LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Rajanbabu, Ram"
  2. AU=Yilmaz Mahmut
  3. AU="Fathimathu, Zuhara"
  4. AU="Barbara I Kazmierczak"
  5. AU=Gendron Joshua M
  6. AU="Klaaßen-Mielke, Renate"
  7. AU=Rao Saieesh A AU=Rao Saieesh A
  8. AU="Md. Arifur Rahman Munshi"
  9. AU=Gorbunova Vera
  10. AU="Mathias Uhlen"
  11. AU="Torrens, Alexa"
  12. AU="Moloney, E"
  13. AU="Pulawski, S."
  14. AU="Ustuner, Mehmet Cengiz"
  15. AU="Solozhentseva, Kristina"
  16. AU="Sitar, Cristian"
  17. AU="Ahmed, Md Firoz"
  18. AU=Goldberg Elad
  19. AU="Moskowitz, Jeremy D"
  20. AU="Mugunthan, Susithra Priyadarshni"
  21. AU="Matini, Lawrence"
  22. AU="Pourova, Radka"
  23. AU="Saxena, Shweta"
  24. AU="McGovern, Sophie"
  25. AU="Shuai An"
  26. AU="Kirill S. Golokhvast"
  27. AU="Cho, Kwang-Hwi"
  28. AU="Davitoiu, Dragos"
  29. AU=Templeman Charles
  30. AU="Attaluri, Anilchandra"
  31. AU="Cox, Ryan M"
  32. AU="Barua, Melissa"
  33. AU=Wong Ngai-Sze
  34. AU="Salgotra, Romesh Kumar"
  35. AU="Rossano, Adam J"
  36. AU="Pfeiffer, Christian"
  37. AU="Klostermann, Cynthia E."
  38. AU="Ivory, Joannie M"
  39. AU="Sooltangos, Aisha"
  40. AU="Marcia Adriana Poll"
  41. AU="Wenzel, Ross"
  42. AU="Wang, Ruihan"
  43. AU=Qing Enya AU=Qing Enya
  44. AU=Xu Jian AU=Xu Jian

Suchergebnis

Treffer 1 - 3 von insgesamt 3

Suchoptionen

  1. Artikel ; Online: Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization.

    Lee, Brian R / Budzillo, Agata / Hadley, Kristen / Miller, Jeremy A / Jarsky, Tim / Baker, Katherine / Hill, DiJon / Kim, Lisa / Mann, Rusty / Ng, Lindsay / Oldre, Aaron / Rajanbabu, Ram / Trinh, Jessica / Vargas, Sara / Braun, Thomas / Dalley, Rachel A / Gouwens, Nathan W / Kalmbach, Brian E / Kim, Tae Kyung /
    Smith, Kimberly A / Soler-Llavina, Gilberto / Sorensen, Staci / Tasic, Bosiljka / Ting, Jonathan T / Lein, Ed / Zeng, Hongkui / Murphy, Gabe J / Berg, Jim

    eLife

    2021  Band 10

    Abstract: The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons. To generate Patch-seq datasets at scale, we ... ...

    Abstract The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons. To generate Patch-seq datasets at scale, we identified and refined key factors that contribute to the efficient collection of high-quality data. We developed patch-clamp electrophysiology software with analysis functions specifically designed to automate acquisition with online quality control. We recognized the importance of extracting the nucleus for transcriptomic success and maximizing membrane integrity during nucleus extraction for morphology success. The protocol is generalizable to different species and brain regions, as demonstrated by capturing multimodal data from human and macaque brain slices. The protocol, analysis and acquisition software are compiled at https://githubcom/AllenInstitute/patchseqtools. This resource can be used by individual labs to generate data across diverse mammalian species and that is compatible with large publicly available Patch-seq datasets.
    Mesh-Begriff(e) Animals ; Brain ; Electrophysiological Phenomena ; Humans ; Macaca mulatta ; Mice ; Neurons/cytology ; Neurons/physiology ; Patch-Clamp Techniques ; Single-Cell Analysis/methods ; Software ; Transcriptome
    Sprache Englisch
    Erscheinungsdatum 2021-08-13
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.65482
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel: Connecting single-cell transcriptomes to projectomes in mouse visual cortex.

    Sorensen, Staci A / Gouwens, Nathan W / Wang, Yun / Mallory, Matt / Budzillo, Agata / Dalley, Rachel / Lee, Brian / Gliko, Olga / Kuo, Hsien-Chi / Kuang, Xiuli / Mann, Rusty / Ahmadinia, Leila / Alfiler, Lauren / Baftizadeh, Fahimeh / Baker, Katherine / Bannick, Sarah / Bertagnolli, Darren / Bickley, Kris / Bohn, Phil /
    Brown, Dillan / Bomben, Jasmine / Brouner, Krissy / Chen, Chao / Chen, Kai / Chvilicek, Maggie / Collman, Forrest / Daigle, Tanya / Dawes, Tim / de Frates, Rebecca / Dee, Nick / DePartee, Maxwell / Egdorf, Tom / El-Hifnawi, Laila / Enstrom, Rachel / Esposito, Luke / Farrell, Colin / Gala, Rohan / Glomb, Andrew / Gamlin, Clare / Gary, Amanda / Goldy, Jeff / Gu, Hong / Hadley, Kristen / Hawrylycz, Mike / Henry, Alex / Hill, Dijon / Hirokawa, Karla E / Huang, Zili / Johnson, Katelyn / Juneau, Zoe / Kebede, Sara / Kim, Lisa / Lee, Changkyu / Lesnar, Phil / Li, Anan / Li, Yaoyao / Liang, Elizabeth / Link, Katie / Maxwell, Michelle / McGraw, Medea / McMillen, Delissa A / Mukora, Alice / Ng, Lindsay / Ochoa, Thomas / Oldre, Aaron / Park, Daniel / Pom, Christina Alice / Popovich, Zoran / Potekhina, Lydia / Rajanbabu, Ram / Ransford, Shea / Reding, Melissa / Ruiz, Augustin / Sandman, David / Siverts, La'Akea / Smith, Kimberly A / Stoecklin, Michelle / Sulc, Josef / Tieu, Michael / Ting, Jonathan / Trinh, Jessica / Vargas, Sara / Vumbaco, Dave / Walker, Miranda / Wang, Micheal / Wanner, Adrian / Waters, Jack / Williams, Grace / Wilson, Julia / Xiong, Wei / Lein, Ed / Berg, Jim / Kalmbach, Brian / Yao, Shenqin / Gong, Hui / Luo, Qingming / Ng, Lydia / Sümbül, Uygar / Jarsky, Tim / Yao, Zizhen / Tasic, Bosiljka / Zeng, Hongkui

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The mammalian brain is composed of diverse neuron types that play different functional roles. Recent single-cell RNA sequencing approaches have led to a whole brain taxonomy of transcriptomically-defined cell types, yet cell type definitions that include ...

    Abstract The mammalian brain is composed of diverse neuron types that play different functional roles. Recent single-cell RNA sequencing approaches have led to a whole brain taxonomy of transcriptomically-defined cell types, yet cell type definitions that include multiple cellular properties can offer additional insights into a neuron's role in brain circuits. While the Patch-seq method can investigate how transcriptomic properties relate to the local morphological and electrophysiological properties of cell types, linking transcriptomic identities to long-range projections is a major unresolved challenge. To address this, we collected coordinated Patch-seq and whole brain morphology data sets of excitatory neurons in mouse visual cortex. From the Patch-seq data, we defined 16 integrated morpho-electric-transcriptomic (MET)-types; in parallel, we reconstructed the complete morphologies of 300 neurons. We unified the two data sets with a multi-step classifier, to integrate cell type assignments and interrogate cross-modality relationships. We find that transcriptomic variations within and across MET-types correspond with morphological and electrophysiological phenotypes. In addition, this variation, along with the anatomical location of the cell, can be used to predict the projection targets of individual neurons. We also shed new light on infragranular cell types and circuits, including cell-type-specific, interhemispheric projections. With this approach, we establish a comprehensive, integrated taxonomy of excitatory neuron types in mouse visual cortex and create a system for integrated, high-dimensional cell type classification that can be extended to the whole brain and potentially across species.
    Sprache Englisch
    Erscheinungsdatum 2023-11-26
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.11.25.568393
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  3. Artikel ; Online: Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex.

    Lee, Brian R / Dalley, Rachel / Miller, Jeremy A / Chartrand, Thomas / Close, Jennie / Mann, Rusty / Mukora, Alice / Ng, Lindsay / Alfiler, Lauren / Baker, Katherine / Bertagnolli, Darren / Brouner, Krissy / Casper, Tamara / Csajbok, Eva / Donadio, Nicholas / Driessens, Stan L W / Egdorf, Tom / Enstrom, Rachel / Galakhova, Anna A /
    Gary, Amanda / Gelfand, Emily / Goldy, Jeff / Hadley, Kristen / Heistek, Tim S / Hill, Dijon / Hou, Wen-Hsien / Johansen, Nelson / Jorstad, Nik / Kim, Lisa / Kocsis, Agnes Katalin / Kruse, Lauren / Kunst, Michael / León, Gabriela / Long, Brian / Mallory, Matthew / Maxwell, Michelle / McGraw, Medea / McMillen, Delissa / Melief, Erica J / Molnar, Gabor / Mortrud, Marty T / Newman, Dakota / Nyhus, Julie / Opitz-Araya, Ximena / Ozsvár, Attila / Pham, Trangthanh / Pom, Alice / Potekhina, Lydia / Rajanbabu, Ram / Ruiz, Augustin / Sunkin, Susan M / Szöts, Ildikó / Taskin, Naz / Thyagarajan, Bargavi / Tieu, Michael / Trinh, Jessica / Vargas, Sara / Vumbaco, David / Waleboer, Femke / Walling-Bell, Sarah / Weed, Natalie / Williams, Grace / Wilson, Julia / Yao, Shenqin / Zhou, Thomas / Barzó, Pál / Bakken, Trygve / Cobbs, Charles / Dee, Nick / Ellenbogen, Richard G / Esposito, Luke / Ferreira, Manuel / Gouwens, Nathan W / Grannan, Benjamin / Gwinn, Ryder P / Hauptman, Jason S / Hodge, Rebecca / Jarsky, Tim / Keene, C Dirk / Ko, Andrew L / Korshoej, Anders Rosendal / Levi, Boaz P / Meier, Kaare / Ojemann, Jeffrey G / Patel, Anoop / Ruzevick, Jacob / Silbergeld, Daniel L / Smith, Kimberly / Sørensen, Jens Christian / Waters, Jack / Zeng, Hongkui / Berg, Jim / Capogna, Marco / Goriounova, Natalia A / Kalmbach, Brian / de Kock, Christiaan P J / Mansvelder, Huib D / Sorensen, Staci A / Tamas, Gabor / Lein, Ed S / Ting, Jonathan T

    Science (New York, N.Y.)

    2023  Band 382, Heft 6667, Seite(n) eadf6484

    Abstract: Human cortex transcriptomic studies have revealed a hierarchical organization of γ-aminobutyric acid-producing (GABAergic) neurons from subclasses to a high diversity of more granular types. Rapid GABAergic neuron viral genetic labeling plus Patch-seq ( ... ...

    Abstract Human cortex transcriptomic studies have revealed a hierarchical organization of γ-aminobutyric acid-producing (GABAergic) neurons from subclasses to a high diversity of more granular types. Rapid GABAergic neuron viral genetic labeling plus Patch-seq (patch-clamp electrophysiology plus single-cell RNA sequencing) sampling in human brain slices was used to reliably target and analyze GABAergic neuron subclasses and individual transcriptomic types. This characterization elucidated transitions between PVALB and SST subclasses, revealed morphological heterogeneity within an abundant transcriptomic type, identified multiple spatially distinct types of the primate-specialized double bouquet cells (DBCs), and shed light on cellular differences between homologous mouse and human neocortical GABAergic neuron types. These results highlight the importance of multimodal phenotypic characterization for refinement of emerging transcriptomic cell type taxonomies and for understanding conserved and specialized cellular properties of human brain cell types.
    Mesh-Begriff(e) Animals ; Humans ; Mice ; Electrophysiological Phenomena ; GABAergic Neurons/metabolism ; gamma-Aminobutyric Acid/metabolism ; Interneurons/metabolism ; Neocortex/cytology ; Neocortex/metabolism ; Patch-Clamp Techniques
    Chemische Substanzen gamma-Aminobutyric Acid (56-12-2)
    Sprache Englisch
    Erscheinungsdatum 2023-10-13
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.adf6484
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 27: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 77: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang