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  1. Article ; Online: GNAS AS2 methylation status enables mechanism-based categorization of pseudohypoparathyroidism type 1B.

    Iwasaki, Yorihiro / Reyes, Monica / Jüppner, Harald / Bastepe, Murat

    JCI insight

    2024  Volume 9, Issue 5

    Abstract: Pseudohypoparathyroidism type 1B (PHP1B) results from aberrant genomic imprinting at the GNAS gene. Defining the underlying genetic cause in new patients is challenging because various genetic alterations (e.g., deletions, insertions) within the GNAS ... ...

    Abstract Pseudohypoparathyroidism type 1B (PHP1B) results from aberrant genomic imprinting at the GNAS gene. Defining the underlying genetic cause in new patients is challenging because various genetic alterations (e.g., deletions, insertions) within the GNAS genomic region, including the neighboring STX16 gene, can cause PHP1B, and the genotype-epigenotype correlation has not been clearly established. Here, by analyzing patients with PHP1B with a wide variety of genotypes and epigenotypes, we identified a GNAS differentially methylated region (DMR) of distinct diagnostic value. This region, GNAS AS2, was hypomethylated in patients with genetic alterations located centromeric but not telomeric of this DMR. The AS2 methylation status was captured by a single probe of the methylation-sensitive multiplex ligation-dependent probe amplification (MS-MLPA) assay utilized to diagnose PHP1B. In human embryonic stem cells, where NESP55 transcription regulates GNAS methylation status on the maternal allele, AS2 methylation depended on 2 imprinting control regions (STX16-ICR and NESP-ICR) essential for NESP55 transcription. These results suggest that the AS2 methylation status in patients with PHP1B reflects the position at which the genetic alteration affects NESP55 transcription during an early embryonic period. Therefore, AS2 methylation levels can enable mechanistic PHP1B categorization based on genotype-epigenotype correlation and, thus, help identify the underlying molecular defect in patients.
    MeSH term(s) Humans ; GTP-Binding Protein alpha Subunits, Gs/genetics ; DNA Methylation ; Pseudohypoparathyroidism/genetics ; Pseudohypoparathyroidism/diagnosis ; Genomic Imprinting ; Alleles ; Chromogranins/genetics
    Chemical Substances GTP-Binding Protein alpha Subunits, Gs (EC 3.6.5.1) ; GNAS protein, human (EC 3.6.1.-) ; Chromogranins
    Language English
    Publishing date 2024-03-08
    Publishing country United States
    Document type Journal Article
    ISSN 2379-3708
    ISSN (online) 2379-3708
    DOI 10.1172/jci.insight.177190
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Editorial: Advances in reproductive biotechnologies in carnivores.

    De Los Reyes, Monica / Songsasen, Nucharin / Herrick, Jason

    Frontiers in veterinary science

    2023  Volume 10, Page(s) 1123368

    Language English
    Publishing date 2023-05-26
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2023.1123368
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Participation of university community members in Health Promoting University (HPU) initiatives.

    Suárez-Reyes, Mónica / Van den Broucke, Stephan

    Frontiers in public health

    2023  Volume 11, Page(s) 1217177

    Abstract: Background: Several universities around the world have adopted the settings approach to health to create a Health Promoting University (HPU) initiative. Health promoting initiatives are built on the values of health promotion, with participation being ... ...

    Abstract Background: Several universities around the world have adopted the settings approach to health to create a Health Promoting University (HPU) initiative. Health promoting initiatives are built on the values of health promotion, with participation being one of the most important. Despite the above, there is little information on how university community members participate in HPU initiatives. This study aims to describe the participation of university community members in HPU initiatives in universities around the world.
    Methods: An online questionnaire was sent to representatives of universities that have implemented a HPU initiative. The questionnaire inquired about the level and nature of participation of university community members (students, professors, and administrative/technical staff) at different levels. Three levels of participation ranged from lower to higher levels were considered: (a) information delivery strategies; (b) consultation strategies and (c) involvement in design, planning and decision-making processes.
    Results: At least the 50% of the universities implemented strategies so that all the members of the community could participate at all levels. Information delivery strategies were the most often used, with students being the main target group. Consultation strategies were aimed mainly at students and professors, whilst professors participated most actively in the design, planning and decision-making.
    Conclusion: Different participation strategies are used in the HPU initiatives. Information delivery strategies, which represent the lowest level of participation, were the most often reported. Higher levels of participation were less used in the HPU initiatives. HPU initiatives should seek for strategies to provide more high-level participation to all university community members.
    MeSH term(s) Humans ; Universities ; Health Promotion ; Referral and Consultation ; Students
    Language English
    Publishing date 2023-08-25
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2711781-9
    ISSN 2296-2565 ; 2296-2565
    ISSN (online) 2296-2565
    ISSN 2296-2565
    DOI 10.3389/fpubh.2023.1217177
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Dynamic Expression of Follicle-Stimulating Hormone and Estrogen mRNA Receptors Associated with microRNAs

    De Los Reyes, Monica / Dettleff, Phillip / Palomino, Jaime / Peralta, Oscar A / Vergara, Ana

    Animals : an open access journal from MDPI

    2024  Volume 14, Issue 2

    Abstract: The genes encoding for estrogen receptor ( ...

    Abstract The genes encoding for estrogen receptor (
    Language English
    Publishing date 2024-01-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani14020214
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Flow cytometric evaluation of canine follicular cell apoptosis during the oestrous cycle

    Palomino, Jaime / De los Reyes, Monica, Dr

    Reproduction in domestic animals. 2022 Feb., v. 57, no. 2

    2022  

    Abstract: Apoptosis is the cellular mechanism of ovarian follicular atresia in mammals; the aim of this study was to examine the apoptosis‐related cyclic changes in follicular cells of different‐sized antral follicles throughout the oestrous cycle in canines. ... ...

    Abstract Apoptosis is the cellular mechanism of ovarian follicular atresia in mammals; the aim of this study was to examine the apoptosis‐related cyclic changes in follicular cells of different‐sized antral follicles throughout the oestrous cycle in canines. Ovaries were collected from 26 adult female dogs (1–4 years) following routine ovariohysterectomy. Antral follicles were classified as small, medium or large antral or preovulatory. The percentage of apoptotic cells was determined flow cytometrically using the terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate (dUTP) DNA nick end labelling (TUNEL) assay. Apoptosis rate was quantified as the percentage of TUNEL‐positive cells on a logarithmic scale. Percentages of TUNEL‐positive cells obtained in the flow cytometric assay were compared among oestrous phases and follicular sizes using analysis of variance. Apoptotic follicles were observed in all types of canine follicles in different cycle phases and stages of development, possibly corresponding to the physiological process of the oestrous cycle. Both the oestrous phase and follicular size significantly influenced the apoptosis rate (p < .05). Apoptosis rate increased significantly (p < .05) as follicular development progressed. Apoptosis rate was the highest in large follicles during the oestrous phase (9.2%; p < .05) and the lowest in small follicles during the anestrus period (1.8%; p < .05). In conclusion, our results demonstrate significant differences in the apoptosis rate during the oestrous cycle related to follicle development in the canine ovary. Furthermore, flow cytometry using the TUNEL assay was found to be an effective method for detecting apoptosis in canine follicles.
    Keywords DNA ; adults ; analysis of variance ; anestrus ; apoptosis ; dogs ; females ; flow cytometry ; follicular atresia ; spaying
    Language English
    Dates of publication 2022-02
    Size p. 165-172.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.14037
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Work/household, transport, and leisure domains account for the sex gap in physical activity in Chile.

    Suárez-Reyes, Mónica / Fernández-Verdejo, Rodrigo

    Frontiers in public health

    2022  Volume 10, Page(s) 1011790

    Abstract: Background: Women usually have lower levels of moderate-vigorous physical activity (MVPA) than men. This sex gap can be accounted for by differences in MVPA in the work/household, transport, and/or leisure domains. Identifying where the differences lay ... ...

    Abstract Background: Women usually have lower levels of moderate-vigorous physical activity (MVPA) than men. This sex gap can be accounted for by differences in MVPA in the work/household, transport, and/or leisure domains. Identifying where the differences lay in a context-specific manner may help close the gap. We aimed to compare MVPA by domain, and the relative contribution of each domain to total MVPA, between men and women in Chile.
    Methods: We analyzed the cross-sectional National Health Survey of Chile 2016-2017 (
    Results: Including all participants, women (vs. men) had lower MVPA (median [25-75th percentile]) for work/household (0 [0-960] vs. 0 [0-5,760] MET × min/week), for transport (360 [0-1,200] vs. 600 [0-1,680] MET × min/week), and for leisure domains (0 [0-0] vs. 0 [0-480] MET × min/week). Including only participants with >0 MET × min/week of MVPA, women (vs. men) had lower mean relative contributions to total MVPA from work/household (31.3 vs. 35.9%) and leisure domains (10.8 vs. 16.3%, respectively), but higher from the transport domain (57.9 vs. 47.8%).
    Conclusion: In Chile, differences in all physical activity domains account for the sex gap in MVPA. Strategies to break job stereotypes, increase opportunities for leisure, and ease active transport are required to encourage MVPA in women.
    MeSH term(s) Adolescent ; Chile ; Cross-Sectional Studies ; Exercise ; Family Characteristics ; Female ; Humans ; Leisure Activities ; Male
    Language English
    Publishing date 2022-09-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2711781-9
    ISSN 2296-2565 ; 2296-2565
    ISSN (online) 2296-2565
    ISSN 2296-2565
    DOI 10.3389/fpubh.2022.1011790
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Elevated Risk of Overweight/Obesity-Related Markers and Low Muscular Fitness in Children Attending Public Schools in Chile.

    Suárez-Reyes, Mónica / Fernández-Verdejo, Rodrigo / Salazar, Gabriela

    International journal of environmental research and public health

    2022  Volume 19, Issue 21

    Abstract: In Chile, children of low socioeconomic status usually attend public schools and have few opportunities to engage in healthy behaviors. This may increase their risk of overweight/obesity and low muscular fitness. Therefore, we aimed to determine the ... ...

    Abstract In Chile, children of low socioeconomic status usually attend public schools and have few opportunities to engage in healthy behaviors. This may increase their risk of overweight/obesity and low muscular fitness. Therefore, we aimed to determine the association between the school type attended with overweight/obesity-related markers and the muscular fitness of children in Chile. We included 1410 children (6-13 years old) attending public, subsidized, or private schools. Overweight/obesity-related markers included BMI Z-scores, waist circumference, and body fat percentage. Muscular fitness assessment included handgrip strength and standing long jump. The odds ratios [95% CI] of overweight/obesity, elevated waist circumference, elevated body fat, low handgrip strength, and low standing long jump were compared between school types. Compared with boys attending public schools, those attending subsidized or private schools had lower odds ratios of low handgrip strength (0.63 [0.42-0.94] and 0.44 [0.25-0.78], respectively). Girls attending subsidized schools, compared with those in public schools, had lower odds of overweight/obesity (0.63 [0.44-0.90]) and of having low handgrip strength (0.51 [0.34-0.78]). Compared with girls in public schools, those attending private schools had lower odds (vs. public schools) of overweight/obesity (0.45 [0.28-0.74]), of having elevated body fat (0.53 [0.29-0.96]), and of having low standing long jump (0.41 [0.21-0.77]). The elevated risk of overweight/obesity-related markers and lower muscular fitness in children, particularly girls, attending public schools increase their current and future disease risk. This suggests that childhood socioeconomic status plays a central role in determining disease risk. Health-promoting interventions specifically focused on children from disadvantaged contexts are required.
    MeSH term(s) Child ; Male ; Female ; Humans ; Adolescent ; Overweight/epidemiology ; Body Mass Index ; Hand Strength ; Chile/epidemiology ; Obesity ; Schools ; Physical Fitness
    Language English
    Publishing date 2022-10-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph192114213
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: New Insights in Canine Reproduction

    De los Reyes, Monica / Songsasen, Nucharin

    Animals. 2021 July 06, v. 11, no. 7

    2021  

    Abstract: Reproduction in canids has been characterized by having some peculiar aspects, such as the extended reproductive cycle and ovulation of immature oocytes [ ... ] ...

    Abstract Reproduction in canids has been characterized by having some peculiar aspects, such as the extended reproductive cycle and ovulation of immature oocytes [...]
    Keywords Canidae ; dogs ; oocytes ; ovulation
    Language English
    Dates of publication 2021-0706
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11072021
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: New Insights in Canine Reproduction.

    De Los Reyes, Monica / Songsasen, Nucharin

    Animals : an open access journal from MDPI

    2021  Volume 11, Issue 7

    Abstract: Reproduction in canids has been characterized by having some peculiar aspects, such as the extended reproductive cycle and ovulation of immature oocytes [ ... ]. ...

    Abstract Reproduction in canids has been characterized by having some peculiar aspects, such as the extended reproductive cycle and ovulation of immature oocytes [...].
    Language English
    Publishing date 2021-07-06
    Publishing country Switzerland
    Document type Editorial
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11072021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Bovine Peripheral Blood-Derived Mesenchymal Stem Cells (PB-MSCs) and Spermatogonial Stem Cells (SSCs) Display Contrasting Expression Patterns of Pluripotency and Germ Cell Markers under the Effect of Sertoli Cell Conditioned Medium.

    Segunda, Moisés N / Díaz, Carlos / Torres, Cristian G / Parraguez, Víctor H / De Los Reyes, Mónica / Peralta, Oscar A

    Animals : an open access journal from MDPI

    2024  Volume 14, Issue 5

    Abstract: In vitro gamete derivation has been proposed as an interesting strategy for treatment of infertility, improvement of genetic traits, and conservation of endangered animals. Spermatogonial stem cells (SSCs) are primary candidates for in vitro gamete ... ...

    Abstract In vitro gamete derivation has been proposed as an interesting strategy for treatment of infertility, improvement of genetic traits, and conservation of endangered animals. Spermatogonial stem cells (SSCs) are primary candidates for in vitro gamete derivation; however, recently, mesenchymal stem cells (MSCs) have also been proposed as candidates for germ cell (GCs) differentiation mainly due to their transdifferentiating capacity. The objective of the present study was to compare the potential for GC differentiation of bovine peripheral blood-derived MSCs (PB-MSCs) and SSCs under the effect of conditioned medium (CM) derived from Sertoli cells (SCs/CM). Samples were collected every 7 days for 21 days and analyzed for pluripotent, GC, and MSC marker expression. The absence of OCT4 and the increased (
    Language English
    Publishing date 2024-03-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani14050803
    Database MEDical Literature Analysis and Retrieval System OnLINE

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