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Article: Ectopic expression of DnaJ type-I protein homolog of

Gautam, Ranjana / Meena, Rajesh Kumar / Rampuria, Sakshi / Shukla, Pawan / Kirti, P B

2023 Volume 14, Page(s) 1135552

Abstract: Reduced crop productivity results from altered plant physiological processes caused by dysfunctional proteins due to environmental stressors. In this study, a novel DnaJ Type-I encoding gene, ...

Abstract	Reduced crop productivity results from altered plant physiological processes caused by dysfunctional proteins due to environmental stressors. In this study, a novel DnaJ Type-I encoding gene,
Language	English
Publishing date	2023-04-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2023.1135552
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Global SUMOylome Adjustments in Basal Defenses of

Kasera, Mritunjay / Ingole, Kishor D / Rampuria, Sakshi / Walia, Yashika / Gassmann, Walter / Bhattacharjee, Saikat

Frontiers in cell and developmental biology

2021 Volume 9, Page(s) 680760

Abstract: Steady-state SUMOylome of a plant is adjusted locally during developmental transitions and more globally during stress exposures. We recently reported that basal immunity ... ...

Abstract	Steady-state SUMOylome of a plant is adjusted locally during developmental transitions and more globally during stress exposures. We recently reported that basal immunity in
Language	English
Publishing date	2021-09-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2021.680760
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: An in vitro generated variant of Tephrosia villosa defensin (α-TvD1) enhances biotic stress tolerance in transgenic tobacco

Sharma, Akanksha / Sambasivam, Vijayan / Shukla, Pawan / Rampuria, Sakshi / Bharadwaja, Pulgurtha

Journal of plant pathology. 2020 Nov., v. 102, no. 4

2020

Abstract: Plant defensins are small, cationic, cysteine-rich peptides with well conserved four disulphide bridges between an α-helix and three anti-parallel β-strands, produced by plants as a first line of innate immunity against the invading fungal pathogens. ... ...

Abstract	Plant defensins are small, cationic, cysteine-rich peptides with well conserved four disulphide bridges between an α-helix and three anti-parallel β-strands, produced by plants as a first line of innate immunity against the invading fungal pathogens. Some of the plant defensins exhibit strong antibacterial and anti-insect activities in vitro as well as in vivo. TvD1, a defensin from Tephrosia villosa was characterized in our previous studies with demonstrated inhibitory effect against many plant pathogenic fungi and some insects. To further enhance its activity, α-TvD1, a peptide variant of TvD1, was generated through site directed mutagenesis. It showed enhanced antifungal and Tenebrio molitor α-amylase inhibitory activity under in vitro condition. In the present study, we compared the antifungal and anti-insect activities of α-TvD1 with the native TvD1 in transgenic tobacco plants. α-TvD1 transgenic plants showed enhanced tolerance against the oomycetes pathogen Phytophthora parasitica var. nicotianae and the fungal pathogens Alternaria alternata and Rhizoctonia solani. Also, it showed enhanced anti-insect activity against the 1st and 2nd instar larvae of the generalist herbivore, Spodoptera litura. These results demonstrated that α-TvD1 appears to be a potent antimicrobial gene that can be used for generating superior transgenic crops with enhanced resistance against many phytopathogens and insect pests.
Keywords	Alternaria alternata ; Oomycetes ; Spodoptera litura ; Tenebrio molitor ; Tephrosia ; Thanatephorus cucumeris ; alpha-amylase ; biotic stress ; disulfide bonds ; genes ; herbivores ; innate immunity ; insect pests ; insecticidal properties ; instars ; larvae ; peptides ; plant pathogenic fungi ; plant pathology ; site-directed mutagenesis ; stress tolerance ; tobacco ; transgenic plants
Language	English
Size	p. 1133-1143.
Publishing place	Edizioni ETS
Document type	Article
Note	NAL-light
ZDB-ID	1378427-4
ISSN	2239-7264 ; 1125-4653
ISSN (online)	2239-7264
ISSN	1125-4653
Database	NAL-Catalogue (AGRICOLA)

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Article: Pathogen-induced AdDjSKI of the wild peanut, Arachis diogoi, potentiates tolerance of multiple stresses in E. coli and tobacco

Rampuria, Sakshi / Akanksha Sharma / Conner J. Rogan / P.B. Kirti / Pushan Bag / Walter Gassmann

Plant science. 2018 July, v. 272

2018

Abstract: A gene encoding a serine-rich DnaJIII protein called AdDjSKI that has a 4Fe-4S cluster domain was found to be differentially upregulated in the wild peanut, Arachis diogoi in its resistance responses against the late leaf spot causing fungal pathogen ... ...

Abstract	A gene encoding a serine-rich DnaJIII protein called AdDjSKI that has a 4Fe-4S cluster domain was found to be differentially upregulated in the wild peanut, Arachis diogoi in its resistance responses against the late leaf spot causing fungal pathogen Phaeoisariopsis personata when compared with the cultivated peanut, Arachis hypogaea. AdDjSKI is induced in multiple stress conditions in A. diogoi. Recombinant E. coli cells expressing AdDjSKI showed better growth kinetics when compared with vector control cells under salinity, osmotic, acidic and alkaline stress conditions. Overexpression of this type three J-protein potentiates not only abiotic stress tolerance in Nicotiana tabacum var. Samsun, but also enhances its disease resistance against the phytopathogenic fungi Phytophthora parasitica pv nicotianae and Sclerotinia sclerotiorum. In the present study we show transcriptional upregulation of APX, Mn-SOD and HSP70 under heat stress and increased transcripts of PR genes in response to fungal infection. This transmembrane-domain-containing J protein displays punctate localization in chloroplasts. AdDjSKI appears to ensure proper folding of proteins associated with the photosynthetic machinery under stress.
Keywords	Arachis hypogaea ; chloroplasts ; disease resistance ; Escherichia coli ; gene expression regulation ; genes ; growth models ; heat stress ; leaf spot ; Nicotiana tabacum ; peanuts ; Phaeoisariopsis ; photosynthesis ; Phytophthora nicotianae var. parasitica ; plant pathogenic fungi ; proteins ; salinity ; Sclerotinia sclerotiorum ; stress tolerance ; tobacco ; transcription (genetics) ; vector control
Language	English
Dates of publication	2018-07
Size	p. 62-74.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	742010-9
ISSN	1873-2259 ; 0168-9452
ISSN (online)	1873-2259
ISSN	0168-9452
DOI	10.1016/j.plantsci.2018.03.033
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Pathogen-induced AdDjSKI of the wild peanut, Arachis diogoi, potentiates tolerance of multiple stresses in E. coli and tobacco.

Rampuria, Sakshi / Bag, Pushan / Rogan, Conner J / Sharma, Akanksha / Gassmann, Walter / Kirti, P B

Plant science : an international journal of experimental plant biology

2018 Volume 272, Page(s) 62–74

Abstract	A gene encoding a serine-rich DnaJIII protein called AdDjSKI that has a 4Fe-4S cluster domain was found to be differentially upregulated in the wild peanut, Arachis diogoi in its resistance responses against the late leaf spot causing fungal pathogen Phaeoisariopsis personata when compared with the cultivated peanut, Arachis hypogaea. AdDjSKI is induced in multiple stress conditions in A. diogoi. Recombinant E. coli cells expressing AdDjSKI showed better growth kinetics when compared with vector control cells under salinity, osmotic, acidic and alkaline stress conditions. Overexpression of this type three J-protein potentiates not only abiotic stress tolerance in Nicotiana tabacum var. Samsun, but also enhances its disease resistance against the phytopathogenic fungi Phytophthora parasitica pv nicotianae and Sclerotinia sclerotiorum. In the present study we show transcriptional upregulation of APX, Mn-SOD and HSP70 under heat stress and increased transcripts of PR genes in response to fungal infection. This transmembrane-domain-containing J protein displays punctate localization in chloroplasts. AdDjSKI appears to ensure proper folding of proteins associated with the photosynthetic machinery under stress.
MeSH term(s)	Arachis/genetics ; Arachis/physiology ; Disease Resistance/genetics ; Disease Resistance/physiology ; Escherichia coli/genetics ; Escherichia coli/physiology ; Organisms, Genetically Modified ; Osmotic Pressure/physiology ; Plant Proteins/genetics ; Plant Proteins/physiology ; Plants, Genetically Modified ; RNA, Plant/genetics ; Real-Time Polymerase Chain Reaction ; Salt Tolerance/genetics ; Salt Tolerance/physiology ; Stress, Physiological/genetics ; Stress, Physiological/physiology ; Nicotiana/genetics ; Nicotiana/physiology
Chemical Substances	Plant Proteins ; RNA, Plant
Language	English
Publishing date	2018-04-03
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	742010-9
ISSN	1873-2259 ; 0168-9452
ISSN (online)	1873-2259
ISSN	0168-9452
DOI	10.1016/j.plantsci.2018.03.033
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Article: A novel zinc-binding alcohol dehydrogenase 2 from Arachis diogoi, expressed in resistance responses against late leaf spot pathogen, induces cell death when transexpressed in tobacco.

Kumar, Dilip / Rampuria, Sakshi / Singh, Naveen Kumar / Kirti, Pulugurtha B

FEBS open bio

2016 Volume 6, Issue 3, Page(s) 200–210

Abstract: A novel zinc-binding alcohol dehydrogenase 2 (AdZADH2) was significantly upregulated in a wild peanut, Arachis diogoi treated with conidia of late leaf spot (LLS) pathogen, Phaeoisariopsis personata. This upregulation was not observed in a comparative ... ...

Abstract	A novel zinc-binding alcohol dehydrogenase 2 (AdZADH2) was significantly upregulated in a wild peanut, Arachis diogoi treated with conidia of late leaf spot (LLS) pathogen, Phaeoisariopsis personata. This upregulation was not observed in a comparative analysis of cultivated peanut, which is highly susceptible to LLS. This zinc-binding alcohol dehydrogenase possessed a Rossmann fold containing NADB domain in addition to the MDR domain present in all previously characterized plant ADH genes/proteins. Transient over-expression of AdZADH2 under an estradiol inducible promoter (XVE) resulted in hypersensitive response (HR)-like cell death in tobacco leaf. However, the same level of cell death was not observed when the domains were transiently expressed individually. Cell death observed in tobacco was associated with overexpression of cell death related proteins, antioxidative enzymes such as SOD, CAT and APX and pathogenesis-related (PR) proteins. In A. diogoi, AdZADH2 expression was significantly upregulated in response to the plant signaling hormones salicylic acid, methyl jasmonate, and sodium nitroprusside.
Language	English
Publishing date	2016-03
Publishing country	England
Document type	Journal Article
ISSN	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.12040
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, Arachis diogoi Confers Abiotic Stress Tolerance in Tobacco.

Sharma, Akanksha / Kumar, Dilip / Kumar, Sumit / Rampuria, Sakshi / Reddy, Attipalli R / Kirti, Pulugurtha Bharadwaja

PloS one

2016 Volume 11, Issue 3, Page(s) e0150609

Abstract: Late embryogenesis abundant (LEA) proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants ... ...

Abstract	Late embryogenesis abundant (LEA) proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants against these stresses. A transcript derived fragment (TDF) corresponding to LEA gene, which got differentially expressed in wild peanut, Arachis diogoi against the late leaf spot pathogen, Phaeoisariopsis personata was used in this study. We have cloned its full length cDNA by RACE-PCR, which was designated as AdLEA. AdLEA belongs to the atypical Group 5C of LEA protein family as confirmed by sequence analysis. Group 5C LEA protein subfamily contains Pfam LEA_2 domain and is highly hydrophobic. In native conditions, expression of AdLEA was upregulated considerably upon hormonal and abiotic stress treatments emphasizing its role in abiotic stress tolerance. Subcellular localization studies showed that AdLEA protein is distributed in both nucleus and cytosol. Ectopic expression of AdLEA in tobacco resulted in enhanced tolerance of plants to dehydration, salinity and oxidative stress with the transgenic plants showing higher chlorophyll content and reduced lipid peroxidation as compared to wild type plants. Overexpressed AdLEA tobacco plants maintained better photosynthetic efficiency under drought conditions as demonstrated by chlorophyll fluorescence measurements. These plants showed enhanced transcript accumulation of some stress-responsive genes. Our study also elucidates that ROS levels were significantly reduced in leaves and stomatal guard cells of transgenic plants upon stress treatments. These results suggest that AdLEA confers multiple stress tolerance to plants, which make it a potential gene for genetic modification in plants.
MeSH term(s)	Arachis/genetics ; Arachis/metabolism ; Chlorophyll/genetics ; Chlorophyll/metabolism ; Gene Expression Regulation, Plant ; Plant Leaves/genetics ; Plant Leaves/metabolism ; Plant Leaves/parasitology ; Plant Proteins/biosynthesis ; Plant Proteins/genetics ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism ; Seedlings/genetics ; Seedlings/metabolism ; Seedlings/parasitology ; Stress, Physiological/genetics ; Nicotiana/genetics ; Nicotiana/metabolism ; Nicotiana/parasitology
Chemical Substances	Plant Proteins ; late embryogenesis abundant protein, plant ; Chlorophyll (1406-65-1)
Language	English
Publishing date	2016-03-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0150609
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Characterization of a vacuolar processing enzyme expressed in Arachis diogoi in resistance responses against late leaf spot pathogen, Phaeoisariopsis personata

Kumar, Dilip / Rampuria, Sakshi / Singh, Naveen Kumar / Shukla, Pawan / Kirti, P. B

Plant molecular biology. 2015 May, v. 88, no. 1-2

2015

Abstract: Vacuolar processing enzymes are cysteine proteases responsible for maturation of vacuolar proteins. They have been shown to possess caspase-1-like activity, mediate cell death and display increased activity during pathogen infections. A transcript ... ...

Abstract	Vacuolar processing enzymes are cysteine proteases responsible for maturation of vacuolar proteins. They have been shown to possess caspase-1-like activity, mediate cell death and display increased activity during pathogen infections. A transcript derived fragment corresponding to VPE was found to be up-regulated in a cDNA-AFLP analysis of host responses of a wild peanut, Arachis diogoi upon challenge from the late leaf spot pathogen Phaeoisariopsis personata, which was subsequently validated by q-PCR in a time course analysis, where susceptible peanut did not show its upregulation. In transient conditional and constitutive expression studies in tobacco leaves using agroinfiltration, we have observed that expression of AdVPE was associated with hypersensitive response (HR) like cell death. AdVPE expression was found to be high at 24 h post estradiol application and this was associated with the enhanced co-expression of molecular markers of HR cell death genes and genes for pathogenesis related proteins indicating that AdVPE positively regulates defense responses and its estradiol induced expression is sufficient for HR-like cell death in tobacco. We found that AdVPE expression was very strongly induced in response to sodium nitroprusside, which indicates its involvement in stress signaling. Induced expression of AdVPE in response to jasmonic acid and ethylene also indicates its involvement in an interconnected network of signaling. Transgenic tobacco plants ectopically expressing AdVPE exhibited enhanced resistance against Phytophthora parasitica var. nicotianae, Alternaria alternata var. nicotianae and Rhizoctonia solani. To our knowledge, this is the first report on the heterologous expression of a pathogen induced VPE enhancing resistance to fungal pathogens with cell death phenomenon under transient expression.
Keywords	Alternaria alternata ; Arachis ; Nicotiana tabacum ; Phaeoisariopsis ; Phytophthora nicotianae var. parasitica ; Thanatephorus cucumeris ; agroinfiltration ; cell death ; cysteine proteinases ; disease resistance ; estradiol ; ethylene ; fungi ; gene expression ; genes ; genetic markers ; hypersensitive response ; jasmonic acid ; leaf spot ; leaves ; messenger RNA ; nitroprusside ; pathogenesis ; pathogens ; peanuts ; proteins ; tobacco ; transgenic plants
Language	English
Dates of publication	2015-05
Size	p. 177-191.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	778032-1
ISSN	1573-5028 ; 0167-4412
ISSN (online)	1573-5028
ISSN	0167-4412
DOI	10.1007/s11103-015-0318-x
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Characterization of a vacuolar processing enzyme expressed in Arachis diogoi in resistance responses against late leaf spot pathogen, Phaeoisariopsis personata.

Kumar, Dilip / Rampuria, Sakshi / Singh, Naveen Kumar / Shukla, Pawan / Kirti, P B

Plant molecular biology

2015 Volume 88, Issue 1-2, Page(s) 177–191

Abstract	Vacuolar processing enzymes are cysteine proteases responsible for maturation of vacuolar proteins. They have been shown to possess caspase-1-like activity, mediate cell death and display increased activity during pathogen infections. A transcript derived fragment corresponding to VPE was found to be up-regulated in a cDNA-AFLP analysis of host responses of a wild peanut, Arachis diogoi upon challenge from the late leaf spot pathogen Phaeoisariopsis personata, which was subsequently validated by q-PCR in a time course analysis, where susceptible peanut did not show its upregulation. In transient conditional and constitutive expression studies in tobacco leaves using agroinfiltration, we have observed that expression of AdVPE was associated with hypersensitive response (HR) like cell death. AdVPE expression was found to be high at 24 h post estradiol application and this was associated with the enhanced co-expression of molecular markers of HR cell death genes and genes for pathogenesis related proteins indicating that AdVPE positively regulates defense responses and its estradiol induced expression is sufficient for HR-like cell death in tobacco. We found that AdVPE expression was very strongly induced in response to sodium nitroprusside, which indicates its involvement in stress signaling. Induced expression of AdVPE in response to jasmonic acid and ethylene also indicates its involvement in an interconnected network of signaling. Transgenic tobacco plants ectopically expressing AdVPE exhibited enhanced resistance against Phytophthora parasitica var. nicotianae, Alternaria alternata var. nicotianae and Rhizoctonia solani. To our knowledge, this is the first report on the heterologous expression of a pathogen induced VPE enhancing resistance to fungal pathogens with cell death phenomenon under transient expression.
MeSH term(s)	Amplified Fragment Length Polymorphism Analysis ; Arachis/enzymology ; Arachis/immunology ; Arachis/microbiology ; Ascomycota/physiology ; Cysteine Endopeptidases/genetics ; Cysteine Endopeptidases/metabolism ; DNA, Complementary/genetics ; DNA, Complementary/isolation & purification ; Disease Resistance/immunology ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Phylogeny ; Plant Diseases/immunology ; Plant Diseases/microbiology ; Plant Leaves/immunology ; Plant Leaves/microbiology ; Plants, Genetically Modified ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sequence Alignment ; Stress, Physiological/genetics ; Nicotiana/genetics
Chemical Substances	DNA, Complementary ; RNA, Messenger ; Cysteine Endopeptidases (EC 3.4.22.-) ; vacuolar processing enzyme (EC 3.4.22.-)
Language	English
Publishing date	2015-04-17
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	778032-1
ISSN	1573-5028 ; 0167-4412
ISSN (online)	1573-5028
ISSN	0167-4412
DOI	10.1007/s11103-015-0318-x
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Article: Identification of Heat-Related ESTs in Moth Bean Through Suppression Subtraction Hybridization

Gurjar, Karun / Rampuria, Sakshi / Joshi, Uma / Palit, Paramita / Bhatt, K. V / Mohapatra, T / Sharma, Ramavtar

Applied biochemistry and biotechnology. 2014 Aug., v. 173, no. 8

2014

Abstract: Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward ... ...

Abstract	Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant genotype RMO-40 to identify genes expressing under delayed response to elevated temperature. Heat induction was carried out by exposing 14-day-old seedlings to elevated temperature of 42� °C for 30� min. A total of 125 unigenes (33 contigs and 92 singletons) were derived by cluster assembly and sequence alignment of 200 ESTs; out of 125 unigenes, 21 (16� %) were found to be novel to moth bean. Gene ontology functional classification terms were retrieved for 98 (78.4� %) unigenes of which 73 (58.4� %) ESTs were functionally annotated (GO consensus) where 19 unigenes were annotated with 11 enzyme commission (EC) codes and were mapped to 25 different KEGG pathways. We have identified a majority of heat-shock proteins (constituting 35� % of the present library) aiding heat stress tolerance to moth bean. An expression level of 22 ESTs generated from the above SSH cDNA library was studied through semiquantitative RT-PCR assay simultaneously under 5 and 30� min of heat stress at 42� °C.
Keywords	Vigna aconitifolia ; cDNA libraries ; complementary DNA ; expressed sequence tags ; genotype ; heat ; heat shock proteins ; heat stress ; heat tolerance ; reverse transcriptase polymerase chain reaction ; seedlings ; sequence alignment ; stress tolerance ; suppression subtractive hybridization ; temperature ; unigenes
Language	English
Dates of publication	2014-08
Size	p. 2116-2128.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	392344-7
ISSN	0273-2289
ISSN	0273-2289
DOI	10.1007/s12010-014-1011-2
Database	NAL-Catalogue (AGRICOLA)

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