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  1. Article ; Online: Synthesis and characterization of graphene oxide (GO) sheets for pathogen inhibition

    Leda G. Bousiakou / Rabia Qindeel / Omar M. Al-Dossary / Helen Kalkani

    Journal of King Saud University: Science, Vol 34, Iss 4, Pp 102002- (2022)

    Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa

    2022  

    Abstract: It is well known that graphene oxide (GO) possesses antibacterial properties towards both Gram-positive and Gram-negative bacteria. As a result, it has recently attracted a lot of interest as an active component towards pathogen inhibition. The aim of ... ...

    Abstract It is well known that graphene oxide (GO) possesses antibacterial properties towards both Gram-positive and Gram-negative bacteria. As a result, it has recently attracted a lot of interest as an active component towards pathogen inhibition. The aim of this paper is to prepare graphene oxide sheets, with two different oxygen concentrations of 15% and 39%, characterize them, and then investigate in vitro their antimicrobial efficacy in both Gram-positive and Gram-negative bacteria. In particular, GO sheets are synthesized via the Hummer’s method and subsequently, electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy are used to investigate the GO sheet morphology and structure, while Energy Dispersive X-ray Spectroscopy (EDS) is employed for elemental analysis. Subsequently, clinical isolates of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa are incubated with different concentrations of GO sheets, ranging from 0.25 mg/ml to 1.5 mg/ml for 24 h at 37 °C to evaluate the antimicrobial effect. The results confirmed that that Gram positive bacteria are more sensitive to GO, while GO with a higher oxygen content exhibits increased antibacterial activity.
    Keywords Graphene oxide ; GO ; Hummer method ; Antibacterial activity ; Escherichia coli ; Staphylococcus aureus ; Science (General) ; Q1-390
    Subject code 540
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Atomic Structure Calculations for Neutral Oxygen

    Norah Alonizan / Rabia Qindeel / Nabil Ben Nessib

    International Journal of Spectroscopy, Vol

    2016  Volume 2016

    Abstract: Energy levels and oscillator strengths for neutral oxygen have been calculated using the Cowan (CW), SUPERSTRUCTURE (SS), and AUTOSTRUCTURE (AS) atomic structure codes. The results obtained with these atomic codes have been compared with MCHF ... ...

    Abstract Energy levels and oscillator strengths for neutral oxygen have been calculated using the Cowan (CW), SUPERSTRUCTURE (SS), and AUTOSTRUCTURE (AS) atomic structure codes. The results obtained with these atomic codes have been compared with MCHF calculations and experimental values from the National Institute of Standards and Technology (NIST) database.
    Keywords Optics. Light ; QC350-467 ; Chemistry ; QD1-999
    Language English
    Publishing date 2016-01-01T00:00:00Z
    Publisher Hindawi Publishing Corporation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Green synthesis of Moringa oleifera leaf nanoparticles and an assessment of their therapeutic potential

    Promy Virk / Manal A. Awad / Sarah Saleh Abdu-llah Alsaif / Awatif A. Hendi / Mai Elobeid / Khalid Ortashi / Rabia Qindeel / Manal F. El-Khadragy / Hany M. Yehia / Mohamed Ferkry Serag EL-DIN / Hatem Ali Salama

    Journal of King Saud University: Science, Vol 35, Iss 3, Pp 102576- (2023)

    2023  

    Abstract: The present study centered around the prospect of bio fabricating nanoparticles using Moringa oleifera leaf and investigating their therapeutic potential with an assessment of the antioxidant status. Synthesis of stable ‘green’ nanoparticles of Moringa ... ...

    Abstract The present study centered around the prospect of bio fabricating nanoparticles using Moringa oleifera leaf and investigating their therapeutic potential with an assessment of the antioxidant status. Synthesis of stable ‘green’ nanoparticles of Moringa leaf extract is successfully reported with an extensive characterization. The average particle size and polydispersity index (PDI) of the nanoparticles obtained by Dynamic light scattering (DLS) analysis were 141.6 nm and a polydispersity index (PDI) of 0.32 respectively. The UV–visible spectroscopy (UV–vis) revealed a strong red absorbance peak at 664 nm,characteristic of chlorophyll. Electron micrographs confirmed that particles were in the nano range and were spherical with minimal agglomeration. The constituent functional groups were assessed by Fourier transformed infrared spectroscopy (FTIR). The X-ray diffractograms of reflected both the amorphous and crystalline domains.Furthermore, the phytochemical screening showed a higher phenolic/ flavonoid content of the Moringa nanoparticles (NMo) in comparison to the bulk Moringa leaf extract(Mo). These results corresponded to a potent antioxidant status of the NMo which was assessed by three complementary antioxidant assays. The therapeutic potential of the nanoparticles was evaluated based on in vitro anti-diabetic assays and cytotoxicity assay on human cancer cell lines (MCF-7 and HepG-2). The key findings suggest a more profound therapeutic efficacy of NMo than the Moringa leaf extract.
    Keywords Moringa oleifera ; Green synthesis ; Antioxidant ; Phytochemical profile ; Anti-diabetic ; Anticancer ; Science (General) ; Q1-390
    Subject code 500
    Language English
    Publishing date 2023-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Protective effect of probiotic bacteria and its nanoformulation against cadmium-induced oxidative stress in male Wistar rat

    Amenah Mohmmad Monadi Al-Enazi / Promy Virk / Awatif Hindi / Manal. A. Awad / Mai Elobeid / Rabia Qindeel

    Journal of King Saud University: Science, Vol 32, Iss 7, Pp 3045-

    2020  Volume 3051

    Abstract: The present study assessed the ameliorative potential of a commercial probiotic, Protexin® and its nanoformulation against cadmium (Cd) toxicity in Wistar rats. The ‘green’ synthesized nanoparticles had a size of 170 nm (PDI 0.527) with spherical and ... ...

    Abstract The present study assessed the ameliorative potential of a commercial probiotic, Protexin® and its nanoformulation against cadmium (Cd) toxicity in Wistar rats. The ‘green’ synthesized nanoparticles had a size of 170 nm (PDI 0.527) with spherical and tubular bacillary shapes. Rats were exposed to 70 ppm of cadmium chloride hydrate for 35 days. Group I and V were negative and positive control respectively. Groups II, III, and IV were exposed to Cd. The Cd exposed groups, III and IV were treated with the probiotic and nanoprobiotic (1 ml containing 2 × 108cfu) respectively. Cd intoxication led to significant bioaccumulation of cadmium in liver and kidney. A significant increase in the serum malondialdehyde (MDA) and 8 -hydroxydeoxyguanosine (8-OHdG) levels was observed. The metallothionien (MT) and reduced glutathione (GSH) levels in the liver were significantly increased. The advance oxidation protein products (AOPP) and methylglyoxal (MG) levels were also elevated. Treatment with probiotic and its nanoparticles markedly reversed the Cd induced alterations in the parameters. The nanoformulation was more profound in reducing the (GSH) and MT levels in the liver and 8-OHdG levels in the serum. The key findings suggest that a nutritional intervention in the form of probiotic supplementation is a safe and efficacious remediation against heavy metal toxicity/oxidative stress.
    Keywords Cadmium ; Probiotics ; Nanoparticles ; Oxidative stress ; Science (General) ; Q1-390
    Subject code 500
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Assessing the Effectiveness of Microelement Removal in the South Tertiary Wastewater Plant, Riyadh KSA

    Leda G. Bousiakou / Rabia Qindeel / A. S. Almuzaini / Hosham A. Alghamdi / Walid Tawfik / W. A Farooq / H. Kalkani / E. Manzou

    Current World Environment, Vol 10, Iss 3, Pp 772-

    2015  Volume 780

    Abstract: This work focuses on the monitoring of trace element removal from the Riyadh South Tertiary Wastewater Treatment plant using inductively coupled plasma mass spectrometry (ICP-MS). Considering that the final effluent originating from the plant is directed ...

    Abstract This work focuses on the monitoring of trace element removal from the Riyadh South Tertiary Wastewater Treatment plant using inductively coupled plasma mass spectrometry (ICP-MS). Considering that the final effluent originating from the plant is directed for irrigation purposes towards the farms of Al- Dirayia, Dirab and Wadi Hanifa it is important to consider the possible presence of elevated microelement concentrations that could pose potential threats to the human health. All samples were collected from the initial entrance to the plant representing the raw influent as well as the final exit after chlorination, i.e. the final effluent used for irrigation purposes. Results showed that the concentration of aluminium (Al), phosphorus (P), copper (Cu), manganese (Mn) and lead (Pb) were initially elevated at their entrance to the plant compared to the recommended values by the Environmental Protection Agency (EPA) and the World Health Organisation (WHO), while zinc (Zn), chromium (Cr), molybdenum (Mo), selenium (Se), cobalt (Co), uranium ( U), mercury (Hg), arsenic (As), and cadmium (Cd) were within permissible levels. All microelements showed significant reduction of concentration with values well below the maximum recommendations. The observed results are important for assessing the functions and effectiveness of the treatment methods of the plant as well as ensuring that the final effluent is appropriate for agricultural use.
    Keywords wastewater ; trace elements ; ICP-MS ; heavy metals ; irrigation ; chlorination ; Environmental sciences ; GE1-350 ; Geography. Anthropology. Recreation ; G
    Subject code 660
    Language English
    Publishing date 2015-12-01T00:00:00Z
    Publisher Enviro Research Publishers
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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