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  1. Article ; Online: Pleuran-β-Glucan from Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes), Soothes and Improves Skin Parameters.

    Schiano, Irene / Raco, Stefania / Cestone, Enza / Jesenak, Milos / Rennerova, Zuzana / Majtan, Juraj

    International journal of medicinal mushrooms

    2022  Volume 23, Issue 12, Page(s) 75–83

    Abstract: Ultraviolet (UV) radiation, particularly UVB radiation, is one of the major risk factors for environmentally influenced skin disorders. β-D-glucans represent a class of natural compounds, and their use in the cosmetic and pharmaceutical industries is ... ...

    Abstract Ultraviolet (UV) radiation, particularly UVB radiation, is one of the major risk factors for environmentally influenced skin disorders. β-D-glucans represent a class of natural compounds, and their use in the cosmetic and pharmaceutical industries is increasing. The goal of this study was to evaluate the soothing effect of a β-glucan pleuran-based cream against skin alterations caused by a solar simulator (UVA/UVB exposure) and to assess the efficacy of the cream after 30 days of use on the face and body in 20 human subjects with all skin types (sensitive, atopic, and normal) and phototypes II and III. The study consisted of the following two tests: one short term and one long term. In the short-term test, the minimal erythemal dose was determined by applying a pattern of radiation consisting of six doses of UVA+UVB radiation and was visually assessed 24 h after UV exposure. In the long-term test, pleuran-based cream was applied twice a day on both the nonirradiated face and body of all subjects for a 30-day period. Subsequently, noninvasive techniques such as skin moisture, skin brightness/radiance, skin elasticity, and total antioxidant capability were utilized. The pleuran-based cream had a positive effect on erythema induced by UV exposure, with visibly reduced erythema compared to the control area. Moreover, long-term use of the cream over a 30-day period improved all monitored skin parameters on the face and body. In summary, these results indicate that β-glucans can be used as an active ingredient with UV light-protective and soothing properties after UV exposure for the cosmetic and pharmaceutical industries.
    MeSH term(s) Animals ; Antioxidants/pharmacology ; Glucans/pharmacology ; Humans ; Ostreidae ; Pleurotus ; Skin ; Ultraviolet Rays/adverse effects ; beta-Glucans/pharmacology
    Chemical Substances Antioxidants ; Glucans ; beta-Glucans
    Language English
    Publishing date 2022-04-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2108803-2
    ISSN 1940-4344 ; 1521-9437
    ISSN (online) 1940-4344
    ISSN 1521-9437
    DOI 10.1615/IntJMedMushrooms.2021041519
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Beta-(1,3/1,6)-D-glucan from

    Rennerova, Zuzana / Picó Sirvent, Leandro / Carvajal Roca, Eva / Paśnik, Jarosław / Logar, Mateja / Milošević, Katarina / Majtan, Juraj / Jesenak, Milos

    Frontiers in pediatrics

    2022  Volume 10, Page(s) 999701

    Abstract: Preschool children are particularly susceptible to recurrent upper and lower respiratory tract infections due to their immune immaturity and other contributing factors. Preventing and/or treating children suffering from recurrent respiratory tract ... ...

    Abstract Preschool children are particularly susceptible to recurrent upper and lower respiratory tract infections due to their immune immaturity and other contributing factors. Preventing and/or treating children suffering from recurrent respiratory tract infections (RRTIs) is challenging, and it is important to provide more clinical evidence about the safety and efficacy of natural immunomodulating preparations, including β-glucans. The aim of the present study was to assess the incidence of respiratory tract infections (RTIs) in children with a history of RRTIs for a period of 6 months (3 months of pleuran supplementation and 3 months of follow-up) compared with the same period from October to March of the previous year prior to enrolment in the study. A total of 1,030 children with a mean age of 3.49 ± 1.91 years from seven countries were included in this study. The total number of RTIs observed during the study period was significantly lower compared to the same period of the previous year (7.07 ± 2.89 vs. 3.87 ± 3.19;
    Language English
    Publishing date 2022-10-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711999-3
    ISSN 2296-2360
    ISSN 2296-2360
    DOI 10.3389/fped.2022.999701
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Efficacy of Pleuran (

    Urbancikova, Ingrid / Hudackova, Dana / Majtan, Juraj / Rennerova, Zuzana / Banovcin, Peter / Jesenak, Milos

    Evidence-based complementary and alternative medicine : eCAM

    2020  Volume 2020, Page(s) 8562309

    Abstract: One of the highly prevalent viral pathogens among children and adults causing infection, clinically presenting as herpes labialis, is herpes simplex virus type 1 (HSV-1). The long-term administration of acyclovir, a standard regimen for therapy against ... ...

    Abstract One of the highly prevalent viral pathogens among children and adults causing infection, clinically presenting as herpes labialis, is herpes simplex virus type 1 (HSV-1). The long-term administration of acyclovir, a standard regimen for therapy against HSV-1 infections, can cause viral resistance against this drug. Therefore, the development of natural drugs with low toxicity that are able to enhance host antiviral defense against HSV infection is needed.
    Language English
    Publishing date 2020-04-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2020/8562309
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Immune Parameters and COVID-19 Infection - Associations With Clinical Severity and Disease Prognosis.

    Jesenak, Milos / Brndiarova, Miroslava / Urbancikova, Ingrid / Rennerova, Zuzana / Vojtkova, Jarmila / Bobcakova, Anna / Ostro, Robert / Banovcin, Peter

    Frontiers in cellular and infection microbiology

    2020  Volume 10, Page(s) 364

    Abstract: Severe acute respiratory syndrome caused by a novel 2019 coronavirus (SARS-CoV2) represents one of the most studied infectious diseases of today. The number of scientific reports and publications increases exponentially day by day. While the majority of ... ...

    Abstract Severe acute respiratory syndrome caused by a novel 2019 coronavirus (SARS-CoV2) represents one of the most studied infectious diseases of today. The number of scientific reports and publications increases exponentially day by day. While the majority of infected subjects are asymptomatic or show mild symptoms, there is an important proportion of patients who requires hospitalization and, sometimes, intensive care. Immune response to novel coronavirus is complex, involves both innate and adaptive immunity, and is biphasic. Significant differences were observed when comparing severe and non-severe patients. Analysis of the reported results from clinical trials clearly show an involvement of specific cellular immunity (predominantly leucopenia, decreased counts of CD3
    MeSH term(s) Betacoronavirus/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; COVID-19 ; Coronavirus Infections/immunology ; Coronavirus Infections/pathology ; Cytokine Release Syndrome/immunology ; Cytokines/immunology ; Eosinophils/immunology ; Humans ; Lung/pathology ; Pandemics ; Pneumonia, Viral/immunology ; Pneumonia, Viral/pathology ; Prognosis ; SARS-CoV-2 ; Severity of Illness Index
    Chemical Substances Cytokines
    Keywords covid19
    Language English
    Publishing date 2020-06-30
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2020.00364
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: β-Glucans in the treatment and prevention of allergic diseases.

    Jesenak, Milos / Banovcin, Peter / Rennerova, Zuzana / Majtan, Juraj

    Allergologia et immunopathologia

    2014  Volume 42, Issue 2, Page(s) 149–156

    Abstract: β-glucans are a group of biologically active polysaccharides of natural origin with a proven pleiotropic immunomodulation effect. Their efficacy has been confirmed in the therapeutic treatment and prevention of various infectious diseases, secondary ... ...

    Abstract β-glucans are a group of biologically active polysaccharides of natural origin with a proven pleiotropic immunomodulation effect. Their efficacy has been confirmed in the therapeutic treatment and prevention of various infectious diseases, secondary immune defects and also of oncologic disorders. Allergic diseases are one of the most frequent diseases and their prevalence continues to increase. They develop as a consequence of dysregulation of the immune system, especially when there is failure in the equilibrium of the response of TH1/TH2 lymphocytes towards TH2. New therapeutic approaches in the treatment of immunopathological conditions (e.g. allergic or oncologic) are directed to restoring the equilibrium among different T lymphocyte subpopulations. Based on in vitro experiments, and also on animal and human clinical studies, there is much evidence for the importance of β-glucans in the treatment and also prevention of allergic diseases; this opens new perspectives on the use of this widespread and popular group of natural substances.
    MeSH term(s) Animals ; Humans ; Hypersensitivity/drug therapy ; Hypersensitivity/immunology ; beta-Glucans/immunology ; beta-Glucans/pharmacology
    Chemical Substances beta-Glucans
    Language English
    Publishing date 2014-03
    Publishing country Spain
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 193144-1
    ISSN 1578-1267 ; 0301-0546
    ISSN (online) 1578-1267
    ISSN 0301-0546
    DOI 10.1016/j.aller.2012.08.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Anti-allergic effect of Pleuran (β-glucan from Pleurotus ostreatus) in children with recurrent respiratory tract infections.

    Jesenak, Milos / Hrubisko, Martin / Majtan, Juraj / Rennerova, Zuzana / Banovcin, Peter

    Phytotherapy research : PTR

    2014  Volume 28, Issue 3, Page(s) 471–474

    Abstract: Recurrent respiratory tract infections (RRTIs) present a very important problem in paediatric praxis. As true immunodeficiencies are rare, one of the most important factors assumed to contribute to increased respiratory morbidity is atopy. Several ... ...

    Abstract Recurrent respiratory tract infections (RRTIs) present a very important problem in paediatric praxis. As true immunodeficiencies are rare, one of the most important factors assumed to contribute to increased respiratory morbidity is atopy. Several preparations of natural origin have been used for the prevention of RRTIs, and some of the most effective immunomodulators are biologically active polysaccharides - e.g. ß-glucans. In our randomised, double-blind, placebo-controlled study, we investigated the prevalence of atopy in a group of children with RRTIs and the potential anti-allergic effect of pleuran (ß-glucan isolated from Pleurotus ostreatus) on basic laboratory markers of allergic inflammation. We confirmed that atopy may be an important factor contributing to the increased respiratory morbidity in children with RRTIs. The active treatment with pleuran resulted in a significant reduction of peripheral blood eosinophilia and stabilised the levels of total IgE in serum. This was more evident in atopic subjects. Pleuran showed a potential anti-allergic effect. This previously non-described effect could expand the application of this natural immunomodulator also as a complementary adjuvant therapy in allergic patients.
    MeSH term(s) Anti-Allergic Agents/therapeutic use ; Child ; Child, Preschool ; Double-Blind Method ; Eosinophilia/drug therapy ; Female ; Humans ; Hypersensitivity, Immediate/drug therapy ; Immunoglobulin E/blood ; Male ; Pleurotus/chemistry ; Respiratory Tract Infections/drug therapy ; beta-Glucans/therapeutic use
    Chemical Substances Anti-Allergic Agents ; beta-Glucans ; Immunoglobulin E (37341-29-0)
    Language English
    Publishing date 2014-03
    Publishing country England
    Document type Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 639136-9
    ISSN 1099-1573 ; 0951-418X
    ISSN (online) 1099-1573
    ISSN 0951-418X
    DOI 10.1002/ptr.5020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Immune Parameters and COVID-19 Infection – Associations With Clinical Severity and Disease Prognosis

    Jesenak, Milos / Brndiarova, Miroslava / Urbancikova, Ingrid / Rennerova, Zuzana / Vojtkova, Jarmila / Bobcakova, Anna / Ostro, Robert / Banovcin, Peter

    Frontiers in Cellular and Infection Microbiology

    2020  Volume 10

    Keywords Immunology ; Microbiology (medical) ; Microbiology ; Infectious Diseases ; covid19
    Publisher Frontiers Media SA
    Publishing country ch
    Document type Article ; Online
    ZDB-ID 2619676-1
    ISSN 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2020.00364
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Anti‐allergic Effect of Pleuran (β‐glucan from Pleurotus ostreatus) in Children with Recurrent Respiratory Tract Infections

    Jesenak, Milos / Hrubisko, Martin / Majtan, Juraj / Rennerova, Zuzana / Banovcin, Peter

    Phytotherapy research. 2014 Mar., v. 28, no. 3

    2014  

    Abstract: Recurrent respiratory tract infections (RRTIs) present a very important problem in paediatric praxis. As true immunodeficiencies are rare, one of the most important factors assumed to contribute to increased respiratory morbidity is atopy. Several ... ...

    Abstract Recurrent respiratory tract infections (RRTIs) present a very important problem in paediatric praxis. As true immunodeficiencies are rare, one of the most important factors assumed to contribute to increased respiratory morbidity is atopy. Several preparations of natural origin have been used for the prevention of RRTIs, and some of the most effective immunomodulators are biologically active polysaccharides – e.g. ß‐glucans. In our randomised, double‐blind, placebo‐controlled study, we investigated the prevalence of atopy in a group of children with RRTIs and the potential anti‐allergic effect of pleuran (ß‐glucan isolated from Pleurotus ostreatus) on basic laboratory markers of allergic inflammation. We confirmed that atopy may be an important factor contributing to the increased respiratory morbidity in children with RRTIs. The active treatment with pleuran resulted in a significant reduction of peripheral blood eosinophilia and stabilised the levels of total IgE in serum. This was more evident in atopic subjects. Pleuran showed a potential anti‐allergic effect. This previously non‐described effect could expand the application of this natural immunomodulator also as a complementary adjuvant therapy in allergic patients. Copyright © 2013 John Wiley & Sons, Ltd.
    Keywords Pleurotus ostreatus ; atopy ; beta-glucans ; blood ; children ; eosinophilia ; immunoglobulin E ; immunomodulators ; inflammation ; morbidity ; patients ; respiratory system ; therapeutics
    Language English
    Dates of publication 2014-03
    Size p. 471-474.
    Document type Article
    ZDB-ID 639136-9
    ISSN 1099-1573 ; 0951-418X
    ISSN (online) 1099-1573
    ISSN 0951-418X
    DOI 10.1002/ptr.5020
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Is asthma caused by atopy (positive skin prick tests)? Epidemiologic evidence suggests a negative answer.

    Ronchetti, Roberto / Jesenak, Milos / Ronchetti, Francesco / Rennerova, Zuzana

    Inflammation & allergy drug targets

    2009  Volume 9, Issue 2, Page(s) 91–96

    Abstract: In this paper we shortly discuss epidemiological data on the relationship between asthma and atopy according to recent personal and literature epidemiological publications. The coexistence in several subjects of asthma (and of other "atopic" diseases) ... ...

    Abstract In this paper we shortly discuss epidemiological data on the relationship between asthma and atopy according to recent personal and literature epidemiological publications. The coexistence in several subjects of asthma (and of other "atopic" diseases) and IgE hyperproduction generated the dogma that these two biological conditions are mainly genetic in origin and are linked by a strong casual relationship. In the last decades atopy increased at 5-10 % annual rate and at present atopy prevalence, although variable in different countries, reaches somewhere the prevalence of more than 60 %. Similarly, asthma prevalence increased in the last decades, especially so in western and English speaking countries, reaching in certain countries prevalence values higher than 40 %. For these reasons, although certainly dependent on a genetic predisposition, atopy and asthma can nowadays be considered to be largely determined by environmental factors. Moreover, the analysis of epidemiological data derived from studies conducted worldwide, showed that the prevalence of the two conditions were clearly not correlated so that in certain countries with a 50-60% prevalence of atopy asthma prevalence is lower than 2-6 %, while in other countries asthma prevalence is double than atopy prevalence. Further, in countries with high atopy, the prevalence this conditions is high both in asthmatics and in normal subjects and in the places where asthma prevalence reaches high levels this condition is high both in atopic and non atopic people. In conclusion, epidemiological data show that environmental factors affecting asthma prevalence are different from those affecting atopy prevalence and that subjects bearing one of the two conditions don't show any preferential tendency to develop the other one. From aetiological and pathogenetic point of view asthma and atopy appear to be independent conditions We therefore believe that the association between asthma and atopy demonstrated in almost all the "population studies" (atopy prevalence has always been found 20-30 % higher in asthmatics than in non asthmatic subjects) doesn't prove the existence of a "causal" relationship between the two conditions: this could be a kind of association without causative meaning as is the association between blond hair and blue eyes which in no way can be considered a prove that one of the two conditions is the cause of the other.
    MeSH term(s) Allergens/immunology ; Asthma/epidemiology ; Asthma/immunology ; Histamine/immunology ; Humans ; Hypersensitivity, Immediate/epidemiology ; Hypersensitivity, Immediate/genetics ; Hypersensitivity, Immediate/immunology ; Immunoglobulin E/immunology ; Prevalence ; Skin Tests
    Chemical Substances Allergens ; Immunoglobulin E (37341-29-0) ; Histamine (820484N8I3)
    Language English
    Publishing date 2009-11-05
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2215862-5
    ISSN 2212-4055 ; 1871-5281
    ISSN (online) 2212-4055
    ISSN 1871-5281
    DOI 10.2174/187152810791292791
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Immunomodulatory effect of pleuran (β-glucan from Pleurotus ostreatus) in children with recurrent respiratory tract infections.

    Jesenak, Milos / Majtan, Juraj / Rennerova, Zuzana / Kyselovic, Jan / Banovcin, Peter / Hrubisko, Martin

    International immunopharmacology

    2013  Volume 15, Issue 2, Page(s) 395–399

    Abstract: Objectives: Recurrent respiratory tract infections (RRTIs) represent a very important problem in daily clinical practice because of their significant contribution to morbidity in children. Several natural nutritional supplements have been used in the ... ...

    Abstract Objectives: Recurrent respiratory tract infections (RRTIs) represent a very important problem in daily clinical practice because of their significant contribution to morbidity in children. Several natural nutritional supplements have been used in the prevention of RRTIs, but the clinical efficacy of only a few preparations is supported by scientific evidence.
    Materials and methods: In a double-blind, placebo-controlled, randomised, multicentre study, we have observed a group of 175 children (aged 5.65 ± 2.39 years) with more than 5 respiratory infections that occurred during the 12 months prior to the beginning of the study. Children were randomised into an active group, treated with Imunoglukan P4H® syrup (with pleuran-β-glucan from Pleurotus ostreatus and vitamin C), or a placebo group (vitamin C only). During the 3 visits, within a 12-month period, questionnaires were completed, and blood samples were examined for immune parameters.
    Results: In the active group, 36% of the children did not suffer from any respiratory infections throughout the treatment, compared to 21% in the placebo group (p<0.05). Imunoglukan P4H® also significantly decreased the frequency of flu and flu-like disease and the number of lower respiratory tract infections. Imunoglukan P4H® treatment resulted in a statistically significant modulation of humoral and cellular immunity.
    Conclusions: Results from this study demonstrate that Imunoglukan P4H® is effective in the prevention of RRTIs in children. Furthermore, our results also revealed complex immunomodulatory activity of this product. This is the first double-blind, placebo-controlled study in children with RRTIs that has addressed the preventive effects of pleuran on morbidity caused by respiratory infections.
    MeSH term(s) Adjuvants, Immunologic/administration & dosage ; Adjuvants, Immunologic/adverse effects ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Humans ; Immunoglobulin G/blood ; Immunomodulation ; Immunophenotyping ; Male ; Pleurotus ; Recurrence ; Respiratory Tract Infections/drug therapy ; Respiratory Tract Infections/immunology ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology ; beta-Glucans/administration & dosage ; beta-Glucans/adverse effects ; beta-Glucans/therapeutic use
    Chemical Substances Adjuvants, Immunologic ; Immunoglobulin G ; beta-Glucans
    Language English
    Publishing date 2013-02
    Publishing country Netherlands
    Document type Journal Article ; Multicenter Study ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 2043785-7
    ISSN 1878-1705 ; 1567-5769
    ISSN (online) 1878-1705
    ISSN 1567-5769
    DOI 10.1016/j.intimp.2012.11.020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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