LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article ; Online: Venomics and antivenomics of Indian spectacled cobra (Naja naja) from the Western Ghats.

    Vanuopadath, Muralidharan / Raveendran, Dileepkumar / Nair, Bipin Gopalakrishnan / Nair, Sudarslal Sadasivan

    Acta tropica

    2022  Volume 228, Page(s) 106324

    Abstract: Venom proteome profiling of Naja naja from the Western Ghats region in Kerala was achieved through SDS-PAGE and RP-HPLC followed by Q-TOF LC-MS/MS analysis, incorporating PEAKS and Novor assisted de novo sequencing methodologies. A total of 115 proteins ... ...

    Abstract Venom proteome profiling of Naja naja from the Western Ghats region in Kerala was achieved through SDS-PAGE and RP-HPLC followed by Q-TOF LC-MS/MS analysis, incorporating PEAKS and Novor assisted de novo sequencing methodologies. A total of 115 proteins distributed across 17 different enzymatic and non-enzymatic venom protein families were identified through conventional and 39 peptides through homology-driven proteomics approaches. Fourteen peptides derived through de novo complements the Mascot data indicating the importance of homology-driven approaches in improving protein sequence information. Among the protein families identified, glutathione peroxidase and endonuclease were reported for the first time in the Indian cobra venom. Immunological cross-reactivity assessed using Indian polyvalent antivenoms suggested that VINS showed better EC
    MeSH term(s) Animals ; Antivenins ; Chromatography, Liquid ; Elapid Venoms/analysis ; Naja naja/metabolism ; Proteome ; Tandem Mass Spectrometry
    Chemical Substances Antivenins ; Elapid Venoms ; Proteome
    Language English
    Publishing date 2022-01-28
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2022.106324
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.136: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Venomics and antivenomics of Indian spectacled cobra (Naja naja) from the Western Ghats

    Vanuopadath, Muralidharan / Raveendran, Dileepkumar / Nair, Bipin Gopalakrishnan / Nair, Sudarslal Sadasivan

    Acta tropica. 2022 Apr., v. 228

    2022  

    Abstract: Venom proteome profiling of Naja naja from the Western Ghats region in Kerala was achieved through SDS-PAGE and RP-HPLC followed by Q-TOF LC-MS/MS analysis, incorporating PEAKS and Novor assisted de novo sequencing methodologies. A total of 115 proteins ... ...

    Abstract Venom proteome profiling of Naja naja from the Western Ghats region in Kerala was achieved through SDS-PAGE and RP-HPLC followed by Q-TOF LC-MS/MS analysis, incorporating PEAKS and Novor assisted de novo sequencing methodologies. A total of 115 proteins distributed across 17 different enzymatic and non-enzymatic venom protein families were identified through conventional and 39 peptides through homology-driven proteomics approaches. Fourteen peptides derived through de novo complements the Mascot data indicating the importance of homology-driven approaches in improving protein sequence information. Among the protein families identified, glutathione peroxidase and endonuclease were reported for the first time in the Indian cobra venom. Immunological cross-reactivity assessed using Indian polyvalent antivenoms suggested that VINS showed better EC₅₀ (2.48 µg/mL) value than that of PSAV (6.04 µg/mL) and Virchow (6.03 µg/mL) antivenoms. Western blotting experiments indicated that all the antivenoms elicited poor binding specificities, especially towards low molecular mass proteins. Second-generation antivenomics studies revealed that VINS antivenom was less efficient to detect many low molecular mass proteins such as three-finger toxins and Kunitz-type serine protease Inhibitors. Taken together, the present study enabled a large-scale characterization of the venom proteome of Naja naja from the Western Ghats and emphasized the need for developing more efficient antivenoms.
    Keywords Naja naja ; amino acid sequences ; antivenoms ; cobra venoms ; cross reaction ; glutathione peroxidase ; molecular weight ; peptides ; polyacrylamide gel electrophoresis ; proteome ; proteomics ; serine proteinases ; India
    Language English
    Dates of publication 2022-04
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 210415-5
    ISSN 1873-6254 ; 0001-706X
    ISSN (online) 1873-6254
    ISSN 0001-706X
    DOI 10.1016/j.actatropica.2022.106324
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 52/75: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Delineating the venom toxin arsenal of Malabar pit viper (Trimeresurus malabaricus) from the Western Ghats of India and evaluating its immunological cross-reactivity and in vitro cytotoxicity

    Vanuopadath, Muralidharan / Shaji, Sanu Korumadathil / Raveendran, Dileepkumar / Nair, Bipin Gopalakrishnan / Nair, Sudarslal Sadasivan

    International journal of biological macromolecules. 2020 Apr. 01, v. 148

    2020  

    Abstract: The venom protein components of Malabar pit viper (Trimeresurus malabaricus) were identified by combining SDS-PAGE and ion-exchange chromatography pre-fractionation techniques with LC-MS/MS incorporating Novor and PEAKS-assisted de novo sequencing ... ...

    Abstract The venom protein components of Malabar pit viper (Trimeresurus malabaricus) were identified by combining SDS-PAGE and ion-exchange chromatography pre-fractionation techniques with LC-MS/MS incorporating Novor and PEAKS-assisted de novo sequencing strategies. Total 97 proteins that belong to 16 protein families such as L-amino acid oxidase, metalloprotease, serine protease, phospholipase A2, 5′-nucleotidase, C-type lectins/snaclecs and disintegrin were recognized from the venom of a single exemplar species. Of the 97 proteins, eighteen were identified through de novo approaches. Immunological cross-reactivity assessed through ELISA and western blot indicate that the Indian antivenoms binds less effectively to Malabar pit viper venom components compared to that of Russell's viper venom. The in vitro cell viability assays suggest that compared to the normal cells, MPV venom induces concentration dependent cell death in various cancer cells. Moreover, crude venom resulted in chromatin condensation and apoptotic bodies implying the induction of apoptosis. Taken together, the present study enabled in dissecting the venom proteome of Trimeresurus malabaricus and revealed the immuno-cross-reactivity profiles of commercially available Indian polyvalent antivenoms that, in turn, is expected to provide valuable insights on the need in improving antivenom preparations against its bite.
    Keywords Daboia russelii ; L-amino-acid oxidase ; Trimeresurus ; Western blotting ; amino acids ; antivenoms ; apoptosis ; cell viability ; chromatin ; cross reaction ; cytotoxicity ; enzyme-linked immunosorbent assay ; ion exchange chromatography ; lectins ; metalloproteinases ; neoplasm cells ; neoplasms ; phospholipase A2 ; phospholipases ; polyacrylamide gel electrophoresis ; proteome ; serine proteinases ; tandem mass spectrometry ; viability assays ; viper venoms ; India
    Language English
    Dates of publication 2020-0401
    Size p. 1029-1045.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2020.01.226
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Delineating the venom toxin arsenal of Malabar pit viper (Trimeresurus malabaricus) from the Western Ghats of India and evaluating its immunological cross-reactivity and in vitro cytotoxicity.

    Vanuopadath, Muralidharan / Shaji, Sanu Korumadathil / Raveendran, Dileepkumar / Nair, Bipin Gopalakrishnan / Nair, Sudarslal Sadasivan

    International journal of biological macromolecules

    2020  Volume 148, Page(s) 1029–1045

    Abstract: The venom protein components of Malabar pit viper (Trimeresurus malabaricus) were identified by combining SDS-PAGE and ion-exchange chromatography pre-fractionation techniques with LC-MS/MS incorporating Novor and PEAKS-assisted de novo sequencing ... ...

    Abstract The venom protein components of Malabar pit viper (Trimeresurus malabaricus) were identified by combining SDS-PAGE and ion-exchange chromatography pre-fractionation techniques with LC-MS/MS incorporating Novor and PEAKS-assisted de novo sequencing strategies. Total 97 proteins that belong to 16 protein families such as L-amino acid oxidase, metalloprotease, serine protease, phospholipase A
    MeSH term(s) 5'-Nucleotidase/chemistry ; Animals ; Antivenins/chemistry ; Apoptosis/drug effects ; Cell Line ; Cell Survival/drug effects ; Chromatography, High Pressure Liquid ; Crotalid Venoms/analysis ; Crotalid Venoms/enzymology ; Crotalid Venoms/toxicity ; Humans ; India ; L-Amino Acid Oxidase/chemistry ; Lectins, C-Type/chemistry ; Metalloproteases/chemistry ; Mice ; Phospholipases A2/chemistry ; Proteome/chemistry ; Daboia ; Serine Proteases/chemistry ; Tandem Mass Spectrometry ; Trimeresurus
    Chemical Substances Antivenins ; Crotalid Venoms ; Lectins, C-Type ; Proteome ; L-Amino Acid Oxidase (EC 1.4.3.2) ; Phospholipases A2 (EC 3.1.1.4) ; 5'-Nucleotidase (EC 3.1.3.5) ; Metalloproteases (EC 3.4.-) ; Serine Proteases (EC 3.4.-)
    Language English
    Publishing date 2020-01-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2020.01.226
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Ethnomedicinal plants used for snake envenomation by folk traditional practitioners from Kallar forest region of South Western Ghats, Kerala, India.

    Sulochana, Anaswara Krishnan / Raveendran, Dileepkumar / Krishnamma, Anoop Pushkaran / Oommen, Oommen V

    Journal of intercultural ethnopharmacology

    2014  Volume 4, Issue 1, Page(s) 47–51

    Abstract: Background: The traditional medicinal systems of Indian folklore abundantly use medicinal plants or its derivatives for the treatment of snakebites. However, this traditional knowledge is on the verge of extinction, and there is an immediate necessity ... ...

    Abstract Background: The traditional medicinal systems of Indian folklore abundantly use medicinal plants or its derivatives for the treatment of snakebites. However, this traditional knowledge is on the verge of extinction, and there is an immediate necessity to conserve this oral traditional knowledge primarily by proper documentation and scientific authentication. The present ethno botanical study carried out among the folk medicine practitioners in the rural settle mental areas of Kallar forest region of southern Kerala, aims to document the folk herbal knowledge particularly for snake envenomation.
    Materials and methods: The survey was conducted during the period of June 2012-July 2013 in the rural and forest settlement areas of Kallar in the Thiruvananthapuram district of Kerala. Direct observation and oral communications with local folk medicine practitioners in this region were adopted to collect valid information regarding the herbal formulations used to treat snake bite patients.
    Results: The study enumerates a list of 24 plant species belonging to seventeen families with anti-venomous potential. The scientific, vernacular and family names of these plants, along with the part used and their application modes are also enumerated in this communication.
    Conclusions: Plants are believed to be potent snake bite antidotes from centuries back, and knowledge about the use of plants is strictly conserved among tribes through generations without recorded data. It is the need of the hour to document these old drug formulations and is the cardinal responsibility of the scientific community to validate it and come up with new potent drug molecule for the benefit of snake bite victims.
    Language English
    Publishing date 2014-11-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2721488-6
    ISSN 2146-8397
    ISSN 2146-8397
    DOI 10.5455/jice.20141010122750
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Mass spectrometry-assisted venom profiling of Hypnale hypnale found in the Western Ghats of India incorporating de novo sequencing approaches.

    Vanuopadath, Muralidharan / Sajeev, Nithin / Murali, Athira Radhamony / Sudish, Nayana / Kangosseri, Nithya / Sebastian, Ivy Rose / Jain, Nidhi Dalpatraj / Pal, Amit / Raveendran, Dileepkumar / Nair, Bipin Gopalakrishnan / Nair, Sudarslal Sadasivan

    International journal of biological macromolecules

    2018  Volume 118, Issue Pt B, Page(s) 1736–1746

    Abstract: Hypnale hypnale (hump-nosed pit viper) is considered to be one among the medically important venomous snake species of India and Sri Lanka. In the present study, venom proteome profiling of a single Hypnale hypnale from Western Ghats of India was ... ...

    Abstract Hypnale hypnale (hump-nosed pit viper) is considered to be one among the medically important venomous snake species of India and Sri Lanka. In the present study, venom proteome profiling of a single Hypnale hypnale from Western Ghats of India was achieved using SDS-PAGE based protein separation followed by LC-MS/MS analysis. The identities of the proteins that were not established using the Mascot search were determined through de novo sequencing tools such as Novor followed by MS-BLAST based sequence similarity search algorithm and PEAKS proteomics software. The combined proteomics analysis revealed a total of 37 proteins belonging to nine different snake venom families, in which 7 proteins were exclusively identified through de novo strategies. The enzymatic and non-enzymatic venom protein families identified include serine proteases, metalloproteases, phospholipase A
    MeSH term(s) Amino Acid Sequence ; Chromatography, Liquid ; India ; Proteome ; Proteomics/methods ; Snake Venoms/analysis ; Snake Venoms/chemistry ; Tandem Mass Spectrometry
    Chemical Substances Proteome ; Snake Venoms
    Language English
    Publishing date 2018-07-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2018.07.016
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top