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  1. Book ; Online: Regulation of and by the Plant Cell Wall

    Drakakaki, Georgia / Elizabeth Bartley, Laura / Anderson, Charles T. / Rao, Xiaolan

    2020  

    Keywords Science: general issues ; Botany & plant sciences ; plant cell wall ; transcriptional regulation ; cell wall biosynthesis ; cell wall modification ; vesicle-mediated trafficking ; polysaccharide transport
    Size 1 electronic resource (221 pages)
    Publisher Frontiers Media SA
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021231446
    ISBN 9782889638048 ; 2889638049
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Modeling lignin biosynthesis: a pathway to renewable chemicals.

    Rao, Xiaolan / Barros, Jaime

    Trends in plant science

    2023  Volume 29, Issue 5, Page(s) 546–559

    Abstract: Plant biomass contains lignin that can be converted into high-value-added chemicals, fuels, and materials. The precise genetic manipulation of lignin content and composition in plant cells offers substantial environmental and economic benefits. However, ... ...

    Abstract Plant biomass contains lignin that can be converted into high-value-added chemicals, fuels, and materials. The precise genetic manipulation of lignin content and composition in plant cells offers substantial environmental and economic benefits. However, the intricate regulatory mechanisms governing lignin formation challenge the development of crops with specific lignin profiles. Mathematical models and computational simulations have recently been employed to gain fundamental understanding of the metabolism of lignin and related phenolic compounds. This review article discusses the strategies used for modeling plant metabolic networks, focusing on the application of mathematical modeling for flux network analysis in monolignol biosynthesis. Furthermore, we highlight how current challenges might be overcome to optimize the use of metabolic modeling approaches for developing lignin-engineered plants.
    MeSH term(s) Lignin/biosynthesis ; Lignin/metabolism ; Models, Biological ; Metabolic Networks and Pathways ; Plants/metabolism ; Biomass ; Crops, Agricultural/metabolism
    Chemical Substances Lignin (9005-53-2)
    Language English
    Publishing date 2023-10-04
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1305448-x
    ISSN 1878-4372 ; 1360-1385
    ISSN (online) 1878-4372
    ISSN 1360-1385
    DOI 10.1016/j.tplants.2023.09.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics

    Adolfo, Laci M. / Rao, Xiaolan / Dixon, Richard A.

    BMC Plant Biol. 2022 Dec., v. 22, no. 1 p.10-10

    2022  

    Abstract: BACKGROUND: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of ... ...

    Abstract BACKGROUND: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of daidzein, puerarin. In the US, kudzu is seen as a noxious weed causing ecological and economic damage. However, not all kudzu species make puerarin or are equally invasive. Kudzu remains difficult to identify due to its diverse morphology and inconsistent nomenclature. RESULTS: We have generated sequences for the internal transcribed spacer 2 (ITS2) and maturase K (matK) regions of Pueraria montana lobata, P. montana montana, and P. phaseoloides, and identified two accessions previously used for differential analysis of puerarin biosynthesis as P. lobata and P. phaseoloides. Additionally, we have generated root transcriptomes for the puerarin-producing P. m. lobata and the non-puerarin producing P. phaseoloides. Within the transcriptomes, microsatellites were identified to aid in species identification as well as population diversity. CONCLUSIONS: The barcode sequences generated will aid in fast and efficient identification of the three kudzu species. Additionally, the microsatellites identified from the transcriptomes will aid in genetic analysis. The root transcriptomes also provide a molecular toolkit for comparative gene expression analysis towards elucidation of the biosynthesis of kudzu phytochemicals.
    Keywords DNA barcoding ; Oriental traditional medicine ; Pueraria montana var. lobata ; biosynthesis ; daidzein ; gene expression ; genetic analysis ; internal transcribed spacers ; microsatellite repeats ; noxious weeds ; species identification ; transcriptome ; transcriptomics
    Language English
    Dates of publication 2022-12
    Size p. 10.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2059868-3
    ISSN 1471-2229
    ISSN 1471-2229
    DOI 10.1186/s12870-021-03383-x
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics.

    Adolfo, Laci M / Rao, Xiaolan / Dixon, Richard A

    BMC plant biology

    2022  Volume 22, Issue 1, Page(s) 10

    Abstract: Background: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of ...

    Abstract Background: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of daidzein, puerarin. In the US, kudzu is seen as a noxious weed causing ecological and economic damage. However, not all kudzu species make puerarin or are equally invasive. Kudzu remains difficult to identify due to its diverse morphology and inconsistent nomenclature.
    Results: We have generated sequences for the internal transcribed spacer 2 (ITS2) and maturase K (matK) regions of Pueraria montana lobata, P. montana montana, and P. phaseoloides, and identified two accessions previously used for differential analysis of puerarin biosynthesis as P. lobata and P. phaseoloides. Additionally, we have generated root transcriptomes for the puerarin-producing P. m. lobata and the non-puerarin producing P. phaseoloides. Within the transcriptomes, microsatellites were identified to aid in species identification as well as population diversity.
    Conclusions: The barcode sequences generated will aid in fast and efficient identification of the three kudzu species. Additionally, the microsatellites identified from the transcriptomes will aid in genetic analysis. The root transcriptomes also provide a molecular toolkit for comparative gene expression analysis towards elucidation of the biosynthesis of kudzu phytochemicals.
    MeSH term(s) DNA Barcoding, Taxonomic ; Gene Expression Profiling ; Isoflavones/analysis ; Plant Roots/chemistry ; Plant Weeds/classification ; Plant Weeds/genetics ; Pueraria/classification ; Pueraria/genetics ; Transcriptome
    Chemical Substances Isoflavones ; puerarin (Z9W8997416)
    Language English
    Publishing date 2022-01-03
    Publishing country England
    Document type Journal Article
    ISSN 1471-2229
    ISSN (online) 1471-2229
    DOI 10.1186/s12870-021-03383-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Corrigendum: The Differences between NAD-ME and NADP-ME Subtypes of C

    Rao, Xiaolan / Dixon, Richard A

    Frontiers in plant science

    2019  Volume 10, Page(s) 247

    Abstract: This corrects the article DOI: 10.3389/fpls.2016.01525.]. ...

    Abstract [This corrects the article DOI: 10.3389/fpls.2016.01525.].
    Language English
    Publishing date 2019-03-08
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2711035-7
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2019.00247
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Co-expression networks for plant biology: why and how.

    Rao, Xiaolan / Dixon, Richard A

    Acta biochimica et biophysica Sinica

    2019  Volume 51, Issue 10, Page(s) 981–988

    Abstract: Co-expression network analysis is one of the most powerful approaches for interpretation of large transcriptomic datasets. It enables characterization of modules of co-expressed genes that may share biological functional linkages. Such networks provide ... ...

    Abstract Co-expression network analysis is one of the most powerful approaches for interpretation of large transcriptomic datasets. It enables characterization of modules of co-expressed genes that may share biological functional linkages. Such networks provide an initial way to explore functional associations from gene expression profiling and can be applied to various aspects of plant biology. This review presents the applications of co-expression network analysis in plant biology and addresses optimized strategies from the recent literature for performing co-expression analysis on plant biological systems. Additionally, we describe the combined interpretation of co-expression analysis with other genomic data to enhance the generation of biologically relevant information.
    MeSH term(s) Data Mining/methods ; Gene Expression Profiling/methods ; Gene Expression Regulation, Plant ; Gene Regulatory Networks ; Genomics/methods ; Plant Proteins/genetics ; Plants/genetics ; Transcriptome
    Chemical Substances Plant Proteins
    Language English
    Publishing date 2019-08-22
    Publishing country China
    Document type Journal Article ; Review
    ZDB-ID 2175256-4
    ISSN 1745-7270 ; 0582-9879 ; 1672-9145
    ISSN (online) 1745-7270
    ISSN 0582-9879 ; 1672-9145
    DOI 10.1093/abbs/gmz080
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: FERONIA and wall-associated kinases coordinate defense induced by lignin modification in plant cell walls.

    Liu, Chang / Yu, Hasi / Voxeur, Aline / Rao, Xiaolan / Dixon, Richard A

    Science advances

    2023  Volume 9, Issue 10, Page(s) eadf7714

    Abstract: Altering the content or composition of the cell wall polymer lignin is a favored approach to valorize lignin toward biomaterial and chemical production in the biorefinery. However, modifying lignin or cellulose in transgenic plants can induce expression ... ...

    Abstract Altering the content or composition of the cell wall polymer lignin is a favored approach to valorize lignin toward biomaterial and chemical production in the biorefinery. However, modifying lignin or cellulose in transgenic plants can induce expression of defense responses and negatively affect growth. Through genetic screening for suppressors of defense gene induction in the low lignin
    MeSH term(s) Lignin/metabolism ; Cellulose/metabolism ; Arabidopsis/genetics ; Polysaccharides/metabolism ; Cell Wall/genetics ; Gene Expression Regulation, Plant
    Chemical Substances Lignin (9005-53-2) ; Cellulose (9004-34-6) ; Polysaccharides
    Language English
    Publishing date 2023-03-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.adf7714
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: A smart RBS library and its prediction model for robust and accurate fine-tuning of gene expression in Bacillus species.

    Rao, Xiaolan / Li, Dian / Su, Zhaowei / Nomura, Christopher T / Chen, Shouwen / Wang, Qin

    Metabolic engineering

    2023  Volume 81, Page(s) 1–9

    Abstract: Bacillus species, such as Bacillus subtilis and Bacillus licheniformis, are important industrial bacteria. However, there is a lack of standardized and predictable genetic tools for convenient and reproducible assembly of genetic modules in Bacillus ... ...

    Abstract Bacillus species, such as Bacillus subtilis and Bacillus licheniformis, are important industrial bacteria. However, there is a lack of standardized and predictable genetic tools for convenient and reproducible assembly of genetic modules in Bacillus species to realize their full potential. In this study, we constructed a Ribosome Binding Site (RBS) library in B. licheniformis, which provides incremental regulation of expression levels over a 10
    MeSH term(s) Bacillus/genetics ; Bacillus subtilis/genetics ; Ribosomes/genetics ; Binding Sites ; Gene Expression
    Language English
    Publishing date 2023-11-10
    Publishing country Belgium
    Document type Journal Article
    ZDB-ID 1470383-x
    ISSN 1096-7184 ; 1096-7176
    ISSN (online) 1096-7184
    ISSN 1096-7176
    DOI 10.1016/j.ymben.2023.11.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: A conserved oxalyl-coenzyme A decarboxylase in oxalate catabolism

    Cheng, Ninghui / Paris, Vincent / Rao, Xiaolan / Wang, Xiaoqiang / Nakata, Paul A.

    Plant Signaling & Behavior. 2022 Dec. 31, v. 17, no. 1 p.2062555-

    2022  

    Abstract: The ability to biosynthesize oxalic acid can provide beneficial functions to plants; however, uncontrolled or prolonged exposure to this strong organic acid results in multiple physiological problems. Such problems include a disruption of membrane ... ...

    Abstract The ability to biosynthesize oxalic acid can provide beneficial functions to plants; however, uncontrolled or prolonged exposure to this strong organic acid results in multiple physiological problems. Such problems include a disruption of membrane integrity, mitochondrial function, metal chelation, and free radical formation. Recent work suggests that a CoA-dependent pathway of oxalate catabolism plays a critical role in regulating tissue oxalate concentrations in plants. Although this CoA-dependent pathway of oxalate catabolism is important, large gaps in our knowledge of the enzymes catalyzing each step remain. Evidence that an oxalyl-CoA decarboxylase (OXC) catalyzes the second step in this pathway, accelerating the conversion of oxalyl-CoA to formyl-CoA, has been reported. Induction studies revealed that OXC gene expression was upregulated in response to an exogenous oxalate supply. Phylogenetic analysis indicates that OXCs are conserved across plant species. Evolutionarily the plant OXCs can be separated into dicot and monocot classes. Multiple sequence alignments and molecular modeling suggest that OXCs have similar functionality with three conserved domains, the N-terminal PYR domain, the middle R domain, and the C-terminal PP domain. Further study of this CoA-dependent pathway of oxalate degradation would benefit efforts to develop new strategies to improve the nutrition quality of crops.
    Keywords Liliopsida ; Magnoliopsida ; behavior ; catabolism ; chelation ; free radicals ; gene expression ; mitochondria ; nutrition ; oxalates ; oxalic acid ; phylogeny ; Arabidopsis ; Coenzyme A ; decarboxylase ; oxalate
    Language English
    Dates of publication 2022-1231
    Publishing place Taylor & Francis
    Document type Article ; Online
    ISSN 1559-2324
    DOI 10.1080/15592324.2022.2062555
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Evaluation of pathways to the C‐glycosyl isoflavone puerarin in roots of kudzu (Pueraria montana lobata)

    Adolfo, Laci M. / Burks, David / Rao, Xiaolan / Alvarez‐Hernandez, Anislay / Dixon, Richard A.

    Plant direct. 2022 Sept., v. 6, no. 9

    2022  

    Abstract: Kudzu (Pueraria montana lobata) is used as a traditional medicine in China and Southeast Asia but is a noxious weed in the Southeastern United States. It produces both O‐ and C‐glycosylated isoflavones, with puerarin (C‐glucosyl daidzein) as an important ...

    Abstract Kudzu (Pueraria montana lobata) is used as a traditional medicine in China and Southeast Asia but is a noxious weed in the Southeastern United States. It produces both O‐ and C‐glycosylated isoflavones, with puerarin (C‐glucosyl daidzein) as an important bioactive compound. Currently, the stage of the isoflavone pathway at which the C‐glycosyl unit is added remains unclear, with a recent report of direct C‐glycosylation of daidzein contradicting earlier labeling studies supporting C‐glycosylation at the level of chalcone. We have employed comparative mRNA sequencing of the roots from two Pueraria species, one of which produces puerarin (field collected P. montana lobata) and one of which does not (commercial Pueraria phaseoloides), to identify candidate uridine diphosphate glycosyltransferase (UGT) enzymes involved in puerarin biosynthesis. Expression of recombinant UGTs in Escherichia coli and candidate C‐glycosyltransferases in Medicago truncatula were used to explore substrate specificities, and gene silencing of UGT and key isoflavone biosynthetic genes in kudzu hairy roots employed to test hypotheses concerning the substrate(s) for C‐glycosylation. Our results confirm UGT71T5 as a C‐glycosyltransferase of isoflavone biosynthesis in kudzu. Enzymatic, isotope labeling, and genetic analyses suggest that puerarin arises both from the direct action of UGT71T5 on daidzein and via a second route in which the C‐glycosidic linkage is introduced to the chalcone isoliquiritigenin.
    Keywords Escherichia coli ; Medicago truncatula ; Pueraria montana var. lobata ; Pueraria phaseoloides ; bioactive compounds ; biosynthesis ; chalcone ; daidzein ; genes ; isotopes ; noxious weeds ; traditional medicine ; uridine diphosphate ; China ; South East Asia
    Language English
    Dates of publication 2022-09
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ISSN 2475-4455
    DOI 10.1002/pld3.442
    Database NAL-Catalogue (AGRICOLA)

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