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  1. Article ; Online: Multicenter evaluation of the FilmArray Meningitis/Encephalitis assay in a routine setting.

    Maelegheer, Karel / Reynders, Marijke / Floré, Katelijne / Vanacker, Jos / Vanlaere, Elke / Cartuyvels, Reinoud / Raymaekers, Marijke

    Journal of medical microbiology

    2021  Volume 70, Issue 10

    Abstract: Introduction. ...

    Abstract Introduction.
    MeSH term(s) Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Diagnostic Tests, Routine/methods ; Female ; Humans ; Infant ; Infant, Newborn ; Infectious Encephalitis/diagnosis ; Male ; Meningitis/diagnosis ; Middle Aged ; Multiplex Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Young Adult
    Language English
    Publishing date 2021-10-19
    Publishing country England
    Document type Comparative Study ; Journal Article ; Multicenter Study
    ZDB-ID 218356-0
    ISSN 1473-5644 ; 0022-2615
    ISSN (online) 1473-5644
    ISSN 0022-2615
    DOI 10.1099/jmm.0.001438
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 634: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  2. Article ; Online: Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19.

    Boeckmans, Joost / Cartuyvels, Reinoud / Hilkens, Petra / Bruckers, Liesbeth / Magerman, Koen / Waumans, Luc / Raymaekers, Marijke

    Diagnostic microbiology and infectious disease

    2021  Volume 100, Issue 2, Page(s) 115350

    Abstract: Detection of SARS-CoV-2 RNA in nasopharyngeal samples using the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the gold standard for diagnosing COVID-19. Determination of SARS-CoV-2 RNA by rRT-PCR sometimes results in an ... ...

    Abstract Detection of SARS-CoV-2 RNA in nasopharyngeal samples using the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the gold standard for diagnosing COVID-19. Determination of SARS-CoV-2 RNA by rRT-PCR sometimes results in an inconclusive test result due to a high cycle threshold-value. We retrospectively analyzed 30,851 SARS-CoV-2 rRT-PCR test results. Borderline positivity was considered as the presence of ≤25 viral copies per milliliter, while no amplification was considered as a negative test result. Of all test results, 204 were answered as borderline, of which 107 were accompanied by a follow-up test within 96 hours. Of the 107 follow-up samples, 10 (9.35%) were found positive for SARS-CoV-2. COVID-19 symptoms were not predictive for testing positive in the follow-up test. The positive SARS-CoV-2 samples in the follow-up group represented 0.92% of all positive test results, highlighting the need for retesting and increased hygienic measures for borderline SARS-CoV-2 patients [NCT04636294].
    MeSH term(s) COVID-19/diagnosis ; COVID-19 Testing/methods ; Early Diagnosis ; Follow-Up Studies ; Humans ; Real-Time Polymerase Chain Reaction/methods ; Retrospective Studies ; SARS-CoV-2
    Language English
    Publishing date 2021-02-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2021.115350
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1845: Show issues Location:
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  3. Article ; Online: Vertical transmission of SARS-CoV-2 infection and preterm birth.

    Pulinx, Bianca / Kieffer, Davy / Michiels, Ian / Petermans, Stéphanie / Strybol, David / Delvaux, Sigrun / Baldewijns, Marcella / Raymaekers, Marijke / Cartuyvels, Reinoud / Maurissen, Wim

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

    2020  Volume 39, Issue 12, Page(s) 2441–2445

    Abstract: Viral infections are common complications of pregnancy, with a wide range of obstetric and neonatal sequelae. Currently, there are limited data on whether SARS-CoV-2 is vertically transmitted in pregnant women tested positive for the virus. Here we ... ...

    Abstract Viral infections are common complications of pregnancy, with a wide range of obstetric and neonatal sequelae. Currently, there are limited data on whether SARS-CoV-2 is vertically transmitted in pregnant women tested positive for the virus. Here we describe a case of a known SARS-CoV-2-positive woman giving preterm birth to two fetuses with SARS-CoV-2 positive testing in placental tissue and amniotic fluid. The placental histological examinations showed chronic intervillositis and extensive intervillous fibrin depositions with ischemic necrosis of the surrounding villi.
    MeSH term(s) Adult ; Amniotic Fluid/virology ; Betacoronavirus/pathogenicity ; COVID-19 ; Cesarean Section ; Coronavirus Infections/diagnosis ; Coronavirus Infections/pathology ; Coronavirus Infections/transmission ; Coronavirus Infections/virology ; Diabetes, Gestational/diagnosis ; Diabetes, Gestational/pathology ; Diabetes, Gestational/virology ; Female ; Fetal Death ; Fetus ; Humans ; Infectious Disease Transmission, Vertical ; Pandemics ; Placenta/pathology ; Placenta/virology ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/pathology ; Pneumonia, Viral/transmission ; Pneumonia, Viral/virology ; Pregnancy ; Pregnancy Complications, Infectious/diagnosis ; Pregnancy Complications, Infectious/pathology ; Pregnancy Complications, Infectious/virology ; Premature Birth/pathology ; Premature Birth/virology ; SARS-CoV-2 ; Twins, Dizygotic
    Keywords covid19
    Language English
    Publishing date 2020-07-13
    Publishing country Germany
    Document type Case Reports ; Journal Article
    ZDB-ID 603155-9
    ISSN 1435-4373 ; 0934-9723 ; 0722-2211
    ISSN (online) 1435-4373
    ISSN 0934-9723 ; 0722-2211
    DOI 10.1007/s10096-020-03964-y
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    Zs.A 1732: Show issues Location:
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  4. Article: Vertical transmission of SARS-CoV-2 infection and preterm birth

    Pulinx, Bianca / Kieffer, Davy / Michiels, Ian / Petermans, Stéphanie / Strybol, David / Delvaux, Sigrun / Baldewijns, Marcella / Raymaekers, Marijke / Cartuyvels, Reinoud / Maurissen, Wim

    Eur J Clin Microbiol Infect Dis

    Abstract: Viral infections are common complications of pregnancy, with a wide range of obstetric and neonatal sequelae. Currently, there are limited data on whether SARS-CoV-2 is vertically transmitted in pregnant women tested positive for the virus. Here we ... ...

    Abstract Viral infections are common complications of pregnancy, with a wide range of obstetric and neonatal sequelae. Currently, there are limited data on whether SARS-CoV-2 is vertically transmitted in pregnant women tested positive for the virus. Here we describe a case of a known SARS-CoV-2-positive woman giving preterm birth to two fetuses with SARS-CoV-2 positive testing in placental tissue and amniotic fluid. The placental histological examinations showed chronic intervillositis and extensive intervillous fibrin depositions with ischemic necrosis of the surrounding villi.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #641312
    Database COVID19

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  5. Article ; Online: Vertical transmission of SARS-CoV-2 infection and preterm birth

    Pulinx, Bianca / Kieffer, Davy / Michiels, Ian / Petermans, Stéphanie / Strybol, David / Delvaux, Sigrun / Baldewijns, Marcella / Raymaekers, Marijke / Cartuyvels, Reinoud / Maurissen, Wim

    European Journal of Clinical Microbiology & Infectious Diseases ; ISSN 0934-9723 1435-4373

    2020  

    Keywords Microbiology (medical) ; Infectious Diseases ; General Medicine ; covid19
    Language English
    Publisher Springer Science and Business Media LLC
    Publishing country us
    Document type Article ; Online
    DOI 10.1007/s10096-020-03964-y
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Checklist for optimization and validation of real-time PCR assays.

    Raymaekers, Marijke / Smets, Rita / Maes, Brigitte / Cartuyvels, Reinoud

    Journal of clinical laboratory analysis

    2009  Volume 23, Issue 3, Page(s) 145–151

    Abstract: Real-time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in-house developed molecular diagnostic methods are ...

    Abstract Real-time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in-house developed molecular diagnostic methods are scare. Therefore, we propose a practical guiding principle for the optimization and validation of real-time PCR assays. Based on literature, existing guidelines, and personal experience, we created a checklist that can be used in different steps of the development and validation process of commercial and in-house developed real-time PCR assays. Furthermore, determination of target values and reproducibility of internal quality controls are included, which allows a statistical follow-up of the performance of the assay. Recently, we used this checklist for the development of various qualitative and quantitative assays for microbiological and hematological applications, for which accreditation according to ISO 15189:2007 was obtained. In our experience, the use of the proposed guidelines leads to a more efficient and standardized optimization and validation. Ultimately, this results in reliable and robust molecular diagnostics. The proposed checklist is independent of environment, equipment, and specific applications and can be used in other laboratories. A worldwide consensus on this kind of checklist should be aimed at.
    MeSH term(s) Analytic Sample Preparation Methods ; Calibration ; Data Interpretation, Statistical ; Genes/genetics ; Molecular Diagnostic Techniques/standards ; Oligonucleotide Probes/genetics ; Polymerase Chain Reaction/standards ; Quality Control ; Reproducibility of Results
    Chemical Substances Oligonucleotide Probes
    Language English
    Publishing date 2009-05-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 645095-7
    ISSN 1098-2825 ; 0887-8013
    ISSN (online) 1098-2825
    ISSN 0887-8013
    DOI 10.1002/jcla.20307
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    Zs.A 2471: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article: Nationwide Harmonization Effort for Semi-Quantitative Reporting of SARS-CoV-2 PCR Test Results in Belgium

    Cuypers, Lize / Bode, Jannes / Beuselinck, Kurt / Laenen, Lies / Dewaele, Klaas / Janssen, Reile / Capron, Arnaud / Lafort, Yves / Paridaens, Henry / Bearzatto, Bertrand / Cauchie, Mathieu / Huwart, Aline / Degosserie, Jonathan / Fagnart, Olivier / Overmeire, Yarah / Rouffiange, Arlette / Vandecandelaere, Ilse / Deffontaine, Marine / Pilate, Thomas /
    Yin, Nicolas / Micalessi, Isabel / Roisin, Sandrine / Moons, Veronique / Reynders, Marijke / Steyaert, Sophia / Henin, Coralie / Lazarova, Elena / Obbels, Dagmar / Dufrasne, François E. / Pirenne, Hendri / Schepers, Raf / Collin, Anaëlle / Verhasselt, Bruno / Gillet, Laurent / Jonckheere, Stijn / Van Lint, Philippe / Van den Poel, Bea / Van der Beken, Yolien / Stojkovic, Violeta / Garrino, Maria-Grazia / Segers, Hannah / Vos, Kevin / Godefroid, Maaike / Pede, Valerie / Nollet, Friedel / Claes, Vincent / Verschraegen, Inge / Bogaerts, Pierre / Van Gysel, Marjan / Leurs, Judith / Saegeman, Veroniek / Soetens, Oriane / Vanhee, Merijn / Schiettekatte, Gilberte / Huyghe, Evelyne / Martens, Steven / Lemmens, Ann / Nailis, Heleen / Laffineur, Kim / Steensels, Deborah / Vanlaere, Elke / Gras, Jérémie / Roussel, Gatien / Gijbels, Koenraad / Boudewijns, Michael / Sion, Catherine / Achtergael, Wim / Maurissen, Wim / Iliano, Luc / Chantrenne, Marianne / Vanheule, Geert / Flies, Reinoud / Hougardy, Nicolas / Berth, Mario / Verbeke, Vanessa / Morent, Robin / Vankeerberghen, Anne / Bontems, Sébastien / Kehoe, Kaat / Schallier, Anneleen / Ho, Giang / Bafort, Kristof / Raymaekers, Marijke / Pypen, Yolande / Heinrichs, Amelie / Schuermans, Wim / Cuigniez, Dominique / Lali, Salah Eddine / Drieghe, Stefanie / Ory, Dieter / Le Mercier, Marie / Van Laethem, Kristel / Thoelen, Inge / Vandamme, Sarah / Mansoor, Iqbal / Vael, Carl / De Sloovere, Maxime / Declerck, Katrien / Dequeker, Elisabeth / Desmet, Stefanie / Maes, Piet / Lagrou, Katrien / André, Emmanuel

    Viruses. 2022 June 14, v. 14, no. 6

    2022  

    Abstract: From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to combat the pandemic. Considering the dynamic of viral shedding during the ... ...

    Abstract From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to combat the pandemic. Considering the dynamic of viral shedding during the course of infection, the demand to report cycle threshold (Ct) values rapidly emerged. As Ct values can be affected by a number of factors, we considered that harmonization of semi-quantitative PCR results across laboratories would avoid potential divergent interpretations, particularly in the absence of clinical or serological information. A proposal to harmonize reporting of test results was drafted by the National Reference Centre (NRC) UZ/KU Leuven, distinguishing four categories of positivity based on RNA copies/mL. Pre-quantified control material was shipped to 124 laboratories with instructions to setup a standard curve to define thresholds per assay. For each assay, the mean Ct value and corresponding standard deviation was calculated per target gene, for the three concentrations (10⁷, 10⁵ and 10³ copies/mL) that determine the classification. The results of 17 assays are summarized. This harmonization effort allowed to ensure that all Belgian laboratories would report positive PCR results in the same semi-quantitative manner to clinicians and to the national database which feeds contact tracing interventions.
    Keywords RNA ; Severe acute respiratory syndrome coronavirus 2 ; databases ; genes ; pandemic ; standard deviation ; Belgium
    Language English
    Dates of publication 2022-0614
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061294
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Nationwide Harmonization Effort for Semi-Quantitative Reporting of SARS-CoV-2 PCR Test Results in Belgium.

    Cuypers, Lize / Bode, Jannes / Beuselinck, Kurt / Laenen, Lies / Dewaele, Klaas / Janssen, Reile / Capron, Arnaud / Lafort, Yves / Paridaens, Henry / Bearzatto, Bertrand / Cauchie, Mathieu / Huwart, Aline / Degosserie, Jonathan / Fagnart, Olivier / Overmeire, Yarah / Rouffiange, Arlette / Vandecandelaere, Ilse / Deffontaine, Marine / Pilate, Thomas /
    Yin, Nicolas / Micalessi, Isabel / Roisin, Sandrine / Moons, Veronique / Reynders, Marijke / Steyaert, Sophia / Henin, Coralie / Lazarova, Elena / Obbels, Dagmar / Dufrasne, François E / Pirenne, Hendri / Schepers, Raf / Collin, Anaëlle / Verhasselt, Bruno / Gillet, Laurent / Jonckheere, Stijn / Van Lint, Philippe / Van den Poel, Bea / Van der Beken, Yolien / Stojkovic, Violeta / Garrino, Maria-Grazia / Segers, Hannah / Vos, Kevin / Godefroid, Maaike / Pede, Valerie / Nollet, Friedel / Claes, Vincent / Verschraegen, Inge / Bogaerts, Pierre / Van Gysel, Marjan / Leurs, Judith / Saegeman, Veroniek / Soetens, Oriane / Vanhee, Merijn / Schiettekatte, Gilberte / Huyghe, Evelyne / Martens, Steven / Lemmens, Ann / Nailis, Heleen / Laffineur, Kim / Steensels, Deborah / Vanlaere, Elke / Gras, Jérémie / Roussel, Gatien / Gijbels, Koenraad / Boudewijns, Michael / Sion, Catherine / Achtergael, Wim / Maurissen, Wim / Iliano, Luc / Chantrenne, Marianne / Vanheule, Geert / Flies, Reinoud / Hougardy, Nicolas / Berth, Mario / Verbeke, Vanessa / Morent, Robin / Vankeerberghen, Anne / Bontems, Sébastien / Kehoe, Kaat / Schallier, Anneleen / Ho, Giang / Bafort, Kristof / Raymaekers, Marijke / Pypen, Yolande / Heinrichs, Amelie / Schuermans, Wim / Cuigniez, Dominique / Lali, Salah Eddine / Drieghe, Stefanie / Ory, Dieter / Le Mercier, Marie / Van Laethem, Kristel / Thoelen, Inge / Vandamme, Sarah / Mansoor, Iqbal / Vael, Carl / De Sloovere, Maxime / Declerck, Katrien / Dequeker, Elisabeth / Desmet, Stefanie / Maes, Piet / Lagrou, Katrien / André, Emmanuel

    Viruses

    2022  Volume 14, Issue 6

    Abstract: From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to combat the pandemic. Considering the dynamic of viral shedding during the ... ...

    Abstract From early 2020, a high demand for SARS-CoV-2 tests was driven by several testing indications, including asymptomatic cases, resulting in the massive roll-out of PCR assays to combat the pandemic. Considering the dynamic of viral shedding during the course of infection, the demand to report cycle threshold (Ct) values rapidly emerged. As Ct values can be affected by a number of factors, we considered that harmonization of semi-quantitative PCR results across laboratories would avoid potential divergent interpretations, particularly in the absence of clinical or serological information. A proposal to harmonize reporting of test results was drafted by the National Reference Centre (NRC) UZ/KU Leuven, distinguishing four categories of positivity based on RNA copies/mL. Pre-quantified control material was shipped to 124 laboratories with instructions to setup a standard curve to define thresholds per assay. For each assay, the mean Ct value and corresponding standard deviation was calculated per target gene, for the three concentrations (10
    MeSH term(s) Belgium/epidemiology ; COVID-19/diagnosis ; COVID-19/epidemiology ; Humans ; Pandemics ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2/genetics
    Language English
    Publishing date 2022-06-14
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061294
    Database MEDical Literature Analysis and Retrieval System OnLINE

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