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Article ; Online: Esophageal Achalasia: Diagnostic Evaluation.

Riccio, Federica / Costantini, Mario / Salvador, Renato

2022 Volume 46, Issue 7, Page(s) 1516–1521

Abstract: A precise diagnosis is key to the successful treatment of achalasia. Barium swallow, upper endoscopy and high-resolution manometry provide the necessary information about a patient's anatomy, absence of other diseases, and type of achalasia (I, II, III). ...

Abstract	A precise diagnosis is key to the successful treatment of achalasia. Barium swallow, upper endoscopy and high-resolution manometry provide the necessary information about a patient's anatomy, absence of other diseases, and type of achalasia (I, II, III). High-resolution manometry also has prognostic value, the best results of treatment being obtained in type II achalasia according to the Chicago classification. Abdominal CT scanning and endoscopic ultrasound might be warranted if an underlying malignancy is suspected.
MeSH term(s)	Esophageal Achalasia/diagnostic imaging ; Humans ; Manometry/methods ; Tomography, X-Ray Computed
Language	English
Publishing date	2022-02-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	224043-9
ISSN	1432-2323 ; 0364-2313
ISSN (online)	1432-2323
ISSN	0364-2313
DOI	10.1007/s00268-022-06483-3
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Article ; Online: Enrichment of human embryonic stem cell-derived V3 interneurons using an Nkx2-2 gene-specific reporter.

Berzanskyte, Ieva / Riccio, Federica / Machado, Carolina Barcellos / Bradbury, Elizabeth J / Lieberam, Ivo

Scientific reports

2023 Volume 13, Issue 1, Page(s) 2008

Abstract: V3 spinal interneurons are a key element of the spinal circuits, which control motor function. However, to date, there are no effective ways of deriving a pure V3 population from human pluripotent stem cells. Here, we report a method for differentiation ... ...

Abstract	V3 spinal interneurons are a key element of the spinal circuits, which control motor function. However, to date, there are no effective ways of deriving a pure V3 population from human pluripotent stem cells. Here, we report a method for differentiation and isolation of spinal V3 interneurons, combining extrinsic factor-mediated differentiation and magnetic activated cell sorting. We found that differentiation of V3 progenitors can be enhanced with a higher concentration of Sonic Hedgehog agonist, as well as culturing cells in 3D format. To enable V3 progenitor purification from mixed differentiation cultures, we developed a transgene reporter, with a part of the regulatory region of V3-specific gene Nkx2-2 driving the expression of a membrane marker CD14. We found that in human cells, NKX2-2 initially exhibited co-labelling with motor neuron progenitor marker, but V3 specificity emerged as the differentiation culture progressed. At these later differentiation timepoints, we were able to enrich V3 progenitors labelled with CD14 to ~ 95% purity, and mature them to postmitotic V3 interneurons. This purification tool for V3 interneurons will be useful for in vitro disease modeling, studies of normal human neural development and potential cell therapies for disorders of the spinal cord.
MeSH term(s)	Humans ; Cell Differentiation ; Hedgehog Proteins/metabolism ; Human Embryonic Stem Cells ; Interneurons/metabolism ; Motor Neurons/metabolism ; Spinal Cord/metabolism ; Homeobox Protein Nkx-2.2/genetics
Chemical Substances	Hedgehog Proteins ; Homeobox Protein Nkx-2.2
Language	English
Publishing date	2023-02-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-29165-z
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Article ; Online: A scalable human iPSC-based neuromuscular disease model on suspended biobased elastomer nanofiber scaffolds.

Cheesbrough, Aimee / Harley, Peter / Riccio, Federica / Wu, Lei / Song, Wenhui / Lieberam, Ivo

Biofabrication

2023 Volume 15, Issue 4

Abstract: Many devastating neuromuscular diseases currently lack effective treatments. This is in part due to a lack of drug discovery platforms capable of assessing complex human neuromuscular disease phenotypes in a scalable manner. A major obstacle has been ... ...

Abstract	Many devastating neuromuscular diseases currently lack effective treatments. This is in part due to a lack of drug discovery platforms capable of assessing complex human neuromuscular disease phenotypes in a scalable manner. A major obstacle has been generating scaffolds to stabilise mature contractile myofibers in a multi-well assay format amenable to high content image (HCI) analysis. This study describes the development of a scalable human induced pluripotent stem cell (iPSC)-neuromuscular disease model, whereby suspended elastomer nanofibers support long-term stability, alignment, maturation, and repeated contractions of iPSC-myofibers, innervated by iPSC-motor neurons in 96-well assay plates. In this platform, optogenetic stimulation of the motor neurons elicits robust myofiber-contractions, providing a functional readout of neuromuscular transmission. Additionally, HCI analysis provides rapid and automated quantification of axonal outgrowth, myofiber morphology, and neuromuscular synapse number and morphology. By incorporating amyotrophic lateral sclerosis (ALS)-related TDP-43
MeSH term(s)	Humans ; Amyotrophic Lateral Sclerosis ; Induced Pluripotent Stem Cells ; Nanofibers ; Elastomers ; Neuromuscular Diseases
Chemical Substances	Elastomers
Language	English
Publishing date	2023-09-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2500944-8
ISSN	1758-5090 ; 1758-5082
ISSN (online)	1758-5090
ISSN	1758-5082
DOI	10.1088/1758-5090/acf39e
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Article ; Online: When a Friend Becomes Your Enemy: Natural Killer Cells in Atherosclerosis and Atherosclerosis-Associated Risk Factors.

Palano, Maria Teresa / Cucchiara, Martina / Gallazzi, Matteo / Riccio, Federica / Mortara, Lorenzo / Gensini, Gian Franco / Spinetti, Gaia / Ambrosio, Giuseppe / Bruno, Antonino

Frontiers in immunology

2022 Volume 12, Page(s) 798155

Abstract: Atherosclerosis (ATS), the change in structure and function of arteries with associated lesion formation and altered blood flow, is the leading cause of cardiovascular disease, the number one killer worldwide. Beyond dyslipidemia, chronic inflammation, ... ...

Abstract	Atherosclerosis (ATS), the change in structure and function of arteries with associated lesion formation and altered blood flow, is the leading cause of cardiovascular disease, the number one killer worldwide. Beyond dyslipidemia, chronic inflammation, together with aberrant phenotype and function of cells of both the innate and adaptive immune system, are now recognized as relevant contributors to atherosclerosis onset and progression. While the role of macrophages and T cells in atherosclerosis has been addressed in several studies, Natural Killer cells (NKs) represent a poorly explored immune cell type, that deserves attention, due to NKs' emerging contribution to vascular homeostasis. Furthermore, the possibility to re-polarize the immune system has emerged as a relevant tool to design new therapies, with some succesfull exmples in the field of cancer immunotherapy. Thus, a deeper knowledge of NK cell pathophysiology in the context of atherosclerosis and atherosclerosis-associated risk factors could help developing new preventive and treatment strategies, and decipher the complex scenario/history from "the risk factors for atherosclerosis" Here, we review the current knowledge about NK cell phenotype and activities in atherosclerosis and selected atherosclerosis risk factors, namely type-2 diabetes and obesity, and discuss the related NK-cell oriented environmental signals.
MeSH term(s)	Adaptive Immunity/immunology ; Animals ; Atherosclerosis/immunology ; Cell Movement/immunology ; Cytokines/immunology ; Cytokines/metabolism ; Diabetes Mellitus, Type 2/immunology ; Homeostasis/immunology ; Humans ; Immunity, Innate/immunology ; Inflammation/immunology ; Inflammation/metabolism ; Killer Cells, Natural/immunology ; Models, Immunological ; Obesity/immunology ; Risk Factors
Chemical Substances	Cytokines
Language	English
Publishing date	2022-01-13
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.798155
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Article: Transcription Factor Activation Profiles (TFAP) identify compounds promoting differentiation of Acute Myeloid Leukemia cell lines.

Riccio, Federica / Micarelli, Elisa / Secci, Riccardo / Giuliani, Giulio / Vumbaca, Simone / Massacci, Giorgia / Castagnoli, Luisa / Fuoco, Claudia / Cesareni, Gianni

Cell death discovery

2022 Volume 8, Issue 1, Page(s) 16

Abstract: Repurposing of drugs for new therapeutic use has received considerable attention for its potential to limit time and cost of drug development. Here we present a new strategy to identify chemicals that are likely to promote a desired phenotype. We used ... ...

Abstract	Repurposing of drugs for new therapeutic use has received considerable attention for its potential to limit time and cost of drug development. Here we present a new strategy to identify chemicals that are likely to promote a desired phenotype. We used data from the Connectivity Map (CMap) to produce a ranked list of drugs according to their potential to activate transcription factors that mediate myeloid differentiation of leukemic progenitor cells. To validate our strategy, we tested the in vitro differentiation potential of candidate compounds using the HL-60 human cell line as a myeloid differentiation model. Ten out of 22 compounds, which were ranked high in the inferred list, were confirmed to promote significant differentiation of HL-60. These compounds may be considered candidate for differentiation therapy. The method that we have developed is versatile and it can be adapted to different drug repurposing projects.
Language	English
Publishing date	2022-01-10
Publishing country	United States
Document type	Journal Article
ISSN	2058-7716
ISSN	2058-7716
DOI	10.1038/s41420-021-00811-7
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Article ; Online: Biobased Elastomer Nanofibers Guide Light-Controlled Human-iPSC-Derived Skeletal Myofibers.

Cheesbrough, Aimee / Sciscione, Fabiola / Riccio, Federica / Harley, Peter / R'Bibo, Lea / Ziakas, Georgios / Darbyshire, Arnold / Lieberam, Ivo / Song, Wenhui

Advanced materials (Deerfield Beach, Fla.)

2022 Volume 34, Issue 18, Page(s) e2110441

Abstract: Generating skeletal muscle tissue that mimics the cellular alignment, maturation, and function of native skeletal muscle is an ongoing challenge in disease modeling and regenerative therapies. Skeletal muscle cultures require extracellular guidance and ... ...

Abstract	Generating skeletal muscle tissue that mimics the cellular alignment, maturation, and function of native skeletal muscle is an ongoing challenge in disease modeling and regenerative therapies. Skeletal muscle cultures require extracellular guidance and mechanical support to stabilize contractile myofibers. Existing microfabrication-based solutions are limited by complex fabrication steps, low throughput, and challenges in measuring dynamic contractile function. Here, the synthesis and characterization of a new biobased nanohybrid elastomer, which is electrospun into aligned nanofiber sheets to mimic the skeletal muscle extracellular matrix, is presented. The polymer exhibits remarkable hyperelasticity well-matched to that of native skeletal muscle (≈11-50 kPa), with ultimate strain ≈1000%, and elastic modulus ≈25 kPa. Uniaxially aligned nanofibers guide myoblast alignment, enhance sarcomere formation, and promote a ≈32% increase in myotube fusion and ≈50% increase in myofiber maturation. The elastomer nanofibers stabilize optogenetically controlled human induced pluripotent stem cell derived skeletal myofibers. When activated by blue light, the myofiber-nanofiber hybrid constructs maintain a significantly higher (>200%) contraction velocity and specific force (>280%) compared to conventional culture methods. The engineered myofibers exhibit a power density of ≈35 W m
MeSH term(s)	Cell Differentiation ; Elastomers ; Humans ; Induced Pluripotent Stem Cells ; Muscle Fibers, Skeletal ; Muscle, Skeletal ; Nanofibers ; Tissue Engineering/methods ; Tissue Scaffolds
Chemical Substances	Elastomers
Language	English
Publishing date	2022-03-31
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1474949-X
ISSN	1521-4095 ; 0935-9648
ISSN (online)	1521-4095
ISSN	0935-9648
DOI	10.1002/adma.202110441
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Article ; Online: Motion of VAPB molecules reveals ER-mitochondria contact site subdomains.

Obara, Christopher J / Nixon-Abell, Jonathon / Moore, Andrew S / Riccio, Federica / Hoffman, David P / Shtengel, Gleb / Xu, C Shan / Schaefer, Kathy / Pasolli, H Amalia / Masson, Jean-Baptiste / Hess, Harald F / Calderon, Christopher P / Blackstone, Craig / Lippincott-Schwartz, Jennifer

Nature

2024 Volume 626, Issue 7997, Page(s) 169–176

Abstract: To coordinate cellular physiology, eukaryotic cells rely on the rapid exchange of molecules at specialized organelle-organelle contact ... ...

Abstract	To coordinate cellular physiology, eukaryotic cells rely on the rapid exchange of molecules at specialized organelle-organelle contact sites
MeSH term(s)	Humans ; Amyotrophic Lateral Sclerosis/genetics ; Endoplasmic Reticulum/chemistry ; Endoplasmic Reticulum/metabolism ; Endoplasmic Reticulum/ultrastructure ; Mitochondria/chemistry ; Mitochondria/metabolism ; Mitochondria/ultrastructure ; Mitochondrial Membranes/chemistry ; Mitochondrial Membranes/metabolism ; Mitochondrial Membranes/ultrastructure ; Signal Transduction ; Vesicular Transport Proteins/genetics ; Vesicular Transport Proteins/metabolism ; Vesicular Transport Proteins/ultrastructure ; Microscopy, Electron ; Imaging, Three-Dimensional ; Binding Sites ; Diffusion ; Time Factors ; Mutation ; Homeostasis ; Movement
Chemical Substances	VAPB protein, human ; Vesicular Transport Proteins
Language	English
Publishing date	2024-01-24
Publishing country	England
Document type	Journal Article
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/s41586-023-06956-y
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Zs.A 26: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 9: Show issues
Zs.MO 244: Show issues

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Article: Surgical oncological emergencies in octogenarian patients.

Friziero, Alberto / Sperti, Cosimo / Riccio, Federica / Zuin, Irene Sole / Vallese, Lorenzo / Serafini, Simone / Amico, Alessandra / Valli, Valeria / Da Re, Chiara / Baldan, Nicola / Valmasoni, Michele / Da Dalt, Gianfranco

Frontiers in oncology

2023 Volume 13, Page(s) 1268190

Abstract: Background: Surgical oncological emergencies represent a frequent challenge in acute settings, with postoperative courses characterized by high morbidity and mortality. An accurate selection of patients who could benefit from surgery is essential to ... ...

Abstract	Background: Surgical oncological emergencies represent a frequent challenge in acute settings, with postoperative courses characterized by high morbidity and mortality. An accurate selection of patients who could benefit from surgery is essential to avoid unnecessary invasive treatment. In this study, we tried to determine if advanced age (>80 years) represents a risk factor for negative short-term outcome in patients undergoing emergency surgery for acute abdominal oncological illness. Methods: We retrospectively analyzed the records of patients who underwent emergency oncological surgery at the Department of Acute Care Surgery of Padua General Hospital from January 2018 to December 2022. One hundred two cancer patients were included in the study. Among them, 42 were aged ≥80 years (41%). Multiple preoperative and postoperative parameters were recorded, and the follow-up period was at least 90 days. Multivariate logistic regression analyses were used to identify factors associated with short-term postoperative outcomes. Results: In the octogenarian group, 30-day mortality was 11% vs. 9.5% in the younger group [p = not significant ( Conclusion: Advanced age is not a risk factor for negative outcomes in surgical oncological emergencies. Therefore, surgical options should be considered in octogenarians with oncological emergencies and acceptable clinical conditions. Serum albumin levels and LDH can help predict the postoperative outcome after surgery for oncological emergencies.
Language	English
Publishing date	2023-11-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1268190
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Article ; Online: Aberrant axon initial segment plasticity and intrinsic excitability of ALS hiPSC motor neurons.

Harley, Peter / Kerins, Caoimhe / Gatt, Ariana / Neves, Guilherme / Riccio, Federica / Machado, Carolina Barcellos / Cheesbrough, Aimee / R'Bibo, Lea / Burrone, Juan / Lieberam, Ivo

Cell reports

2023 Volume 42, Issue 12, Page(s) 113509

Abstract: Dysregulated neuronal excitability is a hallmark of amyotrophic lateral sclerosis (ALS). We sought to investigate how functional changes to the axon initial segment (AIS), the site of action potential generation, could impact neuronal excitability in ALS ...

Abstract	Dysregulated neuronal excitability is a hallmark of amyotrophic lateral sclerosis (ALS). We sought to investigate how functional changes to the axon initial segment (AIS), the site of action potential generation, could impact neuronal excitability in ALS human induced pluripotent stem cell (hiPSC) motor neurons. We find that early TDP-43 and C9orf72 hiPSC motor neurons show an increase in the length of the AIS and impaired activity-dependent AIS plasticity that is linked to abnormal homeostatic regulation of neuronal activity and intrinsic hyperexcitability. In turn, these hyperactive neurons drive increased spontaneous myofiber contractions of in vitro hiPSC motor units. In contrast, late hiPSC and postmortem ALS motor neurons show AIS shortening, and hiPSC motor neurons progress to hypoexcitability. At a molecular level, aberrant expression of the AIS master scaffolding protein ankyrin-G and AIS-specific voltage-gated sodium channels mirror these dynamic changes in AIS function and excitability. Our results point toward the AIS as an important site of dysfunction in ALS motor neurons.
MeSH term(s)	Humans ; Axon Initial Segment/metabolism ; Amyotrophic Lateral Sclerosis/metabolism ; Induced Pluripotent Stem Cells/metabolism ; Motor Neurons/metabolism ; Action Potentials/physiology
Language	English
Publishing date	2023-11-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2023.113509
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Article ; Online: Antireflux Surgery's Lifespan: 20 Years After Laparoscopic Fundoplication.

Salvador, Renato / Vittori, Arianna / Capovilla, Giovanni / Riccio, Federica / Nezi, Giulia / Forattini, Francesca / Provenzano, Luca / Nicoletti, Loredana / Moletta, Lucia / Costantini, Andrea / Valmasoni, Michele / Costantini, Mario

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract

2023 Volume 27, Issue 11, Page(s) 2325–2335

MeSH term(s)	Humans ; Fundoplication ; Longevity ; Gastroesophageal Reflux/surgery ; Stomach ; Laparoscopy ; Hernia, Hiatal/surgery ; Treatment Outcome
Language	English
Publishing date	2023-08-14
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2012365-6
ISSN	1873-4626 ; 1934-3213 ; 1091-255X
ISSN (online)	1873-4626 ; 1934-3213
ISSN	1091-255X
DOI	10.1007/s11605-023-05797-4
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Zs.A 5207: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 354: Show issues

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