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  1. Book: Cytotoxic T-cells

    Gigante, Margherita / Ranieri, Elena

    methods and protocols

    (Methods in molecular biology ; 2325 ; Springer protocols)

    2021  

    Author's details edited by Margherita Gigante and Elena Ranieri
    Series title Methods in molecular biology ; 2325
    Springer protocols
    Collection
    Keywords Killer cells ; T cells
    Subject code 571.966
    Language English
    Size x, 247 Seiten, Illustrationen, 26 cm
    Edition Second edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    Note Includes bibliographical references
    HBZ-ID HT020944324
    ISBN 978-1-0716-1506-5 ; 9781071615072 ; 1-0716-1506-8 ; 1071615076
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -2325- verfügbar in Königswinter Königswinter

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  2. Book: Cytotoxic T-cells

    Ranieri, Elena

    methods and protocols

    (Methods in molecular biology ; 1186 ; Springer protocols)

    2014  

    Author's details ed. by Elena Ranieri
    Series title Methods in molecular biology ; 1186
    Springer protocols
    Collection
    Keywords Killer cells ; T cells
    Subject code 571.966
    Language English
    Size XII, 301 S. : Ill., graph. Darst., 27 cm
    Publisher Humana Press
    Publishing place New York
    Publishing country United States
    Document type Book
    Note Formerly CIP. ; Includes bibliographical references and index
    HBZ-ID HT018418556
    ISBN 978-1-4939-1157-8 ; 9781493911585 ; 1-4939-1157-0 ; 1493911589
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1186- verfügbar in Königswinter Königswinter

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  3. Article ; Online: Phenotypical and Functional Characterization of Cytotoxic Unconventional T-Cells.

    Gigante, Margherita / Ranieri, Elena

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2325, Page(s) 29–39

    Abstract: During the last two decades, the immunology field has been focused on the study of conventional T-cells, leading to important advances in identification of specific subsets involved in promoting and suppressing immune response in patients with cancer, ... ...

    Abstract During the last two decades, the immunology field has been focused on the study of conventional T-cells, leading to important advances in identification of specific subsets involved in promoting and suppressing immune response in patients with cancer, autoimmune disease or transplanted patients. In these recent years, the research on unconventional subset of CD4
    MeSH term(s) CD4 Antigens/metabolism ; CD8 Antigens/metabolism ; Cell Culture Techniques/methods ; Cytokines/metabolism ; Flow Cytometry/methods ; Leukocytes, Mononuclear/cytology ; Leukocytes, Mononuclear/metabolism ; Phenotype ; Staining and Labeling/methods ; T-Lymphocytes, Cytotoxic/cytology ; T-Lymphocytes, Cytotoxic/metabolism
    Chemical Substances CD4 Antigens ; CD8 Antigens ; Cytokines
    Language English
    Publishing date 2021-05-29
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1507-2_2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book: Cytotoxic T-cells

    Ranieri, Elena

    methods and protocols

    (Methods in molecular biology, ; 1186 ; Springer protocols)

    2014  

    Institution Springer Science+Business Media,
    Author's details edited by Elena Ranieri
    Series title Methods in molecular biology, ; 1186
    Springer protocols
    MeSH term(s) T-Lymphocytes, Cytotoxic ; Systems Biology/methods
    Language English
    Size xii, 301 pages :, illustrations.
    Document type Book
    ISBN 9781493911578 ; 1493911570 ; 9781493911585 ; 1493911589
    Database Catalogue of the US National Library of Medicine (NLM)

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  5. Article ; Online: Preface. Cytotoxic T-cells.

    Ranieri, Elena

    Methods in molecular biology (Clifton, N.J.)

    2014  Volume 1186, Page(s) v

    MeSH term(s) Humans ; Immunologic Techniques ; Systems Biology ; T-Lymphocytes, Cytotoxic
    Language English
    Publishing date 2014
    Publishing country United States
    Document type Editorial ; Introductory Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: CTL ELISPOT Assay and T Cell Detection.

    Ranieri, Elena / Netti, Giuseppe Stefano / Gigante, Margherita

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2325, Page(s) 65–77

    Abstract: Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific T-cells that secrete cytokines and effector ... ...

    Abstract Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows the detection of low-frequency antigen-specific T-cells that secrete cytokines and effector molecules, such as granzyme B and perforin. Cytotoxic T-cell (CTL) studies have taken advantage with this high-throughput technology by providing insights of quantity and immune kinetics. Accuracy, sensitivity, reproducibility, and robustness of Elispot resulted in a wide range of applications in research as well as in diagnostic field. Actually, CTL monitoring by Elispot is a gold standard for the evaluation of antigen-specific T-cell immunity in clinical trials and vaccine candidates where the ability to detect rare antigen-specific T-cells is of relevance for immune diagnostic. The most utilized Elispot assay is the Interferon-gamma (IFN-γ) test, a marker for CD8
    MeSH term(s) CD8-Positive T-Lymphocytes/metabolism ; Cytokines/metabolism ; Enzyme-Linked Immunospot Assay/methods ; Humans ; Interferon-gamma/metabolism ; Perforin/metabolism
    Chemical Substances Cytokines ; Perforin (126465-35-8) ; Interferon-gamma (82115-62-6)
    Language English
    Publishing date 2021-05-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1507-2_5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Kinetics of different blood biomarkers during Polymyxin-B extracorporeal hemoperfusion in abdominal sepsis.

    Cotoia, Antonella / Parisano, Valeria / Mariotti, Paola Sara / Lizzi, Vincenzo / Netti, Giuseppe Stefano / Ranieri, Elena / Forfori, Francesco / Cinnella, Gilda

    Blood purification

    2024  

    Abstract: Introduction Comparison of the marker kinetics procalcitonin, presepsin and endotoxin during extracorporeal hemoperfusion with polymyxin B adsorbing cartridge (PMX-HA) have never been described in abdominal sepsis. We aim to compare the trend of three ... ...

    Abstract Introduction Comparison of the marker kinetics procalcitonin, presepsin and endotoxin during extracorporeal hemoperfusion with polymyxin B adsorbing cartridge (PMX-HA) have never been described in abdominal sepsis. We aim to compare the trend of three biomarkers in septic post-surgical abdominal patients in Intensive care Unit (ICU) treated with PMX-HA and their prognostic value. Methods Ninety abdominal postsurgical patients were enrolled into different groups according to the evidence of postoperative sepsis or not. Non-septic patients admitted in the surgical ward were included in C group (control group). ICU septic shock patients with endotoxin levels <0.6 EAA receiving conventional therapy were addressed in S group and those with endotoxin levels ≥0.6 EAA receiving treatment with PMX-HA, besides conventional therapy, were included in SPB group. Presepsin, procalcitonin, endotoxin and other clinical data were recorded at 24h (T0), 72h (T1) and 7 days (T2) after surgery. Clinical follow-up was performed on day 30. Results SPB group showed reduced levels of the three biomarkers on T2 vs T0 (P<0.001); presepsin, procalcitonin and endotoxin levels decreased respectively of 25%, 11% and 2% on T1 vs T0, and of 40%, 41%, 26% on T2 vs T0. All patients in C group, 73% of patients in SPB group vs 37% of patients in S group survived at follow-up. Moreover, procalcitonin had the highest predictive value for mortality at 30 days, followed by presepsin. Conclusion The present study showed the reliability of presepsin in monitoring PMX-HA treatment in septic shock patients. Procalcitonin showed better predicting power for the mortality risk.
    Language English
    Publishing date 2024-04-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 605548-5
    ISSN 1421-9735 ; 0253-5068
    ISSN (online) 1421-9735
    ISSN 0253-5068
    DOI 10.1159/000538870
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1872: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: Understanding Mechanisms of RKIP Regulation to Improve the Development of New Diagnostic Tools.

    Papale, Massimo / Netti, Giuseppe Stefano / Stallone, Giovanni / Ranieri, Elena

    Cancers

    2022  Volume 14, Issue 20

    Abstract: One of the most dangerous aspects of cancer cell biology is their ability to grow, spread and form metastases in the main vital organs. The identification of dysregulated markers that drive intracellular signalling involved in the malignant ... ...

    Abstract One of the most dangerous aspects of cancer cell biology is their ability to grow, spread and form metastases in the main vital organs. The identification of dysregulated markers that drive intracellular signalling involved in the malignant transformation of neoplastic cells and the understanding of the mechanisms that regulate these processes is undoubtedly a key objective for the development of new and more targeted therapies. RAF-kinase inhibitor protein (RKIP) is an endogenous tumour suppressor protein that affects tumour cell survival, proliferation, and metastasis. RKIP might serve as an early tumour biomarker since it exhibits significantly different expression levels in various cancer histologies and it is often lost during metastatic progression. In this review, we discuss the specific impact of transcriptional, post-transcriptional and post-translational regulation of expression and activation/inhibition of RKIP and focus on those tumours for which experimental data on all these factors are available. In this way, we could select how these processes cooperate with RKIP expression in (1) Lung cancer; (2) Colon cancer, (3) Breast cancer; (4) myeloid neoplasm and Multiple Myeloma, (5) Melanoma and (6) clear cell Renal Cell Carcinoma. Furthermore, since RKIP seems to be a key marker of the development of several tumours and it may be assessed easily in various biological fluids, here we discuss the potential role of RKIP dosing in more accessible biological matrices other than tissues. Moreover, this objective may intercept the still unmet need to identify new and more accurate markers for the early diagnosis and prognosis of many tumours.
    Language English
    Publishing date 2022-10-17
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers14205070
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Protein-Bound Uremic Toxins and Immunity.

    Rocchetti, Maria Teresa / Cosola, Carmela / Ranieri, Elena / Gesualdo, Loreto

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2325, Page(s) 215–227

    Abstract: Protein-bound uremic toxins (PBUTs) are bioactive microbiota metabolites originated exclusively from protein fermentation of the bacterial community resident within the gut microbiota, whose composition and function is profoundly different in the chronic ...

    Abstract Protein-bound uremic toxins (PBUTs) are bioactive microbiota metabolites originated exclusively from protein fermentation of the bacterial community resident within the gut microbiota, whose composition and function is profoundly different in the chronic kidney disease (CKD) population. PBUTs accumulate in the later stages of CKD because they cannot be efficiently removed by conventional hemodialysis due to their high binding affinity for albumin, worsening their toxic effects, especially at the cardiovascular level. The accumulation of uremic toxins, along with oxidative stress products and pro-inflammatory cytokines, characterizes the uremic status of CKD patients which is increasingly associated to a state of immune dysfunction including both immune activation and immunodepression. Furthermore, the links between immune activation and cardiovascular disease (CVD), and between immunodepression and infection diseases, which are the two major complications of CKD, are becoming more and more evident. This review summarizes and discusses the current state of knowledge on the role of the main PBUTs, namely indoxyl sulfate and p-cresyl sulfate, as regulators of immune response in CKD, in order to understand whether a microbiota modulation may be useful in the management of its main complications, CVD, and infections. Summarizing the direct effects of PBUT on immune system we may conclude that PCS seemed to be associated to an immune deficiency status of CKD mainly related to the adaptative immune response, while IS seemed to reflect the activation of both innate and adaptative immune systems likely responsible of the CKD-associated inflammation. However, the exact role of IS and PCS on immunity modulation in physiological and pathological state still needs in-depth investigation, particularly in vivo studies.
    MeSH term(s) Adaptive Immunity ; Cardiovascular Diseases/complications ; Cardiovascular Diseases/metabolism ; Cardiovascular Diseases/urine ; Cresols/metabolism ; Cresols/toxicity ; Gastrointestinal Microbiome/immunology ; Humans ; Immunity, Innate ; Indican/metabolism ; Indican/toxicity ; Inflammation/metabolism ; Renal Insufficiency, Chronic/complications ; Renal Insufficiency, Chronic/immunology ; Renal Insufficiency, Chronic/urine ; Sulfuric Acid Esters/metabolism ; Sulfuric Acid Esters/toxicity ; T-Lymphocytes/immunology ; Toxins, Biological/urine ; Uremia/immunology ; Uremia/metabolism ; Uremia/urine
    Chemical Substances Cresols ; Sulfuric Acid Esters ; Toxins, Biological ; 4-cresol sulfate (56M34ZQY1S) ; Indican (N187WK1Y1J)
    Language English
    Publishing date 2021-05-29
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1507-2_15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Quale ruolo dei microRNA in nefrologia?

    Ranieri, Elena

    Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia

    2010  Volume 27, Issue 6, Page(s) 565

    Title translation Role of microRNA in nephrology.
    MeSH term(s) Biomarkers/metabolism ; Diabetic Nephropathies/metabolism ; Diabetic Nephropathies/pathology ; Humans ; Kidney Diseases/genetics ; Kidney Diseases/metabolism ; Kidney Diseases/pathology ; Kidney Diseases/therapy ; MicroRNAs/antagonists & inhibitors ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Predictive Value of Tests ; Sensitivity and Specificity
    Chemical Substances Biomarkers ; MicroRNAs
    Language Italian
    Publishing date 2010-11
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 1237110-5
    ISSN 0393-5590
    ISSN 0393-5590
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4313: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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