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  1. Article ; Online: Percutaneous closure of a post-surgical ascending aortic pseudoaneurysm with an amplatzer septal occluder device and steerable guiding sheath.

    McClure, Graham R / Ratnayake, Geemitha / Chan, Albert / Gagnon, Joel

    Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions

    2021  Volume 98, Issue 7, Page(s) E985–E989

    Abstract: Thoracic pseudoaneurysm in the ascending aorta is an uncommon condition associated with significant risk of morbidity and mortality. Treatment is recommended in all cases regardless of symptoms as the mortality rate if left untreated has been documented ... ...

    Abstract Thoracic pseudoaneurysm in the ascending aorta is an uncommon condition associated with significant risk of morbidity and mortality. Treatment is recommended in all cases regardless of symptoms as the mortality rate if left untreated has been documented to be as high as 61%. The current standard of care for managing these lesions is open surgical repair. However, this is associated with significant morbidity. In-hospital mortality reported for patients undergoing surgical repair of an ascending aortic pseudoaneurysm ranges from 6.7% to 41%. When anatomically suitable, a less invasive approach using amplatzer vascular plug or septal occluder is an attractive approach. We present a case report of repair of a post-surgical ascending aortic false aneurysm using an amplatzer septal occluder with an Oscor ™ steerable guiding sheath; a novel approach to increase platform stability when engaging an aneurysm neck. Endovascular occluder deployment for closure of aortic false aneurysms remains a relatively novel technique. It is limited by the requirement to develop a stable endovascular platform to deliver the device and avoid system prolapse, particularly when accessing challenging lesions on the inner aortic curvature. We present the first case to utilize a steerable guiding sheath system to improve system stability and facilitate successful device delivery. Given the significant morbidity associated with open repair of these lesions we hope this will further expand the range of lesions viewed as appropriate for endovascular repair.
    MeSH term(s) Aneurysm, False/diagnostic imaging ; Aneurysm, False/etiology ; Aneurysm, False/surgery ; Aorta ; Aortic Aneurysm/diagnostic imaging ; Aortic Aneurysm/surgery ; Humans ; Septal Occluder Device ; Treatment Outcome
    Language English
    Publishing date 2021-09-29
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 1459995-8
    ISSN 1522-726X ; 1522-1946
    ISSN (online) 1522-726X
    ISSN 1522-1946
    DOI 10.1002/ccd.29951
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Adverse effects of naphthenic acids on reproductive health: A focus on placental trophoblast cells.

    Raez-Villanueva, Sergio / Jamshed, Laiba / Ratnayake, Geemitha / Cheng, Lisa / Thomas, Philippe J / Holloway, Alison C

    Reproductive toxicology (Elmsford, N.Y.)

    2019  Volume 90, Page(s) 126–133

    Abstract: There is considerable concern that naphthenic acids (NA) related to oil extraction can negatively impact reproduction in mammals yet the mechanisms are unknown. Since placental dysfunction is central to many adverse pregnancy outcomes, the goal of this ... ...

    Abstract There is considerable concern that naphthenic acids (NA) related to oil extraction can negatively impact reproduction in mammals yet the mechanisms are unknown. Since placental dysfunction is central to many adverse pregnancy outcomes, the goal of this study was to determine the effects of NA exposure on placental trophoblast cell function. Htr-8/SVneo cells were exposed to a commercial technical NA mixture (Sigma-Aldrich) for 24 h to assess steroid production, markers of inflammation and oxidative stress. NA treatment significantly altered steroid production; progesterone was decreased at all doses tested, whereas there was a significant increase in testosterone production (125 mg/L only). There were no effects on estradiol production. In addition, NA treatment resulted in increased markers of inflammation (interleukin 1β and prostaglandin E
    MeSH term(s) Carboxylic Acids/toxicity ; Cell Line ; Dinoprostone/metabolism ; Female ; Humans ; Interleukin-1beta/genetics ; Oil and Gas Fields ; Oxidative Stress/drug effects ; Pregnancy ; Progesterone/metabolism ; Reproductive Health ; Testosterone/metabolism ; Trophoblasts/drug effects ; Trophoblasts/metabolism ; Water Pollutants, Chemical/toxicity
    Chemical Substances Carboxylic Acids ; IL1B protein, human ; Interleukin-1beta ; Water Pollutants, Chemical ; Testosterone (3XMK78S47O) ; Progesterone (4G7DS2Q64Y) ; Dinoprostone (K7Q1JQR04M) ; naphthenic acid (YNM5U6B7A4)
    Language English
    Publishing date 2019-09-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639342-1
    ISSN 1873-1708 ; 0890-6238
    ISSN (online) 1873-1708
    ISSN 0890-6238
    DOI 10.1016/j.reprotox.2019.09.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Determination of 4(5)-methylimidazole in carbonated beverages by isotope-dilution liquid chromatography-tandem mass spectrometry.

    Ratnayake, Geemitha / Halldorson, Thor / Bestvater, Lianna / Tomy, Gregg T

    Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment

    2015  Volume 32, Issue 7, Page(s) 1075–1082

    Abstract: The purpose of this study was to develop a method to quantify 4(5)-methylimidazole (4-MEI), a suspected carcinogen, in carbonated beverages by simple sample dilution and isotope-dilution reverse-phase LC-MS/MS. Isotope dilution using hexa-deuterated ... ...

    Abstract The purpose of this study was to develop a method to quantify 4(5)-methylimidazole (4-MEI), a suspected carcinogen, in carbonated beverages by simple sample dilution and isotope-dilution reverse-phase LC-MS/MS. Isotope dilution using hexa-deuterated methylimidazole (d6-4-MEI) was used to quantify native 4-MEI and to assess matrix effects quantitatively. The accuracy of the method was assessed by intentionally fortifying a negative control sample at three doses: low, medium and high (replicates of n = 5 each) with a known amount of 4-MEI. The respective absolute error in each case was 18.7 ± 0.7%, 14.6 ± 2.8% and 21.1 ± 9.7%. Within-day (intra-) and day-to-day (inter-) repeatability, determined as the relative standard deviation by fortifying a negative control sample (n = 5), were 9.5% and 15.4%, respectively. Average ion suppression of d6-4-MEI in beer was 63.9 ± 3.2%, while no suppression or enhancement was seen in non-alcoholic samples. The instrument and method limit of detection were calculated as 0.6 and 5.8 ng ml(-1), respectively. 4(5)-Methylimidazole was quantified in a variety of store-bought consumer beverages and it was found that in many of the samples tested consuming a single can of beer would result in intake levels of 4-MEI that exceed the no significant risk guideline of 29 µg day(-1). Conversely, 4-MEI in the samples was orders of magnitude smaller than the European Food Safety Authority acceptable daily intake threshold value of 100 mg kg(-1) bw day(-1).
    MeSH term(s) Carbonated Beverages/analysis ; Chromatography, Liquid/methods ; Food Analysis/methods ; Imidazoles/chemistry ; Molecular Structure ; Reproducibility of Results ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry/methods
    Chemical Substances Imidazoles ; 4-methylimidazole (Q64GF9FV4I)
    Language English
    Publishing date 2015
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2462951-0
    ISSN 1944-0057 ; 1944-0049
    ISSN (online) 1944-0057
    ISSN 1944-0049
    DOI 10.1080/19440049.2015.1045557
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Determination of 4(5)-methylimidazole in carbonated beverages by isotope-dilution liquid chromatography-tandem mass spectrometry

    Ratnayake, Geemitha / Halldorson, Thor / Bestvater, Lianna / Tomy, Gregg T

    Food additives & contaminants. 2015 July 3, v. 32, no. 7

    2015  

    Abstract: The purpose of this study was to develop a method to quantify 4(5)-methylimidazole (4-MEI), a suspected carcinogen, in carbonated beverages by simple sample dilution and isotope-dilution reverse-phase LC-MS/MS. Isotope dilution using hexa-deuterated ... ...

    Abstract The purpose of this study was to develop a method to quantify 4(5)-methylimidazole (4-MEI), a suspected carcinogen, in carbonated beverages by simple sample dilution and isotope-dilution reverse-phase LC-MS/MS. Isotope dilution using hexa-deuterated methylimidazole (d ₆-4-MEI) was used to quantify native 4-MEI and to assess matrix effects quantitatively. The accuracy of the method was assessed by intentionally fortifying a negative control sample at three doses: low, medium and high (replicates of n = 5 each) with a known amount of 4-MEI. The respective absolute error in each case was 18.7 ± 0.7%, 14.6 ± 2.8% and 21.1 ± 9.7%. Within-day (intra-) and day-to-day (inter-) repeatability, determined as the relative standard deviation by fortifying a negative control sample (n = 5), were 9.5% and 15.4%, respectively. Average ion suppression of d ₆-4-MEI in beer was 63.9 ± 3.2%, while no suppression or enhancement was seen in non-alcoholic samples. The instrument and method limit of detection were calculated as 0.6 and 5.8 ng ml –¹, respectively. 4(5)-Methylimidazole was quantified in a variety of store-bought consumer beverages and it was found that in many of the samples tested consuming a single can of beer would result in intake levels of 4-MEI that exceed the no significant risk guideline of 29 µg day –¹. Conversely, 4-MEI in the samples was orders of magnitude smaller than the European Food Safety Authority acceptable daily intake threshold value of 100 mg kg –¹ bw day –¹.
    Keywords acceptable daily intake ; beers ; carbonated beverages ; carcinogens ; detection limit ; food safety ; guidelines ; isotope dilution technique ; liquid chromatography ; risk ; tandem mass spectrometry
    Language English
    Dates of publication 2015-0703
    Size p. 1075-1082.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2462951-0
    ISSN 1944-0057 ; 1944-0049
    ISSN (online) 1944-0057
    ISSN 1944-0049
    DOI 10.1080/19440049.2015.1045557
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Impacts of Unregulated Novel Brominated Flame Retardants on Human Liver Thyroid Deiodination and Sulfotransferation

    Smythe, Tristan A / Butt Craig M / Konstantinov Alex / Pleskach Kerri / Ratnayake Geemitha / Riddell Nicole / Song Chae Yoon / Stapleton Heather M / Tomy Gregg T

    Environmental Science & Technology. 2017 June 20, v. 51, no. 12

    2017  

    Abstract: The inhibitory effects of five novel brominated flame retardants, 1,2-bis(2,4,5-tribromophenoxy)ethane (BTBPE), decabromodiphenylethane (DBDPE), 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB), bis(2-ethylhexyl)tetrabromophthalate (BEH-TEBP), and β- ... ...

    Abstract The inhibitory effects of five novel brominated flame retardants, 1,2-bis(2,4,5-tribromophenoxy)ethane (BTBPE), decabromodiphenylethane (DBDPE), 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB), bis(2-ethylhexyl)tetrabromophthalate (BEH-TEBP), and β-tetrabromoethylcyclohexane (β-TBECH), on thyroid hormone deiodinase (DIO) and sulfotransferase (SULT) activity were investigated using human in vitro liver microsomal and cytosolic bioassays. Enzymatic activity was measured by incubating active human liver subcellular fractions with thyroid hormones (T4 and rT3 separately) and measuring changes in thyroid hormone (T4, T3, rT3, and 3,3′-T2) concentrations. Only DBDPE showed inhibition of both outer and inner ring deiodination (O and IRD) of T3 and 3,3′-T2 formation from T4, respectively, with an estimated IC₅₀ of 160 nM; no statistically significant inhibition of SULT activity was observed. ORD inhibition of 3,3′-T2 formation from rT3 was also observed (IC₅₀ ∼ 100 nM). The kinetics of T4 O and IRD were also investigated, although a definitive mechanism could not be identified as the Michaelis–Menten parameters and maximal rate constants were not significantly different. Concentrations tested were intentionally above expected environmental levels, and this study suggests that these NBFRs are not potent human liver DIO and SULT inhibitors. To our knowledge, DBDPE is the first example of a nonhydroxylated contaminant inhibiting DIO activity, and further study of the mechanism of action is warranted.
    Keywords bioassays ; enzyme activity ; ethane ; flame retardants ; humans ; inhibitory concentration 50 ; liver ; mechanism of action ; subcellular fractions ; thyroid hormones
    Language English
    Dates of publication 2017-0620
    Size p. 7245-7253.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1520-5851
    DOI 10.1021%2Facs.est.7b01143
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Impacts of Unregulated Novel Brominated Flame Retardants on Human Liver Thyroid Deiodination and Sulfotransferation.

    Smythe, Tristan A / Butt, Craig M / Stapleton, Heather M / Pleskach, Kerri / Ratnayake, Geemitha / Song, Chae Yoon / Riddell, Nicole / Konstantinov, Alex / Tomy, Gregg T

    Environmental science & technology

    2017  Volume 51, Issue 12, Page(s) 7245–7253

    Abstract: The inhibitory effects of five novel brominated flame retardants, 1,2-bis(2,4,5-tribromophenoxy)ethane (BTBPE), decabromodiphenylethane (DBDPE), 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB), bis(2-ethylhexyl)tetrabromophthalate (BEH-TEBP), and β- ... ...

    Abstract The inhibitory effects of five novel brominated flame retardants, 1,2-bis(2,4,5-tribromophenoxy)ethane (BTBPE), decabromodiphenylethane (DBDPE), 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB), bis(2-ethylhexyl)tetrabromophthalate (BEH-TEBP), and β-tetrabromoethylcyclohexane (β-TBECH), on thyroid hormone deiodinase (DIO) and sulfotransferase (SULT) activity were investigated using human in vitro liver microsomal and cytosolic bioassays. Enzymatic activity was measured by incubating active human liver subcellular fractions with thyroid hormones (T4 and rT3 separately) and measuring changes in thyroid hormone (T4, T3, rT3, and 3,3'-T2) concentrations. Only DBDPE showed inhibition of both outer and inner ring deiodination (O and IRD) of T3 and 3,3'-T2 formation from T4, respectively, with an estimated IC
    Language English
    Publishing date 2017-06-20
    Publishing country United States
    Document type Journal Article
    ISSN 1520-5851
    ISSN (online) 1520-5851
    DOI 10.1021/acs.est.7b01143
    Database MEDical Literature Analysis and Retrieval System OnLINE

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