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  1. Article ; Online: High-throughput light sheet imaging of adult and larval

    Rahimpouresfahani, Faraz / Tabatabaei, Nima / Rezai, Pouya

    RSC advances

    2024  Volume 14, Issue 1, Page(s) 626–639

    Abstract: Recent advancements at the interface of microfluidics technology and light sheet fluorescence microscopy have opened the door for high-throughput and high-content investigation ... ...

    Abstract Recent advancements at the interface of microfluidics technology and light sheet fluorescence microscopy have opened the door for high-throughput and high-content investigation of
    Language English
    Publishing date 2024-01-02
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d3ra06323b
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  2. Article: Microfluidic Droplet-Generation Device with Flexible Walls.

    Yazdanparast, Sajad / Rezai, Pouya / Amirfazli, Alidad

    Micromachines

    2023  Volume 14, Issue 9

    Abstract: Controlling droplet sizes is one of the most important aspects of droplet generators used in biomedical research, drug discovery, high-throughput screening, and emulsion manufacturing applications. This is usually achieved by using multiple devices that ... ...

    Abstract Controlling droplet sizes is one of the most important aspects of droplet generators used in biomedical research, drug discovery, high-throughput screening, and emulsion manufacturing applications. This is usually achieved by using multiple devices that are restricted in their range of generated droplet sizes. In this paper, a co-flow microfluidic droplet-generation device with flexible walls was developed such that the width of the continuous (C)-phase channel around the dispersed (D)-phase droplet-generating needle can be adjusted on demand. This actuation mechanism allowed for the adjustment of the C-phase flow velocity, hence providing modulated viscous forces to manipulate droplet sizes in a single device. Two distinct droplet-generation regimes were observed at low D-phase Weber numbers, i.e., a dripping regime at high- and medium-channel widths and a plug regime at low-channel widths. The effect of channel width on droplet size was investigated in the dripping regime under three modes of constant C-phase flow rate, velocity, and Capillary number. Reducing the channel width at a constant C-phase flow rate had the most pronounced effect on producing smaller droplets. This effect can be attributed to the combined influences of the wall effect and increased C-phase velocity, leading to a greater impact on droplet size due to the intensified viscous force. Droplet sizes in the range of 175-913 µm were generated; this range was ~2.5 times wider than the state of the art, notably using a single microfluidic device. Lastly, an empirical model based on Buckingham's Pi theorem was developed to predict the size of droplets based on channel width and height as well as the C-phase Capillary and Reynolds numbers.
    Language English
    Publishing date 2023-09-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2620864-7
    ISSN 2072-666X
    ISSN 2072-666X
    DOI 10.3390/mi14091770
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  3. Article ; Online: Recent advances in biosensors for real time monitoring of pH, temperature, and oxygen in chronic wounds.

    Youssef, Khaled / Ullah, Asad / Rezai, Pouya / Hasan, Anwarul / Amirfazli, Alidad

    Materials today. Bio

    2023  Volume 22, Page(s) 100764

    Abstract: Chronic wounds are among the major healthcare issues affecting millions of people worldwide with high rates of morbidity, losses of limbs and mortality. Microbial infection in wounds is a severe problem that can impede healing of chronic wounds. Accurate, ...

    Abstract Chronic wounds are among the major healthcare issues affecting millions of people worldwide with high rates of morbidity, losses of limbs and mortality. Microbial infection in wounds is a severe problem that can impede healing of chronic wounds. Accurate, timely and early detection of infections, and real time monitoring of various wound healing biomarkers related to infection can be significantly helpful in the treatment and care of chronic wounds. However, clinical methodologies of periodic assessment and care of wounds require physical visit to wound care clinics or hospitals and time-consuming frequent replacement of wound dressing patches, which also often adversely affect the healing process. Besides, frequent replacements of wound dressings are highly expensive, causing a huge amount of burden on the national health care systems. Smart bandages have emerged to provide
    Language English
    Publishing date 2023-08-19
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2590-0064
    ISSN (online) 2590-0064
    DOI 10.1016/j.mtbio.2023.100764
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  4. Article ; Online: Fluorescent bacteria detection in water using cell imprinted polymer (CIP) coated microparticles in a magnetophoretic microfluidic device.

    Doostmohammadi, Ali / Youssef, Khaled / Akhtarian, Shiva / Kraft, Garrett / Rezai, Pouya

    Talanta

    2023  Volume 268, Issue Pt 1, Page(s) 125290

    Abstract: Molecular imprinting has advanced towards synthesizing whole-cell imprints of microorganisms such as bacteria on various sensor surfaces including wire electrodes, quartz crystal microbalances, and microparticles (MPs). We recently introduced cell- ... ...

    Abstract Molecular imprinting has advanced towards synthesizing whole-cell imprints of microorganisms such as bacteria on various sensor surfaces including wire electrodes, quartz crystal microbalances, and microparticles (MPs). We recently introduced cell-imprinted polymers (CIPs) coated on MPs, called CIP-MPs, for bacteria recovery from water. In this paper, we have advanced towards rapid fluorometric Escherichia coli (E. coli) detection by applying fluorescent magnetic CIP-MPs, which were captured by soft ferromagnetic microstructures integrated into a microfluidic channel. The shape of ferromagnetic microstructures was optimized numerically to enhance the magnetophoretic accumulation of CIP-MPs in the microchannel. The device was then fabricated and the flow rate required to enhance bacteria capturing efficiency by CIP-MPs was determined. Decreasing the flow rate reduced the flow-induced drag and increased the interactions between the bacteria and imprinted CIP cavities. Fluorescent imaging of the accumulated CIP-MPs was done before and after bacteria capturing to quantify the changes in the fluorescence intensity as E. coli cells were captured by CIP-MPs in the microchannel. The dose-response curve of the sensor at 0-10
    MeSH term(s) Water ; Polymers/chemistry ; Escherichia coli ; Bacteria ; Limit of Detection ; Molecular Imprinting ; Coloring Agents
    Chemical Substances Water (059QF0KO0R) ; Polymers ; Coloring Agents
    Language English
    Publishing date 2023-10-14
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500969-5
    ISSN 1873-3573 ; 0039-9140
    ISSN (online) 1873-3573
    ISSN 0039-9140
    DOI 10.1016/j.talanta.2023.125290
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  5. Article ; Online: Low-Cost Resistive Microfluidic Salinity Sensor for High-Precision Detection of Drinking Water Salt Levels.

    Farshchi Heydari, Mohammad Javad / Tabatabaei, Nima / Rezai, Pouya

    ACS omega

    2022  Volume 7, Issue 18, Page(s) 15529–15539

    Abstract: Rapid, inexpensive, and precise water salinity testing remains indispensable in water quality monitoring applications. Despite many sensors and commercialized devices to monitor seawater salinity, salt detection and quantification at very low levels of ... ...

    Abstract Rapid, inexpensive, and precise water salinity testing remains indispensable in water quality monitoring applications. Despite many sensors and commercialized devices to monitor seawater salinity, salt detection and quantification at very low levels of drinking water (below 120 ppm) have been overlooked. In this paper, we report on optimization of a low-cost microfluidic sensor to measure water salinity in the range of 1-120 ppm. The proposed design employs two copper microbridge wires suspended orthogonally in a PDMS microchannel to measure salinity based on the electrical resistance between the wires. The preliminary design of the sensor microchannel with a rectangular cross-section width (
    Language English
    Publishing date 2022-04-26
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.2c00268
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  6. Article: Conventional and microfluidic methods for airborne virus isolation and detection

    Krokhine, Sophie / Torabi, Hadis / Doostmohammadi, Ali / Rezai, Pouya

    Colloids and surfaces. 2021 Oct., v. 206

    2021  

    Abstract: With the COVID-19 pandemic, the threat of infectious diseases to public health and safety has become much more apparent. Viral, bacterial and fungal diseases have led to the loss of millions of lives, especially in the developing world. Diseases caused ... ...

    Abstract With the COVID-19 pandemic, the threat of infectious diseases to public health and safety has become much more apparent. Viral, bacterial and fungal diseases have led to the loss of millions of lives, especially in the developing world. Diseases caused by airborne viruses like SARS-CoV-2 are difficult to control, as these viruses are easily transmissible and can circulate in the air for hours. To contain outbreaks of viruses such as SARS-CoV-2 and institute targeted precautions, it is important to detect them in air and understand how they infect their targets. Point-of-care (PoC) diagnostics and point-of-need (PoN) detection methods are necessary to rapidly test patient and environmental samples, so precautions can immediately be applied. Traditional benchtop detection methods such as ELISA, PCR and culture are not suitable for PoC and PoN monitoring, because they can take hours to days and require specialized equipment. Microfluidic devices can be made at low cost to perform such assays rapidly and at the PoN. They can also be integrated with air- and liquid-based sampling technologies to capture and analyze viruses from air and body fluids. Here, conventional and microfluidic virus detection methods are reviewed and compared. The use of air sampling devices to capture and concentrate viruses is discussed first, followed by a review of analysis methods such as immunoassays, RT-PCR and isothermal amplification in conventional and microfluidic platforms. This review provides an overview of the capabilities of microfluidics in virus handling and detection, which will be useful to infectious disease researchers, biomedical engineers, and public health agencies.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; air ; diagnostic techniques ; equipment ; fungi ; microfluidic technology ; patients ; point-of-care systems ; public health ; viruses
    Language English
    Dates of publication 2021-10
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500523-9
    ISSN 1873-4367 ; 0927-7765
    ISSN (online) 1873-4367
    ISSN 0927-7765
    DOI 10.1016/j.colsurfb.2021.111962
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  7. Article: High-speed label-free confocal microscopy of

    Rashtchian, Sadaf / Youssef, Khaled / Rezai, Pouya / Tabatabaei, Nima

    Biomedical optics express

    2021  Volume 12, Issue 6, Page(s) 3607–3618

    Abstract: Caenorhabditis ... ...

    Abstract Caenorhabditis elegans
    Language English
    Publishing date 2021-05-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.427685
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  8. Article ; Online: Microfluidic devices for embryonic and larval zebrafish studies.

    Khalili, Arezoo / Rezai, Pouya

    Briefings in functional genomics

    2019  Volume 18, Issue 6, Page(s) 419–432

    Abstract: Zebrafish or Danio rerio is an established model organism for studying the genetic, neuronal and behavioral bases of diseases and for toxicology and drug screening. The embryonic and larval stages of zebrafish have been used extensively in fundamental ... ...

    Abstract Zebrafish or Danio rerio is an established model organism for studying the genetic, neuronal and behavioral bases of diseases and for toxicology and drug screening. The embryonic and larval stages of zebrafish have been used extensively in fundamental and applied research due to advantages offered such as body transparency, small size, low cost of cultivation and high genetic homology with humans. However, the manual experimental methods used for handling and investigating this organism are limited due to their low throughput, labor intensiveness and inaccuracy in delivering external stimuli to the zebrafish while quantifying various neuronal and behavioral responses. Microfluidic and lab-on-a-chip devices have emerged as ideal technologies to overcome these challenges. In this review paper, the current microfluidic approaches for investigation of behavior and neurobiology of zebrafish at embryonic and larval stages will be reviewed. Our focus will be to provide an overview of the microfluidic methods used to manipulate (deliver and orient), immobilize and expose or inject zebrafish embryos or larvae, followed by quantification of their responses in terms of neuron activities and movement. We will also provide our opinion in terms of the direction that the field of zebrafish microfluidics is heading toward in the area of biomedical engineering.
    MeSH term(s) Animals ; Biomedical Engineering/methods ; Biomedical Engineering/trends ; Embryo, Nonmammalian ; Lab-On-A-Chip Devices ; Larva ; Microchip Analytical Procedures/methods ; Microchip Analytical Procedures/trends ; Models, Animal ; Zebrafish/embryology ; Zebrafish/growth & development ; Zebrafish/physiology
    Language English
    Publishing date 2019-04-29
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2540916-5
    ISSN 2041-2657 ; 2041-2649 ; 2041-2647
    ISSN (online) 2041-2657
    ISSN 2041-2649 ; 2041-2647
    DOI 10.1093/bfgp/elz006
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    In stock of ZB MED Cologne/Königswinter

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  9. Article ; Online: Microfluidic Sensor Based on Cell-Imprinted Polymer-Coated Microwires for Conductometric Detection of Bacteria in Water.

    Akhtarian, Shiva / Doostmohammadi, Ali / Archonta, Daphne-Eleni / Kraft, Garrett / Brar, Satinder Kaur / Rezai, Pouya

    Biosensors

    2023  Volume 13, Issue 10

    Abstract: The rapid, inexpensive, and on-site detection of bacterial contaminants using highly sensitive and specific microfluidic sensors is attracting substantial attention in water quality monitoring applications. Cell-imprinted polymers (CIPs) have emerged as ... ...

    Abstract The rapid, inexpensive, and on-site detection of bacterial contaminants using highly sensitive and specific microfluidic sensors is attracting substantial attention in water quality monitoring applications. Cell-imprinted polymers (CIPs) have emerged as robust, cost-effective, and versatile recognition materials with selective binding sites for capturing whole bacteria. However, electrochemical transduction of the binding event to a measurable signal within a microfluidic device to develop easy-to-use, compact, portable, durable, and affordable sensors remains a challenge. For this paper, we employed CIP-functionalized microwires (CIP-MWs) with an affinity towards
    MeSH term(s) Microfluidics ; Escherichia coli ; Biosensing Techniques/methods ; Polymers/chemistry ; Binding Sites
    Chemical Substances Polymers
    Language English
    Publishing date 2023-10-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios13100943
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  10. Article ; Online: Low-cost optofluidic add-on enables rapid selective plane illumination microscopy of C. elegans with a conventional wide-field microscope.

    Behrouzi, Mehran / Youssef, Khaled / Rezai, Pouya / Tabatabaei, Nima

    Journal of biomedical optics

    2021  Volume 26, Issue 12

    Abstract: Significance: Selective plane illumination microscopy (SPIM) is an emerging fluorescent imaging technique suitable for noninvasive volumetric imaging of C. elegans. These promising microscopy systems, however, are scarce in academic and research ... ...

    Abstract Significance: Selective plane illumination microscopy (SPIM) is an emerging fluorescent imaging technique suitable for noninvasive volumetric imaging of C. elegans. These promising microscopy systems, however, are scarce in academic and research institutions due to their high cost and technical complexities. Simple and low-cost solutions that enable conversion of commonplace wide-field microscopes to rapid SPIM platforms promote widespread adoption of SPIM by biologist for studying neuronal expressions of C. elegans.
    Aim: We sought to develop a simple and low-cost optofluidic add-on device that enables rapid and immobilization-free volumetric SPIM imaging of C. elegans with conventional fluorescent microscopes.
    Approach: A polydimethylsiloxane (PDMS)-based device with integrated optical and fluidic elements was developed as a low-cost and miniaturized SPIM add-on for the conventional wide-field microscope. The developed optofluidic chip contained an integrated PDMS cylindrical lens for on-chip generation of the light-sheet across a microchannel. Cross-sectional SPIM images of C. elegans were continuously acquired by the native objective of microscope as worms flowed in an L-shape microchannel and through the light sheet.
    Results: On-chip SPIM imaging of C. elegans strains demonstrated possibility of visualizing the entire neuronal system in few seconds at single-neuron resolution, with high contrast and without worm immobilization. Volumetric visualization of neuronal system from the acquired cross-sectional two-dimensional images is also demonstrated, enabling the standard microscope to acquire three-dimensional fluorescent images of C. elegans. The full-width at half-maximum width of the point spread function was measured as 1.1 and 2.4  μm in the lateral and axial directions, respectively.
    Conclusion: The developed low-cost optofluidic device is capable of continuous SPIM imaging of C. elegans model organism with a conventional fluorescent microscope, at high speed, and with single neuron resolution.
    MeSH term(s) Animals ; Caenorhabditis elegans ; Cross-Sectional Studies ; Lighting ; Microscopy
    Language English
    Publishing date 2021-12-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1309154-2
    ISSN 1560-2281 ; 1083-3668
    ISSN (online) 1560-2281
    ISSN 1083-3668
    DOI 10.1117/1.JBO.26.12.126501
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4699: Show issues Location:
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