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Article: Potentials of enhancing the physicochemical and functional characteristics of Nigella sativa oil by using the screw pressing technique for extraction

Hamed, Said / Ramadan Taha, Asmaa / Edris, Amr

Grasas y aceites, 68(2):e188

2017

Abstract: In the current investigation the crude oil of Nigella sativa was extracted from seeds using hydraulic and screw pressing techniques. Different parameters were evaluated in order to find out the appropriate technique to enhance the physicochemical and ... ...

Abstract	In the current investigation the crude oil of Nigella sativa was extracted from seeds using hydraulic and screw pressing techniques. Different parameters were evaluated in order to find out the appropriate technique to enhance the physicochemical and functional-related characteristics of the extracted crude oil. Results showed that the acid and peroxide values were significantly lower in the screw pressed oil (SPO) than in the hydraulic pressed oil (HPO). The total phenolic content of the SPO was significantly higher than that of HPO. Evaluation of the oxidative stability using the Rancimat test showed that SPO recorded a much higher oxidative stability index (40.07 h) than HPO (0.51 h). The yield of the volatile oil fraction and its contents of thymoquinone isolated from the SPO were higher than that from the HPO. Biological evaluation revealed that the SPO had significantly higher antimicrobial activity than HPO against Listeria monocytogenes and Staphylococcus aureus at 40 μL/well. Mejoras potenciales de las características físico-químicas y funcionales del aceite de Nigella sativa mediante extracción con prensa de tornillo. En la presente investigación se extrajo el aceite crudo de Nigella sativa de las semillas utilizando técnicas hidráulicas y de prensado de tornillo. Se evaluaron diferentes parámetros para conocer la técnica apropiada que potencie las características fisicoquímicas y funcionales del aceite extraído. Los resultados mostraron que los valores de ácido y peróxido fueron significativamente más bajos en el aceite de presión con tornillo (SPO) que en el aceite prensado hidráulico (HPO). El contenido fenólico total del SPO fue significativamente mayor que el de HPO. La evaluación de la estabilidad oxidativa mediante Rancimat demostró que el aceite SPO tiene un índice de estabilidad oxidativa mucho más alto (40,07 h) que el HPO (0,51 h). El rendimiento de la fracción volátil y su contenido de timoquinona aislada del aceite SPO fueron superiores a los del HPO. La evaluación biológica reveló que el aceite SPO tenía una actividad antimicrobiana significativamente mayor que el HPO contra Listeria monocitogenes y Staphylococcus aureus a 40 μL/pocillo.
Keywords	Crude oil ; Functionality ; Hydraulic pressing ; Nigella sativa ; Screw pressing
Language	English
Document type	Article
Database	Repository for Life Sciences

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Article: The combined effect of water deficit stress and TiO2 nanoparticles on cell membrane and antioxidant enzymes in Helianthus annuus L

Ramadan, Taha / Sayed, Suzan A. / Abd-Elaal, Amna K. A. / Amro, Ahmed

Physiology and molecular biology of plants. 2022 Feb., v. 28, no. 2

2022

Abstract: Nanotechnology has become one of the several approaches attempting to ameliorate the severe effect of drought on plant's production and to increase the plants tolerance against water deficit for the water economy. In this research, the effect of foliar ... ...

Abstract	Nanotechnology has become one of the several approaches attempting to ameliorate the severe effect of drought on plant's production and to increase the plants tolerance against water deficit for the water economy. In this research, the effect of foliar application of TiO₂, nanoparticles or ordinary TiO₂, on Helianthus annuus subjected to different levels of water deficit was studied. Cell membrane injury increased by increasing the level of water deficit and TiO₂ concentration, and both types of TiO₂ affected the leaves in analogous manner. Ord-TiO₂ increased H₂O₂ generation by 67–240% and lipid peroxidation by 4–67% in leaves. These increases were more than that induced by Nano-TiO₂ and the effect was concentration dependent. Proline significantly increased in leaves by water deficit stress, reaching at 25% field capacity (FC) to more than fivefold compared to that in plants grown on full FC. Spraying plants with water significantly decreased the activities of enzymes in the water deficit stressed roots. The water deficit stress exerted the highest magnitude of effect on the changes of cell membrane injury, MDA, proline content, and activities of CAT and GPX. Nano-TiO₂ was having the highest effect on contents of H₂O₂ and GPX activity. In roots, the level of water deficit causes highest effect on enzyme activities, but TiO₂ influenced more on the changes of MDA and H₂O₂ contents. GPX activity increased by 283% in leaves of plants treated with 50 and 150 ppm Nano-TiO₂, while increased by 170% in those treated with Ord-TiO₂, but APX and CAT activities increased by 17–197%, in average, with Ord-TiO₂. This study concluded that Nano-TiO₂ didn’t ameliorate the effects of drought stress on H. annuus but additively increased the stress, so its use in nano-phytotechnology mustn’t be expanded without extensive studies.
Keywords	Helianthus annuus ; cell membranes ; drought ; field capacity ; foliar application ; lipid peroxidation ; molecular biology ; physiology ; proline ; titanium dioxide ; water stress
Language	English
Dates of publication	2022-02
Size	p. 391-409.
Publishing place	Springer India
Document type	Article
ZDB-ID	2487126-6
ISSN	0974-0430 ; 0971-5894
ISSN (online)	0974-0430
ISSN	0971-5894
DOI	10.1007/s12298-022-01153-z
Database	NAL-Catalogue (AGRICOLA)

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Article: The combined effect of water deficit stress and TiO

Ramadan, Taha / Sayed, Suzan A / Abd-Elaal, Amna K A / Amro, Ahmed

Physiology and molecular biology of plants : an international journal of functional plant biology

2022 Volume 28, Issue 2, Page(s) 391–409

Abstract	Nanotechnology has become one of the several approaches attempting to ameliorate the severe effect of drought on plant's production and to increase the plants tolerance against water deficit for the water economy. In this research, the effect of foliar application of TiO Supplementary information: The online version contains supplementary material available at 10.1007/s12298-022-01153-z.
Language	English
Publishing date	2022-03-15
Publishing country	India
Document type	Journal Article
ZDB-ID	2487126-6
ISSN	0974-0430 ; 0971-5894
ISSN (online)	0974-0430
ISSN	0971-5894
DOI	10.1007/s12298-022-01153-z
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Article ; Online: Re-sternotomy for aortic valve replacement in octogenarian patients in age of evolving transcatheter therapies.

Luthra, Suvitesh / Malvindi, Pietro G / Masraf, Hannah / Podonyi, Anna / Ramadan, Taha / Ohri, Sunil K

Journal of cardiac surgery

2022 Volume 37, Issue 5, Page(s) 1263–1271

Abstract: Background: The aim of this study was to analyze perioperative results and long-term survival of re-sternotomy for surgical aortic valve replacement (SAVR) in octogenarians in age of transcatheter therapies.: Methods: This is a retrospective, single- ... ...

Abstract	Background: The aim of this study was to analyze perioperative results and long-term survival of re-sternotomy for surgical aortic valve replacement (SAVR) in octogenarians in age of transcatheter therapies. Methods: This is a retrospective, single-center study (April 2000 to December 2019). Perioperative data were compared for re-sternotomy with isolated SAVR (Isolated redoSAVR) and re-sternotomy with SAVR and concomitant cardiac procedure (Associated redoSAVR). Regression analyses were performed to identify predictors of in-patient mortality. Hazard ratios and Kaplan-Meier survival curves were compared for groups. Results: There were 163 patients (Isolated redoSAVR; 69, Associated redoSAVR; 94). Emergency/salvage cases were excluded. The median age was 83 (81-85) years and the median logEuroSCORE was 19.2 (13.0-26.7)%. The follow-up was 4.2 ± 3.5 years. Inpatient mortality was 4.9% (1.4% vs. 7.4% for Isolated redoSAVR and Associated redoSAVR respectively, p = .08). TIA/stroke rate was 8% (9% vs. 7% for Isolated redoSAVR and Associated redoSAVR, respectively, p = .78). COPD was a predictor of inpatient mortality (odds ratio: 8.86; 95% confidence interval: 1.19-66.11, p = .03). Survival was 88.7%, 86.4%, 70.1%, 49.5%, and 26.3% at 1, 2, 5, 7, and 10 years. There was no survival difference between Isolated redoSAVR and Associated redoSAVR (log rank p = .36, Wilcoxon p = .84). Significant adverse predictors of long-term survival were COPD, postoperative TIA/stroke, and length of stay. Survival was lower than age and gender-matched first-time SAVR and general population of the United Kingdom. Conclusion: RedoSAVR in octogenarians is associated with significant morbidity and mortality. Shared decision-making should consider emerging transcatheter therapies as a valuable option in selected patients.
MeSH term(s)	Aged, 80 and over ; Aortic Valve/surgery ; Aortic Valve Stenosis ; Heart Valve Prosthesis Implantation/methods ; Humans ; Octogenarians ; Retrospective Studies ; Risk Factors ; Sternotomy ; Transcatheter Aortic Valve Replacement/methods ; Treatment Outcome
Language	English
Publishing date	2022-02-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	639059-6
ISSN	1540-8191 ; 0886-0440
ISSN (online)	1540-8191
ISSN	0886-0440
DOI	10.1111/jocs.16335
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Article: New antimicrobial and cytotoxic benzofuran glucoside from Senecio glaucus L

Zaher, Ahmed M. / Sultan, Raoof / Ramadan, Taha / Amro, Ahmed

Natural product research. 2021 Dec. 22, v. 36, no. 1

2021

Abstract: The ethyl acetate fraction of the dried aerial parts of Senecio glaucus L. exhibited significant antimicrobial activity against some of selected bacteria and fungi. Also, it showed potent cytotoxicity against PANC-1 cancer cell lines under glucose ... ...

Abstract	The ethyl acetate fraction of the dried aerial parts of Senecio glaucus L. exhibited significant antimicrobial activity against some of selected bacteria and fungi. Also, it showed potent cytotoxicity against PANC-1 cancer cell lines under glucose deficient medium. The ethyl acetate fraction was subjected to different chromatographic techniques for isolation of the bioactive compounds. A new benzofuran glucoside; 2,3-dihydro-3β-hydroxyeuparin 3-O-glucopyranoside (1) was isolated. Additionally, two known flavonoid compounds isorhamentin 3-O-β-D-glucoside (2), and isorhamentin 3-O-β-D-rutinoside (3) were first identified in S. glaucus. Compound 1 exhibited potent antimicrobial activities against two Gram-positive bacteria, one Gram-negative bacteria, and two fungi. Also, it displayed potent cytotoxic activity against PANC-1 cancer cell lines under glucose deficient medium (IC50 7.5 μM). However, the isolated flavonoid glycosides (2 & 3) showed moderate antimicrobial activities against two Gram-positive bacteria, two Gram-negative bacteria, four fungi, and did not show any cytotoxic activity against PANC-1.
Keywords	Senecio ; antimicrobial properties ; chromatography ; cytotoxicity ; ethyl acetate ; flavonoids ; glucose ; glucosides ; inhibitory concentration 50 ; neoplasm cells ; research
Language	English
Dates of publication	2021-1222
Size	p. 136-141.
Publishing place	Taylor & Francis
Document type	Article
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2020.1768089
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Article ; Online: Development of Rift Valley fever (RVF) vaccine by genetic joining of the RVF-glycoprotein Gn with the strong adjuvant subunit B of cholera toxin (CTB) and expression in bacterial system

Essam H. Ibrahim / Ramadan Taha / Hamed A. Ghramh / Mona Kilany

Saudi Journal of Biological Sciences, Vol 26, Iss 7, Pp 1676-

2019 Volume 1681

Abstract: One of the mosquito-borne zoonotic diseases is the Rift Valley fever virus (RVFV). Currently, there is no completely licensed vaccine that can be used to vaccinate animals or humans outside endemic areas. The aim of this work was to use the RVFV ... ...

Abstract	One of the mosquito-borne zoonotic diseases is the Rift Valley fever virus (RVFV). Currently, there is no completely licensed vaccine that can be used to vaccinate animals or humans outside endemic areas. The aim of this work was to use the RVFV glycoprotein (Gn) and the subunit B of cholera toxin (CTB) at gene level and build up fused recombinant vaccine. The gene of CTB was joined to the gene Gn to work as an adjuvant in the resulting fusion protein. The designed merged genes (CTB-Gn) was tested for restriction sites, open reading frames, expected fusion protein tertiary structure and antigenicity using computer software. The insert sequence was submitted to the BioProject (GenBank). The insert was subcloned into the pQE-31 expression plasmid. The target recombinant protein (rCTB-Gn) was expressed in M15 bacteria, purified and identified by protein gel electrophoresis. The insert got the accession No: PRJNA386723. Analysis of the designed rCTB-Gn protein revealed that it had the right 3D structure, immunogenic and at the correct molecular weight. The presence of the CTB in the proposed vaccine will augment its immunogenicity. Doses and protection levels of the vaccine need to be manipulated. Keywords: RVFV, Adjuvant, CTB, Subunit vaccine, Recombinant vaccine
Keywords	Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2019-11-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: New antimicrobial and cytotoxic benzofuran glucoside from

Zaher, Ahmed M / Sultan, Raoof / Ramadan, Taha / Amro, Ahmed

Natural product research

2020 Volume 36, Issue 1, Page(s) 136–141

Abstract: The ethyl acetate fraction of the dried aerial parts ... ...

Abstract	The ethyl acetate fraction of the dried aerial parts of
MeSH term(s)	Anti-Infective Agents/pharmacology ; Benzofurans/pharmacology ; Glucosides/pharmacology ; Microbial Sensitivity Tests ; Plant Extracts/pharmacology ; Senecio/chemistry
Chemical Substances	Anti-Infective Agents ; Benzofurans ; Glucosides ; Plant Extracts
Language	English
Publishing date	2020-05-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2020.1768089
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Article ; Online: Early- and mid-term outcomes of reinterventions for aortic bioprosthesis failure.

Giorgio Malvindi, Pietro / Luthra, Suvitesh / Santarpino, Giuseppe / Ramadan, Taha / Hunduma, Gabriel / Olevano, Carlo / Ohri, Sunil K

Asian cardiovascular & thoracic annals

2022 Volume 30, Issue 7, Page(s) 788–796

Abstract: Background: The aim of this study was to evaluate early- and mid-term results of our actual practice embedding redo aortic valve replacement and transcatheter procedures for aortic bioprosthetic failure.: Methods: Data for aortic valve ... ...

Abstract	Background: The aim of this study was to evaluate early- and mid-term results of our actual practice embedding redo aortic valve replacement and transcatheter procedures for aortic bioprosthetic failure. Methods: Data for aortic valve reinterventions (redo surgical aortic valve replacement, isolated redo aortic valve replacement, and valve-in-valve transcatheter aortic valve implantation, transcatheter valve-in-valve procedure) were collected (2010-2019). Logistic regression analysis was performed to identify predictors favouring the choice of transcatheter against redo surgery. Cox analysis was used to study the association of preoperative variables with survival. Survival probabilities were calculated with Kaplan-Meier analysis and compared using a log-rank test. Results: A total of 125 patients were included (redo surgical aortic valve replacement: 84 patients, valve-in-valve transcatheter aortic valve implantation: 41 patients). Median age was 74 [63-80] years, 58% of the patients were male and the median logistic EuroSCORE was 15 [8-26] %. There was no early mortality. Eighteen patients (redo surgical aortic valve replacement: 15, valve-in-valve transcatheter aortic valve implantation: 3) sustained at least one postoperative complication. At pre-discharge transthoracic echocardiogram, valve-in-valve transcatheter aortic valve implantation had significantly higher trans-prosthetic gradients (mean gradient: valve-in-valve transcatheter aortic valve implantation 18 mmHg vs. redo surgical aortic valve replacement 14 mmHg, Conclusions: Redo surgical aortic valve replacement and valve-in-valve transcatheter aortic valve implantation are both safe and effective for aortic bioprosthetic failure. Further valve-in-valve data are needed to determine the haemodynamic performance of transcatheter prostheses and its impact on long-term outcomes.
MeSH term(s)	Aged ; Aortic Valve/diagnostic imaging ; Aortic Valve/surgery ; Aortic Valve Stenosis/diagnostic imaging ; Aortic Valve Stenosis/surgery ; Bioprosthesis ; Female ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; Humans ; Male ; Prosthesis Failure ; Reoperation ; Risk Factors ; Treatment Outcome
Language	English
Publishing date	2022-04-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1400468-9
ISSN	1816-5370 ; 0218-4923
ISSN (online)	1816-5370
ISSN	0218-4923
DOI	10.1177/02184923221094974
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Article ; Online: Agricultural Expert Systems as an Information Resource

Amany Ramadan Taha

Cybrarians Journal, Iss

2007 Volume 12

Abstract: The research discusses the use and usefulness of Agricultural Expert Systems as information source, Those Expert Systems as very important supporting tools for helping people in the decision making process, also this research discusses the role of ... ...

Abstract	The research discusses the use and usefulness of Agricultural Expert Systems as information source, Those Expert Systems as very important supporting tools for helping people in the decision making process, also this research discusses the role of Central laboratory of Agricultural Expert Systems (CLAES) to support and development the Agricultural Expert System as the main supportive unit to Agricultural Expert Systems in Egypt
Keywords	Expert systems ; Agriculture ; Bibliography. Library science. Information resources ; Z
Language	Arabic
Publishing date	2007-03-01T00:00:00Z
Publisher	Cybrarians: The Arabic Portal for Librarianship and Information
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Silver Nanoparticle Production by Ruta graveolens and Testing Its Safety, Bioactivity, Immune Modulation, Anticancer, and Insecticidal Potentials

Hamed A. Ghramh / Essam H. Ibrahim / Mona Kilnay / Zubair Ahmad / Sadeq K. Alhag / Khalid Ali Khan / Ramadan Taha / Fawziah M. Asiri

Bioinorganic Chemistry and Applications, Vol

2020 Volume 2020

Abstract: Ruta graveolens, a plant belonging to the family Rutaceae, is traditionally used as a medicinal plant and a flavoring agent in food. This work aimed to prepare silver nanoparticles (AgNPs) using the ethanol extract from R. graveolens leaves and test ... ...

Abstract	Ruta graveolens, a plant belonging to the family Rutaceae, is traditionally used as a medicinal plant and a flavoring agent in food. This work aimed to prepare silver nanoparticles (AgNPs) using the ethanol extract from R. graveolens leaves and test different biological activities as well as insecticidal potentials in the extract and extract prepared AgNPs. Dried and powdered R. graveolens leaves were subjected to extraction using ethanol, and this extract was used to synthesize AgNPs. AgNP synthesis was monitored by the change in color, UV spectrophotometry, and electron microscopy (scanning). Fourier transform infrared (FT-IR) spectroscopy was used to monitor the functional groups in the extracts. Immunological, physiological, anticancer, antibacterial, and insecticidal potentials of the extract and its prepared AgNPs were tested. Results showed the ability of the leaf extract to synthesize. SEM examination revealed a spherical shape of AgNPs with a size of 40–45 nm. The extract contained many functional groups as indicated by FT-IR. The extract alone inhibited the growth of normal rat splenic cells, while the extract containing AgNPs stimulated its growth. Extract alone stimulated HeLa cell proliferation and inhibited HepG2 growth, while both cell line growth was inhibited by the extract containing AgNPs. Both the extract and extract with AgNPs were safe on RBCs and did not cause any severe elevation in liver enzymes. The extract alone and with AgNPs showed insecticidal activity against Culex pipiens. Our findings suggest that the R. graveolens leaf extract, alone or with AgNPs, is biologically safe on animal cells and has antibacterial, insecticidal, and immunomodulation potentials.
Keywords	Biotechnology ; TP248.13-248.65 ; Inorganic chemistry ; QD146-197
Subject code	630
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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