LIVIVO - Search results -

Search results

Result 1 - 9 of total 9

Search options

Article ; Online: Identification of prosthetic joint infections with 16S amplicon metagenomic sequencing - comparison with standard cultivation approach.

Mahnic, Aleksander / Rak, Mitja / Trebše, Rihard / Rupnik, Maja / Cőr, Andrej

Diagnostic microbiology and infectious disease

2024 Volume 109, Issue 1, Page(s) 116188

Abstract: Prosthetic joint infections (PJIs) are commonly diagnosed via culture-based methods, which may miss hard-to-grow pathogens. This study contrasts amplicon metagenomic sequencing (16S AS) with traditional culture techniques for enhanced clinical decision- ... ...

Abstract	Prosthetic joint infections (PJIs) are commonly diagnosed via culture-based methods, which may miss hard-to-grow pathogens. This study contrasts amplicon metagenomic sequencing (16S AS) with traditional culture techniques for enhanced clinical decision-making. We analyzed sonicate fluid from 27 patients undergoing revision arthroplasty using both methods, emphasizing the distinction between contaminants and true positives. Our findings show moderate agreement between the two methods, with a Cohen's kappa of 0.490, varying across bacterial genera (Cohen's kappa -0.059 to 1). The sensitivity of 16S AS compared to culture was 81% (95% CI, 68% to 94%). Sequencing revealed greater microbial diversity, including anaerobic genera like Anaerococcus and Citrobacter. Interestingly, several culture-negative PJI samples showed diverse bacteria via 16S AS. Despite rigorous controls and algorithms to eliminate contaminants, confirming bacteria presence with 16S AS remains a challenge. This highlights the need for improved PJI diagnostic methods, while also pointing out the limitations of next-generation sequencing (NGS) as a clinical diagnostic tool.
MeSH term(s)	Humans ; Arthritis, Infectious/diagnosis ; Bacteria/genetics ; Prostheses and Implants ; Arthroplasty ; High-Throughput Nucleotide Sequencing ; Prosthesis-Related Infections/diagnosis ; Prosthesis-Related Infections/microbiology ; Sensitivity and Specificity ; RNA, Ribosomal, 16S/genetics
Chemical Substances	RNA, Ribosomal, 16S
Language	English
Publishing date	2024-01-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	604920-5
ISSN	1879-0070 ; 0732-8893
ISSN (online)	1879-0070
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2024.116188
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1845: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Accuracy of Allplex SARS-CoV-2 assay amplification curve analysis for the detection of SARS-CoV-2 variant Alpha.

Čretnik, Tjaša Ž / Retelj, Matjaž / Janežič, Sandra / Mahnič, Aleksander / Tesovnik, Tine / Šket, Robert / Bizjan, Barbara J / Jeverica, Samo / Ravnik, Mateja / Rak, Mitja / Borinc, Mateja / Rupnik, Maja / Battelino, Tadej / Pokorn, Marko / Kovač, Jernej

Future microbiology

2022 Volume 17, Page(s) 1125–1131

Abstract: ... Aim: ... To evaluate the accuracy of two PCR-based techniques for detecting SARS-CoV-2 variant Alpha (B.1.1.7). ... Materials & methods: ... A multicenter prospective cohort with 1137 positive specimens from Slovenia was studied. A mutation-based assay ...

Abstract	Aim: To evaluate the accuracy of two PCR-based techniques for detecting SARS-CoV-2 variant Alpha (B.1.1.7). Materials & methods: A multicenter prospective cohort with 1137 positive specimens from Slovenia was studied. A mutation-based assay (rTEST-COVID-19 qPCR B.1.1.7 assay) and amplification curve pattern analysis of the Allplex SARS-CoV-2 assay were compared with whole-genome sequencing. Results: SARS-CoV-2 variant Alpha was detected in 155 samples (13.6%). Sensitivity and specificity were 98.1 and 98.0%, respectively, for the rTEST-COVID-19 qPCR B.1.1.7 assay and 97.4 and 97.5%, respectively, for amplification curve pattern analysis. Conclusion: The good analytical performance of both methods was confirmed for the preliminary identification of SARS-CoV-2 variant Alpha. This cost-effective principle for screening SARS-CoV-2 populations is also applicable to other emerging variants and may help to conserve some whole-genome sequencing resources.
MeSH term(s)	COVID-19/diagnosis ; Humans ; Prospective Studies ; SARS-CoV-2/genetics ; Sensitivity and Specificity
Language	English
Publishing date	2022-07-26
Publishing country	England
Document type	Journal Article ; Multicenter Study
ZDB-ID	2254620-0
ISSN	1746-0921 ; 1746-0913
ISSN (online)	1746-0921
ISSN	1746-0913
DOI	10.2217/fmb-2021-0288
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Detection of bacteria with molecular methods in prosthetic joint infection: sonication fluid better than periprosthetic tissue.

Rak, Mitja / KavčIč, Martina / Trebše, Rihard / CőR, Andrej

Acta orthopaedica

2016 Volume 87, Issue 4, Page(s) 339–345

Abstract: Background and purpose - The correct diagnosis of prosthetic joint infection (PJI) can be difficult because bacteria form a biofilm on the surface of the implant. The sensitivity of culture from sonication fluid is better than that from periprosthetic ... ...

Abstract	Background and purpose - The correct diagnosis of prosthetic joint infection (PJI) can be difficult because bacteria form a biofilm on the surface of the implant. The sensitivity of culture from sonication fluid is better than that from periprosthetic tissue, but no comparison studies using molecular methods on a large scale have been performed. We assessed whether periprosthetic tissue or sonication fluid should be used for molecular analysis. Patients and methods - Implant and tissue samples were retrieved from 87 patients who underwent revision operation of total knee or total hip arthroplasty. Both sample types were analyzed using broad-range (BR-) PCR targeting the 16S rRNA gene. The results were evaluated based on the definition of periprosthetic joint infection from the Workgroup of the Musculoskeletal Infection Society. Results - PJI was diagnosed in 29 patients, whereas aseptic failure was diagnosed in 58 patients. Analysis of sonication fluid using BR-PCR detected bacteria in 27 patients, whereas analysis of periprosthetic tissue by BR-PCR detected bacteria in 22 patients. In 6 of 7 patients in whom BR-PCR analysis of periprosthetic tissue was negative, low-virulence bacteria were present. The sensitivity and specificity values for periprosthetic tissue were 76% and 93%, respectively, and the sensitivity and specificity values for sonication fluid were 95% and 97%. Interpretation - Our results suggest that sonication fluid may be a more appropriate sample than periprosthetic tissue for BR-PCR analysis in patients with PJI. However, further investigation is required to improve detection of bacteria in patients with so-called aseptic failure.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Arthroplasty, Replacement, Hip/adverse effects ; Bacteria/genetics ; Bacteria/isolation & purification ; Bacteriological Techniques/methods ; DNA, Bacterial/analysis ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; Prospective Studies ; Prosthesis-Related Infections/diagnosis ; Prosthesis-Related Infections/microbiology ; Reoperation ; Sonication/methods
Chemical Substances	DNA, Bacterial
Language	English
Publishing date	2016-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2180677-9
ISSN	1745-3682 ; 1745-3674
ISSN (online)	1745-3682
ISSN	1745-3674
DOI	10.3109/17453674.2016.1165558
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uk I Zs.99: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Cutibacterium acnes clonal complexes display various growth rates in blood culture vials used for diagnosing orthopedic device-related infections.

El Sayed, Faten / Jeverica, Samo / Roux, Anne-Laure / Bauer, Thomas / Nkam, Lionelle / Sivadon-Tardy, Valérie / Noussair, Latifa / Herrmann, Jean-Louis / Gaillard, Jean-Louis / Rak, Mitja / Papst, Lea / Rottman, Martin

Anaerobe

2021 Volume 72, Page(s) 102469

Abstract: Objectives: Blood culture bottles (BCBs) are commonly used for the diagnosis of infections associated with orthopedic devices. Although Cutibacterium acnes is an important pathogen in orthopedics, relatively little is known about its growth ... ...

Abstract	Objectives: Blood culture bottles (BCBs) are commonly used for the diagnosis of infections associated with orthopedic devices. Although Cutibacterium acnes is an important pathogen in orthopedics, relatively little is known about its growth characteristics in BCBs. This prompted us to analyze the influence of bacterial genotype and clinical significance on time-to-detection (TTD) in BCBs. Methods: We reviewed 59 cases of orthopedic device-related infections in which at least one intraoperative specimen yielded a pure C. acnes culture from anaerobic BCBs (BD Bactec Lytic/10 Anaerobic/F; Lytic-Ana) and/or solid media. A strain was considered infectant if the same genotype was present in two or more intraoperative samples. From these cases, we isolated a total of 72 unique C. acnes strains belonging to four multilocus sequence type clonal complexes (CCs): CC18, CC28, CC36 and CC53. Growth rate and TTD in Lytic-Ana BCB were studied under experimental conditions (inoculation of standard inoculum) and in clinical samples (inoculation of periprosthetic tissue samples). Results: Median TTD values were shorter for CC53 compared to other CCs under experimental conditions (69 vs. 103 h; p < 0.001) and from clinical specimens (70 vs. 200 h; p = 0.02). Infectant strains had a shorter median TTD compared to contaminant strains in a clinical situation, while the difference was not observed under experimental conditions. Conclusions: The detection dynamics of C. acnes in Lytic-Ana BCBs were associated with genotype. Thus, TTD not only reflects the bacterial load in clinical samples, but may also reflect the intrinsic properties of the clonal complex of C. acnes.
MeSH term(s)	Adult ; Aged ; Bacterial Proteins/genetics ; Bacterial Typing Techniques ; Blood Culture ; Female ; Gram-Positive Bacterial Infections/diagnosis ; Gram-Positive Bacterial Infections/microbiology ; Humans ; Male ; Middle Aged ; Multilocus Sequence Typing ; Orthopedic Procedures/adverse effects ; Propionibacterium acnes/classification ; Propionibacterium acnes/genetics ; Propionibacterium acnes/isolation & purification ; Prosthesis-Related Infections/diagnosis ; Prosthesis-Related Infections/etiology
Chemical Substances	Bacterial Proteins
Language	English
Publishing date	2021-10-23
Publishing country	England
Document type	Journal Article
ZDB-ID	1237621-8
ISSN	1095-8274 ; 1075-9964
ISSN (online)	1095-8274
ISSN	1075-9964
DOI	10.1016/j.anaerobe.2021.102469
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4318: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Cutibacterium acnes clonal complexes display various growth rates in blood culture vials used for diagnosing orthopedic device-related infections

Anaerobe. 2021 Dec., v. 72

2021

Abstract: Blood culture bottles (BCBs) are commonly used for the diagnosis of infections associated with orthopedic devices. Although Cutibacterium acnes is an important pathogen in orthopedics, relatively little is known about its growth characteristics in BCBs. ... ...

Abstract	Blood culture bottles (BCBs) are commonly used for the diagnosis of infections associated with orthopedic devices. Although Cutibacterium acnes is an important pathogen in orthopedics, relatively little is known about its growth characteristics in BCBs. This prompted us to analyze the influence of bacterial genotype and clinical significance on time-to-detection (TTD) in BCBs.We reviewed 59 cases of orthopedic device-related infections in which at least one intraoperative specimen yielded a pure C. acnes culture from anaerobic BCBs (BD Bactec Lytic/10 Anaerobic/F; Lytic-Ana) and/or solid media. A strain was considered infectant if the same genotype was present in two or more intraoperative samples. From these cases, we isolated a total of 72 unique C. acnes strains belonging to four multilocus sequence type clonal complexes (CCs): CC18, CC28, CC36 and CC53. Growth rate and TTD in Lytic-Ana BCB were studied under experimental conditions (inoculation of standard inoculum) and in clinical samples (inoculation of periprosthetic tissue samples).Median TTD values were shorter for CC53 compared to other CCs under experimental conditions (69 vs. 103 h; p < 0.001) and from clinical specimens (70 vs. 200 h; p = 0.02). Infectant strains had a shorter median TTD compared to contaminant strains in a clinical situation, while the difference was not observed under experimental conditions.The detection dynamics of C. acnes in Lytic-Ana BCBs were associated with genotype. Thus, TTD not only reflects the bacterial load in clinical samples, but may also reflect the intrinsic properties of the clonal complex of C. acnes.
Keywords	blood ; genotype ; inoculum ; microbial load ; multilocus sequence typing ; orthopedics ; pathogens
Language	English
Dates of publication	2021-12
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1237621-8
ISSN	1075-9964
ISSN	1075-9964
DOI	10.1016/j.anaerobe.2021.102469
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 4318: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Identification of the same species in at least two intra-operative samples for prosthetic joint infection diagnostics yields the best results with broad-range polymerase chain reaction.

Rak, Mitja / Barlič-Maganja, Darja / Kavčič, Martina / Trebše, Rihard / Cőr, Andrej

International orthopaedics

2014 Volume 39, Issue 5, Page(s) 975–979

Abstract: Purpose: Prosthetic joint infection (PJI) is a devastating complication of total joint arthroplasty. No single laboratory test has perfect sensitivity and specificity; however, culture of periprosthetic tissue is the standard method for PJI diagnosis. ... ...

Abstract	Purpose: Prosthetic joint infection (PJI) is a devastating complication of total joint arthroplasty. No single laboratory test has perfect sensitivity and specificity; however, culture of periprosthetic tissue is the standard method for PJI diagnosis. Interpretation of positive culture results in PJI diagnostics can be difficult due to the possibility of contamination with microorganisms originating from skin micro flora. Criteria have been established to aid in distinguishing pathogen from contaminant for culture results. A similar criterion has not however been established for polymerase chain reaction (PCR) analysis, which is in part responsible for confusion about the reliability of PCR for PJI diagnostics. The aim of our study was to establish a criterion for interpretation of broad range (BR) PCR results in PJI diagnostics. Methods: Samples of periprosthetic tissue were retrieved from 100 patients with joint prosthesis failure and analysed with BR-PCR. The results of BR-PCR were evaluated based on the number of samples of periprosthetic tissue with the same bacterial species. Results: The sensitivity (87.5%) of BR-PCR was highest if the same species was present in at least one sample, although this criterion also resulted in the lowest specificity (92.1%). The sensitivity decreased (83.2%), although without a statistically significant difference, if the same species was present in two or more samples but, at the same time, specificity increased (100%), with a statistically significant difference. Conclusions: For diagnostics of PJI with BR-PCR the criterion of the same bacterial species in at least two specimens of periprosthetic tissue from the same patient should be used for interpretation of positive results.
MeSH term(s)	Aged ; Aged, 80 and over ; Arthroplasty ; Arthroplasty, Replacement ; Bacterial Infections/microbiology ; Female ; Humans ; Intraoperative Period ; Joint Prosthesis/microbiology ; Male ; Middle Aged ; Polymerase Chain Reaction/methods ; Prospective Studies ; Prosthesis Failure ; Prosthesis-Related Infections/diagnosis ; Prosthesis-Related Infections/microbiology ; Reproducibility of Results ; Sensitivity and Specificity
Language	English
Publishing date	2014-10-19
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80384-4
ISSN	1432-5195 ; 0341-2695
ISSN (online)	1432-5195
ISSN	0341-2695
DOI	10.1007/s00264-014-2552-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1324: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Comparison of molecular and culture method in diagnosis of prosthetic joint infection.

Rak, Mitja / Barlič-Maganja, Darja / Kavčič, Martina / Trebše, Rihard / Cőr, Andrej

FEMS microbiology letters

2013 Volume 343, Issue 1, Page(s) 42–48

Abstract: Diagnosis of prosthetic joint infection with culture technique can be problematic since the causative agent(s) are not possible to cultivate in all cases. Molecular methods had been evaluated in many studies but their inclusion in routine diagnostics is ... ...

Abstract	Diagnosis of prosthetic joint infection with culture technique can be problematic since the causative agent(s) are not possible to cultivate in all cases. Molecular methods had been evaluated in many studies but their inclusion in routine diagnostics is still controversial. The purpose of our prospective study was to compare the diagnostic accuracy of broad-range (BR)-PCR and culture technique. Intraoperative samples of periprosthetic tissue were retrieved in 67 patients undergoing revision arthroplasty. Samples were analyzed with culture technique, immunohistochemistry and BR 16S rRNA gene PCR. Bacteria in PCR-positive samples were identified using two different methods: direct sequencing of PCR products and specific TaqMan assays. In 63 cases, full concordance was found between BR-PCR and culture technique. Specific TaqMan assays failed to identify bacteria in four culture- and BR-PCR-positive cases and therefore had a lower sensitivity in comparison with BR-PCR. Molecular methods detected bacteria with the same accuracy as culture; however, identification of bacteria was inferior to culture. Further development of species-recognition techniques is required to improve identification of causative microorganisms.
MeSH term(s)	Aged ; Aged, 80 and over ; Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification ; Bacterial Infections/diagnosis ; Bacterial Infections/microbiology ; Bacteriological Techniques/methods ; Female ; Humans ; Male ; Middle Aged ; Molecular Diagnostic Techniques/methods ; Osteoarthritis/diagnosis ; Osteoarthritis/microbiology ; Polymerase Chain Reaction/methods ; Prospective Studies ; Prosthesis-Related Infections/diagnosis ; Prosthesis-Related Infections/microbiology ; RNA, Bacterial/genetics ; RNA, Ribosomal, 16S/genetics ; Sensitivity and Specificity
Chemical Substances	RNA, Bacterial ; RNA, Ribosomal, 16S
Language	English
Publishing date	2013-06
Publishing country	England
Document type	Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1111/1574-6968.12125
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1372: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study.

Pascual, Stéphanie / Noble, Brooklyn / Ahmad-Saeed, Nusreen / Aldridge, Catherine / Ambretti, Simone / Amit, Sharon / Annett, Rachel / O'Shea, Shaan Ashk / Barbui, Anna Maria / Barlow, Gavin / Barrett, Lucinda / Berth, Mario / Bondi, Alessandro / Boran, Nicola / Boyd, Sara E / Chaves, Catarina / Clauss, Martin / Davies, Peter / Dianzo-Delgado, Ileana T /

Esteban, Jaime / Fuchs, Stefan / Friis-Hansen, Lennart / Goldenberger, Daniel / Golle, Andrej / Groonroos, Juha O / Hoffmann, Ines / Hoffmann, Tomer / Hughes, Harriet / Ivanova, Marina / Jezek, Peter / Jones, Gwennan / Ceren Karahan, Zeynep / Lass-Flörl, Cornelia / Laurent, Frédéric / Leach, Laura / Horsbøll Pedersen, Matilde Lee / Loiez, Caroline / Lynch, Maureen / Maloney, Robert J / Marsh, Martin / Milburn, Olivia / Mitchell, Shanine / Moore, Luke S P / Moffat, Lynn / Murdjeva, Marianna / Murphy, Michael E / Nayar, Deepa / Nigrisoli, Giacomo / O'Sullivan, Fionnuala / Öz, Büşra / Peach, Teresa / Petridou, Christina / Prinz, Mojgan / Rak, Mitja / Reidy, Niamh / Rossolini, Gian Maria / Roux, Anne-Laure / Ruiz-Garbajosa, Patricia / Saeed, Kordo / Salar-Vidal, Llanos / Salas Venero, Carlos / Selvaratnam, Mathyruban / Senneville, Eric / Starzengruber, Peter / Talbot, Ben / Taylor, Vanessa / Trebše, Rihard / Wearmouth, Deborah / Willinger, Birgit / Wouthuyzen-Bakker, Marjan / Couturier, Brianne / Allantaz, Florence

Journal of bone and joint infection

2024 Volume 9, Issue 1, Page(s) 87–97

Abstract: ... ...

Abstract	Introduction
Language	English
Publishing date	2024-02-28
Publishing country	Germany
Document type	Journal Article
ISSN	2206-3552
ISSN	2206-3552
DOI	10.5194/jbji-9-87-2024
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Book ; Online: Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections

Pascual, Stéphanie / Noble, Brooklyn / Ahmad-Saeed, Nusreen / Aldridge, Catherine / Ambretti, Simone / Amit, Sharon / Annett, Rachel / O'Shea, Shaan Ashk / Barbui, Anna Maria / Barlow, Gavin / Barrett, Lucinda / Berth, Mario / Bondi, Alessandro / Boran, Nicola / Boyd, Sara E. / Chaves, Catarina / Clauss, Martin / Davies, Peter / Dianzo-Delgado, Ileana T. /

Esteban, Jaime / Fuchs, Stefan / Friis-Hansen, Lennart / Goldenberger, Daniel / Golle, Andrej / Groonroos, Juha O. / Hoffmann, Ines / Hoffmann, Tomer / Hughes, Harriet / Ivanova, Marina / Jezek, Peter / Jones, Gwennan / Ceren Karahan, Zeynep / Lass-Flörl, Cornelia / Laurent, Frédéric / Leach, Laura / Horsbøll Pedersen, Matilde Lee / Loiez, Caroline / Lynch, Maureen / Maloney, Robert J. / Marsh, Martin / Milburn, Olivia / Mitchell, Shanine / Moore, Luke S. P. / Moffat, Lynn / Murdjeva, Marianna / Murphy, Michael E. / Nayar, Deepa / Nigrisoli, Giacomo / O'Sullivan, Fionnuala / Öz, Büşra / Peach, Teresa / Petridou, Christina / Prinz, Mojgan / Rak, Mitja / Reidy, Niamh / Rossolini, Gian Maria / Roux, Anne-Laure / Ruiz-Garbajosa, Patricia / Saeed, Kordo / Salar-Vidal, Llanos / Salas Venero, Carlos / Selvaratnam, Mathyruban / Senneville, Eric / Starzengruber, Peter / Talbot, Ben / Taylor, Vanessa / Trebše, Rihard / Wearmouth, Deborah / Willinger, Birgit / Wouthuyzen-Bakker, Marjan / Couturier, Brianne / Allantaz, Florence

eISSN: 2206-3552

a real-world evidence study

2024

Abstract: Introduction : The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint ... ...

Abstract	Introduction : The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods : A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results : A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4 % and 85 % respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus , Streptococcus species, Enterococcus faecalis , Kingella kingae , Neisseria gonorrhoeae , and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1 h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion : The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting.
Subject code	616
Language	English
Publishing date	2024-02-28
Publishing country	de
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED