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  1. Article: Redox-Modified Nanostructured Electrochemical Surfaces for Continuous Glucose Monitoring in Complex Biological Fluids.

    Janfaza, Sajjad / Radha Shanmugam, Nandhinee / Jolly, Pawan / Kovur, Prashanthi / Singh, Upasana / Mackay, Scott / Wishart, David / Ingber, Donald E

    Nanomaterials (Basel, Switzerland)

    2024  Volume 14, Issue 9

    Abstract: Continuous glucose monitoring is valuable for people with diabetes but faces limitations due to enzyme-electrode interactions and biofouling from biological samples that reduce sensor sensitivity and the monitoring performance. We created an enzyme-based ...

    Abstract Continuous glucose monitoring is valuable for people with diabetes but faces limitations due to enzyme-electrode interactions and biofouling from biological samples that reduce sensor sensitivity and the monitoring performance. We created an enzyme-based electrochemical system with a unique nanocomposite coating that incorporates the redox molecule, aminoferrocene (NH
    Language English
    Publishing date 2024-05-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano14090796
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The Anatomy of a Nonfaradaic Electrochemical Biosensor.

    Stevenson, Hunter / Radha Shanmugam, Nandhinee / Paneer Selvam, Anjan / Prasad, Shalini

    SLAS technology

    2017  Volume 23, Issue 1, Page(s) 5–15

    Abstract: Point-of-care (POC) testing has revolutionized diagnostic healthcare, bringing medical results directly and immediately to the patient. With faster diagnostics, more immediate clinical management decisions can be made. POC tests most often use a dipstick ...

    Abstract Point-of-care (POC) testing has revolutionized diagnostic healthcare, bringing medical results directly and immediately to the patient. With faster diagnostics, more immediate clinical management decisions can be made. POC tests most often use a dipstick or swab format to detect the presence of a pathogen, disease, or other relevant biomarker. In these formats, the POC tests eliminate the need for complex lab equipment and trained personnel to collect, process, and analyze sample data for simple diagnostics. However, these tests cannot satisfy all clinical needs, because accurate quantitative results are needed. The present study serves as a template for designing a nonfaradaic electrochemical biosensor toward quantitative POC diagnostics. We focus on investigating the most important parameters when constructing a nonfaradaic biosensor through both mathematical modeling and electrochemical measurements. Furthermore, we demonstrate quantitative affinity biosensing of a model protein toward developing a POC device.
    MeSH term(s) Biomarkers/analysis ; Biosensing Techniques/methods ; Diagnostic Tests, Routine/methods ; Electrochemical Techniques/methods ; Humans ; Models, Theoretical ; Point-of-Care Systems ; Proteins/analysis
    Chemical Substances Biomarkers ; Proteins
    Language English
    Publishing date 2017-11-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2900310-6
    ISSN 2472-6311 ; 2472-6303
    ISSN (online) 2472-6311
    ISSN 2472-6303
    DOI 10.1177/2472630317738700
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Ultrasensitive nanostructure sensor arrays on flexible substrates for multiplexed and simultaneous electrochemical detection of a panel of cardiac biomarkers

    Radha Shanmugam, Nandhinee / Jonathan Anguiano / Shajee Chaudhry / Shalini Prasad / Sriram Muthukumar

    Biosensors & bioelectronics. 2017 Mar. 15, v. 89

    2017  

    Abstract: Multiplexed detection of protein biomarkers offers new opportunities for early diagnosis and efficient treatment of complex diseases. Cardiovascular diseases (CVDs) has the highest mortality risk in USA and Europe with 15–20 million cases being reported ... ...

    Abstract Multiplexed detection of protein biomarkers offers new opportunities for early diagnosis and efficient treatment of complex diseases. Cardiovascular diseases (CVDs) has the highest mortality risk in USA and Europe with 15–20 million cases being reported annually. Cardiac Troponins (T and I) are well established protein biomarkers associated with heart muscle damage and point-of-care monitoring of both these two biomarkers has significant benefits on patient care. A flexible disposable electrochemical biosensor device comprising of vertically oriented zinc oxide (ZnO) nanostructures was developed for rapid and simultaneous screening of cardiac Troponin-I (cTnI) and cardiac-Troponin-T (cTnT) in a point-of-care sensor format. The biosensors were designed by selective hydrothermal growth of ZnO nanostructures onto the working electrodes of polyimide printed circuit board platforms, resulting in the generation of high density nanostructure ZnO arrays based electrodes. The size, density and surface terminations of the nanostructures were leveraged towards achieving surface confinement of the target cTnT and cTnI molecules on to the electrode surface. Multiplexing and simultaneous detection was achieved through sensor platform design comprising of arrays of Troponin functionalized ZnO nanostructure electrodes. The sensitivity and specificity of the biosensor was characterized using two types of electrochemical techniques; electrochemical impedance spectroscopy (EIS) and Mott-Schottky analysis on the same sensor platform to demonstrate multi-configurable modes. Limit of detection of 1pg/mL in human serum was achieved for both cTnI and cTnT. Cross reactivity analysis showed the selectivity of detecting cTnT and cTnI in human serum with wide dynamic range.
    Keywords biomarkers ; biosensors ; blood serum ; cardiovascular diseases ; cross reaction ; detection limit ; dielectric spectroscopy ; early diagnosis ; electrochemistry ; electrodes ; heart ; humans ; monitoring ; mortality ; muscles ; nanomaterials ; patient care ; risk ; screening ; troponin I ; troponin T ; zinc oxide ; Europe ; United States
    Language English
    Dates of publication 2017-0315
    Size p. 764-772.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2016.10.046
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Ultrasensitive nanostructure sensor arrays on flexible substrates for multiplexed and simultaneous electrochemical detection of a panel of cardiac biomarkers.

    Radha Shanmugam, Nandhinee / Muthukumar, Sriram / Chaudhry, Shajee / Anguiano, Jonathan / Prasad, Shalini

    Biosensors & bioelectronics

    2017  Volume 89, Issue Pt 2, Page(s) 764–772

    Abstract: Multiplexed detection of protein biomarkers offers new opportunities for early diagnosis and efficient treatment of complex diseases. Cardiovascular diseases (CVDs) has the highest mortality risk in USA and Europe with 15-20 million cases being reported ... ...

    Abstract Multiplexed detection of protein biomarkers offers new opportunities for early diagnosis and efficient treatment of complex diseases. Cardiovascular diseases (CVDs) has the highest mortality risk in USA and Europe with 15-20 million cases being reported annually. Cardiac Troponins (T and I) are well established protein biomarkers associated with heart muscle damage and point-of-care monitoring of both these two biomarkers has significant benefits on patient care. A flexible disposable electrochemical biosensor device comprising of vertically oriented zinc oxide (ZnO) nanostructures was developed for rapid and simultaneous screening of cardiac Troponin-I (cTnI) and cardiac-Troponin-T (cTnT) in a point-of-care sensor format. The biosensors were designed by selective hydrothermal growth of ZnO nanostructures onto the working electrodes of polyimide printed circuit board platforms, resulting in the generation of high density nanostructure ZnO arrays based electrodes. The size, density and surface terminations of the nanostructures were leveraged towards achieving surface confinement of the target cTnT and cTnI molecules on to the electrode surface. Multiplexing and simultaneous detection was achieved through sensor platform design comprising of arrays of Troponin functionalized ZnO nanostructure electrodes. The sensitivity and specificity of the biosensor was characterized using two types of electrochemical techniques; electrochemical impedance spectroscopy (EIS) and Mott-Schottky analysis on the same sensor platform to demonstrate multi-configurable modes. Limit of detection of 1pg/mL in human serum was achieved for both cTnI and cTnT. Cross reactivity analysis showed the selectivity of detecting cTnT and cTnI in human serum with wide dynamic range.
    MeSH term(s) Antibodies, Immobilized/chemistry ; Biomarkers/blood ; Biosensing Techniques/instrumentation ; Cardiovascular Diseases/blood ; Electrochemical Techniques/instrumentation ; Equipment Design ; Humans ; Immunoassay/instrumentation ; Limit of Detection ; Nanostructures/chemistry ; Nanostructures/ultrastructure ; Point-of-Care Systems ; Troponin I/blood ; Troponin T/blood ; Zinc Oxide/chemistry
    Chemical Substances Antibodies, Immobilized ; Biomarkers ; Troponin I ; Troponin T ; Zinc Oxide (SOI2LOH54Z)
    Language English
    Publishing date 2017-03-15
    Publishing country England
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2016.10.046
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Flexible Molybdenum Electrodes towards Designing Affinity Based Protein Biosensors.

    Kamakoti, Vikramshankar / Panneer Selvam, Anjan / Radha Shanmugam, Nandhinee / Muthukumar, Sriram / Prasad, Shalini

    Biosensors

    2016  Volume 6, Issue 3

    Abstract: Molybdenum electrode based flexible biosensor on porous polyamide substrates has been fabricated and tested for its functionality as a protein affinity based biosensor. The biosensor performance was evaluated using a key cardiac biomarker; cardiac ... ...

    Abstract Molybdenum electrode based flexible biosensor on porous polyamide substrates has been fabricated and tested for its functionality as a protein affinity based biosensor. The biosensor performance was evaluated using a key cardiac biomarker; cardiac Troponin-I (cTnI). Molybdenum is a transition metal and demonstrates electrochemical behavior upon interaction with an electrolyte. We have leveraged this property of molybdenum for designing an affinity based biosensor using electrochemical impedance spectroscopy. We have evaluated the feasibility of detection of cTnI in phosphate-buffered saline (PBS) and human serum (HS) by measuring impedance changes over a frequency window from 100 mHz to 1 MHz. Increasing changes to the measured impedance was correlated to the increased dose of cTnI molecules binding to the cTnI antibody functionalized molybdenum surface. We achieved cTnI detection limit of 10 pg/mL in PBS and 1 ng/mL in HS medium. The use of flexible substrates for designing the biosensor demonstrates promise for integration with a large-scale batch manufacturing process.
    MeSH term(s) Antibodies ; Biosensing Techniques/methods ; Biosensing Techniques/standards ; Dielectric Spectroscopy ; Electrodes ; Immunoassay/methods ; Immunoassay/standards ; Molybdenum ; Proteins ; Troponin I
    Chemical Substances Antibodies ; Proteins ; Troponin I ; Molybdenum (81AH48963U)
    Language English
    Publishing date 2016-07-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios6030036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Novel Nanomonitor ultra-sensitive detection of troponin T.

    Barrett, Thomas W / Radha Shanmugam, Nandhinee / Selvam, Anjan Panneer / Kazmierczak, Steven C / Prasad, Shalini

    Clinica chimica acta; international journal of clinical chemistry

    2015  Volume 442, Page(s) 96–101

    Abstract: Background: Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses ... ...

    Abstract Background: Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker.
    Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method.
    Results: The limit of detection of the Nanomonior for troponin T was 0.0088ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73ng/l and 1800ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r=1.00, and an intercept of 15.88ng/l (95% confidence interval, -68.39ng/l, 100.15ng/l). The mean difference between the assays was -7.54ng/l, determined by Bland-Altman analysis.
    Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15min, and are portable. More research is needed.
    MeSH term(s) Blood Chemical Analysis/methods ; Humans ; Limit of Detection ; Nanotechnology/methods ; Troponin T/blood
    Chemical Substances Troponin T
    Language English
    Publishing date 2015-03-10
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2015.01.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.173: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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