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Article ; Online: A grave injustice.

Raven, G

2007 Volume 202, Issue 2, Page(s) 55–56

MeSH term(s)	Dentists/economics ; Dentists/legislation & jurisprudence ; Dentists/psychology ; HIV Infections/economics ; HIV Infections/prevention & control ; HIV Infections/psychology ; HIV Infections/transmission ; Humans ; Infectious Disease Transmission, Professional-to-Patient/prevention & control ; Social Isolation/psychology ; Unemployment ; United Kingdom
Language	English
Publishing date	2007-01-27
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	218090-x
ISSN	1476-5373 ; 0007-0610
ISSN (online)	1476-5373
ISSN	0007-0610
DOI	10.1038/bdj.2007.40
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Article: Consumers and healthcare.

Raven, G

British dental journal

2003 Volume 195, Issue 11, Page(s) 619–620

MeSH term(s)	Advertising as Topic/ethics ; Decision Making ; Dental Care/ethics ; Dentist-Patient Relations ; Ethics, Dental ; Humans ; Patient Participation ; Personal Autonomy
Language	English
Publishing date	2003-12-06
Publishing country	England
Document type	Comment ; Letter
ZDB-ID	218090-x
ISSN	1476-5373 ; 0007-0610
ISSN (online)	1476-5373
ISSN	0007-0610
DOI	10.1038/sj.bdj.4810799
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Article: Uncemented crowns.

Raven, G

British dental journal

2002 Volume 192, Issue 7, Page(s) 362

MeSH term(s)	Cementation ; Crowns ; Humans ; Prosthesis Fitting
Language	English
Publishing date	2002-04-13
Publishing country	England
Document type	Comment ; Letter
ZDB-ID	218090-x
ISSN	1476-5373 ; 0007-0610
ISSN (online)	1476-5373
ISSN	0007-0610
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Article: Professional responsibility.

Raven, G

British dental journal

2002 Volume 192, Issue 12, Page(s) 668–669

MeSH term(s)	Ethics, Dental ; Humans ; Public Opinion ; Social Responsibility ; United Kingdom
Language	English
Publishing date	2002-06-29
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	218090-x
ISSN	1476-5373 ; 0007-0610
ISSN (online)	1476-5373
ISSN	0007-0610
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Article ; Online: Notochord isolation using laser capture microdissection.

Santegoeds, R G C / Yakkioui, Y / Jahanshahi, A / Raven, G / Van Overbeeke, J J / Herrler, A / Temel, Y

Journal of chemical neuroanatomy

2016 Volume 80, Page(s) 37–43

Abstract: Background: Chordoma are malignant tumors of the axial skeleton, which arise from remnants of the notochord. The Notochord (chorda dorsalis) is an essential embryonic structure involved in the development of the nervous system and axial skeleton. ... ...

Abstract	Background: Chordoma are malignant tumors of the axial skeleton, which arise from remnants of the notochord. The Notochord (chorda dorsalis) is an essential embryonic structure involved in the development of the nervous system and axial skeleton. Therefore, the notochord seems to be the most biologically relevant control tissue to study chordoma in molecular biology research. Nevertheless, up to now mainly different tissues but not the notochord have been used as control for chordoma, due to difficulty of isolating notochordal tissue. Here, we describe a fast and precise method of isolating notochordal cells. Methods: Examination of human fetuses, with a gestation of 9, 11 and 13 weeks, using (immuno)histochemical methods was performed. To isolate pure notochord cells for further molecular biology investigation five flash frozen fetuses between 9 and 10 weeks of gestation were dissected by microtome slicing. Thereafter pure notochord cells for further molecular biology investigation where harvested by using laser capture microdissection (LCM). RNA was extracted from these samples and used in quantitative PCR. Results: This study illustrates notochord of embryonic spines in three different stages of gestation (9-11-13 weeks). Immunohistochemical staining with brachyury showed strong staining of the notochord, but also weak staining of the intervertebral disc and vertebral body. LCM of notochord slices and subsequent total RNA extraction resulted in a good yield of total RNA. qPCR analysis of two housekeeping genes confirmed the quality of the RNA. Conclusion: LCM is a fast and precise method to isolate notochord and the quality and yield RNA extracted from this tissue is sufficient for qPCR analysis. Therefore early embryo notochord isolated by LCM is suggested to be the gold standard for future research in chordoma development, classification and diagnosis.
MeSH term(s)	Female ; Fetal Development ; Fetus/anatomy & histology ; Gestational Age ; Humans ; Immunohistochemistry ; Laser Capture Microdissection/methods ; Notochord/anatomy & histology ; Pregnancy ; RNA/biosynthesis ; RNA/genetics ; Real-Time Polymerase Chain Reaction ; Spine/embryology
Chemical Substances	RNA (63231-63-0)
Language	English
Publishing date	2016-12-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	639443-7
ISSN	1873-6300 ; 0891-0618
ISSN (online)	1873-6300
ISSN	0891-0618
DOI	10.1016/j.jchemneu.2016.12.004
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Article ; Online: Probing the interaction of the potassium channel modulating KCNE1 in lipid bilayers via solid-state NMR spectroscopy.

Zhang, Rongfu / Sahu, Indra D / Comer, Raven G / Maltsev, Sergey / Dabney-Smith, Carole / Lorigan, Gary A

Magnetic resonance in chemistry : MRC

2017 Volume 55, Issue 8, Page(s) 754–758

Abstract: KCNE1 is known to modulate the voltage-gated potassium channel α subunit KCNQ1 to generate slowly activating potassium currents. This potassium channel is essential for the cardiac action potential that mediates a heartbeat as well as the potassium ion ... ...

Abstract	KCNE1 is known to modulate the voltage-gated potassium channel α subunit KCNQ1 to generate slowly activating potassium currents. This potassium channel is essential for the cardiac action potential that mediates a heartbeat as well as the potassium ion homeostasis in the inner ear. Therefore, it is important to know the structure and dynamics of KCNE1 to better understand its modulatory role. Previously, the Sanders group solved the three-dimensional structure of KCNE1 in LMPG micelles, which yielded a better understanding of this KCNQ1/KCNE1 channel activity. However, research in the Lorigan group showed different structural properties of KCNE1 when incorporated into POPC/POPG lipid bilayers as opposed to LMPG micelles. It is hence necessary to study the structure of KCNE1 in a more native-like environment such as multi-lamellar vesicles. In this study, the dynamics of lipid bilayers upon incorporation of the membrane protein KCNE1 were investigated using
MeSH term(s)	Amino Acid Sequence ; Humans ; Kinetics ; Lipid Bilayers/chemistry ; Magnetic Resonance Spectroscopy ; Micelles ; Potassium Channels, Voltage-Gated/chemistry
Chemical Substances	KCNE1 protein, human ; Lipid Bilayers ; Micelles ; Potassium Channels, Voltage-Gated
Language	English
Publishing date	2017-08
Publishing country	England
Document type	Journal Article
ZDB-ID	1475029-6
ISSN	1097-458X ; 0749-1581
ISSN (online)	1097-458X
ISSN	0749-1581
DOI	10.1002/mrc.4589
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Article ; Online: Complex patterns of ADAM12 mRNA and protein splice variants in the human placenta.

Kokozidou, M / Drewlo, S / Bartz, C / Raven, G / Brandenburg, L O / Wruck, C J / Pufe, T

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

2011 Volume 193, Issue 2, Page(s) 142–148

Abstract: Aims: Trophoblast fusion in the placenta is prerequisite to successful pregnancy and the pathological conditions related to it. The presence of syncytin-1, is not sufficient to explain the complete event and ADAM12 is a major co-player candidate. Via ... ...

Abstract	Aims: Trophoblast fusion in the placenta is prerequisite to successful pregnancy and the pathological conditions related to it. The presence of syncytin-1, is not sufficient to explain the complete event and ADAM12 is a major co-player candidate. Via differential splicing, the ADAM12 gene produces a short and a long form, being the ADAM12-S and the ADAM12-L respectively. Methods and results: We investigated the localisation of both variants in the human placenta using whole mount in situ hybridisation, immunohistochemistry and Northern blotting in 1st (n=8) and 3rd (n=8) trimester placentae and in the case of NB in several cell lines. In Northern blotting, 1st and 3rd trimester placentae were positive for the ADAM12-S and Bewo, 293HEK, JAR, leucocytes, macrophages, 1st and 3rd trimester placentae were positive for ADAM12-L. In whole mount in situ hybridisation, the 1st and 3rd trimester placental syncytium was positive for both variants. In immunohistochemistry, ADAM12-L localised in the cytotrophoblast of both 1st and 3rd trimester placentae, while ADAM12-S localised in the complete syncytium, often including the cytotrophoblast. Conclusion: The different localisation of ADAM12-S and ADAM12-L indicates a possible different role making ADAM12-L a candidate for the fusion event, while the syncytial localisation of the ADAM12-S makes it a candidate for cell-cell and cell-matrix interactions between the placental syncytium and the maternal interface.
MeSH term(s)	ADAM Proteins/genetics ; ADAM12 Protein ; Female ; Humans ; Membrane Proteins/genetics ; Placenta/physiology ; Pregnancy ; Protein Isoforms/genetics ; RNA, Messenger/genetics ; Tissue Distribution
Chemical Substances	Membrane Proteins ; Protein Isoforms ; RNA, Messenger ; ADAM Proteins (EC 3.4.24.-) ; ADAM12 Protein (EC 3.4.24.-) ; ADAM12 protein, human (EC 3.4.24.-)
Language	English
Publishing date	2011-03
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1106738-x
ISSN	1618-0402 ; 0940-9602
ISSN (online)	1618-0402
ISSN	0940-9602
DOI	10.1016/j.aanat.2010.12.002
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Article ; Online: Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3.

Siroky, Brian J / Kleene, Nancy K / Kleene, Steven J / Varnell, Charles D / Comer, Raven G / Liu, Jialiu / Lu, Lu / Pachciarz, Nolan W / Bissler, John J / Dixon, Bradley P

American journal of physiology. Renal physiology

2017 Volume 312, Issue 4, Page(s) F791–F805

Abstract: Primary cilia sense environmental conditions, including osmolality, but whether cilia participate in the osmotic response in renal epithelial cells is not known. The transient receptor potential (TRP) channels TRPV4 and TRPM3 are osmoresponsive. TRPV4 ... ...

Abstract	Primary cilia sense environmental conditions, including osmolality, but whether cilia participate in the osmotic response in renal epithelial cells is not known. The transient receptor potential (TRP) channels TRPV4 and TRPM3 are osmoresponsive. TRPV4 localizes to cilia in certain cell types, while renal subcellular localization of TRPM3 is not known. We hypothesized that primary cilia are required for maximal activation of the osmotic response of renal epithelial cells and that ciliary TRPM3 and TRPV4 mediate that response. Ciliated [murine epithelial cells from the renal inner medullary collecting duct (mIMCD-3) and 176-5] and nonciliated (176-5Δ) renal cells expressed
MeSH term(s)	Animals ; CRISPR-Cas Systems ; Cell Line ; Cilia/metabolism ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; GABA Plasma Membrane Transport Proteins/genetics ; GABA Plasma Membrane Transport Proteins/metabolism ; Gene Editing ; Hydroxyprostaglandin Dehydrogenases/genetics ; Hydroxyprostaglandin Dehydrogenases/metabolism ; Kidney Tubules, Collecting/drug effects ; Kidney Tubules, Collecting/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Osmoregulation/drug effects ; Osmotic Pressure/drug effects ; Saline Solution, Hypertonic/pharmacology ; Signal Transduction ; TRPM Cation Channels/genetics ; TRPM Cation Channels/metabolism ; TRPV Cation Channels/genetics ; TRPV Cation Channels/metabolism ; Transfection
Chemical Substances	GABA Plasma Membrane Transport Proteins ; Saline Solution, Hypertonic ; Slc6a12 protein, mouse ; TRPM Cation Channels ; TRPM3 protein, mouse ; TRPV Cation Channels ; Trpv4 protein, mouse ; Hydroxyprostaglandin Dehydrogenases (EC 1.1.1.-) ; prostaglandin-F synthase (EC 1.1.1.188)
Language	English
Publishing date	2017-01-25
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	603837-2
ISSN	1522-1466 ; 0363-6127
ISSN (online)	1522-1466
ISSN	0363-6127
DOI	10.1152/ajprenal.00465.2015
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Article: Characterizing the structure of lipodisq nanoparticles for membrane protein spectroscopic studies.

Zhang, Rongfu / Sahu, Indra D / Liu, Lishan / Osatuke, Anna / Comer, Raven G / Dabney-Smith, Carole / Lorigan, Gary A

Biochimica et biophysica acta

2014 Volume 1848, Issue 1 Pt B, Page(s) 329–333

Abstract: Membrane protein spectroscopic studies are challenging due to the difficulty introduced in preparing homogenous and functional hydrophobic proteins incorporated into a lipid bilayer system. Traditional membrane mimics such as micelles or liposomes have ... ...

Abstract	Membrane protein spectroscopic studies are challenging due to the difficulty introduced in preparing homogenous and functional hydrophobic proteins incorporated into a lipid bilayer system. Traditional membrane mimics such as micelles or liposomes have proved to be powerful in solubilizing membrane proteins for biophysical studies, however, several drawbacks have limited their applications. Recently, a nanosized complex termed lipodisq nanoparticles was utilized as an alternative membrane mimic to overcome these caveats by providing a homogeneous lipid bilayer environment. Despite all the benefits that lipodisq nanoparticles could provide to enhance the biophysical studies of membrane proteins, structural characterization in different lipid compositions that closely mimic the native membrane environment is still lacking. In this study, the formation of lipodisq nanoparticles using different weight ratios of POPC/POPG lipids to SMA polymers was characterized via solid-state nuclear magnetic resonance (SSNMR) spectroscopy and dynamic light scattering (DLS). A critical weight ratio of (1/1.25) for the complete solubilization of POPC/POPG vesicles has been observed and POPC/POPG vesicles turned clear instantaneously upon the addition of the SMA polymer. The size of lipodisq nanoparticles formed from POPC/POPG lipids at this weight ratio of (1/1.25) was found to be about 30 nm in radius. We also showed that upon the complete solubilization of POPC/POPG vesicles by SMA polymers, the average size of the lipodisq nanoparticles is weight ratio dependent, when more SMA polymers were introduced, smaller lipodisq nanoparticles were obtained. The results of this study will be helpful for a variety of biophysical experiments when specific size of lipid disc is required. Further, this study will provide a proper path for researchers working on membrane proteins to obtain pertinent structure and dynamic information in a physiologically relevant membrane mimetic environment.
MeSH term(s)	Lipid Bilayers/chemistry ; Magnetic Resonance Spectroscopy ; Maleates/chemistry ; Membrane Proteins/chemistry ; Nanoparticles/chemistry ; Phosphatidylcholines/chemistry ; Phosphatidylglycerols/chemistry ; Polystyrenes/chemistry
Chemical Substances	Lipid Bilayers ; Maleates ; Membrane Proteins ; Phosphatidylcholines ; Phosphatidylglycerols ; Polystyrenes ; 1-palmitoyl-2-oleoylglycero-3-phosphoglycerol (81490-05-3) ; styrofoam (9003-53-6) ; maleic acid (91XW058U2C) ; 1-palmitoyl-2-oleoylphosphatidylcholine (TE895536Y5)
Language	English
Publishing date	2014-05-20
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbamem.2014.05.008
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Article: Have changes to meat and poultry food safety regulation in Australia affected the prevalence of Salmonella or of salmonellosis?

Sumner, John / Raven, Geoff / Givney, Rod

International journal of food microbiology

2004 Volume 92, Issue 2, Page(s) 199–205

Abstract: During the 1990s, there was radical change in regulation of meat and poultry hygiene in Australia, and Australian Standards were developed for each sector of the meat industry. Systems for industry/government co-regulation and company-employed meat ... ...

Abstract	During the 1990s, there was radical change in regulation of meat and poultry hygiene in Australia, and Australian Standards were developed for each sector of the meat industry. Systems for industry/government co-regulation and company-employed meat inspection were introduced based on company HACCP programs approved and audited by the Controlling Authority. However, in the 5 years since regulatory changes took full effect, rates of salmonellosis have not decreased (surveillance and reporting systems have remained unchanged). Using statistics gathered by the National Enteric Pathogens Surveillance Scheme, an attempt was made to link Salmonella serovars isolated from meat and poultry with those causing salmonellosis. Two periods were studied, 1993/1994, before regulations were introduced, and 2000/2001, when regulations should be having an effect. For red meat, the same serovars were prominent among the top 10 isolates both before and after regulation, and there was little linkage with salmonelloses. For poultry, frequently isolated serovars differed pre- and post-regulation, however, in both periods there was some linkage between serovars isolated from poultry and those causing salmonelloses. Using published and unpublished survey data, it was concluded that there had been improvements in microbiological quality of red meat and poultry over the same timeframe as regulatory changes. That these improvements apparently have not carried through to reduced case-rates for salmonellosis may be due to numerous causes, including lack of control in the food processing, food service and home sectors. The present paper illustrates difficulties faced by governments in measuring public health outcomes of changes to food hygiene regulation.
MeSH term(s)	Animals ; Australia ; Cattle/microbiology ; Consumer Product Safety ; Food Contamination/analysis ; Food Contamination/prevention & control ; Food Handling/methods ; Food Handling/standards ; Food Inspection ; Food Microbiology ; Humans ; Hygiene ; Legislation, Food ; Meat/microbiology ; Meat/standards ; Poultry/microbiology ; Prevalence ; Public Health ; Salmonella/classification ; Salmonella/isolation & purification ; Salmonella/pathogenicity ; Salmonella Infections/epidemiology ; Salmonella Infections/microbiology
Language	English
Publishing date	2004-04-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	87122-9
ISSN	1879-3460 ; 0168-1605
ISSN (online)	1879-3460
ISSN	0168-1605
DOI	10.1016/j.ijfoodmicro.2003.10.003
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