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  1. Article ; Online: Structure-activity studies of Streptococcus pyogenes enzyme SpyCEP reveal high affinity for CXCL8 in the SpyCEP C-terminal.

    Pearson, Max / Haslam, Carl / Fosberry, Andrew / Jones, Emma J / Reglinski, Mark / Reeves, Lucy / Edwards, Robert J / Lawrenson, Richard Ashley / Brown, Jonathan C / Mossakowska, Danuta / Pease, James Edward / Sriskandan, Shiranee

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 19052

    Abstract: The Streptococcus pyogenes cell envelope protease (SpyCEP) is vital to streptococcal pathogenesis and disease progression. Despite its strong association with invasive disease, little is known about enzymatic function beyond the ... ...

    Abstract The Streptococcus pyogenes cell envelope protease (SpyCEP) is vital to streptococcal pathogenesis and disease progression. Despite its strong association with invasive disease, little is known about enzymatic function beyond the ELR
    MeSH term(s) Streptococcus pyogenes/metabolism ; Peptide Hydrolases/metabolism ; Protein Domains
    Chemical Substances Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2023-11-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-46036-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Emerging Invasive Group A Streptococcus M1

    Zhi, Xiangyun / Li, Ho Kwong / Li, Hanqi / Loboda, Zuzanna / Charles, Samson / Vieira, Ana / Huse, Kristin / Jauneikaite, Elita / Reeves, Lucy / Mok, Kai Yi / Coelho, Juliana / Lamagni, Theresa / Sriskandan, Shiranee

    Emerging infectious diseases

    2023  Volume 29, Issue 5, Page(s) 1007–1010

    Abstract: Increasing reports of invasive Streptococcus pyogenes infections mandate surveillance for toxigenic lineage ... ...

    Abstract Increasing reports of invasive Streptococcus pyogenes infections mandate surveillance for toxigenic lineage M1
    MeSH term(s) Humans ; Streptococcus pyogenes/genetics ; Scarlet Fever/epidemiology ; Alleles ; England/epidemiology ; Streptococcal Infections/diagnosis ; Streptococcal Infections/epidemiology ; Polymerase Chain Reaction ; Antigens, Bacterial/genetics ; Bacterial Outer Membrane Proteins/genetics
    Chemical Substances Antigens, Bacterial ; Bacterial Outer Membrane Proteins
    Language English
    Publishing date 2023-04-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2905.221887
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4778: Show issues Location:
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  3. Article ; Online: The protease associated (PA) domain in ScpA from Streptococcus pyogenes plays a role in substrate recruitment.

    McKenna, Sophie / Aylward, Frances / Miliara, Xeni / Lau, Rikin J / Huemer, Camilla Berg / Giblin, Sean P / Huse, Kristin K / Liang, Mingyang / Reeves, Lucy / Pearson, Max / Xu, Yingqi / Rouse, Sarah L / Pease, James E / Sriskandan, Shiranee / Kagawa, Todd F / Cooney, Jakki / Matthews, Stephen

    Biochimica et biophysica acta. Proteins and proteomics

    2023  Volume 1871, Issue 6, Page(s) 140946

    Abstract: Annually, over 18 million disease cases and half a million deaths worldwide are estimated to be caused by Group A Streptococcus. ScpA (or C5a peptidase) is a well characterised member of the cell enveleope protease family, which possess a S8 subtilisin- ... ...

    Abstract Annually, over 18 million disease cases and half a million deaths worldwide are estimated to be caused by Group A Streptococcus. ScpA (or C5a peptidase) is a well characterised member of the cell enveleope protease family, which possess a S8 subtilisin-like catalytic domain and a shared multi-domain architecture. ScpA cleaves complement factors C5a and C3a, impairing the function of these critical anaphylatoxins and disrupts complement-mediated innate immunity. Although the high resolution structure of ScpA is known, the details of how it recognises its substrate are only just emerging. Previous studies have identified a distant exosite on the 2nd fibronectin domain that plays an important role in recruitment via an interaction with the substrate core. Here, using a combination of solution NMR spectroscopy, mutagenesis with functional assays and computational approaches we identify a second exosite within the protease-associated (PA) domain. We propose a model in which the PA domain assists optimal delivery of the substrate's C terminus to the active site for cleavage.
    MeSH term(s) Peptide Hydrolases ; Streptococcus pyogenes ; Immunity, Innate
    Chemical Substances Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2023-08-09
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2918798-9
    ISSN 1878-1454 ; 1570-9639
    ISSN (online) 1878-1454
    ISSN 1570-9639
    DOI 10.1016/j.bbapap.2023.140946
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 7161: Show issues Location:
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  4. Article ; Online: The protease associated (PA) domain in ScpA from Streptococcus pyogenes plays a role in substrate recruitment

    McKenna, Sophia / Aylward, Frances / Miliara, Xeni / Lau, Rikin J. / Huemer, Camilla Berg / Giblin, Sean P. / Huse, Kristin K. / Liang, Mingyang / Reeves, Lucy / Pearson, Max / Xu, Yingqi / Rouse, Sarah L. / Pease, James E. / Sriskandan, Shiranee / Kagawa, Todd F. / Cooney, Jakki / Matthews, Stephen

    BBA - Proteins and Proteomics. 2023 Aug. 09, p.140946-

    2023  , Page(s) 140946–

    Abstract: Annually, over 18 million disease cases and half a million deaths worldwide are estimated to be caused by Group A Streptococcus. ScpA (or C5a peptidase) is a well characterised member of the cell enveleope protease family, which possess a S8 subtilisin- ... ...

    Abstract Annually, over 18 million disease cases and half a million deaths worldwide are estimated to be caused by Group A Streptococcus. ScpA (or C5a peptidase) is a well characterised member of the cell enveleope protease family, which possess a S8 subtilisin-like catalytic domain and a shared multi-domain architecture. ScpA cleaves complement factors C5a and C3a, impairing the function of these critical anaphylatoxins and disrupts complement-mediated innate immunity. Although the of high resolution of structure ScpA is known, the details of how it recognises its substrate are only just emerging. Previous studies have identified a distant exosite on the 2nd fibronectin domain that plays an important role in recruitment via an interaction with the substrate core. Here, using a combination of solution NMR spectroscopy, mutagenesis with functional assays and computational approaches we identify a second exosite within the protease-associated (PA) domain. We propose a model in which the PA domain assists optimal delivery of the substrate's C terminus to the active site for cleavage.
    Keywords Streptococcus pyogenes ; active sites ; complement ; fibronectins ; innate immunity ; models ; mutagenesis ; nuclear magnetic resonance spectroscopy ; proteinases ; proteomics ; Bacterial cell envelope proteases ; ScpA ; c5a and c3a ; Solution NMR ; Group A Streptococcus
    Language English
    Dates of publication 2023-0809
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 2918798-9
    ISSN 1878-1454 ; 1570-9639
    ISSN (online) 1878-1454
    ISSN 1570-9639
    DOI 10.1016/j.bbapap.2023.140946
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 7161: Show issues Location:
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  5. Article ; Online: Rapid expansion and international spread of M1

    Vieira, Ana / Wan, Yu / Ryan, Yan / Li, Ho Kwong / Guy, Rebecca L / Papangeli, Maria / Huse, Kristin K / Reeves, Lucy C / Soo, Valerie W C / Daniel, Roger / Harley, Alessandra / Broughton, Karen / Dhami, Chenchal / Ganner, Mark / Ganner, Marjorie A / Mumin, Zaynab / Razaei, Maryam / Rundberg, Emma / Mammadov, Rufat /
    Mills, Ewurabena A / Sgro, Vincenzo / Mok, Kai Yi / Didelot, Xavier / Croucher, Nicholas J / Jauneikaite, Elita / Lamagni, Theresa / Brown, Colin S / Coelho, Juliana / Sriskandan, Shiranee

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 3916

    Abstract: The UK observed a marked increase in scarlet fever and invasive group A streptococcal infection in 2022 with severe outcomes in children and similar trends worldwide. Here we report lineage ... ...

    Abstract The UK observed a marked increase in scarlet fever and invasive group A streptococcal infection in 2022 with severe outcomes in children and similar trends worldwide. Here we report lineage M1
    MeSH term(s) Streptococcus pyogenes/genetics ; Streptococcus pyogenes/pathogenicity ; Streptococcus pyogenes/isolation & purification ; United Kingdom/epidemiology ; Humans ; Streptococcal Infections/epidemiology ; Streptococcal Infections/microbiology ; Phylogeny ; COVID-19/epidemiology ; Pandemics ; Scarlet Fever/epidemiology ; Scarlet Fever/microbiology ; Mutation ; Repressor Proteins/genetics ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Genome, Bacterial ; Europe/epidemiology ; Bacterial Proteins
    Chemical Substances CsrR protein, Streptococcus pyogenes ; Repressor Proteins ; Bacterial Proteins
    Language English
    Publishing date 2024-05-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-47929-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Transmission of SARS-CoV-2 by children to contacts in schools and households: a prospective cohort and environmental sampling study in London.

    Cordery, Rebecca / Reeves, Lucy / Zhou, Jie / Rowan, Aileen / Watber, Patricia / Rosadas, Carolina / Crone, Michael / Storch, Marko / Freemont, Paul / Mosscrop, Lucy / Cowley, Alice / Zelent, Gina / Bisset, Kate / Le Blond, Holly / Regmi, Sadie / Buckingham, Christian / Junaideen, Ramlah / Abdulla, Nadia / Eliahoo, Joseph /
    Mindlin, Miranda / Lamagni, Theresa / Barclay, Wendy / Taylor, Graham P / Sriskandan, Shiranee

    The Lancet. Microbe

    2022  Volume 3, Issue 11, Page(s) e814–e823

    Abstract: Background: Assessing transmission of SARS-CoV-2 by children in schools is of crucial importance to inform public health action. We assessed frequency of acquisition of SARS-CoV-2 by contacts of pupils with COVID-19 in schools and households, and ... ...

    Abstract Background: Assessing transmission of SARS-CoV-2 by children in schools is of crucial importance to inform public health action. We assessed frequency of acquisition of SARS-CoV-2 by contacts of pupils with COVID-19 in schools and households, and quantified SARS-CoV-2 shedding into air and onto fomites in both settings.
    Methods: We did a prospective cohort and environmental sampling study in London, UK in eight schools. Schools reporting new cases of SARS-CoV-2 infection to local health protection teams were invited to take part if a child index case had been attending school in the 48 h before a positive SARS-CoV-2 PCR test. At the time of the study, PCR testing was available to symptomatic individuals only. Children aged 2-14 years (extended to <18 years in November, 2020) with a new nose or throat swab SARS-CoV-2 positive PCR from an accredited laboratory were included. Incidents involving exposure to at least one index pupil with COVID-19 were identified (the prevailing variants were original, α, and δ). Weekly PCR testing for SARS-CoV-2 was done on immediate classroom contacts (the so-called bubble), non-bubble school contacts, and household contacts of index pupils. Testing was supported by genome sequencing and on-surface and air samples from school and home environments.
    Findings: Between October, 2020, and July, 2021 from the eight schools included, secondary transmission of SARS-CoV-2 was not detected in 28 bubble contacts, representing ten bubble classes (participation rate 8·8% [IQR 4·6-15·3]). Across eight non-bubble classes, 3 (2%) of 62 pupils tested positive, but these were unrelated to the original index case (participation rate 22·5% [9·7-32·3]). All three were asymptomatic and tested positive in one setting on the same day. In contrast, secondary transmission to previously negative household contacts from infected index pupils was found in six (17%) of 35 household contacts rising to 13 (28%) of 47 household contacts when considering all potential infections in household contacts. Environmental contamination with SARS-CoV-2 was rare in schools: fomite SARS-CoV-2 was identified in four (2%) of 189 samples in bubble classrooms, two (2%) of 127 samples in non-bubble classrooms, and five (4%) of 130 samples in washrooms. This contrasted with fomites in households, where SARS-CoV-2 was identified in 60 (24%) of 248 bedroom samples, 66 (27%) of 241 communal room samples, and 21 (11%) 188 bathroom samples. Air sampling identified SARS-CoV-2 RNA in just one (2%) of 68 of school air samples, compared with 21 (25%) of 85 air samples taken in homes.
    Interpretation: There was no evidence of large-scale SARS-CoV-2 transmission in schools with precautions in place. Low levels of environmental contamination in schools are consistent with low transmission frequency and suggest adequate cleaning and ventilation in schools during the period of study. The high frequency of secondary transmission in households associated with evident viral shedding throughout the home suggests a need to improve advice to households with infection in children to prevent onward community spread. The data suggest that SARS-CoV-2 transmission from children in any setting is very likely to occur when precautions are reduced.
    Funding: UK Research and Innovation and UK Department of Health and Social Care, National Institute for Health and Care Research.
    MeSH term(s) Child ; Humans ; SARS-CoV-2 ; COVID-19/epidemiology ; Sampling Studies ; Prospective Studies ; London/epidemiology ; RNA, Viral ; Schools
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2022-08-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-5247
    ISSN (online) 2666-5247
    DOI 10.1016/S2666-5247(22)00124-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Transmission of SARS-CoV-2 by children attending school. Interim report on an observational, longitudinal sampling study of infected children, contacts, and the environment

    Cordery, Rebecca / Reeves, Lucy / Zhou, Jie / Rowan, Aileen G. / Watber, Patricia / Rosadas, Carolina / Crone, Michael Andrew / Freemont, Paul / Mosscrop, Lucy / Cowley, Alice / Zelent, Gina / Bisset, Kate / LeBlond, Holly / Regmi, Sadie / Mindlin, Miranda / Lamagni, Theresa / Barclay, Wendy / Taylor, Graham P / Sriskandan, Shiranee

    medRxiv

    Abstract: Background. Transmission of SARS-CoV-2 by children and young people in school settings has not been directly evaluated, nor the main mechanisms of transmission identified. The study set out to undertake sequential longitudinal sampling of infected ... ...

    Abstract Background. Transmission of SARS-CoV-2 by children and young people in school settings has not been directly evaluated, nor the main mechanisms of transmission identified. The study set out to undertake sequential longitudinal sampling of infected children, their contacts, and the environment. Methods. Cases of COVID-19 were identified through statutory notification and matched to schools reporting cases. Cases of COVID-19 and their contacts from school and home were longitudinally sampled and tested for SARS-CoV-2. Surfaces and air in the home and school environment were also subject to longitudinal sampling and testing. Results Onward transmission of virus to immediate classroom members who participated in the study was not detected. Evidence of more widespread transmission among children remaining in school was not identified with the exception of one unexpected cluster of three asymptomatic cases in one school. Children infected with SARS-CoV-2 in this study shed viral RNA for up to 10 days from symptom onset, with levels peaking at 5-8 days. Viral RNA was identified in the environment around children who were actively shedding virus in the home, but limited contamination was identified in schools. Variant of Concern B1.1.7 was identified in later cases studied. Summary After 3 months, this small study has not found evidence to suggest COVID-19 is commonly transmitted by children within schools. A minority of infections may be subject to stochastic events that can lead to transmission. Further prospective and retrospective studies are required to identify factors associated with such events .
    Keywords covid19
    Language English
    Publishing date 2021-03-09
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2021.03.08.21252839
    Database COVID19

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