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  1. Article ; Online: Malignant pericardial effusion complicated by cardiac tamponade under atezolizumab

    Lardinois Benjamin / Goeminne Jean-Charles / Miller Laurence / Randazzo Adrien / Laurent Terry / Debois Régis

    SAGE Open Medical Case Reports, Vol

    2021  Volume 9

    Abstract: Immune-related adverse events including cardiac toxicity are increasingly described in patients receiving immune checkpoint inhibitors. We described a malignant pericardial effusion complicated by a cardiac tamponade in an advanced non-small cell lung ... ...

    Abstract Immune-related adverse events including cardiac toxicity are increasingly described in patients receiving immune checkpoint inhibitors. We described a malignant pericardial effusion complicated by a cardiac tamponade in an advanced non-small cell lung cancer patient who had received five infusions of atezolizumab, a PDL-1 monoclonal antibody, in combination with cabozantinib. The definitive diagnosis was quickly made by cytology examination showing typical cell abnormalities and high fluorescence cell information provided by the hematology analyzer. The administration of atezolizumab and cabozantinib was temporarily discontinued due to cardiogenic hepatic failure following cardiac tamponade. After the re-initiation of the treatment, pericardial effusion relapsed. In this patient, the analysis of the pericardial fluid led to the final diagnosis of pericardial tumor progression. This was afterwards confirmed by the finding of proliferating intrapericardial tissue by computed tomography scan and ultrasound. This report emphasizes the value of cytology analysis performed in a hematology laboratory as an accurate and immediate tool for malignancy detection in pericardial effusions.
    Keywords Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher SAGE Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Leptomeningeal Carcinomatosis: A Call for Optimizing Diagnostic Sensitivity by the Hematology Laboratory.

    Lardinois, Benjamin / Miller, Laurence / Randazzo, Adrien / Laurent, Terry / Debois, Régis / Henry, Stéphanie

    Case reports in oncology

    2021  Volume 14, Issue 2, Page(s) 1248–1253

    Abstract: In the cerebrospinal fluid (CSF), the demonstration of malignant cells by cytological examination is currently the gold standard for the diagnosis of leptomeningeal carcinomatosis (LC). However, a positive cytology is observed in only 50-60% of patients ... ...

    Abstract In the cerebrospinal fluid (CSF), the demonstration of malignant cells by cytological examination is currently the gold standard for the diagnosis of leptomeningeal carcinomatosis (LC). However, a positive cytology is observed in only 50-60% of patients with LC and highly dependent on pre-analytical factors. The hematology laboratory could provide an immediate and accurate diagnosis, but diagnostic sensitivity is not always optimized once the sample is received. We hereby report a 49-year-old woman with a 3-year grade III invasive ductal carcinoma who was admitted to the emergency department due to headaches, nausea, and vomiting. The CSF revealed pleocytosis with suspicious high fluorescent cells on the hematology analyzer concomitantly with biochemical alterations. Cytomorphological examination confirmed tumor cells, thus diagnosing a leptomeningeal metastasis of her breast cancer. The patient was eventually transferred to palliative care. Cytological examination is a valuable tool for a rapid diagnosis of LC if diagnostic performance is optimized. In addition to repeated CSF collections with a sufficient volume (5-10 mL), this could be reached by processing the CSF as soon as possible, taking into account the fluorescence information from the analyzer, proceeding systematically to microscopic examination even with normal CSF white blood cell count, and providing quality improvement of the staff to identify malignant cells.
    Language English
    Publishing date 2021-08-18
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2458961-5
    ISSN 1662-6575
    ISSN 1662-6575
    DOI 10.1159/000518314
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  3. Article: A Clostridium hathewayi isolate in blood culture of a patient with an acute appendicitis

    Randazzo, Adrien / Anne Kornreich / Bénédicte Lissoir

    Anaerobe. 2015 Oct., v. 35

    2015  

    Abstract: Clostridium species is a group of anaerobic bacteria constituting the colonic microflora of the intestinal tract. Since molecular methodologies based on 16 rRNA have been established for the classification and the recognition of bacterial species, more ... ...

    Abstract Clostridium species is a group of anaerobic bacteria constituting the colonic microflora of the intestinal tract. Since molecular methodologies based on 16 rRNA have been established for the classification and the recognition of bacterial species, more than 150 species of Clostridium have been described. Most are considered harmless saprophytes; however, these bacteria may be involved in a wide variety of infections and may be a common cause of enteritis and enterotoxemias in humans.We present the case of a 60-year-old Asian patient admitted in the emergency room with an acute appendicitis where a blood culture showed the presence of a Clostridium hathewayi. This microorganism is an anaerobic bacteria described in 2001 as a Gram negative end-pointed bacillus, usually endospore-forming. It was reclassified in 2014 as Hungatella hathewayi. A literature review has been performed to find articles relating to this bacteria in a clinical case.C. hathewayi is microorganism recently reclassified as Hungatella hathewayi. Its growth in blood cultures has been reported in a few cases in the literature. Although only a few articles have reported its involvement in clinical infections, we assess that its part in the cause of the illness should be evaluated.
    Keywords anaerobes ; bacteria ; blood ; Clostridium ; enteritis ; intestinal microorganisms ; intestines ; patients ; ribosomal RNA ; saprophytes
    Language English
    Dates of publication 2015-10
    Size p. 44-47.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1237621-8
    ISSN 1075-9964
    ISSN 1075-9964
    DOI 10.1016/j.anaerobe.2015.07.003
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4318: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  4. Article ; Online: A Clostridium hathewayi isolate in blood culture of a patient with an acute appendicitis.

    Randazzo, Adrien / Kornreich, Anne / Lissoir, Bénédicte

    Anaerobe

    2015  Volume 35, Issue Pt B, Page(s) 44–47

    Abstract: Introduction: Clostridium species is a group of anaerobic bacteria constituting the colonic microflora of the intestinal tract. Since molecular methodologies based on 16 rRNA have been established for the classification and the recognition of bacterial ... ...

    Abstract Introduction: Clostridium species is a group of anaerobic bacteria constituting the colonic microflora of the intestinal tract. Since molecular methodologies based on 16 rRNA have been established for the classification and the recognition of bacterial species, more than 150 species of Clostridium have been described. Most are considered harmless saprophytes; however, these bacteria may be involved in a wide variety of infections and may be a common cause of enteritis and enterotoxemias in humans.
    Case presentation: We present the case of a 60-year-old Asian patient admitted in the emergency room with an acute appendicitis where a blood culture showed the presence of a Clostridium hathewayi. This microorganism is an anaerobic bacteria described in 2001 as a Gram negative end-pointed bacillus, usually endospore-forming. It was reclassified in 2014 as Hungatella hathewayi. A literature review has been performed to find articles relating to this bacteria in a clinical case.
    Conclusion: C. hathewayi is microorganism recently reclassified as Hungatella hathewayi. Its growth in blood cultures has been reported in a few cases in the literature. Although only a few articles have reported its involvement in clinical infections, we assess that its part in the cause of the illness should be evaluated.
    MeSH term(s) Appendicitis/complications ; Asian Continental Ancestry Group ; Bacteremia/diagnosis ; Bacteremia/microbiology ; Blood/microbiology ; Clostridiales/isolation & purification ; Gram-Positive Bacterial Infections/diagnosis ; Gram-Positive Bacterial Infections/microbiology ; Humans ; Male ; Middle Aged
    Language English
    Publishing date 2015-10
    Publishing country England
    Document type Case Reports ; Journal Article ; Review
    ZDB-ID 1237621-8
    ISSN 1095-8274 ; 1075-9964
    ISSN (online) 1095-8274
    ISSN 1075-9964
    DOI 10.1016/j.anaerobe.2015.07.003
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4318: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Optimal turnaround time for direct identification of microorganisms by mass spectrometry in blood culture.

    Randazzo, Adrien / Simon, Marc / Goffinet, Pierre / Classen, Jean-François / Hougardy, Nicolas / Pierre, Pascal / Kinzinger, Philippe / Mauel, Etienne / Goffinet, Jean-Sébastien

    Journal of microbiological methods

    2016  Volume 130, Page(s) 1–5

    Abstract: Introduction: During the past few years, several studies describing direct identification of bacteria from blood culture using mass spectrometry have been published. These methods cannot, however, be easily integrated into a common laboratory workflow ... ...

    Abstract Introduction: During the past few years, several studies describing direct identification of bacteria from blood culture using mass spectrometry have been published. These methods cannot, however, be easily integrated into a common laboratory workflow because of the high hands-on time they require. In this paper, we propose a new method of identification with a short hands-on time and a turnaround time shorter than 15min.
    Materials and methods: Positive blood bottles were homogenised and 600μL of blood were transferred to an Eppendorf tube where 600μL of lysis buffer were added. After homogenisation, a centrifugation step of 4min at 10,500g was performed and the supernatant was discarded. The pellet was then washed and loaded in quadruplicate into wells of a Vitek® MS-DS plate. Each well was covered with a saturated matrix solution and a MALDI-TOF mass spectrometry analysis was performed. Species were identified using the software Myla 3.2.0-2.
    Results: We analysed 266 positive blood culture bottles. A microorganism grew in 261 cultures, while five bottles remained sterile after 48h of incubation in subculture. Our method reaches a probability of detection at the species level of 77.8% (203/261) with a positive predictive value of 99.5% (202/203).
    Conclusion: We developed a new method for the identification of microorganisms using mass spectrometry, directly performed from a positive blood culture. This method has short hands-on time and turnaround time and can easily take place in the workflow of a laboratory, with comparable results in performance with other methods reported in the literature.
    MeSH term(s) Bacteria/classification ; Bacteria/growth & development ; Bacteria/isolation & purification ; Bacterial Infections/blood ; Bacterial Infections/microbiology ; Bacteriological Techniques/methods ; Blood/microbiology ; Blood Culture/methods ; Cell Culture Techniques ; Culture Media ; Laboratories ; Mass Spectrometry/methods ; Probability ; Sensitivity and Specificity ; Software ; Time Factors
    Chemical Substances Culture Media
    Language English
    Publishing date 2016-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2016.08.019
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  6. Article: Optimal turnaround time for direct identification of microorganisms by mass spectrometry in blood culture

    Randazzo, Adrien / Etienne Mauel / Jean-François Classen / Jean-Sébastien Goffinet / Marc Simon / Nicolas Hougardy / Pascal Pierre / Philippe Kinzinger / Pierre Goffinet

    Journal of microbiological methods. 2016 Nov., v. 130

    2016  

    Abstract: During the past few years, several studies describing direct identification of bacteria from blood culture using mass spectrometry have been published.These methods cannot, however, be easily integrated into a common laboratory workflow because of the ... ...

    Abstract During the past few years, several studies describing direct identification of bacteria from blood culture using mass spectrometry have been published.These methods cannot, however, be easily integrated into a common laboratory workflow because of the high hands-on time they require. In this paper, we propose a new method of identification with a short hands-on time and a turnaround time shorter than 15min.Positive blood bottles were homogenised and 600μL of blood were transferred to an Eppendorf tube where 600μL of lysis buffer were added. After homogenisation, a centrifugation step of 4min at 10,500g was performed and the supernatant was discarded. The pellet was then washed and loaded in quadruplicate into wells of a Vitek® MS-DS plate. Each well was covered with a saturated matrix solution and a MALDI-TOF mass spectrometry analysis was performed. Species were identified using the software Myla 3.2.0-2.We analysed 266 positive blood culture bottles. A microorganism grew in 261 cultures, while five bottles remained sterile after 48h of incubation in subculture. Our method reaches a probability of detection at the species level of 77.8% (203/261) with a positive predictive value of 99.5% (202/203).We developed a new method for the identification of microorganisms using mass spectrometry, directly performed from a positive blood culture. This method has short hands-on time and turnaround time and can easily take place in the workflow of a laboratory, with comparable results in performance with other methods reported in the literature.
    Keywords bacteria ; blood ; bottles ; centrifugation ; computer software ; homogenization ; matrix-assisted laser desorption-ionization mass spectrometry ; probability
    Language English
    Dates of publication 2016-11
    Size p. 1-5.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2016.08.019
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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