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  1. Article ; Online: First evidence of free-living Naegleria species in recreational lakes of Alberta, Canada

    Rafik Dey / Elena Dlusskaya / Mariem Oloroso / Nicholas J. Ashbolt

    Journal of Water and Health, Vol 21, Iss 3, Pp 439-

    2023  Volume 442

    Abstract: Rising temperatures are increasing environmental habitats for thermotolerant pathogens, such as the so-called ‘brain-eating amoeba’, Naegleria fowleri. To the best of our knowledge, however, Naegleria species have not been reported in environmental water ...

    Abstract Rising temperatures are increasing environmental habitats for thermotolerant pathogens, such as the so-called ‘brain-eating amoeba’, Naegleria fowleri. To the best of our knowledge, however, Naegleria species have not been reported in environmental water sources in Canada. We surveyed popular recreational lakes in Alberta, Canada during the summer bathing period to determine the presence or absence of Naegleria species. While N. fowleri was not isolated in this study, we identified other thermotolerant species, including Naegleria pagei, Naegleria gruberi, Naegleria jejuensis and Naegleria fultoni using culture-based methods, hence indicating the potential conditions to support N. fowleri. Ongoing monitoring and examination of water for pathogenic amoebae is recommended in order to assist in the public health management of water sources. HIGHLIGHTS Survey of Naegleria species in recreational lakes in Alberta, Canada.; First evidence of thermotolerant Naegleria species detection in Canada.; Climate change and northern expansion of Naegleria species.;
    Keywords amoebae ; canadian lakes ; climate change ; legionella ; naegleria spp ; waterborne pathogens ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher IWA Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae

    Rafik Dey / Elena Dlusskaya / Nicholas J. Ashbolt

    Journal of Water and Health, Vol 20, Iss 1, Pp 83-

    2022  Volume 91

    Abstract: The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying ... ...

    Abstract The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying the interaction of the enveloped bacteriophage Phi6, as a potential surrogated along with one of the most common amoebae in engineered aquatic environments, Vermamoeba vermiformis. Using microscopy, imaging flow cytometry and bacteriophage cell culture, our results imply that the SARS-CoV-2 surrogate triggers amoebic mitochondria and induced apoptosis to promote viral persistence in trophozoites. Furthermore, virus-infected amoebae were still infectious after 2 months within FLA cysts. These results suggest that amoebae could contribute to the environmental persistence of SARS-CoV-2, including disinfection processes. In addition, amoebae could be a successful model system for understanding respiratory virus-eukaryotic biology at the cellular and molecular levels. HIGHLIGHTS Amoebic mitochondria serve as a support organelle platform for the SARS-CoV-2 surrogate persistence.; Long-term stability of RNA enveloped viruses in amoeba-forming cysts.; Free-living amoebae as a potential vector and environmental reservoir for RNA enveloped viruses.;
    Keywords apoptosis ; bacteriophage phi6 ; environmental persistence ; free-living amoebae ; mitochondria ; sars-cov-2 ; transmission ; Public aspects of medicine ; RA1-1270
    Subject code 333
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher IWA Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Extracellular amoebal-vesicles

    Rafik Dey / Melanie A. Folkins / Nicholas J. Ashbolt

    npj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-

    potential transmission vehicles for respiratory viruses

    2021  Volume 5

    Abstract: Abstract Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of ... ...

    Abstract Abstract Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of infectious RSV that persisted for the duration of the experiment. Given the ubiquity of amoebae in moist environments, our results suggest that extracellular amoebal-vesicles could contribute to the environmental persistence of respiratory viruses, including potential resistance to disinfection processes and thereby offering novel pathways for viral dissemination and transmission.
    Keywords Microbial ecology ; QR100-130
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada

    Rafik Dey / Harley Mount / Alex W. Ensminger / Greg J. Tyrrell / Linda P. Ward / Nicholas J. Ashbolt

    Emerging Infectious Diseases, Vol 25, Iss 11, Pp 2104-

    2019  Volume 2107

    Abstract: Legionellosis was diagnosed in an immunocompromised 3-year-old girl in Canada. We traced the source of the bacterium through co-culture with an ameba collected from a hot tub in her home. We identified Legionella pneumophila serogroup 6, sequence type ... ...

    Abstract Legionellosis was diagnosed in an immunocompromised 3-year-old girl in Canada. We traced the source of the bacterium through co-culture with an ameba collected from a hot tub in her home. We identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm the environmental and clinical isolates were of common origin.
    Keywords Legionellosis ; case report ; hot tub ; Legionella pneumophila ; ameba ; coculture ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
    Language English
    Publishing date 2019-11-01T00:00:00Z
    Publisher Centers for Disease Control and Prevention
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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