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Article ; Online: Genotype Diversity and Spread of

Onihary, Alain Moïse / Razanajatovo, Iony Manitra / Rabetafika, Lydia / Bastaraud, Alexandra / Heraud, Jean-Michel / Rasolofo, Voahangy

Viruses

2021 Volume 13, Issue 9

Abstract: White Spot Disease (WSD) caused by ... ...

Abstract	White Spot Disease (WSD) caused by the
MeSH term(s)	Animals ; Aquaculture ; Genetic Variation ; Genotype ; Madagascar ; Penaeidae/virology ; White spot syndrome virus 1/classification ; White spot syndrome virus 1/genetics
Language	English
Publishing date	2021-08-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13091713
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Article ; Online: Different PPD-stimulated cytokine responses from patients infected with genetically distinct Mycobacterium tuberculosis complex lineages.

Ranaivomanana, Paulo / Rabodoarivelo, Marie Sylvianne / Ndiaye, Mame Diarra Bousso / Rakotosamimanana, Niaina / Rasolofo, Voahangy

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

2021 Volume 104, Page(s) 725–731

Abstract: Objectives: The genetic diversity of Mycobacterium tuberculosis complex (MTBC) influences the immune response of the host, which may affect the immunodiagnostic tests and biomarker assessment studies used for tuberculosis (TB). This study aimed to ... ...

Abstract	Objectives: The genetic diversity of Mycobacterium tuberculosis complex (MTBC) influences the immune response of the host, which may affect the immunodiagnostic tests and biomarker assessment studies used for tuberculosis (TB). This study aimed to determine whether the mycobacterial-antigen-stimulated cytokine responses vary with the genotype of the MTBC infecting the patient. Methods: Eighty-one patients with confirmed active pulmonary TB were recruited, and MTBC clinical strains were isolated from their sputum for bacterial lineage single-nucleotide polymorphism typing. Whole blood was drawn from the patients to measure the purified protein derivative (PPD)-stimulated cytokine responses (GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, TNF-α, IFN-α, IL-12, eotaxin, IL-13, IL-15, IL-17, MIP1-α, MIP1-β, MCP1, IL1RA, IP10, IL2R, MIG) with the Luminex multiplex immunoassay. Results: Of the 24 cytokines studied, three were produced differentially in whole blood dependent on the infecting lineage of MTBC. Decreased production of IL-17 was observed in patients infected with modern lineages compared with patients infected with ancestral lineages (P < 0.01), and production of IFN-γ and IL-2 was significantly decreased in patients infected with lineage 4 strains compared with patients infected with lineage 3 strains (P < 0.05). Conclusion: MTBC strains belonging to lineage 4 induced a decreased whole-blood PPD-stimulated pro-inflammatory cytokine response.
MeSH term(s)	Adult ; Biomarkers/blood ; Cytokines/blood ; Cytokines/immunology ; Diagnostic Tests, Routine ; Female ; Humans ; Immunoassay ; Interleukins/blood ; Interleukins/immunology ; Male ; Mycobacterium tuberculosis/classification ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/immunology ; Tuberculin/immunology ; Tuberculosis/blood ; Tuberculosis/diagnosis ; Tuberculosis/immunology ; Tuberculosis/microbiology ; Tumor Necrosis Factor-alpha/blood ; Tumor Necrosis Factor-alpha/immunology ; Young Adult
Chemical Substances	Biomarkers ; Cytokines ; Interleukins ; Tuberculin ; Tumor Necrosis Factor-alpha
Language	English
Publishing date	2021-02-05
Publishing country	Canada
Document type	Journal Article
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2021.01.073
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Article: Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

Onihary, Alain Moïse / Razanajatovo, Iony Manitra / Rabetafika, Lydia / Bastaraud, Alexandra / Heraud, Jean-Michel / Rasolofo, Voahangy

Viruses. 2021 Aug. 28, v. 13, no. 9

2021

Abstract: White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known ... ...

Abstract	White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its genetic diversity. Our study aimed at describing the molecular diversity and the spread of WSSV in the populations of Madagascan crustaceans. Farmed and wild shrimps were collected from various locations in Madagascar from 2012 to 2016 and were tested for WSSV. Amplicons from positive specimens targeting five molecular markers (ORF75, ORF94, ORF125, VR14/15 and VR23/24) were sequenced for genotyping characterizations. Four genotypes were found in Madagascar. The type-I genotype was observed in the south-west of Madagascar in April 2012, causing a disastrous epidemic, then spread to the North-West coast. Type-II strains were detected in October 2012 causing an outbreak in another Penaeus monodon farm. In 2014 and 2015, types II and III were observed in shrimp farms. Finally, in 2016, types II and IV were found in wild species including Fenneropenaeus indicus, Metapenaeus monoceros, Marsupenaeus japonicus and Macrobrachium rosenbergii. Considering the economic importance of the shrimp industry for Madagascar, our study highlights the need to maintain WSSV surveillance to quickly take appropriate countermeasures in case of outbreak and to sustain this industry.
Keywords	Fenneropenaeus indicus ; Macrobrachium rosenbergii ; Marsupenaeus japonicus ; Metapenaeus monoceros ; Penaeus monodon ; White spot syndrome virus ; coasts ; farms ; genetic variation ; genotype ; genotyping ; monitoring ; shrimp ; shrimp culture ; shrimp fisheries ; viruses ; Madagascar
Language	English
Dates of publication	2021-0828
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v13091713
Database	NAL-Catalogue (AGRICOLA)

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Article: Using a multiplex serological assay to estimate time since SARS-CoV-2 infection and past clinical presentation in malagasy patients.

Ndiaye, Mame Diarra Bousso / Rasoloharimanana, Lova Tsikiniaina / Razafimahatratra, Solohery Lalaina / Ratovoson, Rila / Rasolofo, Voahangy / Ranaivomanana, Paulo / Raskine, Laurent / Hoffmann, Jonathan / Randremanana, Rindra / Rakotosamimanana, Niaina / Schoenhals, Matthieu

Heliyon

2023 Volume 9, Issue 6, Page(s) e17264

Abstract: Background: The world is facing a 2019 coronavirus (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this context, efficient serological assays are needed to accurately describe the humoral responses against ... ...

Abstract	Background: The world is facing a 2019 coronavirus (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this context, efficient serological assays are needed to accurately describe the humoral responses against the virus. These tools could potentially provide temporal and clinical characteristics and are thus paramount in developing-countries lacking sufficient ongoing COVID-19 epidemic descriptions. Methods: We developed and validated a Luminex xMAP® multiplex serological assay targeting specific IgM and IgG antibodies against the SARS-CoV-2 Spike subunit 1 (S1), Spike subunit 2 (S2), Spike Receptor Binding Domain (RBD) and the Nucleocapsid protein (N). Blood samples collected periodically for 12 months from 43 patients diagnosed with COVID-19 in Madagascar were tested for these antibodies. A random forest algorithm was used to build a predictive model of time since infection and symptom presentation. Findings: The performance of the multiplex serological assay was evaluated for the detection of SARS-CoV-2 Interpretation: This study demonstrates that the statistical model predicts time since infection and previous symptom presentation using IgM and IgG response to SARS-CoV2. This tool may be useful for global surveillance, discriminating recent- and past- SARS-CoV-2 infection, and assessing disease severity. Fundings: This study was funded by the French Ministry for Europe and Foreign Affairs through the REPAIR COVID-19-Africa project coordinated by the Pasteur International Network association. WANTAI reagents were provided by WHO AFRO as part of a Sero-epidemiological "Unity" Study Grant/Award Number: 2020/1,019,828-0 P·O 202546047 and Initiative 5% grant n°AP-5PC-2018-03-RO.
Language	English
Publishing date	2023-06-13
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e17264
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Article ; Online: Plasma host protein signatures correlating with Mycobacterium tuberculosis activity prior to and during antituberculosis treatment.

Ndiaye, Mame Diarra Bousso / Ranaivomanana, Paulo / Rasoloharimanana, Lova Tsikiniaina / Rasolofo, Voahangy / Ratovoson, Rila / Herindrainy, Perlinot / Rakotonirina, Julio / Schoenhals, Matthieu / Hoffmann, Jonathan / Rakotosamimanana, Niaina

Scientific reports

2022 Volume 12, Issue 1, Page(s) 20640

Abstract: There is a need for rapid non-sputum-based tests to identify and treat patients infected with Mycobacterium tuberculosis (Mtb). The overall objective of this study was to measure and compare the expression of a selected panel of human plasma proteins in ... ...

Abstract	There is a need for rapid non-sputum-based tests to identify and treat patients infected with Mycobacterium tuberculosis (Mtb). The overall objective of this study was to measure and compare the expression of a selected panel of human plasma proteins in patients with active pulmonary tuberculosis (ATB) throughout anti-TB treatment (from baseline to the end of treatment), in Mtb-infected individuals (TBI) and healthy donors (HD) to identify a putative host-protein signature useful for both TB diagnosis and treatment monitoring. A panel of seven human host proteins CLEC3B, SELL, IGFBP3, IP10, CD14, ECM1 and C1Q were measured in the plasma isolated from an HIV-negative prospective cohort of 37 ATB, 24 TBI and 23 HD. The protein signatures were assessed using a Luminex xMAP® to quantify the plasmatic levels in unstimulated blood of the different clinical group as well as the protein levels at baseline and at three timepoints during the 6-months ATB treatment, to compare the plasma protein levels between culture slow and fast converters that may contribute to monitor the TB treatment outcome. Protein signatures were defined using the CombiROC algorithm and multivariate models. The studied plasma host proteins showed different levels between the clinical groups and during the TB treatment. Six of the plasma proteins (CLEC3B, SELL, IGFBP3, IP10, CD14 and C1Q) showed significant differences in normalised median fluorescence intensities when comparing ATB vs HD or TBI groups while ECM1 revealed a significant difference between fast and slow sputum culture converters after 2 months following treatment (p = 0.006). The expression of a four-host protein markers (CLEC3B-ECM1-IP10-SELL) was significantly different between ATB from HD or TBI groups (respectively, p < 0.05). The expression of the same signature was significantly different between the slow vs the fast sputum culture converters after 2 months of treatment (p < 0.05). The results suggest a promising 4 host-plasma marker signature that would be associated with both TB diagnostic and treatment monitoring.
MeSH term(s)	Humans ; Mycobacterium tuberculosis ; Chemokine CXCL10 ; Complement C1q ; Prospective Studies ; Antitubercular Agents/therapeutic use ; Tuberculosis, Lymph Node ; Blood Proteins ; Extracellular Matrix Proteins
Chemical Substances	Chemokine CXCL10 ; Complement C1q (80295-33-6) ; Antitubercular Agents ; Blood Proteins ; ECM1 protein, human ; Extracellular Matrix Proteins
Language	English
Publishing date	2022-11-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-25236-9
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Article ; Online: GeneXpert for the diagnosis of COVID-19 in LMICs.

Rakotosamimanana, Niaina / Randrianirina, Frédérique / Randremanana, Rindra / Raherison, Mamy S / Rasolofo, Voahangy / Solofomalala, Gaetan D / Spiegel, André / Heraud, Jean-Michel

The Lancet. Global health

2020 Volume 8, Issue 12, Page(s) e1457–e1458

MeSH term(s)	COVID-19/diagnosis ; COVID-19/virology ; Developing Countries ; Health Resources ; Humans ; Madagascar ; Mycobacterium tuberculosis/genetics ; Pandemics ; Polymerase Chain Reaction/methods ; Rifampin ; SARS-CoV-2/genetics ; Sensitivity and Specificity ; Tuberculosis/diagnosis ; Tuberculosis/microbiology
Chemical Substances	Rifampin (VJT6J7R4TR)
Keywords	covid19
Language	English
Publishing date	2020-10-19
Publishing country	England
Document type	Letter
ZDB-ID	2723488-5
ISSN	2214-109X ; 2214-109X
ISSN (online)	2214-109X
ISSN	2214-109X
DOI	10.1016/S2214-109X(20)30428-9
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Article ; Online: Multiple Introductions of Yersinia pestis during Urban Pneumonic Plague Epidemic, Madagascar, 2017.

Andrianaivoarimanana, Voahangy / Savin, Cyril / Birdsell, Dawn N / Vogler, Amy J / Le Guern, Anne-Sophie / Rahajandraibe, Soloandry / Brémont, Sylvie / Rahelinirina, Soanandrasana / Sahl, Jason W / Ramasindrazana, Beza / Rakotonanahary, Rado Jean Luc / Rakotomanana, Fanjasoa / Randremanana, Rindra / Maheriniaina, Viviane / Razafimbia, Vaoary / Kwasiborski, Aurelia / Balière, Charlotte / Ratsitorahina, Maherisoa / Baril, Laurence /

Keim, Paul / Caro, Valérie / Rasolofo, Voahangy / Spiegel, André / Pizarro-Cerda, Javier / Wagner, David M / Rajerison, Minoarisoa

Emerging infectious diseases

2024 Volume 30, Issue 2, Page(s) 289–298

Abstract: Pneumonic plague (PP) is characterized by high infection rate, person-to-person transmission, and rapid progression to severe disease. In 2017, a PP epidemic occurred in 2 Madagascar urban areas, Antananarivo and Toamasina. We used epidemiologic data and ...

Abstract	Pneumonic plague (PP) is characterized by high infection rate, person-to-person transmission, and rapid progression to severe disease. In 2017, a PP epidemic occurred in 2 Madagascar urban areas, Antananarivo and Toamasina. We used epidemiologic data and Yersinia pestis genomic characterization to determine the sources of this epidemic. Human plague emerged independently from environmental reservoirs in rural endemic foci >20 times during August-November 2017. Confirmed cases from 5 emergences, including 4 PP cases, were documented in urban areas. Epidemiologic and genetic analyses of cases associated with the first emergence event to reach urban areas confirmed that transmission started in August; spread to Antananarivo, Toamasina, and other locations; and persisted in Antananarivo until at least mid-November. Two other Y. pestis lineages may have caused persistent PP transmission chains in Antananarivo. Multiple Y. pestis lineages were independently introduced to urban areas from several rural foci via travel of infected persons during the epidemic.
MeSH term(s)	Humans ; Plague/epidemiology ; Yersinia pestis/genetics ; Madagascar/epidemiology ; Epidemics ; Genomics
Language	English
Publishing date	2024-01-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid3002.230759
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Article ; Online: SARS-CoV-2 antibody seroprevalence follow-up in Malagasy blood donors during the 2020 COVID-19 Epidemic.

Schoenhals, Matthieu / Rabenindrina, Niry / Rakotondramanga, Jean Marius / Dussart, Philippe / Randremanana, Rindra / Heraud, Jean-Michel / Andriamandimby, Soa Fy / Sahondranirina, Paquerette Hanitriniala / Vololoniaina, Manuela Christophère Andriamahatana / Randriatsarafara, Fidiniana Mamy / Rasolofo, Voahangy / Randriamanantany, Zely Arivelo / Spiegel, André

EBioMedicine

2021 Volume 68, Page(s) 103419

Abstract: Background: The incidence of the 2020 COVID-19 epidemic in Africa seems to be different from that of the rest of the world, however its true extent is probably underestimated. Conducting population based sero-surveys during the epidemic has moreover ... ...

Abstract	Background: The incidence of the 2020 COVID-19 epidemic in Africa seems to be different from that of the rest of the world, however its true extent is probably underestimated. Conducting population based sero-surveys during the epidemic has moreover been extremely challenging, driving our group and others to study blood donor samples. Methods: We collected regional epidemiological COVID-19 surveillance data, and simultaneously monitored anti-SARS-CoV-2 antibody seroprevalences monthly throughout the epidemic in 5 major Region-associated Blood Transfusion Centres of Madagascar over a period of 9 months. Findings: Soon after attaining the first epidemic peaks between May and August 2020, both crude and population-weighted test-performance-adjusted seroprevalences of anti-SARS-CoV-2 antibodies was in Malagasy blood donors rapidly increased up to over 40% positivity. Interpretation: These findings suggest a high cumulative incidence of infection and seroconversion, which may have contributed to the observed deceleration of infection rates, but was not sufficient to prevent the second epidemic wave that struck Madagascar in Spring 2021. Funding: This project was funded by the United States Agency for International Development.
MeSH term(s)	Antibodies, Viral/blood ; Blood Donors ; COVID-19/epidemiology ; COVID-19/immunology ; Epidemics ; Female ; Humans ; Incidence ; Madagascar/epidemiology ; Male ; Population Surveillance ; SARS-CoV-2/immunology ; Seroconversion ; Seroepidemiologic Studies
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	2021-06-04
Publishing country	Netherlands
Document type	Journal Article ; Multicenter Study
ZDB-ID	2851331-9
ISSN	2352-3964
ISSN (online)	2352-3964
DOI	10.1016/j.ebiom.2021.103419
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Article ; Online: Performance and impact of GeneXpert MTB/RIF® and Loopamp MTBC Detection Kit® assays on tuberculosis case detection in Madagascar.

Rakotosamimanana, Niaina / Lapierre, Simon Grandjean / Raharimanga, Vaomalala / Raherison, Mamy Serge / Knoblauch, Astrid M / Raherinandrasana, Antso Hasina / Rakotoson, Andrianantenaina / Rakotonirina, Julio / Rasolofo, Voahangy

BMC infectious diseases

2019 Volume 19, Issue 1, Page(s) 542

Abstract: Background: Tuberculosis rapid molecular assays, including GeneXpert MTB/RIF® and Loopamp MTBC Detection Kit®, are highly sensitive and specific. Such performance does not automatically translate in improved disease control and highly depends on their ... ...

Abstract	Background: Tuberculosis rapid molecular assays, including GeneXpert MTB/RIF® and Loopamp MTBC Detection Kit®, are highly sensitive and specific. Such performance does not automatically translate in improved disease control and highly depends on their use, local epidemiology and the diagnostic algorithms they're implemented within. We evaluate the performance of both assays and assess their impact on additional cases notification when implemented within WHO recommended tuberculosis diagnostic algorithms in Madagascar. Methods: Five hundred forty eight presumptive pulmonary tuberculosis patients were prospectively recruited between November 2013 and December 2014 in Antananarivo, Madagascar, a high TB incidence sub-Saharan African urban setting. Both molecular assays were evaluated as first line or add-on testing following negative smear microscopy. Based on locally defined assay performance characteristics we measure the impact of both assays and WHO-recommended diagnostic algorithms on additional tuberculosis case notifications. Results: High sensitivity and specificity was confirmed for both GeneXpert MTB/RIF® (86.6% (95% CI 81.1-90.7%) and 97.4% (95% CI 94.9-98.8%)) and Loopamp MTBC Detection Kit® (84.6% (95% CI 78.9-89.0%) and 98.4% (95% CI 96.2-99.4%)). Implementation of GeneXpert MTB/RIF® and Loopamp MTBC Detection Kit® increased tuberculosis diagnostic algorithms sensitivity from 73.6% (95% CI 67.1-79.3%) up to 88.1% (95% CI 82.8-91.9%). This increase was highest when molecular assays were used as add-on testing following negative smear microscopy. As add-on testing, GeneXpert MTB/RIF® and Loopamp MTBC Detection Kit® respectively improved case detection by 23.8 and 21.2% (p < 0.05). Conclusion: Including GeneXpert MTB/RIF® or Loopamp MTBC Detection Kit® molecular assays for TB detection on sputum samples from presumptive TB cases can significantly increase case notification in TB diagnostic centers. The TB case detection rate is further increased when those tests are use as second-line follow-on testing following negative smear microscopy results. A country wide scale-up and digital integration of molecular-based TB diagnosis assays shows promises for TB control in Madagascar.
MeSH term(s)	Adult ; DNA, Bacterial/isolation & purification ; DNA, Bacterial/metabolism ; Female ; Humans ; Madagascar ; Male ; Middle Aged ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Nucleic Acid Amplification Techniques/methods ; Reagent Kits, Diagnostic ; Sensitivity and Specificity ; Sputum/microbiology ; Tuberculosis, Pulmonary/diagnosis ; Tuberculosis, Pulmonary/microbiology
Chemical Substances	DNA, Bacterial ; Reagent Kits, Diagnostic
Language	English
Publishing date	2019-06-20
Publishing country	England
Document type	Journal Article
ISSN	1471-2334
ISSN (online)	1471-2334
DOI	10.1186/s12879-019-4198-6
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Article ; Online: Optimizing of a protein extraction method for Mycobacterium tuberculosis proteome analysis using mass spectrometry.

Rabodoarivelo, Marie Sylvianne / Aerts, Maarten / Vandamme, Peter / Palomino, Juan Carlos / Rasolofo, Voahangy / Martin, Anandi

Journal of microbiological methods

2016 Volume 131, Page(s) 144–147

Abstract: A critical step in proteomic analyses comprises the implementation of a reliable cell lysis method with high yields of qualitative proteins. In Mycobacteria, the protein extraction step is often hampered by the thick waxy cell wall which is rich in ... ...

Abstract	A critical step in proteomic analyses comprises the implementation of a reliable cell lysis method with high yields of qualitative proteins. In Mycobacteria, the protein extraction step is often hampered by the thick waxy cell wall which is rich in mycolic acids. Harsh disruption techniques to release proteins from the cells are thus required. Here, we demonstrate an optimized protein extraction procedure for Mycobacterium tuberculosis (Mbt) that results in protein extracts that are useful for all currently used proteomics platforms, including gel and LC-MS based strategies. We compared the effectiveness of using both thiourea and urea and/or SDS and DTT in the solubilization buffer, in combination or not with sonication and/or bead beating. After some preliminary optimization steps on fast-growing Mbt-like organisms, namely Mycobacterium smegmatis and Mycobacterium fortuitum, the final protein extraction protocol was tested on M. tuberculosis. Based on the concentrations of the proteins recovered from each of the tested methods and on the quality of the extracted proteins as evaluated by SDS PAGE, we propose a lysis buffer that contains both thiourea and urea, in combination with two mechanical cell disruption methods: sonication and bead beating. The optimized protocol results in protein extracts that are useful in M. tuberculosis proteomics studies based on any proteomics strategy or platform.
Language	English
Publishing date	2016-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2016.10.021
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