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  1. Book: Calcium signaling protocols

    Lambert, David G. / Rainbow, Richard D.

    (Methods in molecular biology ; 937 ; Springer protocols)

    2013  

    Author's details ed. by David G. Lambert ; Richard D. Rainbow
    Series title Methods in molecular biology ; 937
    Springer protocols
    Collection
    Keywords Calcium / pharmacokinetics ; Calcium Signaling / physiology ; Signal Transduction ; Calcium Channels / pharmacokinetics ; Spectrometry, Fluorescence / methods ; Calcium--Laboratory manuals ; Calcium--Research--Methodology ; Calcium channels--Research--Methodology ; Cellular signal transduction--Research--Methodology
    Subject code 571.6
    Language English
    Size XI, 360 S. : Ill., graph. Darst., 27 cm
    Edition 3. ed.
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    Note Includes bibliographical references and index
    HBZ-ID HT017541287
    ISBN 978-1-62703-085-4 ; 1-62703-085-9 ; 9781627030861 ; 1627030867
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2013 A 400 available for loan (reading room) Lesesaal EG

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  2. Article ; Online: Mitigation of Cardiovascular Disease and Toxicity through NRF2 Signalling.

    Roberts, James A / Rainbow, Richard D / Sharma, Parveen

    International journal of molecular sciences

    2023  Volume 24, Issue 7

    Abstract: Cardiovascular toxicity and diseases are phenomena that have a vastly detrimental impact on morbidity and mortality. The pathophysiology driving the development of these conditions is multifactorial but commonly includes the perturbance of reactive ... ...

    Abstract Cardiovascular toxicity and diseases are phenomena that have a vastly detrimental impact on morbidity and mortality. The pathophysiology driving the development of these conditions is multifactorial but commonly includes the perturbance of reactive oxygen species (ROS) signalling, iron homeostasis and mitochondrial bioenergetics. The transcription factor nuclear factor erythroid 2 (NFE2)-related factor 2 (NRF2), a master regulator of cytoprotective responses, drives the expression of genes that provide resistance to oxidative, electrophilic and xenobiotic stresses. Recent research has suggested that stimulation of the NRF2 signalling pathway can alleviate cardiotoxicity and hallmarks of cardiovascular disease progression. However, dysregulation of NRF2 dynamic responses can be severely impacted by ageing processes and off-target toxicity from clinical medicines including anthracycline chemotherapeutics, rendering cells of the cardiovascular system susceptible to toxicity and subsequent tissue dysfunction. This review addresses the current understanding of NRF2 mechanisms under homeostatic and cardiovascular pathophysiological conditions within the context of wider implications for this diverse transcription factor.
    MeSH term(s) Humans ; Cardiovascular Diseases/metabolism ; Oxidative Stress/physiology ; NF-E2-Related Factor 2/genetics ; NF-E2-Related Factor 2/metabolism ; Antioxidants/metabolism ; Cardiovascular System/metabolism
    Chemical Substances NF-E2-Related Factor 2 ; Antioxidants
    Language English
    Publishing date 2023-04-04
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24076723
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Editorial: Insulin resistance and cardiovascular disease.

    Stewart, Alan J / Tuncay, Erkan / Pitt, Samantha J / Rainbow, Richard D

    Frontiers in endocrinology

    2023  Volume 14, Page(s) 1266173

    MeSH term(s) Humans ; Cardiovascular Diseases ; Insulin Resistance
    Language English
    Publishing date 2023-08-04
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1266173
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Combined calcium fluorescence recording with ionic currents in contractile cells.

    Rainbow, Richard D

    Methods in molecular biology (Clifton, N.J.)

    2013  Volume 937, Page(s) 149–160

    Abstract: Measurement of calcium (Ca(2+)) fluorescence in conjunction with ionic currents is of particular importance in contractile cells, such as cardiac ventricular myocytes and vascular smooth muscle. The interplay between membrane potential and intracellular ... ...

    Abstract Measurement of calcium (Ca(2+)) fluorescence in conjunction with ionic currents is of particular importance in contractile cells, such as cardiac ventricular myocytes and vascular smooth muscle. The interplay between membrane potential and intracellular calcium ([Ca(2+)](i)) is fundamental to the regulation of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with "free" indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.
    MeSH term(s) Animals ; Calcium/metabolism ; Cells, Cultured ; Electrophysiology/methods ; Fluorescence ; Guinea Pigs ; Ion Transport/physiology ; Male
    Chemical Substances Calcium (SY7Q814VUP)
    Language English
    Publishing date 2013
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-62703-086-1_8
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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  5. Article ; Online: Slowly activating voltage-gated potassium current potentiation by ML277 is a novel cardioprotective intervention.

    Brennan, Sean / Alnaimi, Abrar I M / McGuinness, Lauren R / Abdelaziz, Muhammad I M / McKenzie, Robert A / Draycott, Sophie / Whitmore, Jacob / Sharma, Parveen / Rainbow, Richard D

    PNAS nexus

    2023  Volume 2, Issue 5, Page(s) pgad156

    Abstract: Cardiovascular disease is thought to account for nearly a third of deaths worldwide, with ischemic heart disease, including acute coronary syndromes such as myocardial infarction, accounting for 1.7 million deaths per year. There is a clear need for ... ...

    Abstract Cardiovascular disease is thought to account for nearly a third of deaths worldwide, with ischemic heart disease, including acute coronary syndromes such as myocardial infarction, accounting for 1.7 million deaths per year. There is a clear need for interventions to impart cardioprotection against ischemia. Here, we show that the slowly activating voltage-gated potassium current (IKs) potentiator ML277 imparts cardioprotection against ischemia in cellular and whole-heart models by modulating the action potential duration. In three different metabolic inhibition and reperfusion models, an increased contractile recovery and cell survival was observed with ML277, indicative of protection. Finally, ML277 reduced infarct size in an ex vivo Langendorff coronary ligation model, including if only applied on reperfusion. In conclusion, potentiation of the IKs with ML277 imparted a cardioprotection that was equivalent to the protection reported previously by ischemic preconditioning. These data suggest that IKs potentiation may be therapeutically useful in acute coronary syndromes.
    Language English
    Publishing date 2023-05-10
    Publishing country England
    Document type Journal Article
    ISSN 2752-6542
    ISSN (online) 2752-6542
    DOI 10.1093/pnasnexus/pgad156
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book: Calcium signaling protocols

    Lambert, David G / Rainbow, Richard D

    (Methods in molecular biology, ; 937 ; Springer protocols,)

    2013  

    Author's details edited by David G. Lambert, Richard D. Rainbow
    Series title Methods in molecular biology, ; 937
    Springer protocols,
    MeSH term(s) Calcium/pharmacokinetics ; Calcium Signaling/physiology ; Signal Transduction ; Calcium Channels/pharmacokinetics ; Fluorometry/methods
    Language English
    Size xi, 360 p. :, ill. (some col.) ;, 24 cm.
    Edition 3rd ed.
    Publisher Humana
    Publishing place New York
    Document type Book
    ISBN 9781627030854 ; 1627030859 ; 9781627030861 ; 1627030867
    Database Catalogue of the US National Library of Medicine (NLM)

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Book: Calcium signaling protocols

    Lambert, David G / Rainbow, Richard D

    (Methods in molecular biology ; 937 ; Springer protocols)

    2013  

    Author's details ed. by David G. Lambert; Richard D. Rainbow
    Series title Methods in molecular biology ; 937
    Springer protocols
    Language English
    Size XI, 360 S.
    Edition 3. ed.
    Publisher Humana Press
    Publishing place New York, NJ u.a.
    Document type Book
    ISBN 9781627030854 ; 9781627030861 ; 1627030859 ; 1627030867
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  8. Article: Selective protein kinase C inhibition switches time-dependent glucose cardiotoxicity to cardioprotection.

    Brennan, Sean / Esposito, Simona / Abdelaziz, Muhammad I M / Martin, Christopher A / Makwana, Samir / Sims, Mark W / Squire, Iain B / Sharma, Parveen / Chadwick, Amy E / Rainbow, Richard D

    Frontiers in cardiovascular medicine

    2022  Volume 9, Page(s) 997013

    Abstract: Hyperglycaemia at the time of myocardial infarction has an adverse effect on prognosis irrespective of a prior diagnosis of diabetes, suggesting glucose is the damaging factor. ... ...

    Abstract Hyperglycaemia at the time of myocardial infarction has an adverse effect on prognosis irrespective of a prior diagnosis of diabetes, suggesting glucose is the damaging factor. In
    Language English
    Publishing date 2022-09-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2781496-8
    ISSN 2297-055X
    ISSN 2297-055X
    DOI 10.3389/fcvm.2022.997013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Deep-Channel uses deep neural networks to detect single-molecule events from patch-clamp data.

    Celik, Numan / O'Brien, Fiona / Brennan, Sean / Rainbow, Richard D / Dart, Caroline / Zheng, Yalin / Coenen, Frans / Barrett-Jolley, Richard

    Communications biology

    2020  Volume 3, Issue 1, Page(s) 3

    Abstract: Single-molecule research techniques such as patch-clamp electrophysiology deliver unique biological insight by capturing the movement of individual proteins in real time, unobscured by whole-cell ensemble averaging. The critical first step in analysis is ...

    Abstract Single-molecule research techniques such as patch-clamp electrophysiology deliver unique biological insight by capturing the movement of individual proteins in real time, unobscured by whole-cell ensemble averaging. The critical first step in analysis is event detection, so called "idealisation", where noisy raw data are turned into discrete records of protein movement. To date there have been practical limitations in patch-clamp data idealisation; high quality idealisation is typically laborious and becomes infeasible and subjective with complex biological data containing many distinct native single-ion channel proteins gating simultaneously. Here, we show a deep learning model based on convolutional neural networks and long short-term memory architecture can automatically idealise complex single molecule activity more accurately and faster than traditional methods. There are no parameters to set; baseline, channel amplitude or numbers of channels for example. We believe this approach could revolutionise the unsupervised automatic detection of single-molecule transition events in the future.
    MeSH term(s) Artificial Intelligence ; Electrophysiological Phenomena ; Humans ; Ion Channel Gating ; Ion Channels/metabolism ; Models, Biological ; Neural Networks, Computer ; Patch-Clamp Techniques ; ROC Curve ; Single Molecule Imaging/methods ; Supervised Machine Learning ; Workflow
    Chemical Substances Ion Channels
    Language English
    Publishing date 2020-01-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2399-3642
    ISSN (online) 2399-3642
    DOI 10.1038/s42003-019-0729-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Kir6.2-D323 and SUR2A-Q1336: an intersubunit interaction pairing for allosteric information transfer in the KATP channel complex.

    Brennan, Sean / Rubaiy, Hussein N / Imanzadeh, Saba / Reid, Ruth / Lodwick, David / Norman, Robert I / Rainbow, Richard D

    The Biochemical journal

    2020  Volume 477, Issue 3, Page(s) 671–689

    Abstract: ATP-sensitive potassium (KATP) channels are widely expressed and play key roles in many tissues by coupling metabolic state to membrane excitability. The SUR subunits confer drug and enhanced nucleotide sensitivity to the pore-forming Kir6 subunit, and ... ...

    Abstract ATP-sensitive potassium (KATP) channels are widely expressed and play key roles in many tissues by coupling metabolic state to membrane excitability. The SUR subunits confer drug and enhanced nucleotide sensitivity to the pore-forming Kir6 subunit, and so information transfer between the subunits must occur. In our previous study, we identified an electrostatic interaction between Kir6 and SUR2 subunits that was key for allosteric information transfer between the regulatory and pore-forming subunit. In this study, we demonstrate a second putative interaction between Kir6.2-D323 and SUR2A-Q1336 using patch clamp electrophysiological recording, where charge swap mutation of the residues on either side of the potential interaction compromise normal channel function. The Kir6.2-D323K mutation gave rise to a constitutively active, glibenclamide and ATP-insensitive KATP complex, further confirming the importance of information transfer between the Kir6 and SUR2 subunits. Sensitivity to modulators was restored when Kir6.2-D323K was co-expressed with a reciprocal charge swap mutant, SUR-Q1336E. Importantly, equivalent interactions have been identified in both Kir6.1 and Kir6.2 suggesting this is a second important interaction between Kir6 and the proximal C terminus of SUR.
    MeSH term(s) ATP-Binding Cassette Transporters/chemistry ; ATP-Binding Cassette Transporters/metabolism ; Allosteric Site ; HEK293 Cells ; Humans ; KATP Channels/chemistry ; KATP Channels/metabolism ; Models, Structural ; Mutation ; Patch-Clamp Techniques ; Potassium Channels, Inwardly Rectifying/chemistry ; Potassium Channels, Inwardly Rectifying/genetics ; Potassium Channels, Inwardly Rectifying/metabolism ; Sulfonylurea Receptors/chemistry ; Sulfonylurea Receptors/genetics ; Sulfonylurea Receptors/metabolism
    Chemical Substances ATP-Binding Cassette Transporters ; KATP Channels ; Kir6.2 channel ; Potassium Channels, Inwardly Rectifying ; Sulfonylurea Receptors
    Language English
    Publishing date 2020-01-20
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2969-5
    ISSN 1470-8728 ; 0006-2936 ; 0306-3275 ; 0264-6021
    ISSN (online) 1470-8728
    ISSN 0006-2936 ; 0306-3275 ; 0264-6021
    DOI 10.1042/BCJ20190753
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.110: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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