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  1. Article ; Online: Extracellular Vesicles and Particles Modulate Proton Secretion in a Model of Human Parietal Cells.

    Mistlberger-Reiner, Agnes / Sterneder, Sonja / Reipert, Siegfried / Wolske, Sara / Somoza, Veronika

    ACS omega

    2023  Volume 8, Issue 2, Page(s) 2213–2226

    Abstract: The secretion of extracellular vesicles and particles (EVPs) is an important mechanism of cellular communication. In this work, we demonstrate a functional role of EVPs in mechanisms regulating gastric acid secretion. HGT-1 cells were used as a model ... ...

    Abstract The secretion of extracellular vesicles and particles (EVPs) is an important mechanism of cellular communication. In this work, we demonstrate a functional role of EVPs in mechanisms regulating gastric acid secretion. HGT-1 cells were used as a model system to assess proton secretion. First, in order to prove EVP secretion by HGT-1 cells, EVPs were isolated by size exclusion chromatography and characterized by nanoparticle tracking analysis, Western blot, and cryo transmission electron microscopy. For examination of the potential role of EVPs in proton secretion, HGT-1 cells were treated with pharmacological EV-inhibitors, resulting in a reduction of histamine-induced proton secretion. To demonstrate the functional role of EVPs in the mechanism of proton secretion, EVP-conditioned supernatant was collected after stimulation of HGT-1 cells with histamine, fractionated, and subjected to an activity screening. The results revealed constituents of the HGT-1-derived secretome with an MW of >100 kDa (including EVPs) to modulate proton secretion, while smaller constituents had no effect. Finally, a dose-dependent modulatory effect on proton secretion of HGT-1 cells was demonstrated by isolated HGT-1-derived EVPs. Hence, this study presents first results on the potential function of EVPs as a previously undiscovered mechanism of regulation of gastric acid secretion by parietal cells.
    Language English
    Publishing date 2023-01-05
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.2c06442
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A giant virus infecting the amoeboflagellate Naegleria.

    Arthofer, Patrick / Panhölzl, Florian / Delafont, Vincent / Hay, Alban / Reipert, Siegfried / Cyran, Norbert / Wienkoop, Stefanie / Willemsen, Anouk / Sifaoui, Ines / Arberas-Jiménez, Iñigo / Schulz, Frederik / Lorenzo-Morales, Jacob / Horn, Matthias

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 3307

    Abstract: Giant viruses (Nucleocytoviricota) are significant lethality agents of various eukaryotic hosts. Although metagenomics indicates their ubiquitous distribution, available giant virus isolates are restricted to a very small number of protist and algal ... ...

    Abstract Giant viruses (Nucleocytoviricota) are significant lethality agents of various eukaryotic hosts. Although metagenomics indicates their ubiquitous distribution, available giant virus isolates are restricted to a very small number of protist and algal hosts. Here we report on the first viral isolate that replicates in the amoeboflagellate Naegleria. This genus comprises the notorious human pathogen Naegleria fowleri, the causative agent of the rare but fatal primary amoebic meningoencephalitis. We have elucidated the structure and infection cycle of this giant virus, Catovirus naegleriensis (a.k.a. Naegleriavirus, NiV), and show its unique adaptations to its Naegleria host using fluorescence in situ hybridization, electron microscopy, genomics, and proteomics. Naegleriavirus is only the fourth isolate of the highly diverse subfamily Klosneuvirinae, and like its relatives the NiV genome contains a large number of translation genes, but lacks transfer RNAs (tRNAs). NiV has acquired genes from its Naegleria host, which code for heat shock proteins and apoptosis inhibiting factors, presumably for host interactions. Notably, NiV infection was lethal to all Naegleria species tested, including the human pathogen N. fowleri. This study expands our experimental framework for investigating giant viruses and may help to better understand the basic biology of the human pathogen N. fowleri.
    MeSH term(s) Genome, Viral/genetics ; Giant Viruses/genetics ; Giant Viruses/classification ; Giant Viruses/ultrastructure ; Giant Viruses/isolation & purification ; Giant Viruses/physiology ; Naegleria/genetics ; Naegleria/virology ; Naegleria fowleri/genetics ; Naegleria fowleri/isolation & purification ; Phylogeny ; Humans
    Language English
    Publishing date 2024-04-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-47308-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Microtubule-tethering junctions are fine structural distinct entities that deserve attention.

    Reipert, Siegfried

    Traffic (Copenhagen, Denmark)

    2007  Volume 8, Issue 12, Page(s) 1706–1707

    Abstract: Microtubule tethering at cell junctions, as studied by light microscopy (Ligon and Holzbaur, Traffic 2007;8:808-819), exist as easily recognizable ultrastructural entities that are distinct from adherence junctions. Here, we encourage systematic analysis ...

    Abstract Microtubule tethering at cell junctions, as studied by light microscopy (Ligon and Holzbaur, Traffic 2007;8:808-819), exist as easily recognizable ultrastructural entities that are distinct from adherence junctions. Here, we encourage systematic analysis of cortical microtubule capture by electron microscopy.
    MeSH term(s) Animals ; Cell Adhesion ; Cell Line ; Kinesins/chemistry ; Microscopy, Electron/methods ; Microscopy, Fluorescence/methods ; Microtubules/chemistry ; Microtubules/metabolism ; Microtubules/ultrastructure ; Microwaves ; Potoroidae ; Protein Conformation ; Tubulin/chemistry
    Chemical Substances Tubulin ; Kinesins (EC 3.6.4.4)
    Language English
    Publishing date 2007-10-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1483852-7
    ISSN 1600-0854 ; 1398-9219
    ISSN (online) 1600-0854
    ISSN 1398-9219
    DOI 10.1111/j.1600-0854.2007.00656.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5634: Show issues Location:
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  4. Article: Freeze Substitution Accelerated via Agitation: New Prospects for Ultrastructural Studies of Lichen Symbionts and Their Extracellular Matrix.

    Reipert, Siegfried / Gruber, Daniela / Cyran, Norbert / Schmidt, Brigitte / de la Torre Noetzel, Rosa / Sancho, Leopoldo G / Goga, Michal / Bačkor, Martin / Schmidt, Katy

    Plants (Basel, Switzerland)

    2023  Volume 12, Issue 23

    Abstract: 1) Background: Lichens, as an important part of the terrestrial ecosystem, attract the attention of various research disciplines. To elucidate their ultrastructure, transmission electron microscopy of resin-embedded samples is indispensable. Since most ... ...

    Abstract (1) Background: Lichens, as an important part of the terrestrial ecosystem, attract the attention of various research disciplines. To elucidate their ultrastructure, transmission electron microscopy of resin-embedded samples is indispensable. Since most observations of lichen samples are generated via chemical fixation and processing at room temperature, they lack the rapid immobilization of live processes and are prone to preparation artefacts. To improve their preservation, cryoprocessing was tested in the past, but never widely implemented, not least because of an extremely lengthy protocol. (2) Methods: Here, we introduce an accelerated automated freeze substitution protocol with continuous agitation. Using the example of three lichen species, we demonstrate the preservation of the native state of algal photobionts and mycobionts in association with their extracellular matrix. (3) Results: We bring to attention the extent and the structural variability of the hyphae, the extracellular matrix and numerous crystallized metabolites. Our findings will encourage studies on transformation processes related to the compartmentation of lichen thalli. They include cryopreserved aspects of algal photobionts and observations of putative physiological relevance, such as the arrangement of numerous mitochondria within chloroplast pockets. (4) Conclusions: In summary, we present accelerated freeze substitution as a very useful tool for systematic studies of lichen ultrastructures.
    Language English
    Publishing date 2023-11-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants12234039
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Microtubule-Tethering Junctions are Fine Structural Distinct Entities that Deserve Attention

    Reipert, Siegfried

    Traffic. 2007 Dec., v. 8, no. 12

    2007  

    Abstract: Microtubule tethering at cell junctions, as studied by light microscopy (Ligon and Holzbaur, Traffic 2007;8:808-819), exist as easily recognizable ultrastructural entities that are distinct from adherence junctions. Here, we encourage systematic analysis ...

    Abstract Microtubule tethering at cell junctions, as studied by light microscopy (Ligon and Holzbaur, Traffic 2007;8:808-819), exist as easily recognizable ultrastructural entities that are distinct from adherence junctions. Here, we encourage systematic analysis of cortical microtubule capture by electron microscopy.
    Keywords electron microscopy ; microtubules ; ultrastructure
    Language English
    Dates of publication 2007-12
    Size p. 1706-1707.
    Publisher Blackwell Publishing Ltd
    Publishing place Oxford, UK
    Document type Article
    ZDB-ID 1483852-7
    ISSN 1600-0854 ; 1398-9219
    ISSN (online) 1600-0854
    ISSN 1398-9219
    DOI 10.1111/j.1600-0854.2007.00656.x
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

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  6. Article: Anaerobic Sulfur Oxidation Underlies Adaptation of a Chemosynthetic Symbiont to Oxic-Anoxic Interfaces.

    Paredes, Gabriela F / Viehboeck, Tobias / Lee, Raymond / Palatinszky, Marton / Mausz, Michaela A / Reipert, Siegfried / Schintlmeister, Arno / Maier, Andreas / Volland, Jean-Marie / Hirschfeld, Claudia / Wagner, Michael / Berry, David / Markert, Stephanie / Bulgheresi, Silvia / König, Lena

    mSystems

    2021  Volume 6, Issue 3, Page(s) e0118620

    Abstract: Chemosynthetic symbioses occur worldwide in marine habitats, but comprehensive physiological studies of chemoautotrophic bacteria thriving on animals are scarce. Stilbonematinae are coated by ... ...

    Abstract Chemosynthetic symbioses occur worldwide in marine habitats, but comprehensive physiological studies of chemoautotrophic bacteria thriving on animals are scarce. Stilbonematinae are coated by thiotrophic
    Language English
    Publishing date 2021-05-26
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5077
    ISSN 2379-5077
    DOI 10.1128/mSystems.01186-20
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  7. Article ; Online: Deficiency of Cathelicidin-related Antimicrobial Peptide Promotes Skin Papillomatosis in Mus musculus Papillomavirus 1-infected Mice.

    Dorfer, Sonja / Strasser, Katharina / Reipert, Siegfried / Fischer, Michael B / Shafti-Keramat, Saeed / Bonelli, Michael / Schröckenfuchs, Georg / Bauer, Wolfgang / Kancz, Stefanie / Müller, Lena / Handisurya, Alessandra

    Acta dermato-venereologica

    2021  Volume 101, Issue 1, Page(s) adv00367

    Abstract: Cathelicidins have been reported to inhibit human papillomavirus infection in vitro; however, nothing is known about their activity in vivo. In this study, experimental skin infection with Mus musculus papillomavirus 1 resulted in robust development of ... ...

    Abstract Cathelicidins have been reported to inhibit human papillomavirus infection in vitro; however, nothing is known about their activity in vivo. In this study, experimental skin infection with Mus musculus papillomavirus 1 resulted in robust development of cutaneous papillomas in cyclosporine A-treated C57BL/6J mice deficient for the murine cathelicidin-related antimicrobial peptide (CRAMP), in contrast to wild-type controls. Analysis of the underlying mechanisms revealed moderate disruption of virion integrity and lack of interference with viral entry and intracellular trafficking by a synthetic CRAMP peptide. Differences in the immune response to Mus musculus papillomavirus 1 infection were observed between CRAMP-deficient and wild-type mice. These included a stronger reduction in CD4+ and CD8+ T-cell numbers in infected skin, and lack of Mus musculus papillomavirus 1-specific neutralizing antibodies in response to cyclosporine A in the absence of endogenous CRAMP. CRAMP has modest direct anti-papillomaviral effects in vitro, but exerts protective functions against Mus musculus papillomavirus 1 skin infection and disease development in vivo, primarily by modulation of cellular and humoral immunity.
    MeSH term(s) Animals ; Antimicrobial Cationic Peptides ; Cathelicidins ; Mice ; Mice, Inbred C57BL ; Papilloma/chemically induced ; Papillomaviridae/genetics
    Chemical Substances Antimicrobial Cationic Peptides ; Cathelicidins ; ropocamptide (3DD771JO2H)
    Language English
    Publishing date 2021-01-05
    Publishing country Sweden
    Document type Journal Article
    ZDB-ID 80007-7
    ISSN 1651-2057 ; 0001-5555
    ISSN (online) 1651-2057
    ISSN 0001-5555
    DOI 10.2340/00015555-3733
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ui VI Zs.101: Show issues Location:
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  8. Article ; Online: Interaction with Ribosomal Proteins Accompanies Stress Induction of the Anticancer Metallodrug BOLD-100/KP1339 in the Endoplasmic Reticulum.

    Neuditschko, Benjamin / Legin, Anton A / Baier, Dina / Schintlmeister, Arno / Reipert, Siegfried / Wagner, Michael / Keppler, Bernhard K / Berger, Walter / Meier-Menches, Samuel M / Gerner, Christopher

    Angewandte Chemie (International ed. in English)

    2021  Volume 60, Issue 10, Page(s) 5063–5068

    Abstract: The ruthenium-based anticancer agent BOLD-100/KP1339 has shown promising results in several in vitro and in vivo tumour models as well as in early clinical trials. However, its mode of action remains to be fully elucidated. Recent evidence identified ... ...

    Abstract The ruthenium-based anticancer agent BOLD-100/KP1339 has shown promising results in several in vitro and in vivo tumour models as well as in early clinical trials. However, its mode of action remains to be fully elucidated. Recent evidence identified stress induction in the endoplasmic reticulum (ER) and concomitant down-modulation of HSPA5 (GRP78) as key drug effects. By exploiting the naturally formed adduct between BOLD-100 and human serum albumin as an immobilization strategy, we were able to perform target-profiling experiments that revealed the ribosomal proteins RPL10, RPL24, and the transcription factor GTF2I as potential interactors of this ruthenium(III) anticancer agent. Integrating these findings with proteomic profiling and transcriptomic experiments supported ribosomal disturbance and concomitant induction of ER stress. The formation of polyribosomes and ER swelling of treated cancer cells revealed by TEM validated this finding. Thus, the direct interaction of BOLD-100 with ribosomal proteins seems to accompany ER stress-induction and modulation of GRP78 in cancer cells.
    MeSH term(s) Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Endoplasmic Reticulum/drug effects ; Endoplasmic Reticulum Chaperone BiP ; Endoplasmic Reticulum Stress/drug effects ; HCT116 Cells ; Humans ; Organometallic Compounds/chemistry ; Organometallic Compounds/pharmacology ; Polyribosomes/metabolism ; Ribosomal Protein L10/metabolism ; Ribosomal Proteins/metabolism ; Ruthenium/chemistry ; Transcription Factors, TFII/metabolism ; Transcriptome
    Chemical Substances Antineoplastic Agents ; Endoplasmic Reticulum Chaperone BiP ; GTF2I protein, human ; HSPA5 protein, human ; KP 1339 ; Organometallic Compounds ; RPL10 protein, human ; Ribosomal Proteins ; Transcription Factors, TFII ; ribosomal protein L24 ; Ruthenium (7UI0TKC3U5)
    Language English
    Publishing date 2021-02-01
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202015962
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Recently photoassimilated carbon and fungus-delivered nitrogen are spatially correlated in the ectomycorrhizal tissue of Fagus sylvatica.

    Mayerhofer, Werner / Schintlmeister, Arno / Dietrich, Marlies / Gorka, Stefan / Wiesenbauer, Julia / Martin, Victoria / Gabriel, Raphael / Reipert, Siegfried / Weidinger, Marieluise / Clode, Peta / Wagner, Michael / Woebken, Dagmar / Richter, Andreas / Kaiser, Christina

    The New phytologist

    2021  Volume 232, Issue 6, Page(s) 2457–2474

    Abstract: Ectomycorrhizal plants trade plant-assimilated carbon for soil nutrients with their fungal partners. The underlying mechanisms, however, are not fully understood. Here we investigate the exchange of carbon for nitrogen in the ectomycorrhizal symbiosis of ...

    Abstract Ectomycorrhizal plants trade plant-assimilated carbon for soil nutrients with their fungal partners. The underlying mechanisms, however, are not fully understood. Here we investigate the exchange of carbon for nitrogen in the ectomycorrhizal symbiosis of Fagus sylvatica across different spatial scales from the root system to the cellular level. We provided
    MeSH term(s) Carbon ; Fagus ; Mycorrhizae ; Nitrogen ; Plant Roots
    Chemical Substances Carbon (7440-44-0) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2021-08-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 208885-x
    ISSN 1469-8137 ; 0028-646X
    ISSN (online) 1469-8137
    ISSN 0028-646X
    DOI 10.1111/nph.17591
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    In stock of ZB MED Bonn / Germany

    Z 868: Show issues

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  10. Article: Die Wechselwirkung mit ribosomalen Proteinen begleitet die Stressinduktion des Wirkstoffkandidaten BOLD-100/KP1339 im endoplasmatischen Retikulum.

    Neuditschko, Benjamin / Legin, Anton A / Baier, Dina / Schintlmeister, Arno / Reipert, Siegfried / Wagner, Michael / Keppler, Bernhard K / Berger, Walter / Meier-Menches, Samuel M / Gerner, Christopher

    Angewandte Chemie (Weinheim an der Bergstrasse, Germany)

    2021  Volume 133, Issue 10, Page(s) 5121–5126

    Language English
    Publishing date 2021-02-01
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 506609-8
    ISSN 1521-3757 ; 0044-8249 ; 0932-2140
    ISSN (online) 1521-3757
    ISSN 0044-8249 ; 0932-2140
    DOI 10.1002/ange.202015962
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    In stock of ZB MED Cologne/Königswinter

    Ui VI 2770: Show issues Location:
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