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  1. Article ; Online: Prevalence of HTLV-I Infection and Its Association with Tuberculosis among Patients at an Urban Clinic in Haiti.

    Walsh, Kathleen F / Lee, Myung Hee / Brejt, Josef A / Reust, Mary J / Juste, Marc Jean / Hilaire, Genevieve / Pape, Jean William / Koenig, Serena / Dupnik, Kathryn

    The American journal of tropical medicine and hygiene

    2022  

    Abstract: This retrospective case-control study examined the prevalence of HTLV-I and its association with tuberculosis among urban clinic patients in Haiti. Prevalence of HTLV-I among tuberculosis cases was 2.1% and among controls was 2.4%. Prevalence of HLTV-I ... ...

    Abstract This retrospective case-control study examined the prevalence of HTLV-I and its association with tuberculosis among urban clinic patients in Haiti. Prevalence of HTLV-I among tuberculosis cases was 2.1% and among controls was 2.4%. Prevalence of HLTV-I was higher in females than males (odds ratio [OR] 2.45, P = 0.020). HTLV-I prevalence in those ≥ 50 years was 8.4% compared with 1.3% in those < 50 (OR 6.74, P < 0.001). We found no association between HTLV-I and tuberculosis in this population.
    Language English
    Publishing date 2022-03-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2942-7
    ISSN 1476-1645 ; 0002-9637
    ISSN (online) 1476-1645
    ISSN 0002-9637
    DOI 10.4269/ajtmh.21-0702
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Dried Blood Spot RNA Transcriptomes Correlate with Transcriptomes Derived from Whole Blood RNA.

    Reust, Mary J / Lee, Myung Hee / Xiang, Jenny / Zhang, Wei / Xu, Dong / Batson, Tatiana / Zhang, Tuo / Downs, Jennifer A / Dupnik, Kathryn M

    The American journal of tropical medicine and hygiene

    2018  Volume 98, Issue 5, Page(s) 1541–1546

    Abstract: Obtaining RNA from clinical samples collected in resource-limited settings can be costly and challenging. The goals of this study were to 1) optimize messenger RNA extraction from dried blood spots (DBS) and 2) determine how transcriptomes generated from ...

    Abstract Obtaining RNA from clinical samples collected in resource-limited settings can be costly and challenging. The goals of this study were to 1) optimize messenger RNA extraction from dried blood spots (DBS) and 2) determine how transcriptomes generated from DBS RNA compared with RNA isolated from blood collected in Tempus tubes. We studied paired samples collected from eight adults in rural Tanzania. Venous blood was collected on Whatman 903 Protein Saver cards and in tubes with RNA preservation solution. Our optimal DBS RNA extraction used 8 × 3-mm DBS punches as the starting material, bead beater disruption at maximum speed for 60 seconds, extraction with Illustra RNAspin Mini RNA Isolation kit, and purification with Zymo RNA Concentrator kit. Spearman correlations of normalized gene counts in DBS versus whole blood ranged from 0.887 to 0.941. Bland-Altman plots did not show a trend toward over- or under-counting at any gene size. We report a method to obtain sufficient RNA from DBS to generate a transcriptome. The DBS transcriptome gene counts correlated well with whole blood transcriptome gene counts. Dried blood spots for transcriptome studies could be an option when field conditions preclude appropriate collection, storage, or transport of whole blood for RNA studies.
    MeSH term(s) Adult ; Dried Blood Spot Testing/methods ; Female ; Humans ; Male ; RNA/blood ; RNA/chemistry ; Tanzania ; Transcriptome ; Young Adult
    Chemical Substances RNA (63231-63-0)
    Language English
    Publishing date 2018-03-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2942-7
    ISSN 1476-1645 ; 0002-9637
    ISSN (online) 1476-1645
    ISSN 0002-9637
    DOI 10.4269/ajtmh.17-0653
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Monobenzyl ether of hydroquinone and 4-tertiary butyl phenol activate markedly different physiological responses in melanocytes: relevance to skin depigmentation.

    Hariharan, Vidhya / Klarquist, Jared / Reust, Mary J / Koshoffer, Amy / McKee, Mark D / Boissy, Raymond E / Le Poole, I Caroline

    The Journal of investigative dermatology

    2010  Volume 130, Issue 1, Page(s) 211–220

    Abstract: Monobenzyl ether of hydroquinone (MBEH) is a Food and Drug Administration approved drug used for depigmentation therapy of advanced vitiligo. Here, the working mechanism of MBEH is explored in comparison to 4-tertiary butyl phenol (4-TBP), a known ... ...

    Abstract Monobenzyl ether of hydroquinone (MBEH) is a Food and Drug Administration approved drug used for depigmentation therapy of advanced vitiligo. Here, the working mechanism of MBEH is explored in comparison to 4-tertiary butyl phenol (4-TBP), a known causative agent for occupational vitiligo mediating apoptotic melanocytic death. Cytotoxic experiments reveal that similar to 4-TBP, MBEH induces specific melanocyte death. To compare death pathways initiated by 4-TBP and MBEH, classical apoptotic hallmarks were evaluated in treated melanocytes. MBEH induced cell death without activating the caspase cascade or DNA fragmentation, showing that the death pathway is non-apoptotic. Release of High Mobility Group Box-1 protein by MBEH-treated melanocytes and ultrastructural features further confirmed a necrotic death pathway mediated by MBEH. A negative correlation between MBEH-induced cell death and cellular melanin content supports a cytoprotective role for melanin. Moreover, MBEH exposure upregulated the levels of melanogenic enzymes in cultured melanocytes and skin explants, whereas 4-TBP reduced the expression of the same. In summary, exposure to MBEH or 4-TBP has profoundly different consequences for melanocyte physiology and activates different death pathways. As the mode of cell death defines the nature of the immune response that follows, these findings help to explain the relative efficacy of these agents in mediating depigmentation.
    MeSH term(s) Annexin A5/metabolism ; Apoptosis/drug effects ; Biomarkers/metabolism ; Caspase 3/metabolism ; Cell Survival/drug effects ; Cells, Cultured ; DNA Fragmentation/drug effects ; Fibroblasts/cytology ; Fibroblasts/drug effects ; Humans ; Hydroquinones/pharmacology ; Hydroquinones/toxicity ; Keratinocytes/cytology ; Keratinocytes/drug effects ; Melanocytes/cytology ; Melanocytes/drug effects ; Necrosis ; Organ Culture Techniques ; Phenols/pharmacology ; Phenols/toxicity ; Poly(ADP-ribose) Polymerases/metabolism ; Skin/cytology ; Skin Pigmentation/drug effects ; Vitiligo/drug therapy ; Vitiligo/pathology
    Chemical Substances Annexin A5 ; Biomarkers ; Hydroquinones ; Phenols ; monobenzone (9L2KA76MG5) ; Poly(ADP-ribose) Polymerases (EC 2.4.2.30) ; CASP3 protein, human (EC 3.4.22.-) ; Caspase 3 (EC 3.4.22.-) ; butylphen (O81VMW36CV)
    Language English
    Publishing date 2010-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 80136-7
    ISSN 1523-1747 ; 0022-202X
    ISSN (online) 1523-1747
    ISSN 0022-202X
    DOI 10.1038/jid.2009.214
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Melanoma-associated antigen expression in lymphangioleiomyomatosis renders tumor cells susceptible to cytotoxic T cells.

    Klarquist, Jared / Barfuss, Allison / Kandala, Sridhar / Reust, Mary J / Braun, Ruedi K / Hu, Jennifer / Dilling, Daniel F / McKee, Mark D / Boissy, Raymond E / Love, Robert B / Nishimura, Michael I / Le Poole, I Caroline

    The American journal of pathology

    2009  Volume 175, Issue 6, Page(s) 2463–2472

    Abstract: The antibody HMB45 is used to diagnose lymphangioleiomyomatosis, a hyperproliferative disorder of lung smooth muscle cells with mutations in both alleles of either TSC1 or TSC2. A subset of these tumor cells expresses the melanoma-associated antigens ... ...

    Abstract The antibody HMB45 is used to diagnose lymphangioleiomyomatosis, a hyperproliferative disorder of lung smooth muscle cells with mutations in both alleles of either TSC1 or TSC2. A subset of these tumor cells expresses the melanoma-associated antigens gp100 and melanoma antigen recognized by T cells (MART-1). To explore the feasibility of targeting tumors in lymphangioleiomyomatosis by melanoma immunotherapy, we therefore assessed melanoma target antigen expression and existing immune infiltration of affected tissue compared with normal lung and melanoma as well as the susceptibility of cultured lymphangioleiomyomatosis cells to melanoma reactive cytotoxic T lymphocytes in vitro. Tumors expressed tyrosinase-related proteins 1 and 2 but not tyrosinase, in addition to gp100 and MART-1, and were densely infiltrated by macrophages, but not dendritic cells or T cell subsets. Although CD8(+) lymphocytes were sparse compared with melanoma, cells cultured from lymphangioleiomyomatosis tissue were susceptible to cytotoxic, gp100 reactive, and major histocompatibility complex class I restricted CD8(+) T cells in functional assays. Responder T cells selectively clustered and secreted interferon-gamma in response to HLA-matched melanocytes and cultured lymphangioleiomyomatosis cells. This reactivity exceeded that based on detectable gp100 expression; thus, tumor cells in lymphangioleiomyomatosis may process melanosomal antigens different from melanocytic cells. Therefore, boosting immune responses to gp100 in lymphangioleiomyomatosis may offer a highly desirable treatment option for this condition.
    MeSH term(s) Antigens, Neoplasm/immunology ; Antigens, Neoplasm/metabolism ; Cell Separation ; Cytotoxicity, Immunologic ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Humans ; Immunohistochemistry ; Immunotherapy/methods ; Intramolecular Oxidoreductases/immunology ; Intramolecular Oxidoreductases/metabolism ; Lymphangioleiomyomatosis/immunology ; Lymphangioleiomyomatosis/metabolism ; Lymphocytes, Tumor-Infiltrating/immunology ; MART-1 Antigen ; Melanoma/immunology ; Membrane Glycoproteins/immunology ; Membrane Glycoproteins/metabolism ; Microscopy, Electron, Transmission ; Neoplasm Proteins/immunology ; Neoplasm Proteins/metabolism ; Oxidoreductases/immunology ; Oxidoreductases/metabolism ; Skin Neoplasms/immunology ; T-Lymphocytes, Cytotoxic/immunology ; gp100 Melanoma Antigen
    Chemical Substances Antigens, Neoplasm ; MART-1 Antigen ; MLANA protein, human ; Membrane Glycoproteins ; Neoplasm Proteins ; PMEL protein, human ; gp100 Melanoma Antigen ; Oxidoreductases (EC 1.-) ; tyrosinase-related protein-1 (EC 1.14.18.-) ; Intramolecular Oxidoreductases (EC 5.3.-) ; dopachrome isomerase (EC 5.3.3.12)
    Language English
    Publishing date 2009-11-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2943-9
    ISSN 1525-2191 ; 0002-9440
    ISSN (online) 1525-2191
    ISSN 0002-9440
    DOI 10.2353/ajpath.2009.090525
    Database MEDical Literature Analysis and Retrieval System OnLINE

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