofilia.

Sarto, A / Rocha, M / Geller, M / Capmany, C / Martínez, M / Quintans, C / Donaldson, M / Pasqualini, R S

Medicina

2001 Volume 61, Issue 4, Page(s) 406–412

Abstract: Acquired and inherited thrombophilia are associated with recurrent pregnancy loss (RPL). Antithrombotic therapy could restore hemostatic balance and improve early placentation and gestational outcome. We evaluated the efficacy of enoxaparin adapted to ... ...

Title translation	Treatment with enoxaparin adapted to the fertility programs in women with recurrent abortion and thrombophilia.
Abstract	Acquired and inherited thrombophilia are associated with recurrent pregnancy loss (RPL). Antithrombotic therapy could restore hemostatic balance and improve early placentation and gestational outcome. We evaluated the efficacy of enoxaparin adapted to the fertility program for prevention of pregnancy loss in 35 women (W) with early RPL and thrombophilia. Previous to the diagnosis of thrombophilia, they had had a total of 105 gestations of which 89 (85%) ended in early pregnancy loss. After diagnosis of thrombophilia, 35 subsequent pregnancies were treated with enoxaparin. In 5 cases assisted reproductive techniques were necessary to achieve pregnancy due to couple infertility. In 17 W who had had at least one preclinical pregnancy loss, enoxaparin (20 mg/d/s.c.) was started previous to conception and adapted to the fertility program. All the women continued with the gestational regime. Eighteen W with only clinical pregnancy loss started enoxaparin (20 mg twice per day s.c.) after biochemical pregnancy diagnosis. During gestations heparin dose was adjusted with anti Xa test, maintaining a range between 0.3 at 0.6 u/ml. With antithrombotic therapy, 30/35 (85%) of the pregnancies ended in live birth versus 16/105 (15%) of the pregnancies without treatment (p < 0.001). These results suggest that enoxaparin adapted to the fertility program can be effective in the prevention of preclinical and clinical abortion in women with thrombophilia.
MeSH term(s)	Abortion, Habitual/etiology ; Abortion, Habitual/prevention & control ; Adult ; Biomarkers ; Embryo Loss/etiology ; Embryo Loss/prevention & control ; Enoxaparin/therapeutic use ; Female ; Fibrinolytic Agents/therapeutic use ; Heparin, Low-Molecular-Weight/therapeutic use ; Humans ; Infertility, Female/drug therapy ; Pregnancy ; Thrombophilia/complications ; Thrombophilia/diagnosis ; Thrombophilia/drug therapy
Chemical Substances	Biomarkers ; Enoxaparin ; Fibrinolytic Agents ; Heparin, Low-Molecular-Weight
Language	Spanish
Publishing date	2001
Publishing country	Argentina
Document type	English Abstract ; Journal Article
ZDB-ID	411586-7
ISSN	1669-9106 ; 0025-7680 ; 0325-951X
ISSN (online)	1669-9106
ISSN	0025-7680 ; 0325-951X
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Article: Transferencia nuclear y clonación.

Quintans, C J / Sergio Pasqualini, R

Medicina

1997 Volume 57, Issue 3, Page(s) 373–375

Title translation	Nuclear transfer and cloning.
MeSH term(s)	Animals ; Cell Nucleus ; Cloning, Organism/methods
Language	Spanish
Publishing date	1997
Publishing country	Argentina
Document type	Editorial
ZDB-ID	411586-7
ISSN	1669-9106 ; 0025-7680 ; 0325-951X
ISSN (online)	1669-9106
ISSN	0025-7680 ; 0325-951X
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Article: Embarazo con espermatozoides testiculares.

Sergio Pasqualini, R / Quintans, C / Valzacchi, G R / Matarasso, N / Martínez, M

Medicina

1995 Volume 55, Issue 3, Page(s) 249–251

Abstract: In a patient with a diagnosis of congenital vas deferens agenesis we decided to carry out a microsurgical epididymal sperm aspiration in order to attempt an in vitro fertilization (IVF). Since during scrotal exploration no epididymus was found (probably ... ...

Title translation	Pregnancy with testicular spermatozoa.
Abstract	In a patient with a diagnosis of congenital vas deferens agenesis we decided to carry out a microsurgical epididymal sperm aspiration in order to attempt an in vitro fertilization (IVF). Since during scrotal exploration no epididymus was found (probably as a consequence of a previous surgical biopsy) a testicle biopsy was performed. The latter yielded a few hundred sperms which were injected within the cytoplasm of his wife's oocytes (ICSI technique). Of the 18 oocytes, 3 were harmed during the procedure and 16 hours later signs of normal fertilization were detected in 6 of them, followed by the development of 4 embryos. These were transcervically transferred to the uterus, 3 in 8 cell stage and 1 in 6 cell stage; previously a 20 micron hole had been made with acid Tyrode solution in the pelucida zone of each embryo. Fifteen days later, the result of the HCG beta subunit assay was positive and a month later a single 19 mm normal embryo was detected by ultrasound echography.
MeSH term(s)	Adult ; Embryo Transfer ; Female ; Fertilization in Vitro/methods ; Humans ; Male ; Microinjections ; Micromanipulation ; Oligospermia ; Pregnancy ; Sperm-Ovum Interactions ; Spermatozoa ; Testis/cytology
Language	Spanish
Publishing date	1995
Publishing country	Argentina
Document type	Case Reports ; English Abstract ; Journal Article
ZDB-ID	411586-7
ISSN	1669-9106 ; 0025-7680 ; 0325-951X
ISSN (online)	1669-9106
ISSN	0025-7680 ; 0325-951X
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Article: Efecto de la suplementación dietética con acido gama-linolenico sobre el crecimiento de un carcinoma de pulmón humano implantado en ratones atímicos.

de Bravo, M G / Tournier, H / Schinella, G / Viaggi, M / Quintans, C

Medicina

1995 Volume 55, Issue 6, Page(s) 670–674

Abstract: We have studied the effect of a gamma-linolenic acid (18:3 n-6, GLA)-supplemented diet on the growth of a human lung mucoepidermoid carcinoma (HLMC) implanted in athymic mice and on its uptake of human low density lipoproteins labeled with 99mTc (99mTc- ... ...

Title translation	Effect of dietary supplementation with gamma-linolenic acid on the growth of a human lung carcinoma implanted in nude mice.
Abstract	We have studied the effect of a gamma-linolenic acid (18:3 n-6, GLA)-supplemented diet on the growth of a human lung mucoepidermoid carcinoma (HLMC) implanted in athymic mice and on its uptake of human low density lipoproteins labeled with 99mTc (99mTc-LDL). Mice bearing the HLMC were divided into two experimental groups. One of them was administered a control diet (C diet) and the other one was given a diet supplemented with 25 mg GLA/g pellet (GLA diet) for three weeks (Table 1). A tumor growth inhibition with the GLA diet was evident at the second week of treatment, and a marked inhibition (56%) was reached at the end of the third week (Fig. 1). The GLA diet produced some changes in the total fatty acid composition of tumor, plasma and liver of host mice: GLA and arachidonic acid (20:4 n-6, AA) induced significant increases, whereas oleic (18:1 n-9, OA) and linoleic acids (18:2 n-6, LA) were decreased (Table 2). Tumors of those animals fed both diets were labeled by 99mTc-LDL, and no difference was observed in the ratio of tumor/liver and tumor/kidney uptake of host animal (Table 3). Results obtained using this experimental model suggest that the inhibitory effect of GLA on tumor growth is not related to the LDL tumor uptake.
MeSH term(s)	Analysis of Variance ; Animals ; Carcinoma, Mucoepidermoid/pathology ; Diet ; Fatty Acids/chemistry ; Female ; Food, Fortified ; Humans ; Lipoproteins, LDL/blood ; Lung Neoplasms/pathology ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Organotechnetium Compounds ; gamma-Linolenic Acid/administration & dosage
Chemical Substances	Fatty Acids ; Lipoproteins, LDL ; Organotechnetium Compounds ; technetium Tc 99m low-density lipoprotein ; gamma-Linolenic Acid (78YC2MAX4O)
Language	Spanish
Publishing date	1995
Publishing country	Argentina
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	411586-7
ISSN	1669-9106 ; 0025-7680 ; 0325-951X
ISSN (online)	1669-9106
ISSN	0025-7680 ; 0325-951X
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Article: Calomys laucha (Rodentia, Cricetidae): growth and breeding in laboratory conditions.

Hodara, V L / Espinosa, M B / Merani, M S / Quintans, C

Laboratory animals

1989 Volume 23, Issue 4, Page(s) 340–344

Abstract: The husbandry and breeding of Calomys laucha (Rodentia, Cricetidae) in captivity are described. Growth curves based on body weight and length showed statistical differences between sexes after 45 days, males being heavier than females. The overall ... ...

Abstract	The husbandry and breeding of Calomys laucha (Rodentia, Cricetidae) in captivity are described. Growth curves based on body weight and length showed statistical differences between sexes after 45 days, males being heavier than females. The overall reproductive efficiency was 53.4% but birth rate was depressed during winter. Gestation length was 21 +/- 1 days and females exhibited postpartum oestrus with a 3-7 day implantation delay (51%). Litter size was 5.3 +/- 1.1 (n = 34). Pup survival at weaning was 84.9%. Mean life span in laboratory conditions was 13.5 months and a cumulative mortality of 90% was reached at 27-28 months of age.
MeSH term(s)	Animal Husbandry ; Animals ; Animals, Laboratory/growth & development ; Arvicolinae/growth & development ; Breeding ; Female ; Longevity ; Male ; Pregnancy ; Sex Factors
Language	English
Publishing date	1989-10
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391008-8
ISSN	1758-1117 ; 0023-6772
ISSN (online)	1758-1117
ISSN	0023-6772
DOI	10.1258/002367789780745962
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Article: Establecimiento de un banco de embriones congelados de ratón.

Montoro, L S / Quintans, C J

Medicina

1985 Volume 45, Issue 1, Page(s) 88–89

Title translation	Establishment of a frozen mouse embryo bank.
MeSH term(s)	Animals ; Embryo, Mammalian ; Freezing ; Mice ; Preservation, Biological
Language	Spanish
Publishing date	1985
Publishing country	Argentina
Document type	Letter
ZDB-ID	411586-7
ISSN	1669-9106 ; 0025-7680 ; 0325-951X
ISSN (online)	1669-9106
ISSN	0025-7680 ; 0325-951X
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Article: Empty follicle syndrome due to human errors: its occurrence in an in-vitro fertilization programme.

Quintans, C J / Donaldson, M J / Blanco, L A / Pasqualini, R S

Human reproduction (Oxford, England)

1998 Volume 13, Issue 1O, Page(s) 2703–2705

Abstract: We report five cases in which no oocytes were retrieved after standard ovarian stimulation for in-vitro fertilization (IVF), and in which it was found that mistakes had been made at the time of human chorionic gonadotrophin (HCG) administration. In all ... ...

Abstract	We report five cases in which no oocytes were retrieved after standard ovarian stimulation for in-vitro fertilization (IVF), and in which it was found that mistakes had been made at the time of human chorionic gonadotrophin (HCG) administration. In all five cases, oocyte retrieval was achieved after injecting HCG, when necessary, and reprogramming aspiration 24-36 h later. A mean of 7+/-3.2 MII oocytes were recovered per patient and 3.2+/-0.8 embryos were transferred. Three clinical pregnancies were obtained, and four healthy infants were born. In our programme, these were the only cases of empty follicle syndrome (EFS) that appeared over a total of 1118 cycles, and were all explained by human error in the administration of HCG. Our experience shows that human error could be considered a significant factor in the aetiology of empty follicle syndrome, and that EFS may be in part avoided by taking simple preventive measures.
MeSH term(s)	Adult ; Chorionic Gonadotropin/administration & dosage ; Chorionic Gonadotropin/adverse effects ; Embryo Transfer ; Female ; Fertilization in Vitro/adverse effects ; Fertilization in Vitro/methods ; Humans ; Infant, Newborn ; Inhalation ; Male ; Oocytes/cytology ; Ovarian Follicle/cytology ; Ovulation Induction/adverse effects ; Ovulation Induction/methods ; Pregnancy ; Pregnancy Outcome ; Syndrome ; Time Factors
Chemical Substances	Chorionic Gonadotropin
Language	English
Publishing date	1998-10
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	632776-x
ISSN	1460-2350 ; 0268-1161 ; 1477-741X
ISSN (online)	1460-2350
ISSN	0268-1161 ; 1477-741X
DOI	10.1093/humrep/13.10.2703
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