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  1. Article ; Online: Digital health and the promise of equity in maternity care: A mixed methods multi-country assessment on the use of information and communication technologies in healthcare facilities in Latin America and the Caribbean.

    Capasso, Ariadna / Colomar, Mercedes / Ramírez, Dora / Serruya, Suzanne / de Mucio, Bremen

    PloS one

    2024  Volume 19, Issue 2, Page(s) e0298902

    Abstract: Introduction: Timely access to maternity care is critical to saving lives. Digital health may serve to bridge the care chasm and advance health equity. Conducted in the aftermath of the COVID-19 pandemic, this cross-sectional mixed-methods study ... ...

    Abstract Introduction: Timely access to maternity care is critical to saving lives. Digital health may serve to bridge the care chasm and advance health equity. Conducted in the aftermath of the COVID-19 pandemic, this cross-sectional mixed-methods study assessed the use of information and communication technologies (ICTs) in healthcare facilities in nine Latin American and Caribbean countries to understand the landscape of ICT use in maternity care and the barriers and facilitators to its adoption.
    Materials and methods: Between April 2021 and September 2022, we disseminated an online survey in English and Spanish among, mainly public, healthcare institutions that provided maternity care in Argentina, Bolivia, Colombia, the Dominican Republic, Ecuador, Guyana, Honduras, Paraguay and Peru. We also interviewed 27 administrators and providers in ministries of health and healthcare institutions.
    Results: Most of the 1877 institutions that answered the survey reported using ICTs in maternity care (N = 1536, 82%), ranging from 96% in Peru to 64% in the Dominican Republic. Of institutions that used ICTs, 59% reported using them more than before or for the first time since the pandemic began. ICTs were most commonly used to provide family planning (64%) and breastfeeding (58%) counseling, mainly by phone (82%). At the facility level, availability of equipment and internet coverage, coupled with skilled human resources, were the main factors associated with ICT use. At country level, government-led initiatives to develop digital health platforms, alongside national investments in the digital infrastructure, were the determining factors in the adoption of ICTs in healthcare provision.
    Conclusion: Digital health for maternity care provision relied on commonly available technology and did not necessitate highly sophisticated systems, making it a sustainable and replicable strategy. However, disparities in access to digital health remain and many facilities in rural and remote areas lacked connectivity. Use of ICTs in maternity care depended on countries' long-term commitments to achieving universal health and digital coverage.
    MeSH term(s) Humans ; Female ; Pregnancy ; Latin America ; Digital Health ; Cross-Sectional Studies ; Pandemics ; Maternal Health Services ; Dominican Republic ; Communication
    Language English
    Publishing date 2024-02-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0298902
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Chronoculture, harnessing the circadian clock to improve crop yield and sustainability.

    Steed, Gareth / Ramirez, Dora Cano / Hannah, Matthew A / Webb, Alex A R

    Science (New York, N.Y.)

    2021  Volume 372, Issue 6541

    Abstract: Human health is dependent on a plentiful and nutritious supply of food, primarily derived from crop plants. Rhythmic supply of light as a result of the day and night cycle led to the evolution of circadian clocks that modulate most plant physiology, ... ...

    Abstract Human health is dependent on a plentiful and nutritious supply of food, primarily derived from crop plants. Rhythmic supply of light as a result of the day and night cycle led to the evolution of circadian clocks that modulate most plant physiology, photosynthesis, metabolism, and development. To regulate crop traits and adaptation, breeders have indirectly selected for variation at circadian genes. The pervasive impact of the circadian system on crops suggests that future food production might be improved by modifying circadian rhythms, engineering the timing of transgene expression, and applying agricultural treatments at the most effective time of day. We describe the applied research required to take advantage of circadian biology in agriculture to increase production and reduce inputs.
    MeSH term(s) Arabidopsis/genetics ; Arabidopsis/growth & development ; Arabidopsis/physiology ; Circadian Clocks/genetics ; Circadian Clocks/physiology ; Circadian Rhythm/genetics ; Circadian Rhythm/physiology ; Crops, Agricultural/genetics ; Crops, Agricultural/growth & development ; Food Supply ; Gene Expression Regulation, Plant ; Genetic Loci ; Plant Breeding/methods ; Selection, Genetic
    Language English
    Publishing date 2021-04-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.abc9141
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  3. Article: New connections between circadian rhythms, photosynthesis, and environmental adaptation

    Cano‐Ramirez, Dora L / Antony N. Dodd

    Plant, cell and environment. 2018 Nov., v. 41, no. 11

    2018  

    Abstract: This article comments on: Circadian rhythms are associated with variation in photosystem II function and photoprotective ... ...

    Abstract This article comments on: Circadian rhythms are associated with variation in photosystem II function and photoprotective mechanisms
    Keywords circadian rhythm ; photosystem II ; plant adaptation ; resistance mechanisms ; solar radiation
    Language English
    Dates of publication 2018-11
    Size p. 2515-2517.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 391893-2
    ISSN 1365-3040 ; 0140-7791
    ISSN (online) 1365-3040
    ISSN 0140-7791
    DOI 10.1111/pce.13346
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: New connections between circadian rhythms, photosynthesis, and environmental adaptation.

    Cano-Ramirez, Dora L / Dodd, Antony N

    Plant, cell & environment

    2018  Volume 41, Issue 11, Page(s) 2515–2517

    Abstract: This article comments on: Circadian rhythms are associated with variation in photosystem II function and photoprotective mechanisms. ...

    Abstract This article comments on: Circadian rhythms are associated with variation in photosystem II function and photoprotective mechanisms.
    MeSH term(s) Circadian Rhythm ; Etiolation ; Photosynthesis ; Photosystem II Protein Complex
    Chemical Substances Photosystem II Protein Complex
    Language English
    Publishing date 2018-06-08
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 391893-2
    ISSN 1365-3040 ; 0140-7791
    ISSN (online) 1365-3040
    ISSN 0140-7791
    DOI 10.1111/pce.13346
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  5. Article ; Online: Na+ Sensitivity of the KAT2-Like Channel Is a Common Feature of Cucurbits and Depends on the S5-P-S6 Segment.

    Wang, Li-Min / Zhao, Li-Na / Shah, Iftikhar Hussain / Ramirez, Dora Cano / Boeglin, Martin / Véry, Anne-Aliénor / Sentenac, Hervé / Zhang, Yi-Dong

    Plant & cell physiology

    2021  Volume 63, Issue 2, Page(s) 279–289

    Abstract: Inhibition of Shaker K+ channel activity by external Na+ was previously reported in the melon (Cucumis melo L.) inwardly rectifying K+ channel MIRK and was hypothesized to contribute to salt tolerance. In this study, two inward Shaker K+ channels, CsKAT2 ...

    Abstract Inhibition of Shaker K+ channel activity by external Na+ was previously reported in the melon (Cucumis melo L.) inwardly rectifying K+ channel MIRK and was hypothesized to contribute to salt tolerance. In this study, two inward Shaker K+ channels, CsKAT2 from cucumber (Cucumis sativus) and ClKAT2 from watermelon (Citrullus lanatus), were identified and characterized in Xenopus oocytes. Both channels were inwardly rectifying K+ channels with higher permeability to potassium than other monovalent cations and more active when external pH was acidic. Similarly to MIRK, their activity displayed an inhibition by external Na+, thus suggesting a common feature in Cucurbitaceae (Cucumis spp., Citrullus spp.). CsKAT2 and ClKAT2 are highly expressed in guard cells. After 24 h of plant treatment with 100 mM NaCl, the three KAT2-like genes were significantly downregulated in leaves and guard cells. Reciprocal chimeras were obtained between MIRK and Na+-insensitive AtKAT2 cDNAs. The chimera where the MIRK S5-P-S6 segment was replaced by that from AtKAT2 no longer showed Na+ sensitivity, while the inverse chimera gained Na+ sensitivity. These results provide evidence that the molecular basis of the channel blockage by Na+ is located in the S5-P-S6 region. Comparison of the electrostatic property in the S5-P-S6 region in AtKAT2 and MIRK revealed four key amino acid residues potentially governing Na+ sensitivity.
    MeSH term(s) Biological Transport ; Oocytes/metabolism ; Plant Leaves ; Potassium/metabolism ; Salt Tolerance ; Sodium/metabolism
    Chemical Substances Sodium (9NEZ333N27) ; Potassium (RWP5GA015D)
    Language English
    Publishing date 2021-12-03
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 208907-5
    ISSN 1471-9053 ; 0032-0781
    ISSN (online) 1471-9053
    ISSN 0032-0781
    DOI 10.1093/pcp/pcab170
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  6. Article ; Online: Plasma Membrane Fluidity: An Environment Thermal Detector in Plants.

    Cano-Ramirez, Dora L / Carmona-Salazar, Laura / Morales-Cedillo, Francisco / Ramírez-Salcedo, Jorge / Cahoon, Edgar B / Gavilanes-Ruíz, Marina

    Cells

    2021  Volume 10, Issue 10

    Abstract: The lipid matrix in cell membranes is a dynamic, bidimensional array of amphipathic molecules exhibiting mesomorphism, which contributes to the membrane fluidity changes in response to temperature fluctuation. As sessile organisms, plants must rapidly ... ...

    Abstract The lipid matrix in cell membranes is a dynamic, bidimensional array of amphipathic molecules exhibiting mesomorphism, which contributes to the membrane fluidity changes in response to temperature fluctuation. As sessile organisms, plants must rapidly and accurately respond to environmental thermal variations. However, mechanisms underlying temperature perception in plants are poorly understood. We studied the thermal plasticity of membrane fluidity using three fluorescent probes across a temperature range of -5 to 41 °C in isolated microsomal fraction (MF), vacuolar membrane (VM), and plasma membrane (PM) vesicles from Arabidopsis plants. Results showed that PM were highly fluid and exhibited more phase transitions and hysteresis, while VM and MF lacked such attributes. These findings suggest that PM is an important cell hub with the capacity to rapidly undergo fluidity modifications in response to small changes of temperatures in ranges spanning those experienced in natural habitats. PM fluidity behaves as an ideal temperature detector: it is always present, covers the whole cell, responds quickly and with sensitivity to temperature variations, functions with a cell free-energy cost, and it is physically connected with potential thermal signal transducers to elicit a cell response. It is an optimal alternative for temperature detection selected for the plant kingdom.
    MeSH term(s) Arabidopsis/physiology ; Arabidopsis/ultrastructure ; Cell Membrane/physiology ; Cell Membrane/ultrastructure ; Fluorescent Dyes/metabolism ; Membrane Fluidity/physiology ; Temperature ; Vacuoles/metabolism ; Vacuoles/ultrastructure
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2021-10-17
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10102778
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  7. Article ; Online: Low-temperature and circadian signals are integrated by the sigma factor SIG5.

    Cano-Ramirez, Dora L / Panter, Paige E / Takemura, Tokiaki / de Fraine, Tara Saskia / de Barros Dantas, Luíza Lane / Dekeya, Richard / Barros-Galvão, Thiago / Paajanen, Pirita / Bellandi, Annalisa / Batstone, Tom / Manley, Bethan F / Tanaka, Kan / Imamura, Sousuke / Franklin, Keara A / Knight, Heather / Dodd, Antony N

    Nature plants

    2023  Volume 9, Issue 4, Page(s) 661–672

    Abstract: Chloroplasts are a common feature of plant cells and aspects of their metabolism, including photosynthesis, are influenced by low-temperature conditions. Chloroplasts contain a small circular genome that encodes essential components of the photosynthetic ...

    Abstract Chloroplasts are a common feature of plant cells and aspects of their metabolism, including photosynthesis, are influenced by low-temperature conditions. Chloroplasts contain a small circular genome that encodes essential components of the photosynthetic apparatus and chloroplast transcription/translation machinery. Here, we show that in Arabidopsis, a nuclear-encoded sigma factor that controls chloroplast transcription (SIGMA FACTOR5) contributes to adaptation to low-temperature conditions. This process involves the regulation of SIGMA FACTOR5 expression in response to cold by the bZIP transcription factors ELONGATED HYPOCOTYL5 and ELONGATED HYPOCOTYL5 HOMOLOG. The response of this pathway to cold is gated by the circadian clock, and it enhances photosynthetic efficiency during long-term cold and freezing exposure. We identify a process that integrates low-temperature and circadian signals, and modulates the response of chloroplasts to low-temperature conditions.
    MeSH term(s) Sigma Factor/genetics ; Sigma Factor/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Temperature ; Arabidopsis/metabolism ; Photosynthesis ; Gene Expression Regulation, Plant
    Chemical Substances Sigma Factor ; Arabidopsis Proteins
    Language English
    Publishing date 2023-03-30
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2055-0278
    ISSN (online) 2055-0278
    DOI 10.1038/s41477-023-01377-1
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  8. Article ; Online: Wheat EARLY FLOWERING 3 affects heading date without disrupting circadian oscillations.

    Wittern, Lukas / Steed, Gareth / Taylor, Laura J / Ramirez, Dora Cano / Pingarron-Cardenas, Gabriela / Gardner, Keith / Greenland, Andy / Hannah, Matthew A / Webb, Alex A R

    Plant physiology

    2022  Volume 191, Issue 2, Page(s) 1383–1403

    Abstract: Plant breeders have indirectly selected for variation at circadian-associated loci in many of the world's major crops, when breeding to increase yield and improve crop performance. Using an eight-parent Multiparent Advanced Generation Inter-Cross (MAGIC) ...

    Abstract Plant breeders have indirectly selected for variation at circadian-associated loci in many of the world's major crops, when breeding to increase yield and improve crop performance. Using an eight-parent Multiparent Advanced Generation Inter-Cross (MAGIC) population, we investigated how variation in circadian clock-associated genes contributes to the regulation of heading date in UK and European winter wheat (Triticum aestivum) varieties. We identified homoeologues of EARLY FLOWERING 3 (ELF3) as candidates for the Earliness per se (Eps) D1 and B1 loci under field conditions. We then confirmed a single-nucleotide polymorphism within the coding region of TaELF3-B1 as a candidate polymorphism underlying the Eps-B1 locus. We found that a reported deletion at the Eps-D1 locus encompassing TaELF3-D1 is, instead, an allele that lies within an introgression region containing an inversion relative to the Chinese Spring D genome. Using Triticum turgidum cv. Kronos carrying loss-of-function alleles of TtELF3, we showed that ELF3 regulates heading, with loss of a single ELF3 homoeologue sufficient to alter heading date. These studies demonstrated that ELF3 forms part of the circadian oscillator; however, the loss of all homoeologues was required to affect circadian rhythms. Similarly, loss of functional LUX ARRHYTHMO (LUX) in T. aestivum, an orthologue of a protein partner of Arabidopsis (Arabidopsis thaliana) ELF3, severely disrupted circadian rhythms. ELF3 and LUX transcripts are not co-expressed at dusk, suggesting that the structure of the wheat circadian oscillator might differ from that of Arabidopsis. Our demonstration that alterations to ELF3 homoeologues can affect heading date separately from effects on the circadian oscillator suggests a role for ELF3 in cereal photoperiodic responses that could be selected for without pleiotropic deleterious alterations to circadian rhythms.
    MeSH term(s) Triticum/genetics ; Arabidopsis/genetics ; Plant Breeding ; Circadian Rhythm/genetics ; Circadian Clocks/genetics ; Gene Expression Regulation, Plant
    Language English
    Publishing date 2022-12-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1093/plphys/kiac544
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  9. Article ; Online: Photosynthesis and circadian rhythms regulate the buoyancy of marimo lake balls.

    Cano-Ramirez, Dora L / Saskia de Fraine, Tara / Griffiths, Olivia G / Dodd, Antony N

    Current biology : CB

    2018  Volume 28, Issue 16, Page(s) R869–R870

    Abstract: Marimo are unusual, attractive and endangered spherical aggregations of the filamentous green macroalga Aegagropila linnaei (Figure 1A-E) [1]. Globally rare, marimo populations persist in cold freshwater lakes in Japan, Iceland and Ukraine. Marimo occupy ...

    Abstract Marimo are unusual, attractive and endangered spherical aggregations of the filamentous green macroalga Aegagropila linnaei (Figure 1A-E) [1]. Globally rare, marimo populations persist in cold freshwater lakes in Japan, Iceland and Ukraine. Marimo occupy both the lake bed and rise to the lake surface [2,3]. Here, we show that marimo buoyancy is conferred by bubbles arising from photosynthesis. We find that light-induced acquisition of buoyancy by marimo is circadian-regulated. We identify that there are circadian rhythms of photosynthesis in marimo, which might explain the circadian rhythm of buoyancy in response to light. This identifies a circadian-regulated buoyancy response in an intriguing and little-studied plant.
    MeSH term(s) Biomass ; Chlorophyta/physiology ; Circadian Rhythm/physiology ; Environment ; Lakes ; Movement ; Photosynthesis/physiology ; Seaweed/physiology
    Language English
    Publishing date 2018-08-20
    Publishing country England
    Document type Letter
    ZDB-ID 1071731-6
    ISSN 1879-0445 ; 0960-9822
    ISSN (online) 1879-0445
    ISSN 0960-9822
    DOI 10.1016/j.cub.2018.07.027
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  10. Article: Effects of MPK3 and MPK6 kinases on the chloroplast architecture and function induced by cold acclimation in Arabidopsis

    Saucedo-García, Mariana / González-Córdova, Carla D. / Ponce-Pineda, I. Giordano / Cano-Ramírez, Dora / Romero-Colín, Fernanda M. / Arroyo-Pérez, Erik E. / King-Díaz, Beatriz / Zavafer, Alonso / Gavilanes-Ruíz, Marina

    Photosynthesis research. 2021 Aug., v. 149, no. 1-2

    2021  

    Abstract: Exposure to low, non-freezing temperatures develops freezing tolerance in many plant species. Such process is called cold acclimation. Molecular changes undergone during cold acclimation are orchestrated by signalling networks including MAP kinases. ... ...

    Abstract Exposure to low, non-freezing temperatures develops freezing tolerance in many plant species. Such process is called cold acclimation. Molecular changes undergone during cold acclimation are orchestrated by signalling networks including MAP kinases. Structure and function of chloroplasts are affected by low temperatures. The aim of this work was to study how the MAP kinases MPK3 and MPK6 are involved in the chloroplast performance upon a long period of cold acclimation. We used Arabidopsis thaliana wild type and mpk3 and mpk6 mutants. Adult plants were acclimated during 7 days at 4 °C and then measurements of PSII performance and chloroplast ultrastructure were carried out. Only the mpk6 acclimated plants showed a high freezing sensitivity. No differences in the PSII function were observed in the plants from the three genotypes exposed to non-acclimated or acclimated conditions. The acclimation of wild-type plants produced severe alterations in the ultrastructure of chloroplast and thylakoids, which was more accentuated in the mpk plants. However, only the mpk6 mutant was unable to internalize the damaged chloroplasts into the vacuole. These results indicate that cold acclimation induces alterations in the chloroplast architecture leading to preserve an optimal performance of PSII. MPK3 and MPK6 are necessary to regulate these morphological changes, but besides, MPK6 is needed to the vacuolization of the damaged chloroplasts, suggesting a role in the chloroplast recycling during cold acclimation. The latter could be quite relevant, since it could explain why this mutant is the only one showing an extremely low freezing tolerance.
    Keywords Arabidopsis thaliana ; acclimation ; adults ; cold ; mitogen-activated protein kinase ; mutants ; photosystem II ; research ; thylakoids ; ultrastructure ; vacuoles
    Language English
    Dates of publication 2021-08
    Size p. 201-212.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 1475688-2
    ISSN 1573-5079 ; 0166-8595
    ISSN (online) 1573-5079
    ISSN 0166-8595
    DOI 10.1007/s11120-021-00852-0
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