Article ; Online: Dysregulated monocyte-derived macrophage response to Group B
2023 Volume 14, Page(s) 1268804
Abstract: Introduction: Streptococcus agalactiae: Methods: Monocytes from umbilical cord blood of healthy term newborns and from peripheral blood of healthy adult subjects were cultured with M-CSF to induce MDMs. M-CSF-MDMs, GM-CSF- and IFNγ-activated MDMs ... ...
| Abstract | Introduction: Streptococcus agalactiae Methods: Monocytes from umbilical cord blood of healthy term newborns and from peripheral blood of healthy adult subjects were cultured with M-CSF to induce MDMs. M-CSF-MDMs, GM-CSF- and IFNγ-activated MDMs were exposed to GBS COH1, a reference strain for neonatal sepsis. Results: GBS induced a greater release of IL-1β, IL-6, IL-10, IL-12p70 and IL-23 in newborn compared to adult MDMs, while IL-18, IL-21, IL-22, TNF, RANTES/CCL5, MCP-1/CCL2 and IL-8/CXCL8 were released at similar levels. MDM responses to GBS were strongly influenced by conditions of activation and were distinct from those to synthetic bacterial lipopeptides and lipopolysaccharides. Under similar conditions of opsonization, newborn MDMs phagocytosed and killed GBS as efficiently as adult MDMs. Discussion: Altogether, the production of excessive levels of Th1- (IL-12p70), Th17-related (IL-1β, IL-6, IL-23) and anti-inflammatory (IL-10) cytokines is consistent with a dysregulated response to GBS in newborns. The high responsiveness of newborn MDMs may play a role in the progression of GBS infection in newborns, possibly contributing to the development of life-threatening organ dysfunction. |
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| MeSH term(s) | Adult ; Infant, Newborn ; Humans ; Interleukin-10 ; Macrophage Colony-Stimulating Factor ; Interleukin-6 ; Neonatal Sepsis ; Streptococcus agalactiae ; Macrophages ; Interleukin-12 ; Interleukin-23 |
| Chemical Substances | Interleukin-10 (130068-27-8) ; Macrophage Colony-Stimulating Factor (81627-83-0) ; Interleukin-6 ; Interleukin-12 (187348-17-0) ; Interleukin-23 |
| Language | English |
| Publishing date | 2023-11-14 |
| Publishing country | Switzerland |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2606827-8 |
| ISSN | 1664-3224 ; 1664-3224 |
| ISSN (online) | 1664-3224 |
| ISSN | 1664-3224 |
| DOI | 10.3389/fimmu.2023.1268804 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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