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Article ; Online: Photograph registration of petri dishes in high quality and image editing of microbiological assays.

Rodrigues, Cleverson / David, Grace Queiroz / Dos Reis, André Rodrigues

International microbiology : the official journal of the Spanish Society for Microbiology

2022 Volume 25, Issue 3, Page(s) 561–570

Abstract: Science is based on evidence that can be measured or observed through methodical techniques which are expressed in several ways, either quantitatively or qualitatively. Technical photograph becomes one of the most important key tools to disclose ... ...

Abstract	Science is based on evidence that can be measured or observed through methodical techniques which are expressed in several ways, either quantitatively or qualitatively. Technical photograph becomes one of the most important key tools to disclose experimental results. In microbiological research, several pieces of evidence can be indicated with parameters that are deeply related to culture media; pH and color variation, halo formation, overlay of structures, culture shape, among others. The employment of technical photographs as a strategy of the experimental observation and reliable representation is indispensable. The protocol presented here suggests the production of photographic support in microbiological assays in Petri dishes taken by smartphone to obtain high-quality images, besides showing tools to edit images using PowerPoint. The support is composed of a paper tube with a transparent border, whose reduced light penetration avoiding light reflection over the Petri dishes or the culture media. The edition consists of photograph variation, and in clipping and pasting on uniform backgrounds to provide further detailing. The protocol allowed a standardized photograph collection in high quality, which is ideal for a comparative portrait of microbiological behaviors. The image editing enabled a framework and greater visibility of physical and biological structures in the exhibition of photographs inside the manuscript, such as the removal of noises, background alterations, deformities or irregularities. This protocol is an intelligent and cheap tool to help researcher on the knowledge-obtaining process, and it is applied to different experiments or adapted into the most variable research subjects.
MeSH term(s)	Culture Media ; Humans
Chemical Substances	Culture Media
Language	English
Publishing date	2022-03-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1454951-7
ISSN	1618-1905 ; 1139-6709
ISSN (online)	1618-1905
ISSN	1139-6709
DOI	10.1007/s10123-022-00242-z
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Book ; Online ; Thesis: Genetic risk factors of generalized pustular psoriasis and their underlying disease mechanisms

Haskamp, Stefan [Verfasser] / Hüffmeier, Ulrike [Akademischer Betreuer] / Reis, André [Gutachter]

2023

Author's details	Stefan Haskamp ; Gutachter: André Reis ; Betreuer: Ulrike Hüffmeier
Keywords	Naturwissenschaften ; Science
Subject code	sg500
Language	English
Publisher	Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Publishing place	Erlangen
Document type	Book ; Online ; Thesis
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Book ; Online ; Thesis: Aufklärung genetischer Ursachen und Pathomechanismen psychomotorischer Entwicklungsstörungen

Büttner, Jana [Verfasser] / Reis, André [Akademischer Betreuer] / Reis, André [Gutachter]

2020

Author's details	Jana Büttner ; Gutachter: André Reis ; Betreuer: André Reis
Keywords	Medizin, Gesundheit ; Medicine, Health
Subject code	sg610
Language	German
Publisher	Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Publishing place	Erlangen
Document type	Book ; Online ; Thesis
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Article ; Online: Severe manifestation of Rauch-Azzarello syndrome associated with biallelic deletion of CTNND2.

Pauly, Melissa / Krumbiegel, Mandy / Trumpp, Sandra / Braig, Sonja / Rupprecht, Thomas / Kraus, Cornelia / Uebe, Steffen / Reis, André / Vasileiou, Georgia

Clinical genetics

2024

Abstract: CTNND2 encodes δ-catenin, a component of an adherens junction complex, and plays an important role in neuronal structure and function. To date, only heterozygous loss-of-function CTNND2 variants have been associated with mild neurodevelopmental delay and ...

Abstract	CTNND2 encodes δ-catenin, a component of an adherens junction complex, and plays an important role in neuronal structure and function. To date, only heterozygous loss-of-function CTNND2 variants have been associated with mild neurodevelopmental delay and behavioral anomalies, a condition, which we named Rauch-Azzarello syndrome. Here, we report three siblings of a consanguineous family of Syrian descent with a homozygous deletion encompassing the last 19 exons of CTNND2 predicted to disrupt the transcript. All presented with severe neurodevelopmental delay with absent speech, profound motor delay, stereotypic behavior, microcephaly, short stature, muscular hypotonia with lower limb hypertonia, and variable eye anomalies. The parents and the fourth sibling were heterozygous carriers of the deletion and exhibited mild neurodevelopmental impairment resembling that of the previously described heterozygous individuals. The present study unveils a severe manifestation of CTNND2-associated Rauch-Azzarello syndrome attributed to biallelic loss-of-function aberrations, clinically distinct from the already described mild presentation of heterozygous individuals. Furthermore, we demonstrate novel clinical features in homozygous individuals that have not been reported in heterozygous cases to date.
Language	English
Publishing date	2024-04-11
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	221209-2
ISSN	1399-0004 ; 0009-9163
ISSN (online)	1399-0004
ISSN	0009-9163
DOI	10.1111/cge.14532
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Article ; Online: Roles of selenium in mineral plant nutrition: ROS scavenging responses against abiotic stresses.

Lanza, Maria Gabriela Dantas Bereta / Reis, André Rodrigues Dos

Plant physiology and biochemistry : PPB

2021 Volume 164, Page(s) 27–43

Abstract: Agronomic biofortification of crops with selenium (Se) is an important strategy to minimize hidden hunger and increase nutrient intake in poor populations. Selenium is an element that has several physiological and biochemical characteristics, such as the ...

Abstract	Agronomic biofortification of crops with selenium (Se) is an important strategy to minimize hidden hunger and increase nutrient intake in poor populations. Selenium is an element that has several physiological and biochemical characteristics, such as the mitigation of different types of abiotic stress. Selenoproteins act as powerful antioxidants in plant metabolism through the glutathione peroxidase (GSH) pathway, and provide an increased activity for enzymatic (SOD, CAT, and APX) and non-enzymatic (ascorbic acid, flavonoids, and tocopherols) compounds that act in reactive oxygen species (ROS) scavenging system and cell detoxification. Selenium helps to inhibit the damage caused by climate changes such as drought, salinity, heavy metals, and extreme temperature. Also, Se regulates antenna complex of photosynthesis, protecting chlorophylls by raising photosynthetic pigments. However, Se concentrations in soils vary widely in the earth's crust. Soil Se availability regulates the uptake, transport, accumulation, and speciation in plants. Foliar Se application at the concentration 50 g ha
MeSH term(s)	Antioxidants ; Nutritional Status ; Reactive Oxygen Species ; Selenic Acid ; Selenium ; Stress, Physiological
Chemical Substances	Antioxidants ; Reactive Oxygen Species ; Selenium (H6241UJ22B) ; Selenic Acid (HV0Y51NC4J)
Language	English
Publishing date	2021-04-29
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2021.04.026
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Article ; Online: Influence of Er:YAG laser irradiation settings on dentin-adhesive interfacial ultramorphology and dentin bond strength.

Vermelho, Paulo Moreira / Freitas, Patricia Moreira / Reis, André Figueiredo / Giannini, Marcelo

Microscopy research and technique

2022 Volume 85, Issue 8, Page(s) 2943–2952

Abstract: This study evaluated the effects of Erbium-doped yttrium aluminium garnet (Er:YAG) laser settings and dentin bonding agents on ultramorphological characteristics of resin-laser-irradiated dentin interfaces and dentin bond strength (BS) of these adhesive ... ...

Abstract	This study evaluated the effects of Erbium-doped yttrium aluminium garnet (Er:YAG) laser settings and dentin bonding agents on ultramorphological characteristics of resin-laser-irradiated dentin interfaces and dentin bond strength (BS) of these adhesive systems. Additionally, dentin depth affected by Er:YAG laser irradiations was measured. The experiments were performed on occlusal dentin surfaces of third molars that were flattened with 600-grit SiC sandpaper. Treated-dentin with laser settings (250 mJ/4 Hz and 160 mJ/10 Hz) were the experimental groups, while SiC abraded dentin was the control. These three dentin treatments and three adhesives (two self-etchings and one etch-&-rinse adhesive) formed nine groups for the ultramorphology of laser-ablated dentin-adhesives interfacial analysis, using a transmission electron microscope (TEM). For BS (n = 8), the same nine groups were tested with addition of the two evaluation times (24 h after sample preparation or 1 year). The depths of Er:YAG laser effects into the dentin were measured using a TEM (n = 10). Ablated-dentin depth and BS data were analyzed by one- and three-way ANOVA, respectively, and Tukey's test (α = 0.05). Hybrid layer formation was only observed for controls, while for laser-treated dentin, adhesives were bonded to dentin with resin tags formation. Laser settings reduced the BS for all adhesives at 24 h, while at 1 year, etch-&-rinse adhesive presented the highest BS, regardless treatment (control or laser settings). Dentin depth affected by laser settings was similar. The laser irradiation altered the bonding mechanism of the adhesives to dentin and reduced the BS for self-etching adhesives. Etch-&-rinse adhesive yielded the highest BS at 1 year. Laser settings similarly affected the dentin in depth. HIGHLIGHTS: Er:YAG laser irradiation settings produced similar effects on depth and bond strength to dentin. The etch-&-rinse adhesive yielded the highest dentin bond strength regardless of the type of dentin treatment at 1 year.
MeSH term(s)	Adhesives/analysis ; Dental Bonding ; Dentin/chemistry ; Dentin-Bonding Agents/chemistry ; Erbium ; Lasers, Solid-State/therapeutic use ; Resin Cements/chemistry ; Tensile Strength
Chemical Substances	Adhesives ; Dentin-Bonding Agents ; Resin Cements ; Erbium (77B218D3YE)
Language	English
Publishing date	2022-05-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	1099714-3
ISSN	1097-0029 ; 1059-910X
ISSN (online)	1097-0029
ISSN	1059-910X
DOI	10.1002/jemt.24144
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Article ; Online: Physiological and biochemical roles of ascorbic acid on mitigation of abiotic stresses in plants.

Celi, Gabriela Eugenia Ajila / Gratão, Priscila Lupino / Lanza, Maria Gabriela Dantas Bereta / Reis, André Rodrigues Dos

Plant physiology and biochemistry : PPB

2023 Volume 202, Page(s) 107970

Abstract: Under conditions of abiotic stress several physiological and biochemical processes in plants can be modified. The production of reactive oxygen species (ROS) is toxic at high concentrations and promotes RNA, DNA and plant cell membrane degradation. ... ...

Abstract	Under conditions of abiotic stress several physiological and biochemical processes in plants can be modified. The production of reactive oxygen species (ROS) is toxic at high concentrations and promotes RNA, DNA and plant cell membrane degradation. Plants have enzymatic and non-enzymatic adaptation mechanisms to act against ROS detoxification. Ascorbic acid (AsA) is the non-enzymatic compound essential for several biological functions, which acts in the elimination and balance of ROS production and with the potential to promote several physiological functions in plants, such as the photosynthetic process. For plant development, AsA plays an important role in cell division, osmotic adjustment, hormone biosynthesis, and as an enzymatic cofactor. In this review, the redox reactions, biosynthetic pathways, and the physiological and biochemical functions of AsA against abiotic stress in plants are discussed. The concentration of AsA in plants can vary between species and depend on the biosynthetic pathways d-mannose/l-galactose, d-galacturonate, euglenids, and d-glucuronate. Although the endogenous levels of AsA in plants are used in large amounts in cell metabolism, the exogenous application of AsA further increases these endogenous levels to promote the antioxidant system and ameliorate the effects produced by abiotic stress. Foliar application of AsA promotes antioxidant metabolism in plants subjected to climate change conditions, also allowing the production of foods with higher nutritional quality and food safety, given the fact that AsA is biologically essential in the human diet.
MeSH term(s)	Humans ; Ascorbic Acid ; Antioxidants ; Reactive Oxygen Species ; Acclimatization ; Cell Division
Chemical Substances	Ascorbic Acid (PQ6CK8PD0R) ; Antioxidants ; Reactive Oxygen Species
Language	English
Publishing date	2023-08-16
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2023.107970
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Article ; Online: Reflectance Measurements from Aerial and Proximal Sensors Provide Similar Precision in Predicting the Rice Yield Response to Mid-Season N Applications.

Rehman, Telha H / Lundy, Mark E / Reis, Andre Froes de Borja / Akbar, Nadeem / Linquist, Bruce A

Sensors (Basel, Switzerland)

2023 Volume 23, Issue 13

Abstract: Accurately detecting nitrogen (N) deficiency and determining the need for additional N fertilizer is a key challenge to achieving precise N management in many crops, including rice ( ...

Abstract	Accurately detecting nitrogen (N) deficiency and determining the need for additional N fertilizer is a key challenge to achieving precise N management in many crops, including rice (
MeSH term(s)	Oryza ; Fertilizers/analysis ; Seasons ; Environment ; Nitrogen
Chemical Substances	Fertilizers ; Nitrogen (N762921K75)
Language	English
Publishing date	2023-07-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s23136218
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Article ; Online: Expanding the phenotype of 12q21 deletions: A role of BTG1 in speech development?

Blum, Katalin Lml / Krumbiegel, Mandy / Kraus, Cornelia / Reis, André / Hüffmeier, Ulrike

European journal of medical genetics

2023 Volume 66, Issue 4, Page(s) 104717

Abstract: We report on a female individual with feeding difficulties, constipation, poor overall growth, periventricular lesions resembling gliosis in brain MRI, recurrent otitis media with palsy of facial nerve, distinct facial features, and pronounced delay in ... ...

Abstract	We report on a female individual with feeding difficulties, constipation, poor overall growth, periventricular lesions resembling gliosis in brain MRI, recurrent otitis media with palsy of facial nerve, distinct facial features, and pronounced delay in speech development. The latter was the most prominent feature. Molecular karyotyping revealed a heterozygous de novo deletion of 4.353 Mb at chromosome 12q21.33q22. This report expands the number of described individuals with heterozygous deletions at 12q21.33, their clinical spectrum and highlights the clinical variability, even in individuals with deletion of the same genes. Furthermore, our findings indicate a role of BTG1 (OMIM *109580) in speech development.
MeSH term(s)	Child ; Humans ; Female ; Chromosome Deletion ; Developmental Disabilities/genetics ; Speech ; Phenotype ; Karyotyping ; Neoplasm Proteins/genetics
Chemical Substances	BTG1 protein, human (146835-72-5) ; Neoplasm Proteins
Language	English
Publishing date	2023-02-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2184135-4
ISSN	1878-0849 ; 1769-7212
ISSN (online)	1878-0849
ISSN	1769-7212
DOI	10.1016/j.ejmg.2023.104717
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Article ; Online: Selenium enhances ROS scavenging systems and sugar metabolism increasing growth of sugarcane plants.

Araujo, Maycon Anderson de / Melo, Andressa Aparecida Rodrigues de / Silva, Vinicius Martins / Reis, André Rodrigues Dos

Plant physiology and biochemistry : PPB

2023 Volume 201, Page(s) 107798

Abstract: Selenium (Se) beneficial effect on plants is related to an increase in nitrogen (N) assimilation and its role as an abiotic stress mitigator by reactive oxygen species (ROS) scavenging enhanced by antioxidant metabolism. This study aimed to evaluate ... ...

Abstract	Selenium (Se) beneficial effect on plants is related to an increase in nitrogen (N) assimilation and its role as an abiotic stress mitigator by reactive oxygen species (ROS) scavenging enhanced by antioxidant metabolism. This study aimed to evaluate sugarcane (Saccharum spp.) growth, photosynthetic and antioxidant responses, and sugar accumulation in response to Se supply. The experimental design was a factorial scheme 2 × 4: two sugarcane varieties (RB96 6928 and RB86 7515) and four Se application rates (0; 5; 10 and 20 μmol L
MeSH term(s)	Selenium/metabolism ; Antioxidants/metabolism ; Saccharum/metabolism ; Reactive Oxygen Species/metabolism ; Carbon Dioxide/metabolism ; Edible Grain/metabolism ; Sugars/metabolism ; Plant Leaves/metabolism
Chemical Substances	Selenium (H6241UJ22B) ; Antioxidants ; Reactive Oxygen Species ; Carbon Dioxide (142M471B3J) ; Sugars
Language	English
Publishing date	2023-06-04
Publishing country	France
Document type	Journal Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2023.107798
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