Article: Novel markers of gonadectomy-induced adrenocortical neoplasia in the mouse and ferret
Molecular and Cellular Endocrinology. 2015 Jan. 05, v. 399
2015
Abstract: Gonadectomy (GDX) induces sex steroid-producing adrenocortical tumors in certain mouse strains and in the domestic ferret. Transcriptome analysis and DNA methylation mapping were used to identify novel genetic and epigenetic markers of GDX-induced ... ...
| Abstract | Gonadectomy (GDX) induces sex steroid-producing adrenocortical tumors in certain mouse strains and in the domestic ferret. Transcriptome analysis and DNA methylation mapping were used to identify novel genetic and epigenetic markers of GDX-induced adrenocortical neoplasia in female DBA/2J mice. Markers were validated using a combination of laser capture microdissection, quantitative RT-PCR, in situ hybridization, and immunohistochemistry. Microarray expression profiling of whole adrenal mRNA from ovariectomized vs. intact mice demonstrated selective upregulation of gonadal-like genes including Spinlw1 and Insl3 in GDX-induced adrenocortical tumors of the mouse. A complementary candidate gene approach identified Foxl2 as another gonadal-like marker expressed in GDX-induced neoplasms of the mouse and ferret. That both âmale-specificâ (Spinlw1) and âfemale-specificâ (Foxl2) markers were identified is noteworthy and implies that the neoplasms exhibit mixed characteristics of male and female gonadal somatic cells. Genome-wide methylation analysis showed that two genes with hypomethylated promoters, Igfbp6 and Foxs1, are upregulated in GDX-induced adrenocortical neoplasms. These new genetic and epigenetic markers may prove useful for studies of steroidogenic cell development and for diagnostic testing. |
|||||
|---|---|---|---|---|---|---|
| Keywords | DNA methylation ; epigenetics ; females ; ferrets ; gene expression regulation ; genes ; gonads ; immunohistochemistry ; in situ hybridization ; males ; messenger RNA ; mice ; microarray technology ; neoplasms ; ovariectomy ; reverse transcriptase polymerase chain reaction ; somatic cells ; transcriptomics | |||||
| Language | English | |||||
| Dates of publication | 2015-0105 | |||||
| Size | p. 122-130. | |||||
| Publishing place | Elsevier Ireland Ltd | |||||
| Document type | Article | |||||
| ZDB-ID | 187438-x | |||||
| ISSN | 1872-8057 ; 0303-7207 | |||||
| ISSN (online) | 1872-8057 | |||||
| ISSN | 0303-7207 | |||||
| DOI | 10.1016/j.mce.2014.09.029 | |||||
| Shelf mark |
|
|||||
| Database | NAL-Catalogue (AGRICOLA) |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 1127: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.