Article ; Online: An integrated stage-tip-based glycomic and glycoproteomic approach for simple and rapid N-glycosylation profiling of glycoproteins.
Biomedical chromatography : BMC
2022 Volume 36, Issue 12, Page(s) e5503
Abstract: Glycosylation is a post-translational modification that plays an active role in many cellular events. It also regulates many functions of proteins. Monoclonal antibody-derived drugs are used to treat many diseases, and glycosylation affects the activity ... ...
| Abstract | Glycosylation is a post-translational modification that plays an active role in many cellular events. It also regulates many functions of proteins. Monoclonal antibody-derived drugs are used to treat many diseases, and glycosylation affects the activity of such drugs developed. On the other hand, N-glycans may change in certain diseases. Therefore, rapid and efficient bioanalytical methods are needed for N-glycosylation profiling. The study aimed to develop an integrated stage-tip application for the simple and rapid N-glycosylation profiling of glycoproteins. A fast and inexpensive N-glycosylation profiling was achieved by integrating all glycoproteomic and glycomic sample preparation steps into a stage-tip. The glycomic approach of the integrated stage-tip reduces the N-glycan profiling time from 2 days to approximately 2.5 hours. It also allows the profiling of immunoglobulin G (IgG) N-glycopeptides directly from human plasma. In addition, N-glycosylation profiling can be done in the developed method without sorbents, C |
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| MeSH term(s) | Humans ; Glycosylation ; Glycomics/methods ; Glycoproteins/metabolism ; Glycopeptides ; Polysaccharides | |||||
| Chemical Substances | Glycoproteins ; Glycopeptides ; Polysaccharides | |||||
| Language | English | |||||
| Publishing date | 2022-09-21 | |||||
| Publishing country | England | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 632848-9 | |||||
| ISSN | 1099-0801 ; 0269-3879 | |||||
| ISSN (online) | 1099-0801 | |||||
| ISSN | 0269-3879 | |||||
| DOI | 10.1002/bmc.5503 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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