Article ; Online: Mild dehydration effects on the murine kidney single-nucleus transcriptome and chromatin accessibility.
American journal of physiology. Renal physiology
2023 Volume 325, Issue 6, Page(s) F717–F732
Abstract: Daily, we may experience mild dehydration with a rise in plasma osmolality that triggers the release of vasopressin. Although the effect of dehydration is well characterized in collecting duct principal cells (CDPCs), we hypothesized that mild ... ...
| Abstract | Daily, we may experience mild dehydration with a rise in plasma osmolality that triggers the release of vasopressin. Although the effect of dehydration is well characterized in collecting duct principal cells (CDPCs), we hypothesized that mild dehydration (<12 h) results in many kidney cell-specific changes in transcriptomes and chromatin accessibility. Single-nucleus (sn) multiome (RNA-assay for transposase-accessible chromatin) sequencing and bulk RNA sequencing of kidneys from male and female mice that were mildly water deprived or not were compared. Water-deprived mice had a significant increase in plasma osmolality. sn-multiome-seq resulted in 19,837 nuclei that were annotated into 33 clusters. In CDPCs, aquaporin 2 ( |
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| MeSH term(s) | Mice ; Animals ; Male ; Female ; Dehydration/metabolism ; Transcriptome ; Chromatin/genetics ; Chromatin/metabolism ; Aquaporin 2/genetics ; Aquaporin 2/metabolism ; Kidney/metabolism ; Water/metabolism | |||||
| Chemical Substances | Chromatin ; Aquaporin 2 ; Water (059QF0KO0R) | |||||
| Language | English | |||||
| Publishing date | 2023-09-28 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, N.I.H., Extramural | |||||
| ZDB-ID | 603837-2 | |||||
| ISSN | 1522-1466 ; 0363-6127 | |||||
| ISSN (online) | 1522-1466 | |||||
| ISSN | 0363-6127 | |||||
| DOI | 10.1152/ajprenal.00161.2023 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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