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  1. Article: Novel Osteo Refurbishment Method and Choice of Colouring Materials for Anatomy Specimens.

    Borthakur, Dibakar / Kumar, Rajesh / Rani, Neerja / Dada, Rima

    Medeniyet medical journal

    2023  Volume 38, Issue 1, Page(s) 54–62

    Abstract: Objective: The introduction of competency-based medical education curriculum and a shifted focus towards self-directed learning (SDL) in medical schools across the world has necessitated a reduction of didactic teaching hours. Museum specimens are an ... ...

    Abstract Objective: The introduction of competency-based medical education curriculum and a shifted focus towards self-directed learning (SDL) in medical schools across the world has necessitated a reduction of didactic teaching hours. Museum specimens are an indispensable tool in anatomy teaching and can play a pivotal role in fostering and reinforcing SDL. Coloring of specimens makes it attractive and facilitates understanding anatomy. The aims of this study were: (1) weigh up the two commonly used coloring materials in anatomy viz. acrylic paint and nail polish; and (2) to assess the perception of anatomy students towards a Novel Osteo Refurbishment Method (NORM) for restoration of partially damaged osteology specimens.
    Methods: Undergraduate and postgraduate students of anatomy were recruited in the study. Participants responded to a 5-point Likert scale questionnaire. Obtained data were analyzed using Microsoft Excel version 2019. For a comparison of the two coloring agents Mann-Whitney U test was applied to the responses toward the Likert scale-based questionnaire. P-value <0.05 was considered significant.
    Results: Out of 318 study participants, 63.7% and 36.3% of the respondents were male and female, respectively. 90.5 % were new entrants to university and the remainder 9.5% had a prior university degree. Students found the acrylic paint to be a better coloring agent and they also opined that NORM generated refurbished osteology specimens could serve as a useful self-learning tool. Acrylic paint was found to be more suitable as compared to nail polish with significant p-value in most of the parameters tested.
    Conclusions: Acrylic colour holds a good place in coloring both specimens and models in anatomy. Nail polish can be an alternative but with some noted disadvantages. The initial experience with NORM yielded encouraging results with potential practical utility.
    Language English
    Publishing date 2023-03-22
    Publishing country Turkey
    Document type Journal Article
    ZDB-ID 3035195-9
    ISSN 2149-4606 ; 2149-2042
    ISSN (online) 2149-4606
    ISSN 2149-2042
    DOI 10.4274/MMJ.galenos.2023.38275
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A rare case of multiple visceral vascular variations around the kidneys: morphological and clinical aspects.

    Borthakur, Dibakar / Ansari, Mohammed Ahmed / Rani, Neerja / Kumar, Rajesh / Baxla, Monica

    Jornal vascular brasileiro

    2024  Volume 23, Page(s) e20230120

    Abstract: Knowledge of the anatomical variations of the visceral branches of the abdominal aorta is important information for planning any surgeries in the region. We present here a rare constellation of variations of visceral vessels around the kidneys with a ... ...

    Abstract Knowledge of the anatomical variations of the visceral branches of the abdominal aorta is important information for planning any surgeries in the region. We present here a rare constellation of variations of visceral vessels around the kidneys with a brief review of the recent literature. On the right side, an accessory renal artery was observed originating just distal to the main renal artery. The middle suprarenal artery was absent on the right side and there were two inferior suprarenal arteries originating from a branch of the main right renal artery. On the left side, the testicular artery had an arched course anterior to the left renal vein mimicking an unusual variety of nutcracker phenomenon. The right kidney was drained by two renal veins into the inferior vena cava. Knowledge of the coexistence of such complex anatomical variations might be helpful for clinicians during diagnostic and therapeutic procedures.
    Language English
    Publishing date 2024-02-12
    Publishing country Brazil
    Document type Case Reports
    ZDB-ID 2571403-X
    ISSN 1677-7301 ; 1677-7301
    ISSN (online) 1677-7301
    ISSN 1677-7301
    DOI 10.1590/1677-5449.202301202
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A tale of exosomes and their implication in cancer.

    Mishra, Abhay / Bharti, Prahalad Singh / Rani, Neerja / Nikolajeff, Fredrik / Kumar, Saroj

    Biochimica et biophysica acta. Reviews on cancer

    2023  Volume 1878, Issue 4, Page(s) 188908

    Abstract: Cancer is a cause of high deaths worldwide and also a huge burden for the health system. Cancer cells have unique properties such as a high rate of proliferation, self-renewal, metastasis, and treatment resistance, therefore, the development of novel ... ...

    Abstract Cancer is a cause of high deaths worldwide and also a huge burden for the health system. Cancer cells have unique properties such as a high rate of proliferation, self-renewal, metastasis, and treatment resistance, therefore, the development of novel diagnoses of cancers is a tedious task. Exosomes are secreted by virtually all cell types and have the ability to carry a multitude of biomolecules crucial for intercellular communication, hence, contributing a crucial part in the onset and spread of cancer. These exosomal components can be utilized in the development of markers for diagnostic and prognostic purposes for various cancers. This review emphasized primarily the following topics: exosomes structure and functions, isolation and characterization strategies of exosomes, the role of exosomal contents in cancer with a focus in particular on noncoding RNA and protein, exosomes, and the cancer microenvironment interactions, cancer stem cells, and tumor diagnosis and prognosis based on exosomes.
    MeSH term(s) Humans ; Exosomes/metabolism ; Neoplasms/pathology ; MicroRNAs/genetics ; Cell Communication ; RNA, Long Noncoding/genetics ; Tumor Microenvironment/genetics
    Chemical Substances MicroRNAs ; RNA, Long Noncoding
    Language English
    Publishing date 2023-05-10
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2918802-7
    ISSN 1879-2561 ; 0304-419X
    ISSN (online) 1879-2561
    ISSN 0304-419X
    DOI 10.1016/j.bbcan.2023.188908
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Bilateral absence of subclavius muscles with thickened costocoracoid ligaments: a case report with the clinical-anatomical correlation.

    Dheeraj, Kasapuram / Sudheer, Harisha K / Bhukiya, Subhash / Rani, Neerja / Singh, Seema

    Anatomy & cell biology

    2022  Volume 55, Issue 2, Page(s) 255–258

    Abstract: Anatomical variation is defined as normal flexibility in the topography and morphology of body structures. Such variations are not uncommon in muscles, particularly in the upper limb. Subclavius muscle (SM) has a proximal attachment to the first ... ...

    Abstract Anatomical variation is defined as normal flexibility in the topography and morphology of body structures. Such variations are not uncommon in muscles, particularly in the upper limb. Subclavius muscle (SM) has a proximal attachment to the first costochondral junction and the muscle fibers are then directed upwards and laterally to get attached distally to the subclavian groove of the clavicle. Having similar attachments as the subclavius, the costocoracoid ligament (CCL) is the thickening of the proximal part of clavipectoral fascia extending up to the coracoid process. Both SM and CCL help in the maintenance of smooth movements of the pectoral girdle and both may not always be present. Absent SM may be due to anomalous development from the muscle matrix that also forms the inferior belly of the omohyoid apart from the SMs. This anatomical variation may be associated with thickened CCLs and can be correlated to the smooth functioning of the pectoral girdle.
    Language English
    Publishing date 2022-06-28
    Publishing country Korea (South)
    Document type Case Reports
    ZDB-ID 2590173-4
    ISSN 2093-3673 ; 2093-3665
    ISSN (online) 2093-3673
    ISSN 2093-3665
    DOI 10.5115/acb.21.246
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Is Asterion a Reliable Surgical Landmark for the Transverse and Sigmoid Sinus Junction in Indian Skulls?

    Rohilla, Swati / Singh, Seema / Sudheer Arava, Harisha Kusuma / Tamang, Shradha / Rani, Neerja / Gurjar, Hitesh Kumar / Shariff, Ahmadulla

    Neurology India

    2023  Volume 71, Issue 4, Page(s) 732–736

    Abstract: Background: Asterion is the junction of lambdoid, parietomastoid, and occipitomastoid sutures. In traditional anatomy teaching, it is believed that asterion sits over the transverse-sigmoid sinus junction (TSSJ). It is a significant surgical landmark ... ...

    Abstract Background: Asterion is the junction of lambdoid, parietomastoid, and occipitomastoid sutures. In traditional anatomy teaching, it is believed that asterion sits over the transverse-sigmoid sinus junction (TSSJ). It is a significant surgical landmark for the placement of a burr hole in the retrosigmoid approach.
    Objective: The purpose of this study was to evaluate the asterion's accuracy as a TSSJ-specific external surgical landmark in the Indian population.
    Materials and methods: 16 human dried skulls were obtained from the Department of Anatomy, AIIMS, New Delhi. The point of TSSJ was taken between the two lips and just posterior to the entry of the superior petrosal sinus. Asterion internally was localized with the help of a geometrical divider. The x- horizontal/ anterior and y- vertically superior (+)/ inferior (-) distances were measured from asterion (internally) to the TSSJ by a digital vernier caliper.
    Results: The mean horizontal distance (x) of the left side asterion & TSSJ was 10.3±1.0mm whereas the vertical distance (y) ranged between +2 to -4.3 mm. The mean horizontal distance (x) of the right side asterion & TSSJ was 13.5±1.4mm whereas the vertical distance (y) ranged between +3 to -4.2 mm.
    Conclusion: Asterion is not a reliable landmark for TSSJ in Indian skulls. The TSSJ with respect to asterion was found on average 10mm and 13.5mm anterior on the left and right side respectively, and mostly inferior (average 4.2mm) in 75% of the skulls. The TSSJ was closer to the asterion on the left side in comparison with the right side. However, further studies with a larger sample size will be needed to evaluate the population-specific relation of asterion with the TSSJ.
    MeSH term(s) Humans ; Craniotomy/methods ; Skull/surgery ; Cranial Sinuses/surgery ; Cranial Sutures/anatomy & histology ; Transverse Sinuses/surgery
    Language English
    Publishing date 2023-08-27
    Publishing country India
    Document type Journal Article
    ZDB-ID 415522-1
    ISSN 1998-4022 ; 0028-3886
    ISSN (online) 1998-4022
    ISSN 0028-3886
    DOI 10.4103/0028-3886.383854
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Atlanto-occipital assimilation: embryological basis and its clinical significance.

    Hanusun N, Hari Hara / Singh, Akanksha / Poddar, Pooja / J P, Jessy / Rani, Neerja / Gurjar, Hitesh / Singh, Seema

    Anatomy & cell biology

    2023  Volume 57, Issue 1, Page(s) 147–151

    Abstract: Atlanto-occipital assimilation is an osseous embryological anomaly of the craniovertebral junction in which the atlas (C1) is fused to the occiput of skull. Embryologically, this assimilation may happen due to failure of the segmentation and separation ... ...

    Abstract Atlanto-occipital assimilation is an osseous embryological anomaly of the craniovertebral junction in which the atlas (C1) is fused to the occiput of skull. Embryologically, this assimilation may happen due to failure of the segmentation and separation of the caudal occipital and the cranial cervical sclerotome. The segmentation clock is maintained by NOTCH and WNT signalling pathways along with
    Language English
    Publishing date 2023-12-20
    Publishing country Korea (South)
    Document type Case Reports
    ZDB-ID 2590173-4
    ISSN 2093-3673 ; 2093-3665
    ISSN (online) 2093-3673
    ISSN 2093-3665
    DOI 10.5115/acb.23.225
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Hydrogen sulfide promotes migration of trophoblast cells by a Rho GTPase mediated actin cytoskeleton reorganization.

    Gupta, Sunil Kumar / Mochan, Sankat / Arora, Pallavi / Rani, Neerja / Luthra, Kalpana / Dwivedi, Sadanand / Bhatla, Neerja / Kshetrapal, Pallavi / Dhingra, Renu

    Placenta

    2023  Volume 142, Page(s) 135–146

    Abstract: Introduction: Preeclampsia (PE) arises due to defective spiral artery remodelling which may be due to deficient migration of trophoblast cells. Migration of human endothelial cells has been shown to be promoted via Hydrogen sulphide(H: Methods: ... ...

    Abstract Introduction: Preeclampsia (PE) arises due to defective spiral artery remodelling which may be due to deficient migration of trophoblast cells. Migration of human endothelial cells has been shown to be promoted via Hydrogen sulphide(H
    Methods: Placental tissues were obtained post-delivery from consented preeclamptic and normotensive mothers (n = 60). The protein expression levels of cystathionine-gamma-lyase (CSE) and cystathionine-beta-synthase (CBS) along with its downstream migratory molecules were compared in both the arms. The pro-migratory role of H
    Results: H
    Discussion: Exogenous H
    Language English
    Publishing date 2023-09-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 603951-0
    ISSN 1532-3102 ; 0143-4004
    ISSN (online) 1532-3102
    ISSN 0143-4004
    DOI 10.1016/j.placenta.2023.09.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Deciphering pancreatic neuroendocrine tumors: Unveiling through circulating small extracellular vesicles.

    Gorai, Priya Kumari / Rastogi, Simran / Bharti, Prahalad Singh / Agarwal, Shipra / Pal, Sujoy / Sharma, Mehar Chand / Kumar, Rakesh / Nikolajeff, Fredrik / Kumar, Saroj / Rani, Neerja

    Heliyon

    2024  Volume 10, Issue 7, Page(s) e29079

    Abstract: The survival rate over a five-year period for rare pancreatic neuroendocrine tumors (PanNET) is notably lower compared to other neuroendocrine tumors due to late-stage detection, which is a consequence of the absence of suitable diagnostic markers; ... ...

    Abstract The survival rate over a five-year period for rare pancreatic neuroendocrine tumors (PanNET) is notably lower compared to other neuroendocrine tumors due to late-stage detection, which is a consequence of the absence of suitable diagnostic markers; therefore, there exists a critical need for an early-stage biomarker-specific to PanNETs. This study introduces a novel approach, investigating the impact of small extracellular vesicles (sEV) in PanNET growth and metastasis. As proof of concept, this study shows a correlation between sEV concentration in controls and PanNET. Notably, higher sEV concentrations were observed in PanNETs than in controls (p < 0.0001) with a sensitivity of 100%. Further, apparent differences were observed in the sEV concentrations between controls and grades 1 PanNET (p = 0.005). The expression of sEV markers was confirmed using CD63, TSG101, CD9, Flotillin-1, and GAD65 antibodies. Additionally, the expression of cancer marker BIRC2/cIAP1 (p = 0.002) and autophagy marker Beclin-1 (p = 0.02) were observed in plasma-derived sEVs and PanNET tissue. This study represents the first to indicate the increased secretion of sEV in PanNET patients' blood plasma, proposing potential function of sEV as a new biomarker for early-stage PanNET detection.
    Language English
    Publishing date 2024-04-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e29079
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: C1QA and COMP: plasma-based biomarkers for early diagnosis of pancreatic neuroendocrine tumors.

    Gorai, Priya Kumari / Bharti, Prahalad Singh / Kumar, Shashi / Rajacharya, Girish H / Bandyopadhyay, Sabyasachi / Pal, Sujoy / Dhingra, Renu / Kumar, Rakesh / Nikolajeff, Fredrik / Kumar, Saroj / Rani, Neerja

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 21021

    Abstract: Pancreatic Neuroendocrine tumors (PanNET) are challenging to diagnose and often detected at advanced stages due to a lack of specific and sensitive biomarkers. This study utilized proteomics as a valuable approach for cancer biomarker discovery; ... ...

    Abstract Pancreatic Neuroendocrine tumors (PanNET) are challenging to diagnose and often detected at advanced stages due to a lack of specific and sensitive biomarkers. This study utilized proteomics as a valuable approach for cancer biomarker discovery; therefore, mass spectrometry-based proteomic profiling was conducted on plasma samples from 12 subjects (3 controls; 5 Grade I, 4 Grade II PanNET patients) to identify potential proteins capable of effectively distinguishing PanNET from healthy controls. Data are available via ProteomeXchange with the identifier PXD045045. 13.2% of proteins were uniquely identified in PanNET, while 60% were commonly expressed in PanNET and controls. 17 proteins exhibiting significant differential expression between PanNET and controls were identified with downstream analysis. Further, 5 proteins (C1QA, COMP, HSP90B1, ITGA2B, and FN1) were selected by pathway analysis and were validated using Western blot analysis. Significant downregulation of C1QA (p = 0.001: within groups, 0.03: control vs. grade I, 0.0013: grade I vs. grade II) and COMP (p = 0.011: within groups, 0.019: control vs grade I) were observed in PanNET Grade I & II than in controls. Subsequently, ELISA on 38 samples revealed significant downregulation of C1QA and COMP with increasing disease severity. This study shows the potential of C1QA and COMP in the early detection of PanNET, highlighting their role in the search for early-stage (Grade-I and Grade-II) diagnostic markers and therapeutic targets for PanNET.
    MeSH term(s) Humans ; Neuroendocrine Tumors/pathology ; Pancreatic Neoplasms/pathology ; Proteomics ; Early Detection of Cancer ; Biomarkers, Tumor/analysis
    Chemical Substances Biomarkers, Tumor
    Language English
    Publishing date 2023-11-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-48323-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Vascular foramina of talus: an anatomical study with reference to surgical dissection.

    Vani, P C / Arthi, G / Jessy, J P / Rani, Neerja / Jhajhria, Saroj Kaler

    Surgical and radiologic anatomy : SRA

    2019  Volume 42, Issue 6, Page(s) 685–690

    Abstract: Purpose: To study the distribution and morphometry of vascular foramina of adult human talus in Indian population.: Methods: The study was carried out by using 56 adult human tali. The location, size, number and foraminal index of vascular foramina ... ...

    Abstract Purpose: To study the distribution and morphometry of vascular foramina of adult human talus in Indian population.
    Methods: The study was carried out by using 56 adult human tali. The location, size, number and foraminal index of vascular foramina on head, neck and body of each bone were examined macroscopically. The difference in location of vascular foramina was further studied in relation to the pattern of calcaneal articular facets on talus.
    Results: The vascular foramina were present on the superior neck, inferior neck and medial surface of talar body in all (100%) the bones. The Kruskal-Wallis test followed by series of Mann-Whitney test for post hoc analysis showed the number of vascular foramina was significantly greater on inferior surface of neck and medial surface of body. The number of vascular foramina ranged from 0 to 25. About 77.05% of foramina were ≥ 0.5 mm in size. The mean foraminal index of the closest foramina on inferior surface of neck and medial surface of body was 47.90% and 37.23%, respectively. The mean foraminal index of the farthest foramina on inferior surface of neck and medial surface of body was 75.08% and 71.35%, respectively.
    Conclusion: The present study has provided additional information on the vascular foramina of tali. This knowledge is important to the orthopedic, vascular and podiatric surgeons while performing the surgeries of hind foot. We opine that the lateral approach would be more beneficial in the surgical procedures to talus.
    MeSH term(s) Blood Loss, Surgical/prevention & control ; Calcaneus/anatomy & histology ; Dissection/adverse effects ; Dissection/methods ; Humans ; India ; Orthopedic Procedures/adverse effects ; Orthopedic Procedures/methods ; Talus/blood supply ; Talus/surgery
    Language English
    Publishing date 2019-12-21
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 632839-8
    ISSN 1279-8517 ; 0930-312X ; 0930-1038
    ISSN (online) 1279-8517
    ISSN 0930-312X ; 0930-1038
    DOI 10.1007/s00276-019-02394-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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