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  1. Article ; Online: Saliva "Treat-and-Heat" Triplex Reverse Transcription Loop-Mediated Isothermal Amplification Assay for SARS-CoV-2.

    Reolo, Marie J Y / Eleazar, Carlos S R / Sonio, Joseph P / Solon, Ryonne T / Villamor, Janika L B / Loedin, Alexandra K / Moore, Keith J M

    Journal of biomolecular techniques : JBT

    2022  Volume 32, Issue 3, Page(s) 186–198

    Abstract: The demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) molecular diagnostics that are faster, cheaper, and simpler to run than nasopharyngeal-based reverse transcription quantitative PCR (RT-qPCR) tests remains unmet in many parts of ...

    Abstract The demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) molecular diagnostics that are faster, cheaper, and simpler to run than nasopharyngeal-based reverse transcription quantitative PCR (RT-qPCR) tests remains unmet in many parts of the world. In the Philippines, geographical and economic access to quality diagnostic testing remains out of reach for many communities. We describe the preclinical development of a fluorescence-based reverse transcription loop-mediated isothermal amplification test that uses drooled saliva as the biospecimen. Six treat-and-heat ("direct") procedures that inactivate the virus and release the target RNA were compared. Using duplexed As1e and E1 primers, protocols derived from Ben-Assa et al. (2020) using proteinase K or from Rabe and Cepko (2020) using TCEP (Tris(2-carboxyethyl)phosphine hydrochloride)/EDTA provided reliable RNA amplification. The TCEP/EDTA-based method in particular showed improvement in robustness in duplex vs. singleplex format. Inclusion of human
    MeSH term(s) COVID-19 ; Hot Temperature ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; RNA, Viral/genetics ; Reverse Transcription ; SARS-CoV-2 ; Saliva ; Sensitivity and Specificity
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2022-01-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2116011-9
    ISSN 1943-4731 ; 1943-4731
    ISSN (online) 1943-4731
    ISSN 1943-4731
    DOI 10.7171/jbt.21-3203-014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: CD38 marks the exhausted CD8

    Reolo, Marie J Y / Otsuka, Masayuki / Seow, Justine Jia Wen / Lee, Joycelyn / Lee, Yun Hua / Nguyen, Phuong H D / Lim, Chun Jye / Wasser, Martin / Chua, Camillus / Lim, Tony K H / Leow, Wei Qiang / Chung, Alexander / Goh, Brian K P / Chow, Pierce K H / DasGupta, Ramanuj / Yeong, Joe Poh Sheng / Chew, Valerie

    Frontiers in immunology

    2023  Volume 14, Page(s) 1182016

    Abstract: Introduction: Despite recent advances in immunotherapy for hepatocellular carcinoma (HCC), the overall modest response rate underscores the need for a better understanding of the tumor microenvironment (TME) of HCC. We have previously shown that CD38 is ...

    Abstract Introduction: Despite recent advances in immunotherapy for hepatocellular carcinoma (HCC), the overall modest response rate underscores the need for a better understanding of the tumor microenvironment (TME) of HCC. We have previously shown that CD38 is widely expressed on tumor-infiltrating leukocytes (TILs), predominantly on CD3
    Methods: In this current study, we used cytometry time-of-flight (CyTOF), bulk RNA sequencing on sorted T cells, and single-cell RNA (scRNA) sequencing to interrogate expression of CD38 and its correlation with T cell exhaustion in HCC samples. We also employed multiplex immunohistochemistry (mIHC) for validating our findings.
    Results: From CyTOF analysis, we compared the immune composition of CD38-expressing leukocytes in TILs, non-tumor tissue-infiltrating leukocytes (NIL), and peripheral blood mononuclear cells (PBMC). We identified CD8
    Conclusion: Taken together, the concurrent expression of CD38 with exhaustion markers on CD8
    MeSH term(s) Humans ; Carcinoma, Hepatocellular/pathology ; Liver Neoplasms/pathology ; CD8-Positive T-Lymphocytes ; Leukocytes, Mononuclear/metabolism ; Programmed Cell Death 1 Receptor/metabolism ; CTLA-4 Antigen/metabolism ; Memory T Cells ; CD3 Complex/metabolism ; Tumor Microenvironment
    Chemical Substances Programmed Cell Death 1 Receptor ; CTLA-4 Antigen ; CD3 Complex
    Language English
    Publishing date 2023-06-12
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1182016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Sphingosine 1-Phosphate Receptor 2 Induces Otoprotective Responses to Cisplatin Treatment.

    Wang, Wei / Shanmugam, Muthu K / Xiang, Ping / Yam, Ting Yu Amelia / Kumar, Vineet / Chew, Wee Siong / Chang, Jing Kai / Ali, Muhammad Zulfaqar Bin / Reolo, Marie J Y / Peh, Yee Xin / Karim, Siti Nasuha Binte Abdul / Tan, Andrew Y Y / Sanda, Takaomi / Sethi, Gautam / Herr, Deron R

    Cancers

    2020  Volume 12, Issue 1

    Abstract: Ototoxicity is a major adverse effect of platinum-based chemotherapeutics and currently, there remains a lack of United States Food and Drug Administration-approved therapies to prevent or treat this problem. In our study, we examined the role of the ... ...

    Abstract Ototoxicity is a major adverse effect of platinum-based chemotherapeutics and currently, there remains a lack of United States Food and Drug Administration-approved therapies to prevent or treat this problem. In our study, we examined the role of the sphingosine 1-phosphate receptor 2 (S1P
    Language English
    Publishing date 2020-01-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers12010211
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Sphingosine 1-phosphate receptor 2 (S1P2) attenuates reactive oxygen species formation and inhibits cell death: implications for otoprotective therapy.

    Herr, Deron R / Reolo, Marie J Y / Peh, Yee Xin / Wang, Wei / Lee, Chang-Wook / Rivera, Rich / Paterson, Ian C / Chun, Jerold

    Scientific reports

    2016  Volume 6, Page(s) 24541

    Abstract: Ototoxic drugs, such as platinum-based chemotherapeutics, often lead to permanent hearing loss through apoptosis of neuroepithelial hair cells and afferent neurons of the cochlea. There is no approved therapy for preventing or reversing this process. Our ...

    Abstract Ototoxic drugs, such as platinum-based chemotherapeutics, often lead to permanent hearing loss through apoptosis of neuroepithelial hair cells and afferent neurons of the cochlea. There is no approved therapy for preventing or reversing this process. Our previous studies identified a G protein-coupled receptor (GPCR), S1P2, as a potential mediator of otoprotection. We therefore sought to identify a pharmacological approach to prevent cochlear degeneration via activation of S1P2. The cochleae of S1pr2(-/-) knockout mice were evaluated for accumulation of reactive oxygen species (ROS) with a nitro blue tetrazolium (NBT) assay. This showed that loss of S1P2 results in accumulation of ROS that precedes progressive cochlear degeneration as previously reported. These findings were supported by in vitro cell-based assays to evaluate cell viability, induction of apoptosis, and accumulation of ROS following activation of S1P2 in the presence of cisplatin. We show for the first time, that activation of S1P2 with a selective receptor agonist increases cell viability and reduces cisplatin-mediated cell death by reducing ROS. Cumulatively, these results suggest that S1P2 may serve as a therapeutic target for attenuating cisplatin-mediated ototoxicity.
    MeSH term(s) Animals ; Cell Death/genetics ; Cell Survival/drug effects ; Cochlea/metabolism ; Cochlea/pathology ; Mice ; Mice, Knockout ; Reactive Oxygen Species/metabolism ; Receptors, Lysosphingolipid/agonists ; Receptors, Lysosphingolipid/genetics ; Receptors, Lysosphingolipid/metabolism ; Signal Transduction/drug effects
    Chemical Substances Reactive Oxygen Species ; Receptors, Lysosphingolipid
    Language English
    Publishing date 2016-04-15
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/srep24541
    Database MEDical Literature Analysis and Retrieval System OnLINE

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