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  1. Article ; Online: Detection and quantification of natural

    Reyes, Jerica Isabel L / Suzuki, Takahiro / Suzuki, Yasutsugu / Watanabe, Kozo

    Frontiers in cellular and infection microbiology

    2024  Volume 14, Page(s) 1360438

    Abstract: Background: The Philippines bears health and economic burden caused by high dengue cases annually. Presently, the Philippines still lack an effective and sustainable vector management. The use of : Methods: Ae. aegypti: Results: Wolbachia: ... ...

    Abstract Background: The Philippines bears health and economic burden caused by high dengue cases annually. Presently, the Philippines still lack an effective and sustainable vector management. The use of
    Methods: Ae. aegypti
    Results: Wolbachia
    Conclusion: Location-specific primers improved detection of natural
    MeSH term(s) Animals ; Humans ; Aedes/microbiology ; Wolbachia/genetics ; Philippines ; RNA, Ribosomal, 16S/genetics ; Mosquito Vectors ; Phylogeny
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-03-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2024.1360438
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: In vitro characterization of cell-fusing agent virus DNA forms in Aedes aegypti mosquitoes.

    Uddin, Mohammad Mosleh / Suzuki, Yasutsugu / Reyes, Jerica Isabel L / Watanabe, Kozo

    Virology

    2024  Volume 591, Page(s) 109982

    Abstract: How non-retroviral endogenous viral elements (EVEs) are established is a long-standing question. Viral DNA (vDNA) forms of RNA viruses are likely to be EVE precursors. Cell-fusing agent virus (CFAV) is a major insect-specific virus (ISV) in the Aedes ... ...

    Abstract How non-retroviral endogenous viral elements (EVEs) are established is a long-standing question. Viral DNA (vDNA) forms of RNA viruses are likely to be EVE precursors. Cell-fusing agent virus (CFAV) is a major insect-specific virus (ISV) in the Aedes aegypti mosquitoes and one of the few existing non-retroviral RNA viruses found as EVEs. We characterized CFAV-derived vDNA in the cell line to understand the mechanism of why current viruses are rarely endogenized. vDNA production was affected by cell culture media independent of CFAV replication. vDNAs that correspond to different regions covering the entire viral genome were detected, implying multiple initiation sites exist. A considerable proportion of vDNAs corresponded to ssDNA. Higher vDNA copies were detected in the cytoplasm than the nucleus. Our findings provide valuable insights into the intracellular characteristics of ISV-derived vDNAs, which will aid in understanding the underlying mechanisms of non-retroviral EVE formation.
    MeSH term(s) Animals ; Aedes ; DNA, Viral/genetics ; Cell Line ; Virus Replication ; RNA Viruses/genetics ; DNA Viruses/genetics
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2024-01-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/j.virol.2024.109982
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Genome-wide detection of

    Muharromah, Atikah Fitria / Reyes, Jerica Isabel L / Kagia, Ngure / Watanabe, Kozo

    Frontiers in cellular and infection microbiology

    2023  Volume 13, Page(s) 1252656

    Abstract: Background: Wolbachia: Methods: A total of 217 individuals : Results: From 146,239,637 sequence reads obtained, 26,299 and 43,778 reads were mapped across the entire : Conclusions: Taken together, our results demonstrate the feasibility of ... ...

    Abstract Background: Wolbachia
    Methods: A total of 217 individuals
    Results: From 146,239,637 sequence reads obtained, 26,299 and 43,778 reads were mapped across the entire
    Conclusions: Taken together, our results demonstrate the feasibility of ddRAD-Seq-based
    MeSH term(s) Humans ; Animals ; Male ; Female ; Aedes/microbiology ; Wolbachia/genetics ; RNA, Ribosomal, 16S/genetics ; Philippines ; Ribosomal Proteins
    Chemical Substances RNA, Ribosomal, 16S ; Ribosomal Proteins
    Language English
    Publishing date 2023-12-14
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2023.1252656
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Acute oral toxicity assessment of ethanolic extracts of Antidesma bunius (L.) Spreng fruits in mice

    Muñoz, Maria Nilda M. / Alvarado, Urdujah G. / Reyes, Jerica Isabel L. / Watanabe, Kozo

    Toxicology reports. 2021, v. 8

    2021  

    Abstract: The aim of the study is to assess the acute oral toxicity of ethanolic extracts of Antidesma bunius (L.) Spreng [Bignay] in ICR mice in accordance to OECD guideline 423. Single doses of Bignay fruit extracts ranging from 500 mg/kg to 2000 mg/kg, as well ... ...

    Abstract The aim of the study is to assess the acute oral toxicity of ethanolic extracts of Antidesma bunius (L.) Spreng [Bignay] in ICR mice in accordance to OECD guideline 423. Single doses of Bignay fruit extracts ranging from 500 mg/kg to 2000 mg/kg, as well as a vehicle control, were given orally and monitored for 14 days. We observed that there is no mortality or adverse effects after treatment of mice indicating that Bignay extracts are safe for use in laboratory animals. Behavioral, respiratory, and neurologic changes, as well as changes in body weight, food and water consumption, did not occur during the experimental period. Hematological- (total-red and -white blood cells) and biochemical-profile analysis (alanine transaminase, blood urea nitrogen, and creatinine) remained within normal concentrations in treated mice regardless of sex. The morphology of visceral organs in all treated mice was typical in appearance when stained with H&E. It is assumed that the LD₅₀ is greater than 2000 mg/kg and there is no mortality at the maximum dose used (2000 mg/kg). We have also determined the active components of dried Bignay by headspace GC–MS and by phytochemical analysis of ethanolic Bignay extracts. We have identified 16 compounds by GC–MS and 10 secondary metabolites. In this study, the assessment of extracted Antidesma bunius (L.) Spreng fruits [Bignay] on the value of safety measures in mice is described.
    Keywords Antidesma bunius ; acute oral toxicity ; alanine transaminase ; body weight ; creatinine ; fruits ; guidelines ; headspace analysis ; mortality ; phytochemicals ; secondary metabolites ; toxicology ; urea nitrogen
    Language English
    Size p. 1289-1299.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2805786-7
    ISSN 2214-7500
    ISSN 2214-7500
    DOI 10.1016/j.toxrep.2021.06.010
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: No detectable fitness cost of infection by cell-fusing agent virus in

    Suzuki, Yasutsugu / Suzuki, Takahiro / Miura, Fuminari / Reyes, Jerica Isabel L / Asin, Irish Coleen A / Mitsunari, Wataru / Uddin, Mohammad Mosleh / Sekii, Yu / Watanabe, Kozo

    Royal Society open science

    2024  Volume 11, Issue 1, Page(s) 231373

    Abstract: ... ...

    Abstract Aedes
    Language English
    Publishing date 2024-01-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2787755-3
    ISSN 2054-5703
    ISSN 2054-5703
    DOI 10.1098/rsos.231373
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Intracellular Interactions Between Arboviruses and

    Reyes, Jerica Isabel L / Suzuki, Yasutsugu / Carvajal, Thaddeus / Muñoz, Maria Nilda M / Watanabe, Kozo

    Frontiers in cellular and infection microbiology

    2021  Volume 11, Page(s) 690087

    Abstract: ... Aedes ... ...

    Abstract Aedes aegypti
    MeSH term(s) Aedes ; Animals ; Arboviruses ; Humans ; Mosquito Vectors ; Wolbachia ; Zika Virus ; Zika Virus Infection
    Language English
    Publishing date 2021-06-23
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2021.690087
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Acute oral toxicity assessment of ethanolic extracts of

    Muñoz, Maria Nilda M / Alvarado, Urdujah G / Reyes, Jerica Isabel L / Watanabe, Kozo

    Toxicology reports

    2021  Volume 8, Page(s) 1289–1299

    Abstract: The aim of the study is to assess the acute oral toxicity of ethanolic extracts ... ...

    Abstract The aim of the study is to assess the acute oral toxicity of ethanolic extracts of
    Language English
    Publishing date 2021-06-17
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 2805786-7
    ISSN 2214-7500 ; 2214-7500
    ISSN (online) 2214-7500
    ISSN 2214-7500
    DOI 10.1016/j.toxrep.2021.06.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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