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  1. Article ; Online: Multi-stemming enhances tree survival and growth in Borneo’s logged forests

    Kvasnica, Jakub / Matula, Radim / Rejžek, Martin / Ewers, Robert M. / Riutta, Terhi / Turner, Edgar C. / Nilus, Reuben / Svátek, Martin

    Forest Ecology and Management. 2023 Sept., v. 544 p.121140-

    2023  

    Abstract: Multi-stemmed architecture is a common but overlooked tree growth form in tropical rainforests. Although multi-stemming is thought to be a key trait for tree persistence after disturbance, the role of multi-stemmed trees in post-disturbance demographic ... ...

    Abstract Multi-stemmed architecture is a common but overlooked tree growth form in tropical rainforests. Although multi-stemming is thought to be a key trait for tree persistence after disturbance, the role of multi-stemmed trees in post-disturbance demographic processes has been largely unexplored in logged rainforests. Here, we used decade-long measurements on nearly 14,000 tree stems to study post-logging growth and survival along a logging disturbance gradient in Malaysian Borneo. We found that multi-stemming enhanced tree persistence after logging. Along the entire disturbance gradient, multi-stemmed trees suffered significantly lower mortality than single-stemmed trees, and survival strongly increased with the number of stems. Small to medium-sized trees with multiple stems showed greater growth than similar-sized single-stemmed trees, with the difference increasing as the disturbance increased. Our findings demonstrate the significance of multi-stemmed architecture after logging in Borneo’s rainforests and highlight the need to consider the multi-stemmed growth form in forest inventories and models of post-disturbance forest dynamics.
    Keywords Borneo ; administrative management ; forest dynamics ; forests ; mortality ; tree growth ; trees ; Multi-stemmed trees ; Resprouting ; Disturbance gradient ; Logging
    Language English
    Dates of publication 2023-09
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 751138-3
    ISSN 0378-1127
    ISSN 0378-1127
    DOI 10.1016/j.foreco.2023.121140
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Exploration of the Transglycosylation Activity of Barley Limit Dextrinase for Production of Novel Glycoconjugates.

    Vester-Christensen, Malene Bech / Holck, Jesper / Rejzek, Martin / Perrin, Léa / Tovborg, Morten / Svensson, Birte / Field, Robert A / Møller, Marie Sofie

    Molecules (Basel, Switzerland)

    2023  Volume 28, Issue 10

    Abstract: A few α-glucan debranching enzymes (DBEs) of the large glycoside hydrolase family 13 (GH13), also known as the α-amylase family, have been shown to catalyze transglycosylation as well as hydrolysis. However, little is known about their acceptor and donor ...

    Abstract A few α-glucan debranching enzymes (DBEs) of the large glycoside hydrolase family 13 (GH13), also known as the α-amylase family, have been shown to catalyze transglycosylation as well as hydrolysis. However, little is known about their acceptor and donor preferences. Here, a DBE from barley, limit dextrinase (
    MeSH term(s) Hordeum/metabolism ; Glycoside Hydrolases/metabolism ; Cyclodextrins ; Hydrolysis ; Substrate Specificity
    Chemical Substances pullulanase (EC 3.2.1.41) ; Glycoside Hydrolases (EC 3.2.1.-) ; Cyclodextrins
    Language English
    Publishing date 2023-05-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules28104111
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Identification of a Kdn biosynthesis pathway in the haptophyte

    Wagstaff, Ben A / Rejzek, Martin / Field, Robert A

    The Journal of biological chemistry

    2018  Volume 293, Issue 42, Page(s) 16277–16290

    Abstract: Sialic acids are a family of more than 50 structurally distinct acidic sugars on the surface of all vertebrate cells where they terminate glycan chains and are exposed to many interactions with the surrounding environment. In particular, sialic acids ... ...

    Abstract Sialic acids are a family of more than 50 structurally distinct acidic sugars on the surface of all vertebrate cells where they terminate glycan chains and are exposed to many interactions with the surrounding environment. In particular, sialic acids play important roles in cell-cell and host-pathogen interactions. The sialic acids or related nonulosonic acids have been observed in Deuterostome lineages, Eubacteria, and Archaea but are notably absent from plants. However, the structurally related C8 acidic sugar 3-deoxy-d-
    MeSH term(s) Biosynthetic Pathways ; Cytidine Monophosphate/analogs & derivatives ; Cytidine Monophosphate/biosynthesis ; Haptophyta/metabolism ; Microalgae/metabolism ; N-Acetylneuraminic Acid/biosynthesis ; Neuraminic Acids
    Chemical Substances Neuraminic Acids ; CMP-deaminoneuraminic acid (137463-62-8) ; Cytidine Monophosphate (F469818O25) ; N-Acetylneuraminic Acid (GZP2782OP0)
    Language English
    Publishing date 2018-08-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1074/jbc.RA118.004921
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Complete biosynthesis of the potent vaccine adjuvant QS-21.

    Martin, Laetitia B B / Kikuchi, Shingo / Rejzek, Martin / Owen, Charlotte / Reed, James / Orme, Anastasia / Misra, Rajesh C / El-Demerdash, Amr / Hill, Lionel / Hodgson, Hannah / Liu, Yuzhong / Keasling, Jay D / Field, Robert A / Truman, Andrew W / Osbourn, Anne

    Nature chemical biology

    2024  Volume 20, Issue 4, Page(s) 493–502

    Abstract: QS-21 is a potent vaccine adjuvant currently sourced by extraction from the Chilean soapbark tree. It is a key component of human vaccines for shingles, malaria, coronavirus disease 2019 and others under development. The structure of QS-21 consists of a ... ...

    Abstract QS-21 is a potent vaccine adjuvant currently sourced by extraction from the Chilean soapbark tree. It is a key component of human vaccines for shingles, malaria, coronavirus disease 2019 and others under development. The structure of QS-21 consists of a glycosylated triterpene scaffold coupled to a complex glycosylated 18-carbon acyl chain that is critical for immunostimulant activity. We previously identified the early pathway steps needed to make the triterpene glycoside scaffold; however, the biosynthetic route to the acyl chain, which is needed for stimulation of T cell proliferation, was unknown. Here, we report the biogenic origin of the acyl chain, characterize the series of enzymes required for its synthesis and addition and reconstitute the entire 20-step pathway in tobacco, thereby demonstrating the production of QS-21 in a heterologous expression system. This advance opens up unprecedented opportunities for bioengineering of vaccine adjuvants, investigating structure-activity relationships and understanding the mechanisms by which these compounds promote the human immune response.
    MeSH term(s) Humans ; Adjuvants, Vaccine ; Saponins/pharmacology ; Adjuvants, Immunologic/pharmacology ; Adjuvants, Immunologic/chemistry ; Triterpenes
    Chemical Substances saponin QA-21V1 (61H83WZX3U) ; Adjuvants, Vaccine ; Saponins ; Adjuvants, Immunologic ; Triterpenes
    Language English
    Publishing date 2024-01-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2202962-X
    ISSN 1552-4469 ; 1552-4450
    ISSN (online) 1552-4469
    ISSN 1552-4450
    DOI 10.1038/s41589-023-01538-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines

    Emmrich, Peter M.F. / Rejzek, Martin / Hill, Lionel / Brett, Paul / Edwards, Anne / Sarkar, Abhimanyu / Field, Rob A. / Martin, Cathie / Wang, Trevor L.

    BMC Plant Biology

    2023  

    Abstract: Grass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3- ... ...

    Abstract Grass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties.
    Keywords identification ; permits ; pea
    Publishing date 2023-03-10T14:41:21Z
    Publisher Springer
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Heterologous Expression of the Formicamycin Biosynthetic Gene Cluster Unveils Glycosylated Fasamycin Congeners.

    McDonald, Hannah P / Alford, Abigail / Devine, Rebecca / Hems, Edward S / Nepogodiev, Sergey A / Arnold, Corinne J / Rejzek, Martin / Stanley-Smith, Anna / Holmes, Neil A / Hutchings, Matthew I / Wilkinson, Barrie

    Journal of natural products

    2023  Volume 86, Issue 7, Page(s) 1677–1689

    Abstract: Formicamycins and their biosynthetic intermediates the fasamycins are polyketide antibiotics produced ... ...

    Abstract Formicamycins and their biosynthetic intermediates the fasamycins are polyketide antibiotics produced by
    MeSH term(s) Galactose/metabolism ; Anti-Bacterial Agents/metabolism ; Streptomyces coelicolor/genetics ; Multigene Family ; Glucose/metabolism
    Chemical Substances Galactose (X2RN3Q8DNE) ; Anti-Bacterial Agents ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2023-06-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 304325-3
    ISSN 1520-6025 ; 0163-3864
    ISSN (online) 1520-6025
    ISSN 0163-3864
    DOI 10.1021/acs.jnatprod.3c00052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Chemoenzymatic Synthesis of C6-Modified Sugar Nucleotides To Probe the GDP-d-Mannose Dehydrogenase from Pseudomonas aeruginosa

    Ahmadipour, Sanaz / Field, Robert A / Miller, Gavin J / Pergolizzi, Giulia / Rejzek, Martin

    Organic letters. 2019 May 30, v. 21, no. 12

    2019  

    Abstract: The chemoenzymatic synthesis of a series of C6-modified GDP-d-Man sugar nucleotides is described. This provides the first structure–function tools for the GDP-d-ManA producing GDP-d-mannose dehydrogenase (GMD) from Pseudomonas aeruginosa. Using a common ... ...

    Abstract The chemoenzymatic synthesis of a series of C6-modified GDP-d-Man sugar nucleotides is described. This provides the first structure–function tools for the GDP-d-ManA producing GDP-d-mannose dehydrogenase (GMD) from Pseudomonas aeruginosa. Using a common C6 aldehyde functionalization strategy, chemical synthesis introduces deuterium enrichment, alongside one-carbon homologation at C6 for a series of mannose 1-phosphates. These materials are shown to be substrates for the GDP-mannose pyrophosphorylase from Salmonella enterica, delivering the required toolbox of modified GDP-d-Mans. C6-CH3 modified sugar-nucleotides are capable of reversibly preventing GDP-ManA production by GMD. The ketone product from oxidation of a C6-CH3 modified analogue is identified by high-resolution mass spectrometry.
    Keywords aldehydes ; chemical structure ; deuterium ; enzymes ; mannose ; mass spectrometry ; nucleotides ; oxidation ; Pseudomonas aeruginosa ; Salmonella enterica ; synthesis
    Language English
    Dates of publication 2019-0530
    Size p. 4415-4419.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1523-7052
    DOI 10.1021/acs.orglett.9b00967
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Synthesis of glyceryl glycosides related to A-type prymnesin toxins.

    Hems, Edward S / Nepogodiev, Sergey A / Rejzek, Martin / Field, Robert A

    Carbohydrate research

    2018  Volume 463, Page(s) 14–23

    Abstract: A suite of glycosylated glycerol derivatives representing various fragments of the glycosylated ichthyotoxins called prymnesins were chemically synthesised. Glycerol was used to represent a small fragment of the prymnesin backbone, and was glycosylated ... ...

    Abstract A suite of glycosylated glycerol derivatives representing various fragments of the glycosylated ichthyotoxins called prymnesins were chemically synthesised. Glycerol was used to represent a small fragment of the prymnesin backbone, and was glycosylated at the 2° position with the sugars currently reported to be present on prymnesin toxins. Neighbouring group participation was utilised to synthesise 1,2-trans-glycosides. SnCl
    MeSH term(s) Glycosides/chemical synthesis ; Glycosides/chemistry ; Glycosylation ; Models, Molecular ; Molecular Structure ; Polyketides ; Pyrans/chemistry ; Stereoisomerism
    Chemical Substances Glycosides ; Polyketides ; Pyrans ; prymnesin 1
    Language English
    Publishing date 2018-04-17
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1435-7
    ISSN 1873-426X ; 0008-6215
    ISSN (online) 1873-426X
    ISSN 0008-6215
    DOI 10.1016/j.carres.2018.04.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Corrigendum to "Synthesis of glyceryl glycosides related to A-type prymnesin toxins" Carbohydr. Res. 463 (2018) 14-23.

    Hems, Edward S / Nepogodiev, Sergey A / Rejzek, Martin / Field, Robert A

    Carbohydrate research

    2018  Volume 470, Page(s) 26

    Language English
    Publishing date 2018-10-09
    Publishing country Netherlands
    Document type Journal Article ; Published Erratum
    ZDB-ID 1435-7
    ISSN 1873-426X ; 0008-6215
    ISSN (online) 1873-426X
    ISSN 0008-6215
    DOI 10.1016/j.carres.2018.09.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Assessing the Toxicity and Mitigating the Impact of Harmful Prymnesium Blooms in Eutrophic Waters of the Norfolk Broads

    Wagstaff, Ben A. / Pratscher, Jennifer / Rivera, Peter Paolo L. / Hems, Edward S. / Brooks, Elliot / Rejzek, Martin / Todd, Jonathan D. / Murrell, J. Colin / Field, Robert A.

    Environmental science & technology. 2021 Dec. 09, v. 55, no. 24

    2021  

    Abstract: Prymnesium parvum is a toxin-producing microalga, which causes harmful algal blooms globally, frequently leading to massive fish kills that have adverse ecological and economic implications for natural waterways and aquaculture alike. The dramatic ... ...

    Abstract Prymnesium parvum is a toxin-producing microalga, which causes harmful algal blooms globally, frequently leading to massive fish kills that have adverse ecological and economic implications for natural waterways and aquaculture alike. The dramatic effects observed on fish are thought to be due to algal polyether toxins, known as the prymnesins, but their lack of environmental detection has resulted in an uncertainty about the true ichthyotoxic agents. Using qPCR, we found elevated levels of P. parvum and its lytic virus, PpDNAV-BW1, in a fish-killing bloom on the Norfolk Broads, United Kingdom, in March 2015. We also detected, for the first time, the B-type prymnesin toxins in Broads waterway samples and gill tissue isolated from a dead fish taken from the study site. Furthermore, Norfolk Broads P. parvum isolates unambiguously produced B-type toxins in laboratory-grown cultures. A 2 year longitudinal study of the Broads study site showed P. parvum blooms to be correlated with increased temperature and that PpDNAV plays a significant role in P. parvum bloom demise. Finally, we used a field trial to show that treatment with low doses of hydrogen peroxide represents an effective strategy to mitigate blooms of P. parvum in enclosed water bodies.
    Keywords Prymnesium parvum ; aquaculture ; eutrophication ; field experimentation ; fish ; hydrogen peroxide ; longitudinal studies ; microalgae ; poisonous algae ; temperature ; toxicity ; uncertainty ; viruses ; United Kingdom
    Language English
    Dates of publication 2021-1209
    Size p. 16538-16551.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1520-5851
    DOI 10.1021/acs.est.1c04742
    Database NAL-Catalogue (AGRICOLA)

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