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Article ; Online: Preoperative diagnosis of intraductal papillary neoplasm of the bile duct with endoscopic ultrasound.

Ramzi, J / Feretis, M / Hickman, K E / Martin, J L / Godfrey, E / Liau, S S

Annals of the Royal College of Surgeons of England

2021 Volume 103, Issue 2, Page(s) e65–e68

Abstract: Intraductal papillary mucinous neoplasm of the bile duct is a rare tumour only recently classified as a distinct pathological entity. These neoplasms, rarely encountered in clinical practice in the UK, are now considered to be important precursors for ... ...

Abstract	Intraductal papillary mucinous neoplasm of the bile duct is a rare tumour only recently classified as a distinct pathological entity. These neoplasms, rarely encountered in clinical practice in the UK, are now considered to be important precursors for the development of cholangiocarcinoma. We present a histologically confirmed case of intraductal papillary neoplasm of the bile duct in a male patient and discuss the main radiographic manifestations of this rare condition across multiple imaging modalities, with an emphasis on the imaging features of endoscopic ultrasonography and its role in establishing the diagnosis.
MeSH term(s)	Adenocarcinoma, Mucinous/diagnosis ; Adenocarcinoma, Mucinous/pathology ; Adenocarcinoma, Mucinous/surgery ; Adenocarcinoma, Papillary/diagnosis ; Adenocarcinoma, Papillary/pathology ; Adenocarcinoma, Papillary/surgery ; Aged ; Anatomic Variation ; Bile Duct Neoplasms/diagnosis ; Bile Duct Neoplasms/pathology ; Bile Duct Neoplasms/surgery ; Bile Ducts, Intrahepatic/abnormalities ; Bile Ducts, Intrahepatic/diagnostic imaging ; Bile Ducts, Intrahepatic/pathology ; Bile Ducts, Intrahepatic/surgery ; Cholangiopancreatography, Endoscopic Retrograde ; Endoscopic Ultrasound-Guided Fine Needle Aspiration ; Endosonography ; Hepatectomy/methods ; Humans ; Incidental Findings ; Male ; Preoperative Care/methods ; Tomography, X-Ray Computed ; Treatment Outcome
Language	English
Publishing date	2021-02-03
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	80044-2
ISSN	1478-7083 ; 0035-8843
ISSN (online)	1478-7083
ISSN	0035-8843
DOI	10.1308/rcsann.2020.7010
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Article ; Online: The influence of palm oil fuel ash heat treatment on the strength activity, porosity, and water absorption of cement mortar

Shaladi, Ramzi J. / Johari, Megat Azmi Megat / Zainal Arifin Ahmad / Mijarsh, Mustafa Juma A.

Environ Sci Pollut Res. 2022 Oct., v. 29, no. 48 p.72493-72514

2022

Abstract: The current study aims to explore the impact of palm oil fuel ash (POFA) heat treatment on the strength activity, porosity, and water absorption of cement mortar. The cement mortar mixtures were typically comprising cement or cement in combination with ... ...

Abstract	The current study aims to explore the impact of palm oil fuel ash (POFA) heat treatment on the strength activity, porosity, and water absorption of cement mortar. The cement mortar mixtures were typically comprising cement or cement in combination with ultrafine treated POFA (u-TPOFA) which is the final form of the treated POFA, sand, water, and a superplasticizer. Before utilizing the u-TPOFA in mortar mixtures, the treatment processes of POFA were undertaken via five steps (drying at 105 ℃, sieving, grinding, heat treatment, re-grinding) to form u-TPOFA. The heat treatment was performed at three different heating temperatures (i.e., 550 ℃, 600 ℃, and 650 ℃). The ratio on mass/mass basis of the blended ordinary Portland cement (OPC) with u-TPOFA was OPC:u-TPOFA of 70%:30%. A total of four mixtures were prepared, consisting of a plain control mixture (designated as PCM) and three mixtures containing 30% of u-TPOFA treated at three different temperatures designated as M1 “550 ℃,” M2 “600 ℃,” and M3 “650 ℃”. The results show that the optimum mixture was M2 which achieved the highest strength activity index (SAI) of 101.84% and 107% among all mixtures at 7 days and 28 days, respectively. Meanwhile, the porosity (P%) and water absorption (Abs%) of M2 exhibited the lowest values of 9.3% and 4.5%, respectively, among all the mixtures at 28 days. This superior performance of u-TPOFA treated at 600 ℃ represented in the M2 mixture was due to the formation of more binding phases consisting of calcium silicate hydrate (C–S–H) type gel originated from a higher pozzolanic reaction and the filler effects caused by the fine u-TPOFA microparticles. These observations were further confirmed by the improved performance of the M2 mix among all the designed mixes which also exhibited better results in terms of bulk density (BD), ultrasonic pulse velocity (UPV), X-ray diffraction (XRD) as well as thermogravimetry (TGA) and field emission scanning electron microscopy (FESEM-EDX) analyses.
Keywords	X-ray diffraction ; bulk density ; calcium silicate ; cement ; electron microscopy ; fuels ; gels ; heat treatment ; palm oils ; porosity ; sand ; thermogravimetry ; ultrasonics ; water uptake
Language	English
Dates of publication	2022-10
Size	p. 72493-72514.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-20710-3
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Article ; Online: The influence of palm oil fuel ash heat treatment on the strength activity, porosity, and water absorption of cement mortar.

Shaladi, Ramzi J / Johari, Megat Azmi Megat / Ahmad, Zainal Arifin / Mijarsh, Mustafa Juma A

Environmental science and pollution research international

2022 Volume 29, Issue 48, Page(s) 72493–72514

Abstract	The current study aims to explore the impact of palm oil fuel ash (POFA) heat treatment on the strength activity, porosity, and water absorption of cement mortar. The cement mortar mixtures were typically comprising cement or cement in combination with ultrafine treated POFA (u-TPOFA) which is the final form of the treated POFA, sand, water, and a superplasticizer. Before utilizing the u-TPOFA in mortar mixtures, the treatment processes of POFA were undertaken via five steps (drying at 105 ℃, sieving, grinding, heat treatment, re-grinding) to form u-TPOFA. The heat treatment was performed at three different heating temperatures (i.e., 550 ℃, 600 ℃, and 650 ℃). The ratio on mass/mass basis of the blended ordinary Portland cement (OPC) with u-TPOFA was OPC:u-TPOFA of 70%:30%. A total of four mixtures were prepared, consisting of a plain control mixture (designated as PCM) and three mixtures containing 30% of u-TPOFA treated at three different temperatures designated as M1 "550 ℃," M2 "600 ℃," and M3 "650 ℃". The results show that the optimum mixture was M2 which achieved the highest strength activity index (SAI) of 101.84% and 107% among all mixtures at 7 days and 28 days, respectively. Meanwhile, the porosity (P%) and water absorption (Abs%) of M2 exhibited the lowest values of 9.3% and 4.5%, respectively, among all the mixtures at 28 days. This superior performance of u-TPOFA treated at 600 ℃ represented in the M2 mixture was due to the formation of more binding phases consisting of calcium silicate hydrate (C-S-H) type gel originated from a higher pozzolanic reaction and the filler effects caused by the fine u-TPOFA microparticles. These observations were further confirmed by the improved performance of the M2 mix among all the designed mixes which also exhibited better results in terms of bulk density (BD), ultrasonic pulse velocity (UPV), X-ray diffraction (XRD) as well as thermogravimetry (TGA) and field emission scanning electron microscopy (FESEM-EDX) analyses.
MeSH term(s)	Compressive Strength ; Construction Materials/analysis ; Hot Temperature ; Palm Oil ; Porosity ; Water/analysis
Chemical Substances	Water (059QF0KO0R) ; Palm Oil (5QUO05548Z)
Language	English
Publishing date	2022-05-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-20710-3
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Article ; Online: Effects of interventions targeting the systemic inflammatory response to cardiac surgery on clinical outcomes in adults.

Abbasciano, Riccardo Giuseppe / Tomassini, Sara / Roman, Marius A / Rizzello, Angelica / Pathak, Suraj / Ramzi, Joussi / Lucarelli, Carla / Layton, Georgia / Butt, Ayesha / Lai, Florence / Kumar, Tracy / Wozniak, Marcin J / Murphy, Gavin J

The Cochrane database of systematic reviews

2023 Volume 10, Page(s) CD013584

Abstract: Background: Organ injury is a common and severe complication of cardiac surgery that contributes to the majority of deaths. There are no effective treatment or prevention strategies. It has been suggested that innate immune system activation may have a ... ...

Abstract	Background: Organ injury is a common and severe complication of cardiac surgery that contributes to the majority of deaths. There are no effective treatment or prevention strategies. It has been suggested that innate immune system activation may have a causal role in organ injury. A wide range of organ protection interventions targeting the innate immune response have been evaluated in randomised controlled trials (RCTs) in adult cardiac surgery patients, with inconsistent results in terms of effectiveness. Objectives: The aim of the review was to summarise the results of RCTs of organ protection interventions targeting the innate immune response in adult cardiac surgery. The review considered whether the interventions had a treatment effect on inflammation, important clinical outcomes, or both. Search methods: CENTRAL, MEDLINE, Embase, conference proceedings and two trial registers were searched on October 2022 together with reference checking to identify additional studies. Selection criteria: RCTs comparing organ protection interventions targeting the innate immune response versus placebo or no treatment in adult patients undergoing cardiac surgery where the treatment effect on innate immune activation and on clinical outcomes of interest were reported. Data collection and analysis: Searches, study selection, quality assessment, and data extractions were performed independently by pairs of authors. The primary inflammation outcomes were peak IL-6 and IL-8 concentrations in blood post-surgery. The primary clinical outcome was in-hospital or 30-day mortality. Treatment effects were expressed as risk ratios (RR) and standardised mean difference (SMD) with 95% confidence intervals (CI). Meta-analyses were performed using random effects models, and heterogeneity was assessed using I Main results: A total of 40,255 participants from 328 RCTs were included in the synthesis. The effects of treatments on IL-6 (SMD -0.77, 95% CI -0.97 to -0.58, I Authors' conclusions: A systematic review of RCTs of organ protection interventions targeting innate immune system activation did not resolve uncertainty as to the effectiveness of these treatments, or the role of innate immunity in organ injury following cardiac surgery.
MeSH term(s)	Humans ; Adult ; Interleukin-6 ; Interleukin-8 ; Cardiac Surgical Procedures/adverse effects ; Inflammation ; Systemic Inflammatory Response Syndrome
Chemical Substances	Interleukin-6 ; Interleukin-8
Language	English
Publishing date	2023-10-24
Publishing country	England
Document type	Systematic Review ; Journal Article ; Review ; Research Support, Non-U.S. Gov't
ISSN	1469-493X
ISSN (online)	1469-493X
DOI	10.1002/14651858.CD013584.pub2
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Article ; Online: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery.

Hindi, Sajedah M / Petrany, Michael J / Greenfeld, Elena / Focke, Leah C / Cramer, Alyssa A W / Whitt, Michael A / Khairallah, Ramzi J / Ward, Christopher W / Chamberlain, Jeffrey S / Podbilewicz, Benjamin / Prasad, Vikram / Millay, Douglas P

Cell

2023 Volume 186, Issue 10, Page(s) 2062–2077.e17

Abstract: Entry of enveloped viruses into cells is mediated by viral fusogenic proteins that drive membrane rearrangements needed for fusion between viral and target membranes. Skeletal muscle development also requires membrane fusion events between progenitor ... ...

Abstract	Entry of enveloped viruses into cells is mediated by viral fusogenic proteins that drive membrane rearrangements needed for fusion between viral and target membranes. Skeletal muscle development also requires membrane fusion events between progenitor cells to form multinucleated myofibers. Myomaker and Myomerger are muscle-specific cell fusogens but do not structurally or functionally resemble classical viral fusogens. We asked whether the muscle fusogens could functionally substitute for viral fusogens, despite their structural distinctiveness, and fuse viruses to cells. We report that engineering of Myomaker and Myomerger on the membrane of enveloped viruses leads to specific transduction of skeletal muscle. We also demonstrate that locally and systemically injected virions pseudotyped with the muscle fusogens can deliver μDystrophin to skeletal muscle of a mouse model of Duchenne muscular dystrophy and alleviate pathology. Through harnessing the intrinsic properties of myogenic membranes, we establish a platform for delivery of therapeutic material to skeletal muscle.
MeSH term(s)	Animals ; Mice ; Cell Fusion ; Membrane Fusion ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Muscle Development ; Muscle, Skeletal/metabolism ; Muscle, Skeletal/virology ; Bioengineering/methods ; Muscular Dystrophy, Duchenne/therapy ; Disease Models, Animal ; Viral Tropism ; Lentivirus/genetics
Chemical Substances	Membrane Proteins
Language	English
Publishing date	2023-04-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2023.03.033
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Article ; Online: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery.

Hindi, Sajedah M / Petrany, Michael J / Greenfeld, Elena / Focke, Leah C / Cramer, Alyssa A W / Whitt, Michael A / Khairallah, Ramzi J / Ward, Christopher W / Chamberlain, Jeffrey S / Prasad, Vikram / Podbilewicz, Benjamin / Millay, Douglas P

Cell

2023 Volume 186, Issue 16, Page(s) 3520

Language	English
Publishing date	2023-08-05
Publishing country	United States
Document type	Published Erratum
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2023.06.025
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Article ; Online: Depletion of skeletal muscle satellite cells attenuates pathology in muscular dystrophy

Justin G. Boyer / Jiuzhou Huo / Sarah Han / Julian R. Havens / Vikram Prasad / Brian L. Lin / David A. Kass / Taejeong Song / Sakthivel Sadayappan / Ramzi J. Khairallah / Christopher W. Ward / Jeffery D. Molkentin

Nature Communications, Vol 13, Iss 1, Pp 1-

2022 Volume 10

Abstract: Boyer et al. created genetic mouse models of muscular dystrophy in which satellite cells were selectively depleted. The depletion of satellite cells at select times was protective. Myofibers no longer had plasma membrane instability leading to tissue ... ...

Abstract	Boyer et al. created genetic mouse models of muscular dystrophy in which satellite cells were selectively depleted. The depletion of satellite cells at select times was protective. Myofibers no longer had plasma membrane instability leading to tissue wasting in the muscular dystrophies.
Keywords	Science ; Q
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: In vitro

Gould, Nicole R / Leser, Jenna M / Torre, Olivia M / Khairallah, Ramzi J / Ward, Christopher W / Stains, Joseph P

Bio-protocol

2021 Volume 11, Issue 23, Page(s) e4251

Abstract: Bone is a dynamic tissue that adapts to changes in its mechanical environment. Mechanical stimuli pressurize interstitial fluid in the lacunar-canalicular system within the bone matrix, causing fluid shear stress (FSS) across bone embedded, mechano- ... ...

Abstract	Bone is a dynamic tissue that adapts to changes in its mechanical environment. Mechanical stimuli pressurize interstitial fluid in the lacunar-canalicular system within the bone matrix, causing fluid shear stress (FSS) across bone embedded, mechano-sensitive osteocytes. Therefore, modeling this mechanical stimulus
Language	English
Publishing date	2021-12-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2833269-6
ISSN	2331-8325 ; 2331-8325
ISSN (online)	2331-8325
ISSN	2331-8325
DOI	10.21769/BioProtoc.4251
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Article ; Online: Finding the missing link: disulfide-containing proteins via a high-throughput proteomics approach.

Khairallah, Ramzi J / Sadayappan, Sakthivel

Proteomics

2013 Volume 13, Issue 22, Page(s) 3245–3246

Abstract: Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on ...

Abstract	Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on complex and time-consuming protein purification and reduction reactions to yield useful information. In this issue of Proteomics, Zhao et al. (high-throughput screening of disulfide-containing proteins in a complex mixture, Proteomics 2013, 13, 3256-3260) devise a clever and rapid method for high-throughput determination of disulfides in proteins via reduction by tris(2-carboxyethyl)phosphine. Their work provides the foundation necessary to undertake more complex experiments in biological samples.
MeSH term(s)	Disulfides/analysis ; High-Throughput Screening Assays/methods ; Proteins/chemistry
Chemical Substances	Disulfides ; Proteins
Language	English
Publishing date	2013-10-21
Publishing country	Germany
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Comment
ZDB-ID	2032093-0
ISSN	1615-9861 ; 1615-9853
ISSN (online)	1615-9861
ISSN	1615-9853
DOI	10.1002/pmic.201300445
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Zs.A 5386: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Depletion of skeletal muscle satellite cells attenuates pathology in muscular dystrophy.

Boyer, Justin G / Huo, Jiuzhou / Han, Sarah / Havens, Julian R / Prasad, Vikram / Lin, Brian L / Kass, David A / Song, Taejeong / Sadayappan, Sakthivel / Khairallah, Ramzi J / Ward, Christopher W / Molkentin, Jeffery D

Nature communications

2022 Volume 13, Issue 1, Page(s) 2940

Abstract: Skeletal muscle can repair and regenerate due to resident stem cells known as satellite cells. The muscular dystrophies are progressive muscle wasting diseases underscored by chronic muscle damage that is continually repaired by satellite cell-driven ... ...

Abstract	Skeletal muscle can repair and regenerate due to resident stem cells known as satellite cells. The muscular dystrophies are progressive muscle wasting diseases underscored by chronic muscle damage that is continually repaired by satellite cell-driven regeneration. Here we generate a genetic strategy to mediate satellite cell ablation in dystrophic mouse models to investigate how satellite cells impact disease trajectory. Unexpectedly, we observe that depletion of satellite cells reduces dystrophic disease features, with improved histopathology, enhanced sarcolemmal stability and augmented muscle performance. Mechanistically, we demonstrate that satellite cells initiate expression of the myogenic transcription factor MyoD, which then induces re-expression of fetal genes in the myofibers that destabilize the sarcolemma. Indeed, MyoD re-expression in wildtype adult skeletal muscle reduces membrane stability and promotes histopathology, while MyoD inhibition in a mouse model of muscular dystrophy improved membrane stability. Taken together these observations suggest that satellite cell activation and the fetal gene program is maladaptive in chronic dystrophic skeletal muscle.
MeSH term(s)	Animals ; Disease Models, Animal ; Mice ; Muscle Development ; Muscle, Skeletal/metabolism ; Muscular Dystrophies/metabolism ; Satellite Cells, Skeletal Muscle/metabolism ; Stem Cells
Language	English
Publishing date	2022-05-26
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-022-30619-7
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