LIVIVO - Search results -

Search results

Result 1 - 10 of total 13

Search options

Article ; Online: Evaluation of humoral immune response after yellow fever infection: an observational study on patients from the 2017-2018 sylvatic outbreak in Brazil.

Gonçalves, Andreza Parreiras / Almeida, Letícia Trindade / Rezende, Izabela Maurício de / Fradico, Jordana Rodrigues Barbosa / Pereira, Leonardo Soares / Ramalho, Dario Brock / Pascoal Xavier, Marcelo Antônio / Calzavara Silva, Carlos Eduardo / Monath, Thomas P / LaBeaud, Angelle Desiree / Drumond, Betania Paiva / Campi-Azevedo, Ana Carolina / Martins-Filho, Olindo Assis / Teixeira-Carvalho, Andréa / Alves, Pedro Augusto

Microbiology spectrum

2024 Volume 12, Issue 5, Page(s) e0370323

Abstract: Between 2016 and 2018, Brazil experienced major sylvatic yellow fever (YF) outbreaks that caused hundreds of casualties, with Minas Gerais (MG) being the most affected state. These outbreaks provided a unique opportunity to assess the immune response ... ...

Abstract	Between 2016 and 2018, Brazil experienced major sylvatic yellow fever (YF) outbreaks that caused hundreds of casualties, with Minas Gerais (MG) being the most affected state. These outbreaks provided a unique opportunity to assess the immune response triggered by the wild-type (WT) yellow fever virus (YFV) in humans. The plaque reduction neutralization test (PRNT) is currently the standard method to assess the humoral immune response to YFV by measuring neutralizing antibodies (nAbs). The present study aimed to evaluate the humoral immune response of patients from the 2017-2018 sylvatic YF outbreak in MG with different disease outcomes by using PRNTs with a WT YFV strain, isolated from the 2017-2018 outbreak, and a vaccine YFV strain. Samples from naturally infected YF patients were tested, in comparison with healthy vaccinees. Results showed that both groups presented different levels of nAb against the WT and vaccine strains, and the levels of neutralization against the strains varied homotypically and heterotypically. Results based on the geometric mean titers (GMTs) suggest that the humoral immune response after a natural infection of YFV can reach higher levels than that induced by vaccination (GMT of patients against WT YFV compared to GMT of vaccinees,
MeSH term(s)	Yellow Fever/immunology ; Yellow Fever/epidemiology ; Yellow Fever/virology ; Humans ; Brazil/epidemiology ; Yellow fever virus/immunology ; Yellow fever virus/genetics ; Immunity, Humoral ; Antibodies, Neutralizing/blood ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Disease Outbreaks ; Male ; Yellow Fever Vaccine/immunology ; Female ; Adult ; Middle Aged ; Vaccination ; Neutralization Tests ; Young Adult ; Aged ; Adolescent
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; Yellow Fever Vaccine
Language	English
Publishing date	2024-03-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Observational Study
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.03703-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Wild-type Yellow fever virus in cerebrospinal fluid from fatal cases in Brazil, 2018.

de Rezende, Izabela Mauricio / Cenachi, Adriana Regina Campolina / Costa, Thais Alkifeles / Oliveira, Gabriela Fernanda Garcia / Rabelo, Livia / Menezes, Leticia Mattos / Penido, Indiara / Pereira, Leonardo Soares / Arruda, Matheus Soares / Gonç Alves, Andreza Parreiras / Alves, Pedro Augusto / Kroon, Erna Geessien / Calzavara-Silva, Carlos Eduardo / Ramalho, Dario Brock / Martins-Filho, Olindo Assis / Teixeira-Carvalho, Andrea / LaBeaud, A Desiree / Drumond, Betânia Paiva

Frontiers in virology (Lausanne, Switzerland)

2022 Volume 2

Abstract: Yellow fever virus (YFV) is the causative agent of yellow fever (YF), a hemorrhagic and viscerotropic acute disease. Severe YF has been described in approximately 15-25% of YF patients, with 20-50% of severe YF cases being fatal. Here we analyzed ... ...

Abstract	Yellow fever virus (YFV) is the causative agent of yellow fever (YF), a hemorrhagic and viscerotropic acute disease. Severe YF has been described in approximately 15-25% of YF patients, with 20-50% of severe YF cases being fatal. Here we analyzed cerebrospinal fluid (CSF) samples collected during the YF outbreak in Brazil in 2018, aiming to investigate CNS neuroinvasion in fatal YFV cases. YFV RNA was screened by RT-qPCR targeting the 3'UTR region of the YFV genome in CSF. CSF samples were tested for the presence of anti-YFV IgM and neutralizing antibodies, coupled with routine laboratory examinations. Among the 13 patients studied, we detected anti-YFV IgM in CSF from eight patients and YFV RNA in CSF from five patients. YFV RNA genomic load in CSF samples ranged from 1.75×103 to 5.42×103 RNA copies/mL. We genotyped YFV from three CSF samples that grouped with other YFV samples from the 2018 outbreak in Brazil within the South-American I genotype. Even though descriptions of neurologic manifestations due to wild type YFV (WT-YFV) infection are rare, since the last YF outbreak in Brazil in 2017-2018, a few studies have demonstrated WT-YFV RNA in CSF samples from YF fatal cases. Serological tests indicated the presence of IgM and neutralizing antibodies against YFV in CSF samples from two patients. Although the presence of viral RNA, IgM and neutralizing antibodies in CSF samples could indicate neuroinvasiveness, further studies are needed to better elucidate the role of YFV neuroinvasion and possible impacts in disease pathogenesis.
Language	English
Publishing date	2022-09-09
Publishing country	Switzerland
Document type	Journal Article
ISSN	2673-818X
ISSN (online)	2673-818X
DOI	10.3389/fviro.2022.936191
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Meglumine antimoniate intralesional infiltration for localised cutaneous leishmaniasis: a single arm, open label, phase II clinical trial.

Ramalho, Dario Brock / Silva, Rosiana Estéfane da / Senna, Maria Camilo Ribeiro de / Moreira, Hugo Silva Assis / Pedras, Mariana Junqueira / Avelar, Daniel Moreira de / Saraiva, Lara / Rabello, Ana / Cota, Gláucia

Memorias do Instituto Oswaldo Cruz

2018 Volume 113, Issue 9, Page(s) e180200

Abstract: Background: Cutaneous leishmaniasis (CL) is a world-wide health problem which currently lacks effective, affordable and easy to use therapy. Recently, the meglumine antimoniate (MA) intralesional infiltration was included among the acceptable therapies ... ...

Abstract	Background: Cutaneous leishmaniasis (CL) is a world-wide health problem which currently lacks effective, affordable and easy to use therapy. Recently, the meglumine antimoniate (MA) intralesional infiltration was included among the acceptable therapies for New World leishmaniasis. While this approach is attractive, there is currently little evidence to support its use in Americas. Objectives: The aim of this study was to provide information about effectiveness and safety of a standardised MA intralesional infiltration technique for the treatment of CL. Methods: It is a single-arm phase II clinical trial conducted at a Brazilian referral centre. CL cases with parasitological confirmation presenting a maximum of three CL-compatible skin lesions were treated with weekly MA intralesional infiltration by using a validated technique, up to a maximum of eight infiltrations. Results: A total of 53 patients (62 lesions) were included. Overall, patients received a median of seven infiltrations (IQR25-75% 5-8) over a median treatment period of 43 days (IQR25-75% 28-52 days). The definitive cure rate at D180 was 87% (95% CI:77-96%). The majority of adverse events were local, with mild or moderate intensity. Bacterial secondary infection of the lesion site was observed in 13% of the treated patients, beside two intensity-three adverse events (hypersensitivity reactions).
MeSH term(s)	Adolescent ; Adult ; Aged ; Antiprotozoal Agents/administration & dosage ; Antiprotozoal Agents/adverse effects ; Brazil ; Female ; Humans ; Injections, Intralesional ; Leishmaniasis, Cutaneous/drug therapy ; Leishmaniasis, Cutaneous/physiopathology ; Male ; Meglumine/administration & dosage ; Meglumine/adverse effects ; Middle Aged ; Organometallic Compounds/administration & dosage ; Organometallic Compounds/adverse effects ; Treatment Outcome ; Young Adult
Chemical Substances	Antiprotozoal Agents ; Organometallic Compounds ; Meglumine (6HG8UB2MUY) ; meglumine antimoniate (75G4TW236W)
Language	English
Publishing date	2018-06-21
Publishing country	Brazil
Document type	Clinical Trial, Phase II ; Journal Article
ZDB-ID	953293-6
ISSN	1678-8060 ; 0074-0276
ISSN (online)	1678-8060
ISSN	0074-0276
DOI	10.1590/0074-02760180200
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.33: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Mucosal leishmaniasis: the experience of a Brazilian referral center.

Pedras, Mariana Junqueira / Carvalho, Janaína de Pina / Silva, Rosiana Estéfane da / Ramalho, Dario Brock / Senna, Maria Camilo Ribeiro de / Moreira, Hugo Silva Assis / Martinho, Lorena Zaine Matos / Rabello, Ana / Cota, Gláucia

Revista da Sociedade Brasileira de Medicina Tropical

2018 Volume 51, Issue 3, Page(s) 318–323

Abstract: Introduction: Pentavalent antimonials (Sbv) are the most commonly used drugs for the treatment of mucosal leishmaniasis (ML), despite their high toxicity and only moderate efficacy. The aim of this study was to report therapeutic responses with ... ...

Abstract	Introduction: Pentavalent antimonials (Sbv) are the most commonly used drugs for the treatment of mucosal leishmaniasis (ML), despite their high toxicity and only moderate efficacy. The aim of this study was to report therapeutic responses with different available options for ML. Methods: This study was based on a review of clinical records of 35 patients (24 men and 11 women) treated between 2009 and 2015. Results: The median age of patients was 63 years, and the median duration of the disease was 24 months. Seventeen patients received Sbv, while nine patients were treated with liposomal amphotericin B (AmB), and another nine patients were treated with fluconazole. Patients treated with AmB received a total median accumulated dose of 2550mg. The mean duration of azole use was 120 days, and the daily dose ranged from 450 to 900mg. At the three-month follow-up visit, the cure rate was 35%, 67%, and 22% for Sbv, AmB, and azole groups, respectively. At the six-month follow-up visit, the cure rates for Sbv, AmB, and azole groups were 71%, 78%, and 33%, respectively. Conclusions: There is a scarcity of effective ML treatment alternatives, and based on our observations, fluconazole is not a valid treatment option.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Amphotericin B/therapeutic use ; Antimony/therapeutic use ; Antiprotozoal Agents/therapeutic use ; Female ; Fluconazole/therapeutic use ; Humans ; Leishmaniasis, Mucocutaneous/drug therapy ; Male ; Middle Aged ; Severity of Illness Index ; Treatment Outcome
Chemical Substances	Antiprotozoal Agents ; liposomal amphotericin B ; Amphotericin B (7XU7A7DROE) ; Fluconazole (8VZV102JFY) ; Antimony (9IT35J3UV3)
Language	English
Publishing date	2018-05
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	1038126-0
ISSN	1678-9849 ; 0037-8682
ISSN (online)	1678-9849
ISSN	0037-8682
DOI	10.1590/0037-8682-0478-2017
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2726: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Towards a standard protocol for antimony intralesional infiltration technique for cutaneous leishmaniasis treatment.

Silva, Rosiana Estéfane da / Carvalho, Janaína de Pina / Ramalho, Dario Brock / Senna, Maria Camilo Ribeiro De / Moreira, Hugo Silva Assis / Rabello, Ana / Cota, Erika / Cota, Gláucia

Memorias do Instituto Oswaldo Cruz

2017 Volume 113, Issue 2, Page(s) 71–79

Abstract: BACKGROUND Despite its recognised toxicity, antimonial therapy continues to be the first-line drug for cutaneous leishmaniasis (CL) treatment. Intralesional administration of meglumine antimoniate (MA) represents an alternative that could reduce the ... ...

Abstract	BACKGROUND Despite its recognised toxicity, antimonial therapy continues to be the first-line drug for cutaneous leishmaniasis (CL) treatment. Intralesional administration of meglumine antimoniate (MA) represents an alternative that could reduce the systemic absorption of the drug and its side effects. OBJECTIVES This study aims to validate the standard operational procedure (SOP) for the intralesional infiltration of MA for CL therapy as the first step before the assessment of efficacy and safety related to the procedure. METHODS The SOP was created based on 21 trials retrieved from the literature, direct monitoring of the procedure and consultation with experts. This script was submitted to a formal computer-aided inspection to identify readability, clarity, omission, redundancy and unnecessary information (content validation). For criterion and construct validations, the influence of critical condition changes (compliance with the instructions and professional experience) on outcome conformity (saturation status achievement), tolerability (pain referred) and safety (bleeding) were assessed. FINDINGS The median procedure length was 12 minutes and in 72% of them, patients classified the pain as mild. The bleeding was also classified as mild in 96.6% of the procedures. Full compliance with the SOP was observed in 66% of infiltrations. Despite this, in 100% of the inspected procedures, lesion saturation was observed at the end of infiltration, which means that it tolerates some degree of modification in its execution (robustness) without prejudice to the result. CONCLUSIONS The procedure is reproducible and can be used by professionals without previous training with high success and safety rates.
MeSH term(s)	Antiprotozoal Agents/administration & dosage ; Clinical Protocols/standards ; Humans ; Injections, Intralesional/adverse effects ; Injections, Intralesional/methods ; Leishmaniasis, Cutaneous/drug therapy ; Meglumine/administration & dosage ; Meglumine Antimoniate ; Organometallic Compounds/administration & dosage ; Reproducibility of Results
Chemical Substances	Antiprotozoal Agents ; Organometallic Compounds ; Meglumine (6HG8UB2MUY) ; Meglumine Antimoniate (75G4TW236W)
Language	English
Publishing date	2017-12-13
Publishing country	Brazil
Document type	Journal Article ; Validation Study
ZDB-ID	953293-6
ISSN	1678-8060 ; 0074-0276
ISSN (online)	1678-8060
ISSN	0074-0276
DOI	10.1590/0074-027601700125
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.33: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Serum soluble mediators as prognostic biomarkers for morbidity, disease outcome, and late-relapsing hepatitis in yellow fever patients.

Fradico, Jordana Rodrigues Barbosa / Campi-Azevedo, Ana Carolina / Speziali, Elaine / do Valle Antonelli, Lis Ribeiro / Peruhype-Magalhães, Vanessa / de Rezende, Izabela Maurício / Alves, Pedro Augusto / Pascoal-Xavier, Marcelo Antônio / Pereira, Leonardo Soares / Dutra, Maria Rita Teixeira / Ramalho, Dario Brock / Cenachi, Adriana / de Paula, Ludmila / Santos, Tayrine Araujo / do Carmo Said, Rodrigo Fabiano / Calzavara-Silva, Carlos Eduardo / Coelho-Dos-Reis, Jordana Grazziela Alves / de Magalhães, Clara Ramos / Rabelo, Lara Luíza Cerávolo /

Valim, Valéria / Brito-de-Sousa, Joaquim Pedro / da Costa-Rocha, Ismael Artur / de Souza Gomes, Matheus / Amaral, Laurence Rodrigues / de Lima, Sheila Maria Barbosa / Trindade, Gisela Freitas / Santos, Renata Tourinho / da Silva, Juliana Fernandes Amorim / Monath, Thomas / LaBeaud, Angelle Desiree / Drumond, Betânia Paiva / Martins-Filho, Olindo Assis / Teixeira-Carvalho, Andréa

Clinical immunology (Orlando, Fla.)

2023 Volume 251, Page(s) 109321

Abstract: This study described a soluble mediator storm in acute Yellow Fever/YF infection along the kinetics timeline towards convalescent disease. The analyses of the YF Viral RNAnemia, chemokines, cytokines, and growth factors were performed in YF patients at ... ...

Abstract	This study described a soluble mediator storm in acute Yellow Fever/YF infection along the kinetics timeline towards convalescent disease. The analyses of the YF Viral RNAnemia, chemokines, cytokines, and growth factors were performed in YF patients at acute/(D1-15) and convalescent/(D16-315) phases. Patients with acute YF infection displayed a trimodal viremia profile spreading along D3, D6, and D8-14. A massive storm of mediators was observed in acute YF. Higher levels of mediators were observed in YF with higher morbidity scores, patients under intensive care, and those progressing to death than in YF patients who progress to late-relapsing hepatitis/L-Hep. A unimodal peak of biomarkers around D4-6 with a progressive decrease towards D181-315 was observed in non-L-Hep patients, while a bimodal pattern with a second peak around D61-90 was associated with L-Hep. This study provided a comprehensive landscape of evidence that distinct immune responses drive pathogenesis, disease progression, and L-Hep in YF patients.
MeSH term(s)	Humans ; Yellow Fever/pathology ; Prognosis ; Cytokines ; Biomarkers ; Hepatitis ; Yellow Fever Vaccine
Chemical Substances	Cytokines ; Biomarkers ; Yellow Fever Vaccine
Language	English
Publishing date	2023-04-03
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1459903-x
ISSN	1521-7035 ; 1521-6616
ISSN (online)	1521-7035
ISSN	1521-6616
DOI	10.1016/j.clim.2023.109321
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 880: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: The ongoing COVID-19 epidemic in Minas Gerais, Brazil: insights from epidemiological data and SARS-CoV-2 whole genome sequencing.

Xavier, Joilson / Giovanetti, Marta / Adelino, Talita / Fonseca, Vagner / Barbosa da Costa, Alana Vitor / Ribeiro, Adriana Aparecida / Felicio, Katlin Nascimento / Duarte, Clara Guerra / Ferreira Silva, Marcos Vinicius / Salgado, Álvaro / Lima, Mauricio Teixeira / de Jesus, Ronaldo / Fabri, Allison / Soares Zoboli, Cristiane Franco / Souza Santos, Thales Gutemberg / Iani, Felipe / Ciccozzi, Massimo / Bispo de Filippis, Ana Maria / Teixeira de Siqueira, Marilda Agudo Mendonça /

de Abreu, André Luiz / de Azevedo, Vasco / Ramalho, Dario Brock / Campelo de Albuquerque, Carlos F / de Oliveira, Tulio / Holmes, Edward C / Lourenço, José / Junior Alcantara, Luiz Carlos / Assunção Oliveira, Marluce Aparecida

Emerging microbes & infections

2020 Volume 9, Issue 1, Page(s) 1824–1834

Abstract: The recent emergence of a coronavirus (SARS-CoV-2), first identified in the Chinese city of Wuhan in December 2019, has had major public health and economic consequences. Although 61,888 confirmed cases were reported in Brazil by 28 April 2020, little is ...

Abstract	The recent emergence of a coronavirus (SARS-CoV-2), first identified in the Chinese city of Wuhan in December 2019, has had major public health and economic consequences. Although 61,888 confirmed cases were reported in Brazil by 28 April 2020, little is known about the SARS-CoV-2 epidemic in this country. To better understand the recent epidemic in the second most populous state in southeast Brazil - Minas Gerais (MG) - we sequenced 40 complete SARS-CoV-2 genomes from MG cases and examined epidemiological data from three Brazilian states. Both the genome analyses and the geographical distribution of reported cases indicate for multiple independent introductions into MG. Epidemiological estimates of the reproductive number (R) using different data sources and theoretical assumptions suggest the potential for sustained virus transmission despite a reduction in R from the first reported case to the end of April 2020. The estimated date of SARS-CoV-2 introduction into Brazil was consistent with epidemiological data from the first case of a returned traveller from Lombardy, Italy. These findings highlight the nature of the COVID-19 epidemic in MG and reinforce the need for real-time and continued genomic surveillance strategies to better understand and prepare for the epidemic spread of emerging viral pathogens..
MeSH term(s)	Adult ; Aged ; Betacoronavirus/genetics ; Brazil/epidemiology ; COVID-19 ; Coronavirus Infections/epidemiology ; Coronavirus Infections/transmission ; Female ; Genome, Viral ; Geography ; Humans ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/transmission ; SARS-CoV-2 ; Whole Genome Sequencing ; Young Adult
Keywords	covid19
Language	English
Publishing date	2020-07-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2681359-2
ISSN	2222-1751 ; 2222-1751
ISSN (online)	2222-1751
ISSN	2222-1751
DOI	10.1080/22221751.2020.1803146
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Persistence of Yellow fever virus outside the Amazon Basin, causing epidemics in Southeast Brazil, from 2016 to 2018.

Rezende, Izabela Maurício de / Sacchetto, Lívia / Munhoz de Mello, Érica / Alves, Pedro Augusto / Iani, Felipe Campos de Melo / Adelino, Talita Émile Ribeiro / Duarte, Myrian Morato / Cury, Ana Luísa Furtado / Bernardes, André Felipe Leal / Santos, Tayrine Araújo / Pereira, Leonardo Soares / Dutra, Maria Rita Teixeira / Ramalho, Dario Brock / de Thoisy, Benoit / Kroon, Erna Geessien / Trindade, Giliane de Souza / Drumond, Betânia Paiva

PLoS neglected tropical diseases

2018 Volume 12, Issue 6, Page(s) e0006538

Abstract: Background: Yellow fever (YF) is endemic in the Brazilian Amazon Basin, and sporadic outbreaks take place outside the endemic area in Brazil. Since 2016, YF epidemics have been occurring in Southeast Brazil, with more than 1,900 human cases and more ... ...

Abstract	Background: Yellow fever (YF) is endemic in the Brazilian Amazon Basin, and sporadic outbreaks take place outside the endemic area in Brazil. Since 2016, YF epidemics have been occurring in Southeast Brazil, with more than 1,900 human cases and more than 1,600 epizooties of non-human primates (NHPs) reported until April 2018. Previous studies have demonstrated that Yellow fever virus (YFV) causing outbreaks in 2017 formed a monophyletic group. Methodology/principal findings: Aiming to decipher the origin of the YFV responsible for the recent epidemics, we obtained nucleotide sequences of YFV detected in humans (n = 6) and NHPs (n = 10) from Minas Gerais state during 2017-2018. Next, we performed evolutionary analyses and discussed the results in the light of epidemiological records (official numbers of YFV cases at each Brazilian Federative unit, reported by the Brazilian Ministry of Health). Nucleotide sequences of YFV from Southeast Brazil from 2016 to 2018 were highly conserved and formed a monophyletic lineage (BR-YFV_2016/18) within the genotype South America I. Different clusters were observed within lineage BR-YFV_2016/18, one containing the majority of isolates (from humans and NHPs), indicating the sylvatic transmission of YFV. We also detected a cluster characterized by two synapomorphies (amino acid substitutions) that contained YFV only associated with NHP what should be further investigated. The topology of lineage BR-YFV_2016/18 was congruent with epidemiological and temporal patterns of the ongoing epidemic. YFV isolates detected in 2016, in São Paulo state were located in the most basal position of the lineage, followed by the isolates from Minas Gerais and Espírito Santo obtained in 2017 and 2018. The most recent common ancestor of the lineage BR-YFV_2016/18 dated to 2015 (95% credible intervals = 2014-2016), in a period that was coincident with the reemergence of YFV in the Midwest region of Brazil. Conclusions: The results demonstrated a single introduction of YFV in the Southeast region and the silent viral circulation before the onset of the outbreaks in 2016. Evolutionary analyses combined with epidemiological records supported the idea that BR-YFV_2016/18 was probably introduced from the Midwest into the Southeast region, possibly in São Paulo state. The persistence of YFV in the Southeast region, causing epidemics from 2016 to 2018, suggests that this region presents suitable ecological and climatic conditions for YFV maintenance during the epidemic and interepidemic seasons. This fact poses risks for the establishing of YF enzootic cycles and epidemics, outside the Amazon Basin in Brazil. YF surveillance and studies of viral dynamics deserve particular attention, especially in Midwest, Southeast and neighbor regions which are the main areas historically associated with YF outbreaks outside the Amazon Basin. YFV persistence in Southeast Brazil should be carefully considered in the context of public health, especially for public health decision-makers and researchers.
MeSH term(s)	Aedes/virology ; Animals ; Base Sequence ; Brazil/epidemiology ; Disease Outbreaks ; Epidemics ; Genotype ; Humans ; Primates/virology ; RNA, Viral/genetics ; Seasons ; Yellow Fever/epidemiology ; Yellow Fever/virology ; Yellow fever virus/genetics ; Yellow fever virus/isolation & purification
Chemical Substances	RNA, Viral
Language	English
Publishing date	2018-06-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2429704-5
ISSN	1935-2735 ; 1935-2735
ISSN (online)	1935-2735
ISSN	1935-2735
DOI	10.1371/journal.pntd.0006538
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: The ongoing COVID-19 epidemic in Minas Gerais, Brazil: insights from epidemiological data and SARS-CoV-2 whole genome sequencing

Emerg Microbes Infect

Abstract	The recent emergence of a coronavirus (SARS-CoV-2), first identified in the Chinese city of Wuhan in December 2019, has had major public health and economic consequences. Although 61,888 confirmed cases were reported in Brazil by 28 April 2020, little is known about the SARS-CoV-2 epidemic in this country. To better understand the recent epidemic in the second most populous state in southeast Brazil - Minas Gerais (MG) - we sequenced 40 complete SARS-CoV-2 genomes from MG cases and examined epidemiological data from three Brazilian states. Both the genome analyses and the geographical distribution of reported cases indicate for multiple independent introductions into MG. Epidemiological estimates of the reproductive number (R) using different data sources and theoretical assumptions suggest the potential for sustained virus transmission despite a reduction in R from the first reported case to the end of April 2020. The estimated date of SARS-CoV-2 introduction into Brazil was consistent with epidemiological data from the first case of a returned traveller from Lombardy, Italy. These findings highlight the nature of the COVID-19 epidemic in MG and reinforce the need for real-time and continued genomic surveillance strategies to better understand and prepare for the epidemic spread of emerging viral pathogens..
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #684732
Database	COVID19

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: The ongoing COVID-19 epidemic in Minas Gerais, Brazil

Xavier, Joilson / Giovanetti, Marta / Adelino, Talita / Fonseca, Vagner / da Costa, Alana Vitor Barbosa / Ribeiro, Adriana Aparecida / Felicio, Katlin Nascimento / Duarte, Clara Guerra / Silva, Marcos Vinicius Ferreira / Salgado, Álvaro / Lima, Mauricio Teixeira / de Jesus, Ronaldo / Fabri, Allison / Zoboli, Cristiane Franco Soares / Santos, Thales Gutemberg Souza / Iani, Felipe / Ciccozzi, Massimo / de Filippis, Ana Maria Bispo / de Siqueira, Marilda Agudo Mendonça Teixeira /

de Abreu, André Luiz / de Azevedo, Vasco / Ramalho, Dario Brock / de Albuquerque, Carlos F Campelo / de Oliveira, Tulio / Holmes, Edward C / Lourenço, José / Alcantara, Luiz Carlos Junior / Oliveira, Marluce Aparecida Assunção

insights from epidemiological data and SARS-CoV-2 whole genome sequencing

2020

Abstract	The recent emergence of a coronavirus (SARS-CoV-2), first identified in the Chinese city of Wuhan in December 2019, has had major public health and economic consequences. Although 61,888 confirmed cases were reported in Brazil by 28 April 2020, little is known about the SARS-CoV-2 epidemic in this country. To better understand the recent epidemic in the second most populous state in southeast Brazil - Minas Gerais (MG) - we sequenced 40 complete SARS-CoV-2 genomes from MG cases and examined epidemiological data from three Brazilian states. Both the genome analyses and the geographical distribution of reported cases indicate for multiple independent introductions into MG. Epidemiological estimates of the reproductive number (R) using different data sources and theoretical assumptions suggest the potential for sustained virus transmission despite a reduction in R from the first reported case to the end of April 2020. The estimated date of SARS-CoV-2 introduction into Brazil was consistent with epidemiological data from the first case of a returned traveller from Lombardy, Italy. These findings highlight the nature of the COVID-19 epidemic in MG and reinforce the need for real-time and continued genomic surveillance strategies to better understand and prepare for the epidemic spread of emerging viral pathogens.
Keywords	COVID-19 ; Coronavirus ; covid19
Language	English
Publishing date	2020-01-01
Publishing country	au
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED