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  1. Article ; Online: Leucine-Rich Glioma-Inactivated 1 (LGI1) Protein Stimulates Proliferation and IL-10 Production in Peripheral Blood Mononuclear Cells of Patients with LGI1 Antibody-Mediated Autoimmune Encephalitis In Vitro.

    Goihl, Alexander / Reinhold, Dirk / Reinhold, Annegret / Schraven, Burkhart / Körtvelyessy, Peter

    International journal of molecular sciences

    2024  Volume 25, Issue 5

    Abstract: Limbic encephalitis (LE) due to anti-leucine-rich glioma-inactivated 1 (LGI1) antibodies is an autoimmune disease characterized by distinct clinical features unique to LGI1 LE, such as faciobrachial dystonic seizures. However, it is unclear whether an ... ...

    Abstract Limbic encephalitis (LE) due to anti-leucine-rich glioma-inactivated 1 (LGI1) antibodies is an autoimmune disease characterized by distinct clinical features unique to LGI1 LE, such as faciobrachial dystonic seizures. However, it is unclear whether an additional disease-related LGI1 antigen-specific T cell response is involved in the pathogenesis of this disease. To address this question, we studied the effect of recombinant LGI1 on the proliferation and effector-specific cytokine production (IFN-γ, IL-5, IL-10, and IL-17) of peripheral blood mononuclear cells (PBMCs) from patients with LGI1 LE and healthy controls. We observed that recombinant LGI1 stimulated the proliferation of PBMCs from patients with LGI1 LE, but not from healthy controls. Cytokine measurement of cell culture supernatants from PBMCs incubated with recombinant LGI1 revealed a highly significant increase in IL-10 release in PBMCs from patients with LGI1 LE in comparison with healthy controls. These results suggest that LGI1-mediated stimulation of PBMCs from patients with LGI1 LE leads to the establishment of an IL-10-dominated immunosuppressive cytokine milieu, which may inhibit Th1 differentiation and support B cell proliferation, IgG production, and IgG subclass switching.
    MeSH term(s) Humans ; Leucine ; Intracellular Signaling Peptides and Proteins ; Leukocytes, Mononuclear/pathology ; Interleukin-10 ; Limbic Encephalitis ; Immunoglobulin G ; Autoimmune Diseases of the Nervous System ; Glioma
    Chemical Substances Leucine (GMW67QNF9C) ; Intracellular Signaling Peptides and Proteins ; Interleukin-10 (130068-27-8) ; Immunoglobulin G ; LGI1 protein, human
    Language English
    Publishing date 2024-02-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25052581
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  2. Article: Why a pinch of zinc in liver disease matters.

    Grüngreiff, Kurt / Reinhold, Dirk / Maret, Wolfgang

    Annals of hepatology

    2023  Volume 29, Issue 1, Page(s) 101152

    MeSH term(s) Humans ; Zinc/therapeutic use ; Liver Diseases/diagnosis
    Chemical Substances Zinc (J41CSQ7QDS)
    Language English
    Publishing date 2023-09-11
    Publishing country Mexico
    Document type Journal Article
    ZDB-ID 2188733-0
    ISSN 1665-2681
    ISSN 1665-2681
    DOI 10.1016/j.aohep.2023.101152
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6221: Show issues Location:
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  3. Article ; Online: Allgemeine Kenntnisse zu häufig angewandten Testverfahren in der rheumatologisch-immunologischen Diagnostik und Forschung.

    Reinhold, Dirk / Reinhold, Annegret / Schraven, Burkhart / Feist, Eugen

    Zeitschrift fur Rheumatologie

    2023  Volume 82, Issue 4, Page(s) 278–284

    Abstract: Knowledge of test procedures is essential for an optimal approach in rheumatological and immunological diagnostics as well as for a correct interpretation of the findings. In practice, they are a basis for the independent provision of diagnostic ... ...

    Title translation General knowledge of frequently applied test procedures in rheumatological and immunological diagnostics and research.
    Abstract Knowledge of test procedures is essential for an optimal approach in rheumatological and immunological diagnostics as well as for a correct interpretation of the findings. In practice, they are a basis for the independent provision of diagnostic laboratory services. For scientific questions they have become indispensable tools in many areas. This article gives an overview on the most frequently used and important test methods in a comprehensive form. The advantages and performance of the different methods are addressed and the limitations and possible sources or error are discussed. Quality control increasingly plays a decisive role in the diagnostic and scientific practice, with the legal regulations applying to all test procedures in laboratory diagnostics. For the discipline of rheumatology, the rheumatological and immunological diagnostics are of particular importance as the majority of the known disease-specific markers are detectable by means of these procedures. At the same time, immunological laboratory diagnostics are a highly interesting field of activity which are expected to have a strong impact on the future developments in rheumatology.
    MeSH term(s) Humans ; Rheumatology ; Immunologic Tests ; Clinical Laboratory Techniques ; Forecasting ; Rheumatic Diseases/diagnosis
    Language German
    Publishing date 2023-03-03
    Publishing country Germany
    Document type English Abstract ; Journal Article ; Review
    ZDB-ID 124985-x
    ISSN 1435-1250 ; 0340-1855 ; 0301-6382
    ISSN (online) 1435-1250
    ISSN 0340-1855 ; 0301-6382
    DOI 10.1007/s00393-023-01335-4
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    Uh III Zs.128: Show issues Location:
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  4. Book ; Thesis: Untersuchungen zur Rolle der enzymatischen Aktivität der Dipetidylpeptidase IV (DP IV/CD26) bei der T-Zellaktivierung in vitro und in vivo

    Reinhold, Dirk

    1999  

    Author's details vorgelegt von Dirk Reinhold
    Language German ; English
    Size 213 Bl. : Ill., graph. Darst.
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Magdeburg, Univ., Habil.-Schr., 2000
    Note Beitr. teilw. dt., teilw. engl. - Enthält 16 Sonderabdr. aus verschiedenen Zeitschr.
    HBZ-ID HT012891934
    Database Catalogue ZB MED Medicine, Health

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  5. Article ; Online: Zinc aspartate induces proliferation of resting and antigen-stimulated human PBMC under high-density cell culture condition.

    Guttek, Karina / Reinhold, Annegret / Grüngreiff, Kurt / Schraven, Burkhart / Reinhold, Dirk

    Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)

    2023  Volume 77, Page(s) 127152

    Abstract: Background: Zinc, one of the most important essential trace elements in the human body, regulates a wide range of cellular functions of immune cells, such as proliferation, differentiation and survival. Zinc deficiency affects both the innate and ... ...

    Abstract Background: Zinc, one of the most important essential trace elements in the human body, regulates a wide range of cellular functions of immune cells, such as proliferation, differentiation and survival. Zinc deficiency affects both the innate and adaptive immune system. Zinc supplementation was discussed as possible therapy for infectious diseases and T cell-mediated autoimmune diseases. However, the influence of commercial zinc preparations on proliferation and cytokine production of resting and antigen-stimulated peripheral blood mononuclear cells (PBMC) has not yet been completely investigated.
    Methods: Here, we examined whether zinc aspartate (Unizink®), an approved drug to treat zinc deficiency in patients, induces proliferation, cytokine production, and induction of apoptosis/caspase 3/7 activity of resting PBMC under high-density cell culture condition. In addition, we performed antigen-specific proliferation experiments, where PBMCs of healthy donors vaccinated against Influenza A (H1N1) and/or SARS-CoV-2 were stimulated with Influenza A (H1N1) peptides or SARS-CoV-2 peptides as well as the Mixed Lymphocyte Culture (MLC) in the presence of increasing concentrations of zinc aspartate.
    Results: We observed a dose-dependent enhancement of proliferation and induction of cytokine production (IFN-γ, IL-5, GM-CSF and CXCL10) of resting PBMC in presence of zinc aspartate. The number of cells with active caspase 3/7 and, consecutively, the amount of cells undergoing apoptosis steadily decreased in presence of zinc aspartate. Moreover, zinc aspartate was capable of stimulating antigen-specific PBMC proliferation using MLC or influenza A (H1N1) and SARS-CoV-2 peptides in both a dose-dependent and a donor-specific manner. In the absence of zinc aspartate, we clearly could discriminate two groups of responders: low and high responders to antigenic stimulation. The addition of increasing concentration of zinc aspartate significantly stimulated the proliferation of PBMC from low responders, but not from high responders.
    Conclusion: Taken together, our results suggest that zinc aspartate induces the proliferation of resting and antigen-stimulated PBMCs under high-density cell culture conditions. Thus, zinc might represent a supportive treatment in patients suffering from infectious diseases.
    MeSH term(s) Humans ; Leukocytes, Mononuclear ; Caspase 3 ; Influenza A Virus, H1N1 Subtype ; Influenza, Human ; COVID-19 ; SARS-CoV-2 ; Cell Culture Techniques ; Cell Proliferation ; Zinc/pharmacology ; Cytokines
    Chemical Substances zinc aspartate (4OC7QTI23H) ; Caspase 3 (EC 3.4.22.-) ; Zinc (J41CSQ7QDS) ; Cytokines
    Language English
    Publishing date 2023-03-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1236267-0
    ISSN 1878-3252 ; 1611-602X ; 0946-672X
    ISSN (online) 1878-3252 ; 1611-602X
    ISSN 0946-672X
    DOI 10.1016/j.jtemb.2023.127152
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2279: Show issues Location:
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  6. Book ; Online ; Thesis: Untersuchungen zur Wirkung von Adefovir-Dipivoxil und Pitavastatin auf die Funktion humaner T-Zellen

    Voss, Linda [Verfasser] / Reinhold, Dirk [Gutachter]

    2022  

    Author's details Linda Voss ; Gutachter: Dirk Reinhold
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language German
    Publisher Universitätsbibliothek Otto-von-Guericke-Universität
    Publishing place Magdeburg
    Document type Book ; Online ; Thesis
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  7. Article ; Online: The differential roles of zinc in immune responses and their potential implications in antiviral immunity against SARS-CoV-2.

    Reinhold, Dirk / Brocke, Stefan

    Clinical nutrition (Edinburgh, Scotland)

    2020  Volume 40, Issue 2, Page(s) 652

    MeSH term(s) COVID-19/immunology ; Humans ; Immunity, Innate ; Zinc/deficiency ; Zinc/immunology ; Zinc/therapeutic use
    Chemical Substances Zinc (J41CSQ7QDS)
    Language English
    Publishing date 2020-12-10
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 604812-2
    ISSN 1532-1983 ; 0261-5614
    ISSN (online) 1532-1983
    ISSN 0261-5614
    DOI 10.1016/j.clnu.2020.12.005
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    Zs.A 1774: Show issues Location:
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  8. Book ; Thesis: Untersuchungen zum Einfluß der Dipeptidylpeptidase IV und der Aminopeptidase N auf die Proliferation von Immunzellen

    Reinhold, Dirk

    1992  

    Author's details vorgelegt von Dirk Reinhold
    Size 92 Bl. : graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Magdeburg, Med. Akad., Diss., 1992
    HBZ-ID HT004423910
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    93 E 511 order for loan Magazin

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  9. Article ; Online: Patterns of allergic sensitization in adults with severe asthma: the ATLAS non-interventional study.

    Lücke, Eva / Schraven, Burkhart / Borucki, Katrin / Lux, Anke / Reinhold, Dirk / Wu, Qingyu / Schreiber, Jens

    The Journal of asthma : official journal of the Association for the Care of Asthma

    2023  Volume 60, Issue 11, Page(s) 2021–2029

    Abstract: Objectives: Severe asthma is heterogeneous, with childhood-onset asthma believed more likely to be allergic, whereas adult-onset asthma is considered typically non-allergic. However, the allergic diagnosis is typically by exclusion: if patients do not ... ...

    Abstract Objectives: Severe asthma is heterogeneous, with childhood-onset asthma believed more likely to be allergic, whereas adult-onset asthma is considered typically non-allergic. However, the allergic diagnosis is typically by exclusion: if patients do not react to an allergen panel, which is not standardized and often limited to few allergens, they are considered non-allergic. The overall aim of the ATLAS study was to characterize the sensitization to allergens in severe asthma (independent of phenotype).
    Methods: Single-visit, cross-sectional, non-interventional study in adults with severe asthma. Analyses were conducted for total and specific immunoglobulin E against 53 allergens, overall and in subgroups, including age at asthma onset (<20 [childhood-onset] and >40 years of age).
    Results: Among 1010 recruited patients, 28.4% reported childhood-onset asthma and 33.6% onset >40 years of age. After excluding patients receiving omalizumab/anti-IL5 therapy, 27.6% were not sensitized to any tested allergens, whereas 19.1% were sensitized to >10 allergens. All allergens triggered sensitization in some patients. Baseline characteristics in the two onset subgroups were similar; 23.2% with childhood-onset asthma were not sensitized to any allergen, compared to 32.0% with onset >40 years of age.
    Conclusion: When a broad panel of allergens is used for sensitization testing, as many as three quarters of patients with severe asthma display sensitivity to at least one allergen, with substantial overlaps in all characteristics between the two age-at-onset subgroups. All of the tested allergens triggered a response in at least some patients, emphasizing the importance of including a broad range of allergens in any testing panel.
    Language English
    Publishing date 2023-05-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 603816-5
    ISSN 1532-4303 ; 0277-0903
    ISSN (online) 1532-4303
    ISSN 0277-0903
    DOI 10.1080/02770903.2023.2213327
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  10. Book ; Online ; Thesis: Identifikation neuer Antigene für die Diagnose von chronisch entzündlichen Darmerkrankungen

    Deutschmann, Claudia Verfasser] / [Schierack, Peter [Gutachter] / Reinhold, Dirk [Gutachter]

    2022  

    Author's details Claudia Deutschmann ; Gutachter: Peter Schierack, Dirk Reinhold
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher BTU Cottbus - Senftenberg
    Publishing place Cottbus
    Document type Book ; Online ; Thesis
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